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The map label for this gene is rpoA2

Identifier: 150401217

GI number: 150401217

Start: 811441

End: 812634

Strand: Reverse

Name: rpoA2

Synonym: Maeo_0788

Alternate gene names: 150401217

Gene position: 812634-811441 (Counterclockwise)

Preceding gene: 150401218

Following gene: 150401216

Centisome position: 51.78

GC content: 33.5

Gene sequence:

>1194_bases
ATGGAAAAAACCATGCTGGAACAGAAATTAGAAAATACAATATTGCCATTATTGTTAAAAAAAGAATTAATAAATAAAAT
AATAAGTGAAAAAATAGAAGATGAAAATATAATACGAGATATAATTTCTGAAACTGTTAAATCATATGAAAGAACACTTG
TTGAACCTAATGAATCAGTTGGAGTTGTGGCAGCCCAATCCCTCGGGGAGCCCGGGACACAGATGACAATGCGAACATTT
CACTATGCGGGTGTTGCGGAGTTAAATGTTACGCTGGGTCTTCCAAGGATGATTGAGATTGTAGATGCTAGAAAAGAGCC
ATCTACTCCTACGATGACGGTATATTTAACAGAAGACTATGCATTTGATAAAAGTAAGGCAGAAGAAGTAGCAAAAAACA
TTGAAAGCATAACTGTTCAGAATATCGCACAAGATATACATATAGACATGGTTAAACTAGCTATAAATGTAGTATTAAAT
CCAGAATCAATGAAGCAACGAAATATAACTGCTGATGATGTGATGGAAGCTATTAAAAAGAAGATGAAATTAAAAATAGA
ACAGGAAGGCAATATTCTTCGATTAATAATAAAAACACCATCATTAAAAGCACTTAGAAAAAGAATTCCAAAAGTTAAAT
CCATTCATTTAAAGGGAGTTCCAAATATACCTCGGGTTATCGTTAAAAAAGATGATACAATGGGAGAATATATTCTACAC
AGTGAAGGTTCAAATTTAAGTGCCGTATTTGAGATTGATGGAGTAGATATGGTAAGAACTACTTCAAATAATATCGTGGA
GATTCAAGATGTATTGGGCATAGAAGCAGCTAGAAATGCCATTATAAATGAAATAAGTGGTGTTTTAAACCAGCAAGGTC
TTAATGTTGATATAAGGCATTTAATGGTGATAGCAGATATCATGACTGCTGACGGATCTGTTAAATCAATAGGTAGGCAT
GGATTAAGTGGTGAAAAAGCCTCAGTTCTTGCAAGAGCTGCATTTGAAGAAACTGTAAAACATTTATATTCTGCGGCTGA
AAAAGGACATTCCGATAAATTAAGCGGAGTTGTGGAAAATATCATTGTTGGAAAACCTATATCCATGGGAACTGGCTGTG
TGGATGTATATATAGATAGAGATTATGAAGAAGGAAAAGATTTGATGATAACGGTTGAAAAAGAGGAAAATTAA

Upstream 100 bases:

>100_bases
CCATATACATATATGCATATTTAATTATTTCATCTATATTATTTAATATTATTTTTTTAAATTTAAACATTTAAGATATA
CCCTTAAAATGGTGGAATAA

Downstream 100 bases:

>100_bases
TAATTAATGAAATATATAATTTTTATATTTGTTATTAATTTATTATTGTCATTAATCTACTACTATATTTTTTATTAATT
TATTTTATTATAGTAAATTA

Product: DNA-directed RNA polymerase subunit A''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MEKTMLEQKLENTILPLLLKKELINKIISEKIEDENIIRDIISETVKSYERTLVEPNESVGVVAAQSLGEPGTQMTMRTF
HYAGVAELNVTLGLPRMIEIVDARKEPSTPTMTVYLTEDYAFDKSKAEEVAKNIESITVQNIAQDIHIDMVKLAINVVLN
PESMKQRNITADDVMEAIKKKMKLKIEQEGNILRLIIKTPSLKALRKRIPKVKSIHLKGVPNIPRVIVKKDDTMGEYILH
SEGSNLSAVFEIDGVDMVRTTSNNIVEIQDVLGIEAARNAIINEISGVLNQQGLNVDIRHLMVIADIMTADGSVKSIGRH
GLSGEKASVLARAAFEETVKHLYSAAEKGHSDKLSGVVENIIVGKPISMGTGCVDVYIDRDYEEGKDLMITVEKEEN

Sequences:

>Translated_397_residues
MEKTMLEQKLENTILPLLLKKELINKIISEKIEDENIIRDIISETVKSYERTLVEPNESVGVVAAQSLGEPGTQMTMRTF
HYAGVAELNVTLGLPRMIEIVDARKEPSTPTMTVYLTEDYAFDKSKAEEVAKNIESITVQNIAQDIHIDMVKLAINVVLN
PESMKQRNITADDVMEAIKKKMKLKIEQEGNILRLIIKTPSLKALRKRIPKVKSIHLKGVPNIPRVIVKKDDTMGEYILH
SEGSNLSAVFEIDGVDMVRTTSNNIVEIQDVLGIEAARNAIINEISGVLNQQGLNVDIRHLMVIADIMTADGSVKSIGRH
GLSGEKASVLARAAFEETVKHLYSAAEKGHSDKLSGVVENIIVGKPISMGTGCVDVYIDRDYEEGKDLMITVEKEEN
>Mature_397_residues
MEKTMLEQKLENTILPLLLKKELINKIISEKIEDENIIRDIISETVKSYERTLVEPNESVGVVAAQSLGEPGTQMTMRTF
HYAGVAELNVTLGLPRMIEIVDARKEPSTPTMTVYLTEDYAFDKSKAEEVAKNIESITVQNIAQDIHIDMVKLAINVVLN
PESMKQRNITADDVMEAIKKKMKLKIEQEGNILRLIIKTPSLKALRKRIPKVKSIHLKGVPNIPRVIVKKDDTMGEYILH
SEGSNLSAVFEIDGVDMVRTTSNNIVEIQDVLGIEAARNAIINEISGVLNQQGLNVDIRHLMVIADIMTADGSVKSIGRH
GLSGEKASVLARAAFEETVKHLYSAAEKGHSDKLSGVVENIIVGKPISMGTGCVDVYIDRDYEEGKDLMITVEKEEN

