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The map label for this gene is nadE

Identifier: 150401092

GI number: 150401092

Start: 680480

End: 681244

Strand: Reverse

Name: nadE

Synonym: Maeo_0662

Alternate gene names: 150401092

Gene position: 681244-680480 (Counterclockwise)

Preceding gene: 150401095

Following gene: 150401091

Centisome position: 43.41

GC content: 31.5

Gene sequence:

>765_bases
ATGAACTGCGAAGAATTAACAAATAAATTAACAAATTTTGTACTGTCAAAAGTAGGGGAAGCTAATGCAAAAGGTGTAGT
TTTAGGTTTAAGTGGTGGTATTGATAGCTCACTTGTGGCTACGATATGTGTAAAGGCATTAGGAAAGGATAATGTATTGG
GAGTAATAATGCCCGAAAAGAACTCAAATACACAGGATAAAGAACATGCTGAGCTTTTAGCAAATCAACTTGGAATAAAA
TATACTGTATCTGATATAACCGATGTTTTAAAATCTTTTGGTGCAGGGGGATATATTCCAACAAAAGAATTTGATAAAAT
GGCTGATGGAAATCTTAAACCAAGGATAAGAATGTGTATATTATATTATTTTGCAAATAAAAATAATCTTCTTGTGGCAG
GAACTTCAAATAAATCGGAAATATATATGGGATATGGGACAAAATACGGCGATTTGGGAAGTGATTTTTTATTAATCGGA
AACCTATTTAAAACAGAAGTTAGGGAGCTCTCTAAATATTTGGGTGTTCCAGATGAAATAATAAATAAAGCTCCATCAGC
TGGATTGTGGGAAGGTCAAACCGATGAAAAAGAATTGGGTATAACCTATGAATTGCTTGATAAGGTTCTAATGGCAATTG
AACAAAATAAAGAAAAAGAAGACATTTCAAAAGAATTAACTGTGCCAATTGAAAAAATAGAAGAAATATTAAATAGAATT
GAATCAAATAAGCATAAATCTCAGCCTATTCCTATTCCTAACTAA

Upstream 100 bases:

>100_bases
CAAAAATATTTATTTAATGTTAATTATATATTCTAACTGCGTATTAAATATATTATATATTATTATATTATCATAGTATC
ATATTAATATAGGTGGAACA

Downstream 100 bases:

>100_bases
TTTTAATTATAAATTTTATAGTTTATTATATTTTGTGGCACATTAGATTTTTTAGGGCTATATGGGACAAACTTTTAAAC
CTACCATAATATTTTTGGGA

Product: NAD+ synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MNCEELTNKLTNFVLSKVGEANAKGVVLGLSGGIDSSLVATICVKALGKDNVLGVIMPEKNSNTQDKEHAELLANQLGIK
YTVSDITDVLKSFGAGGYIPTKEFDKMADGNLKPRIRMCILYYFANKNNLLVAGTSNKSEIYMGYGTKYGDLGSDFLLIG
NLFKTEVRELSKYLGVPDEIINKAPSAGLWEGQTDEKELGITYELLDKVLMAIEQNKEKEDISKELTVPIEKIEEILNRI
ESNKHKSQPIPIPN

Sequences:

>Translated_254_residues
MNCEELTNKLTNFVLSKVGEANAKGVVLGLSGGIDSSLVATICVKALGKDNVLGVIMPEKNSNTQDKEHAELLANQLGIK
YTVSDITDVLKSFGAGGYIPTKEFDKMADGNLKPRIRMCILYYFANKNNLLVAGTSNKSEIYMGYGTKYGDLGSDFLLIG
NLFKTEVRELSKYLGVPDEIINKAPSAGLWEGQTDEKELGITYELLDKVLMAIEQNKEKEDISKELTVPIEKIEEILNRI
ESNKHKSQPIPIPN
>Mature_254_residues
MNCEELTNKLTNFVLSKVGEANAKGVVLGLSGGIDSSLVATICVKALGKDNVLGVIMPEKNSNTQDKEHAELLANQLGIK
YTVSDITDVLKSFGAGGYIPTKEFDKMADGNLKPRIRMCILYYFANKNNLLVAGTSNKSEIYMGYGTKYGDLGSDFLLIG
NLFKTEVRELSKYLGVPDEIINKAPSAGLWEGQTDEKELGITYELLDKVLMAIEQNKEKEDISKELTVPIEKIEEILNRI
ESNKHKSQPIPIPN

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=271, Percent_Identity=28.0442804428044, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_META3 (A6UUS4)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324858.1
- ProteinModelPortal:   A6UUS4
- SMR:   A6UUS4
- STRING:   A6UUS4
- GeneID:   5326908
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0662
- eggNOG:   arNOG08797
- HOGENOM:   HBG351567
- OMA:   FRYARDW
- BioCyc:   MAEO419665:MAEO_0662-MONOMER
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 27978; Mature: 27978

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: ACT_SITE 31-31

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNCEELTNKLTNFVLSKVGEANAKGVVLGLSGGIDSSLVATICVKALGKDNVLGVIMPEK
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECC
NSNTQDKEHAELLANQLGIKYTVSDITDVLKSFGAGGYIPTKEFDKMADGNLKPRIRMCI
CCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHEEE
LYYFANKNNLLVAGTSNKSEIYMGYGTKYGDLGSDFLLIGNLFKTEVRELSKYLGVPDEI
EEEEECCCCEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHH
INKAPSAGLWEGQTDEKELGITYELLDKVLMAIEQNKEKEDISKELTVPIEKIEEILNRI
HHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHH
ESNKHKSQPIPIPN
HCCCCCCCCCCCCC
>Mature Secondary Structure
MNCEELTNKLTNFVLSKVGEANAKGVVLGLSGGIDSSLVATICVKALGKDNVLGVIMPEK
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECC
NSNTQDKEHAELLANQLGIKYTVSDITDVLKSFGAGGYIPTKEFDKMADGNLKPRIRMCI
CCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHEEE
LYYFANKNNLLVAGTSNKSEIYMGYGTKYGDLGSDFLLIGNLFKTEVRELSKYLGVPDEI
EEEEECCCCEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHH
INKAPSAGLWEGQTDEKELGITYELLDKVLMAIEQNKEKEDISKELTVPIEKIEEILNRI
HHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHH
ESNKHKSQPIPIPN
HCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA