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The map label for this gene is hycG [H]

Identifier: 150401363

GI number: 150401363

Start: 960223

End: 960963

Strand: Reverse

Name: hycG [H]

Synonym: Maeo_0938

Alternate gene names: 150401363

Gene position: 960963-960223 (Counterclockwise)

Preceding gene: 150401364

Following gene: 150401359

Centisome position: 61.23

GC content: 36.44

Gene sequence:

>741_bases
ATGAACAACATAATTATCAACCGGATAACGCAGGGATATAGGACAGTTGAATTTCCCAGTGATAAAATACAGCTTCCCGA
AGCATACCGAGGAAGACCAGTCTTAAATCAGGATGCAGCAGATGAGGATGTGCAAAAAGTGGCATCTATATGCCCCACAG
GTGCAGTAAATCTTAATGATAGAACTTTGGACTTGGGAAAGTGCCTATTCTGTGGCAAATGTCAGTTTATAAAACAGAAT
TTTATAATATTTACAAGAGATTACAGAATGTTTGCCTTAAAAAGGGAAGATTTAATTATAAAAGATACATTAAAAGAACC
GGATACAAGTAGTTTAAAAAGTATAAAAAAATTATTTGGAAAATCTGTATATTTAAGAGAAGTATGTGCAGGTGGATGCA
ATGCCTGCGATGCCGATATAAATGTTTTGAATACGCCAGTTTATGACTTATCTAGGTTCGGTATTAAATTTGTGGCATCG
CCGAGACATGCAGATGGTCTTGTAATCACAGGACCAGTCACAAAAAATATGGAGCTCGCACTCATGAAAACATATGATGC
GACTCCTTCTCCAAAATTTGTTATAGCAGTTGGCACATGTGCAATATCGGGAGGAATATTTAGGGAAAACGAGGAAGTAG
TTGGCGGAGTTGATAAAATATTGCCTGTGGATTTATATATTCCCGGCTGCCCTCCACACCCATTTACAAATTTATATGCA
TTTGTAAAATTCTTTAAATAA

Upstream 100 bases:

>100_bases
TGAATTGGTAGTTAGAAATGAAGGAATATATGATTTCCCTCTGTGCAACAAAAGTTTTAACTTATCATACTGTGGTTTTG
ACCTTTAATGAGGTGTATAT

Downstream 100 bases:

>100_bases
TATCTTTAAAATACTTTTAATTCTTAAACTTTTTTAAACCACTTTCTTAATAGCATTCCATACCAAAAATGACTGTGGTT
TTAATGTTCCTTTTTGTGGA

Product: NADH ubiquinone oxidoreductase 20 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MNNIIINRITQGYRTVEFPSDKIQLPEAYRGRPVLNQDAADEDVQKVASICPTGAVNLNDRTLDLGKCLFCGKCQFIKQN
FIIFTRDYRMFALKREDLIIKDTLKEPDTSSLKSIKKLFGKSVYLREVCAGGCNACDADINVLNTPVYDLSRFGIKFVAS
PRHADGLVITGPVTKNMELALMKTYDATPSPKFVIAVGTCAISGGIFRENEEVVGGVDKILPVDLYIPGCPPHPFTNLYA
FVKFFK

Sequences:

>Translated_246_residues
MNNIIINRITQGYRTVEFPSDKIQLPEAYRGRPVLNQDAADEDVQKVASICPTGAVNLNDRTLDLGKCLFCGKCQFIKQN
FIIFTRDYRMFALKREDLIIKDTLKEPDTSSLKSIKKLFGKSVYLREVCAGGCNACDADINVLNTPVYDLSRFGIKFVAS
PRHADGLVITGPVTKNMELALMKTYDATPSPKFVIAVGTCAISGGIFRENEEVVGGVDKILPVDLYIPGCPPHPFTNLYA
FVKFFK
>Mature_246_residues
MNNIIINRITQGYRTVEFPSDKIQLPEAYRGRPVLNQDAADEDVQKVASICPTGAVNLNDRTLDLGKCLFCGKCQFIKQN
FIIFTRDYRMFALKREDLIIKDTLKEPDTSSLKSIKKLFGKSVYLREVCAGGCNACDADINVLNTPVYDLSRFGIKFVAS
PRHADGLVITGPVTKNMELALMKTYDATPSPKFVIAVGTCAISGGIFRENEEVVGGVDKILPVDLYIPGCPPHPFTNLYA
FVKFFK

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=113, Percent_Identity=41.5929203539823, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1789074, Length=140, Percent_Identity=46.4285714285714, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1788834, Length=139, Percent_Identity=42.4460431654676, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1788624, Length=112, Percent_Identity=41.9642857142857, Blast_Score=92, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=115, Percent_Identity=40, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI18859983, Length=115, Percent_Identity=40.8695652173913, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24642371, Length=115, Percent_Identity=40.8695652173913, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24651058, Length=115, Percent_Identity=40.8695652173913, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 27328; Mature: 27328

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIIINRITQGYRTVEFPSDKIQLPEAYRGRPVLNQDAADEDVQKVASICPTGAVNLND
CCCEEEEEECCCEEEEECCCCCEECCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
RTLDLGKCLFCGKCQFIKQNFIIFTRDYRMFALKREDLIIKDTLKEPDTSSLKSIKKLFG
CEECCCCEEEECCHHEEECCEEEEEECCEEEEEECCCEEEECCCCCCCHHHHHHHHHHHC
KSVYLREVCAGGCNACDADINVLNTPVYDLSRFGIKFVASPRHADGLVITGPVTKNMELA
CCHHHHHHHCCCCCCCCCCCEEECCCHHHHHHCCEEEEECCCCCCCEEEECCCCCCEEEE
LMKTYDATPSPKFVIAVGTCAISGGIFRENEEVVGGVDKILPVDLYIPGCPPHPFTNLYA
EEEECCCCCCCCEEEEEEEEEECCCEEECCCHHHCCHHHEEEEEEEECCCCCCCHHHHHH
FVKFFK
HHHHCC
>Mature Secondary Structure
MNNIIINRITQGYRTVEFPSDKIQLPEAYRGRPVLNQDAADEDVQKVASICPTGAVNLND
CCCEEEEEECCCEEEEECCCCCEECCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
RTLDLGKCLFCGKCQFIKQNFIIFTRDYRMFALKREDLIIKDTLKEPDTSSLKSIKKLFG
CEECCCCEEEECCHHEEECCEEEEEECCEEEEEECCCEEEECCCCCCCHHHHHHHHHHHC
KSVYLREVCAGGCNACDADINVLNTPVYDLSRFGIKFVASPRHADGLVITGPVTKNMELA
CCHHHHHHHCCCCCCCCCCCEEECCCHHHHHHCCEEEEECCCCCCCEEEECCCCCCEEEE
LMKTYDATPSPKFVIAVGTCAISGGIFRENEEVVGGVDKILPVDLYIPGCPPHPFTNLYA
EEEECCCCCCCCEEEEEEEEEECCCEEECCCHHHCCHHHEEEEEEEECCCCCCCHHHHHH
FVKFFK
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]