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The map label for this gene is thyA [H]

Identifier: 150401473

GI number: 150401473

Start: 1082918

End: 1083586

Strand: Direct

Name: thyA [H]

Synonym: Maeo_1049

Alternate gene names: 150401473

Gene position: 1082918-1083586 (Clockwise)

Preceding gene: 150401464

Following gene: 150401474

Centisome position: 69.0

GC content: 32.14

Gene sequence:

>669_bases
ATGTATTGTATCAAGAAACCTTCCGTCAGGGCAAGTTATGAAGAGTTAGTTAAAGAAGTATTGAAAAACGGGGAAGATAT
GATAACAGAAGATGGGCAAAAATGTAGGGAGATAAGAAACACAGTAATAGAAATTACAAATCCGAAATTAAAAAGTATAA
GCCCTAAATACCCCCTTGGAAAAAAGGCAGTAGAGCAATACACCAATAATTTATTGTATGGCTCTGAAAATGTTTTTTCT
TATGATTATCATTCTCGGTTATTCAAATATCCAGTAAATAATAAAGAAATAAACCAAATCGATTTTATTGTTGAGAAATT
AAAAGAACAGCAAAATTCAAGGCGATGCGTTGCTATTACATGGCAACCCAATATTGATATTGAAGTAAGTAATAAAGACC
ATGGTTCTGTTCCATGTTTGCAATTTGTTCAATTTCTTATACGAGATAATAAGCTATATCAGACCGTATTATTTAGAAGT
AACGATCTGGCAGTCGCTTTTGTAAGTAATGCATTAGGATTGATTGCATTAGGTGAAATGGTAGCGGAAAAAGTCGGCGT
TGATTATGGAACATATACACACCACAGCGTTTCAATGCACATCTATATTGACAGGGATAGAGATTATATTGAAAAGTATT
TTCCTGAATCTTTAAAATATTTAGAATAA

Upstream 100 bases:

>100_bases
ATGCAAAATAATATAAAATTAGCAAAAATAAACAATATTATAAAATGCGTTGGGACGGATTAGGGACAGTTTTTGAAGAT
TAACTATAAAGGTGATAAAA

Downstream 100 bases:

>100_bases
TTTTTTATTTTTAGAGGGTGGTATTATAGAAAAAGAAATTATTTTTAATGAGGATTTTTATGATAAATTTATAAAATGCT
TGAAAGATAAGATGAAATTT

Product: thymidylate synthase

Products: dihydrofolate; dTMP

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MYCIKKPSVRASYEELVKEVLKNGEDMITEDGQKCREIRNTVIEITNPKLKSISPKYPLGKKAVEQYTNNLLYGSENVFS
YDYHSRLFKYPVNNKEINQIDFIVEKLKEQQNSRRCVAITWQPNIDIEVSNKDHGSVPCLQFVQFLIRDNKLYQTVLFRS
NDLAVAFVSNALGLIALGEMVAEKVGVDYGTYTHHSVSMHIYIDRDRDYIEKYFPESLKYLE

Sequences:

>Translated_222_residues
MYCIKKPSVRASYEELVKEVLKNGEDMITEDGQKCREIRNTVIEITNPKLKSISPKYPLGKKAVEQYTNNLLYGSENVFS
YDYHSRLFKYPVNNKEINQIDFIVEKLKEQQNSRRCVAITWQPNIDIEVSNKDHGSVPCLQFVQFLIRDNKLYQTVLFRS
NDLAVAFVSNALGLIALGEMVAEKVGVDYGTYTHHSVSMHIYIDRDRDYIEKYFPESLKYLE
>Mature_222_residues
MYCIKKPSVRASYEELVKEVLKNGEDMITEDGQKCREIRNTVIEITNPKLKSISPKYPLGKKAVEQYTNNLLYGSENVFS
YDYHSRLFKYPVNNKEINQIDFIVEKLKEQQNSRRCVAITWQPNIDIEVSNKDHGSVPCLQFVQFLIRDNKLYQTVLFRS
NDLAVAFVSNALGLIALGEMVAEKVGVDYGTYTHHSVSMHIYIDRDRDYIEKYFPESLKYLE

Specific function: May catalyze the biosynthesis of dTMP using an unknown cosubstrate [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Archaeal- type thyA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR014620
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: 2.1.1.45

Molecular weight: Translated: 25838; Mature: 25838

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYCIKKPSVRASYEELVKEVLKNGEDMITEDGQKCREIRNTVIEITNPKLKSISPKYPLG
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHEEECCCHHHCCCCCCCCC
KKAVEQYTNNLLYGSENVFSYDYHSRLFKYPVNNKEINQIDFIVEKLKEQQNSRRCVAIT
HHHHHHHHCCCCCCCCCEEEEHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
WQPNIDIEVSNKDHGSVPCLQFVQFLIRDNKLYQTVLFRSNDLAVAFVSNALGLIALGEM
ECCCEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHEECCCCEEEEEHHHHHHHHHHHHH
VAEKVGVDYGTYTHHSVSMHIYIDRDRDYIEKYFPESLKYLE
HHHHHCCCCCCCEEEEEEEEEEEECCHHHHHHHCHHHHHCCC
>Mature Secondary Structure
MYCIKKPSVRASYEELVKEVLKNGEDMITEDGQKCREIRNTVIEITNPKLKSISPKYPLG
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHEEECCCHHHCCCCCCCCC
KKAVEQYTNNLLYGSENVFSYDYHSRLFKYPVNNKEINQIDFIVEKLKEQQNSRRCVAIT
HHHHHHHHCCCCCCCCCEEEEHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
WQPNIDIEVSNKDHGSVPCLQFVQFLIRDNKLYQTVLFRSNDLAVAFVSNALGLIALGEM
ECCCEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHEECCCCEEEEEHHHHHHHHHHHHH
VAEKVGVDYGTYTHHSVSMHIYIDRDRDYIEKYFPESLKYLE
HHHHHCCCCCCCEEEEEEEEEEEECCHHHHHHHCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Dithiothreitol [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,10-methylenetetrahydrofolate; dUMP

Specific reaction: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP

General reaction: Methyl group transfer [C]

Inhibitor: 5'- monophosphate; 10-Formyl dihydrofolyl heptaglutamate; 10-Formyl dihydrofolyl monoglutamate; 10-Formyl tetrahydrofolyl heptaglutamate; 10-Formyl tetrahydrofolyl monoglutamate; Amethopterin; Dithiothreitol [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]