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0086

COG function: function code K; DNA-directed RNA polymerase, beta' subunit/160 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta' chain family

Homologues:

Organism=Homo sapiens, GI39725938, Length=376, Percent_Identity=38.031914893617, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI4505939, Length=168, Percent_Identity=41.6666666666667, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI103471997, Length=102, Percent_Identity=43.1372549019608, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI25145495, Length=341, Percent_Identity=38.41642228739, Blast_Score=211, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI71987878, Length=436, Percent_Identity=31.4220183486239, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI71998295, Length=175, Percent_Identity=32, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320061, Length=433, Percent_Identity=33.256351039261, Blast_Score=213, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6324690, Length=374, Percent_Identity=37.1657754010695, Blast_Score=212, Evalue=9e-56,
Organism=Saccharomyces cerevisiae, GI6324917, Length=212, Percent_Identity=28.7735849056604, Blast_Score=93, Evalue=6e-20,
Organism=Drosophila melanogaster, GI281360912, Length=340, Percent_Identity=38.8235294117647, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI17530899, Length=452, Percent_Identity=30.5309734513274, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI17647875, Length=158, Percent_Identity=36.7088607594937, Blast_Score=98, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RPOA2_META3 (A6UV49)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324983.1
- ProteinModelPortal:   A6UV49
- SMR:   A6UV49
- STRING:   A6UV49
- GeneID:   5326917
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0788
- eggNOG:   arNOG04558
- HOGENOM:   HBG297327
- OMA:   LVADMMT
- ProtClustDB:   PRK04309
- BioCyc:   MAEO419665:MAEO_0788-MONOMER
- HAMAP:   MF_00411
- InterPro:   IPR007081
- InterPro:   IPR012757
- PANTHER:   PTHR19376:SF13
- TIGRFAMs:   TIGR02389

Pfam domain/function: PF04998 RNA_pol_Rpb1_5

EC number: =2.7.7.6

Molecular weight: Translated: 44052; Mature: 44052

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKTMLEQKLENTILPLLLKKELINKIISEKIEDENIIRDIISETVKSYERTLVEPNESV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GVVAAQSLGEPGTQMTMRTFHYAGVAELNVTLGLPRMIEIVDARKEPSTPTMTVYLTEDY
CEEEHHHCCCCCCHHHHHHHHHCCEEEEEEEECCHHHHHHHHCCCCCCCCEEEEEEECCC
AFDKSKAEEVAKNIESITVQNIAQDIHIDMVKLAINVVLNPESMKQRNITADDVMEAIKK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHCCCCHHHHHHHHHH
KMKLKIEQEGNILRLIIKTPSLKALRKRIPKVKSIHLKGVPNIPRVIVKKDDTMGEYILH
HHCEEEECCCCEEEEEEECCCHHHHHHHCCHHEEEEECCCCCCCEEEEECCCCCCCEEEE
SEGSNLSAVFEIDGVDMVRTTSNNIVEIQDVLGIEAARNAIINEISGVLNQQGLNVDIRH
CCCCCEEEEEEECCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LMVIADIMTADGSVKSIGRHGLSGEKASVLARAAFEETVKHLYSAAEKGHSDKLSGVVEN
HHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IIVGKPISMGTGCVDVYIDRDYEEGKDLMITVEKEEN
HHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCC
>Mature Secondary Structure
MEKTMLEQKLENTILPLLLKKELINKIISEKIEDENIIRDIISETVKSYERTLVEPNESV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GVVAAQSLGEPGTQMTMRTFHYAGVAELNVTLGLPRMIEIVDARKEPSTPTMTVYLTEDY
CEEEHHHCCCCCCHHHHHHHHHCCEEEEEEEECCHHHHHHHHCCCCCCCCEEEEEEECCC
AFDKSKAEEVAKNIESITVQNIAQDIHIDMVKLAINVVLNPESMKQRNITADDVMEAIKK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHCCCCHHHHHHHHHH
KMKLKIEQEGNILRLIIKTPSLKALRKRIPKVKSIHLKGVPNIPRVIVKKDDTMGEYILH
HHCEEEECCCCEEEEEEECCCHHHHHHHCCHHEEEEECCCCCCCEEEEECCCCCCCEEEE
SEGSNLSAVFEIDGVDMVRTTSNNIVEIQDVLGIEAARNAIINEISGVLNQQGLNVDIRH
CCCCCEEEEEEECCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LMVIADIMTADGSVKSIGRHGLSGEKASVLARAAFEETVKHLYSAAEKGHSDKLSGVVEN
HHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IIVGKPISMGTGCVDVYIDRDYEEGKDLMITVEKEEN
HHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA