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The map label for this gene is nth [C]

Identifier: 150401058

GI number: 150401058

Start: 647498

End: 648571

Strand: Direct

Name: nth [C]

Synonym: Maeo_0628

Alternate gene names: 150401058

Gene position: 647498-648571 (Clockwise)

Preceding gene: 150401057

Following gene: 150401067

Centisome position: 41.26

GC content: 28.86

Gene sequence:

>1074_bases
ATGAAAACAACATATTTGGATATTTTAAAAGAAAAATTAAATAAAGATTCGGTGGTTGAGGAAATCTCAAAGAATAGTAA
AAATAGAAAAAATAAAGAAATTTTAGCTTTTAAGGTGCTTATATCCACAGTATTAAGTGCAAGAACTAAGGACGAAACCA
CAGATGAAGTTTCAAAAAGGCTATATAAAAAAGTTAAAAATATAGACGATTTAATAAATATTGATATTGAGGAGCTCCAA
GAATTAATTTATCCAGTTGGATTTTATAAAACCAAAGCAAAACATTTAAAAGAGTTAGCTTTGATGGTAAAAAACAATTA
CAACGGCAAAATACCAAATGATATTAATGAGCTGGTAAAACTTCCAGGAGTGGGGCGAAAAACTGCAAATTTAGTTATTA
CGCTGGCTTTTGACGACTATGGTATTTGTGTGGATACTCATGTCCATAGAATAAGCAACAGGTGGAATTTTGTAAATACT
CCCTCTCCTGAAAAAACTGAGATGGAGCTCCGTAAAAAATTGCCAAAAAAATATTGGAAAACCATCAATAATTCACTGGT
TGTATATGGTAGGGAGGTTTGTGCCCCAATTCCAAAATGTAGCAAATGTATTCAAGAAATAAAAGAAACCTGTCCTTATT
ATGATAAATTGATTTACTTTAATAGTTTGTTAAATAAATATAATTTTAGAAAAATATCCAAATCCAATATTCCAAATGAA
AAGGGAACCTATATTTTAAAAATAAAACTAAATGGTAGTAAAGTTATTGAATATGGAAAAACTAAAAAAGAAAAATTCCA
ACGAGGATATTATTTTTATGTTGGTTCTGCCATGGGGAGCTCCTCAAACTTAAAAAATAGAATAGGGAGACATCTTAAAA
ATGAGAATGAGAAAAAACTCCACTGGCATATTGATTATTTGTTAAAATATGGCAATGTAAAAGAGATATTTATTACAAAT
AATGCCGTTGAATGCGAAGTTTCAAAAGATTTAAAGGAAAAATTTGAATTTATAAAGGGATTTGGTTGTTCTGACTGTGG
TTGTGATAGTCATTTATATTATTTAAAACCGTGA

Upstream 100 bases:

>100_bases
GCTATGGGAATATTGAAATAAAATATTATTTTTAATAAATTTTTTTTATATTCTATTTTTTTATATTTATTAATTTAATT
TACACAATTTTAGGGAAATT

Downstream 100 bases:

>100_bases
ATATTATTGGTATTATTGTTAATATTTATAATTTTACAGTTCTTGAAGCACTTCTTCCAGCCCAACACTGAATACATATA
CCAACAGGAAGCGAAGCCGT

Product: DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKTTYLDILKEKLNKDSVVEEISKNSKNRKNKEILAFKVLISTVLSARTKDETTDEVSKRLYKKVKNIDDLINIDIEELQ
ELIYPVGFYKTKAKHLKELALMVKNNYNGKIPNDINELVKLPGVGRKTANLVITLAFDDYGICVDTHVHRISNRWNFVNT
PSPEKTEMELRKKLPKKYWKTINNSLVVYGREVCAPIPKCSKCIQEIKETCPYYDKLIYFNSLLNKYNFRKISKSNIPNE
KGTYILKIKLNGSKVIEYGKTKKEKFQRGYYFYVGSAMGSSSNLKNRIGRHLKNENEKKLHWHIDYLLKYGNVKEIFITN
NAVECEVSKDLKEKFEFIKGFGCSDCGCDSHLYYLKP

Sequences:

>Translated_357_residues
MKTTYLDILKEKLNKDSVVEEISKNSKNRKNKEILAFKVLISTVLSARTKDETTDEVSKRLYKKVKNIDDLINIDIEELQ
ELIYPVGFYKTKAKHLKELALMVKNNYNGKIPNDINELVKLPGVGRKTANLVITLAFDDYGICVDTHVHRISNRWNFVNT
PSPEKTEMELRKKLPKKYWKTINNSLVVYGREVCAPIPKCSKCIQEIKETCPYYDKLIYFNSLLNKYNFRKISKSNIPNE
KGTYILKIKLNGSKVIEYGKTKKEKFQRGYYFYVGSAMGSSSNLKNRIGRHLKNENEKKLHWHIDYLLKYGNVKEIFITN
NAVECEVSKDLKEKFEFIKGFGCSDCGCDSHLYYLKP
>Mature_357_residues
MKTTYLDILKEKLNKDSVVEEISKNSKNRKNKEILAFKVLISTVLSARTKDETTDEVSKRLYKKVKNIDDLINIDIEELQ
ELIYPVGFYKTKAKHLKELALMVKNNYNGKIPNDINELVKLPGVGRKTANLVITLAFDDYGICVDTHVHRISNRWNFVNT
PSPEKTEMELRKKLPKKYWKTINNSLVVYGREVCAPIPKCSKCIQEIKETCPYYDKLIYFNSLLNKYNFRKISKSNIPNE
KGTYILKIKLNGSKVIEYGKTKKEKFQRGYYFYVGSAMGSSSNLKNRIGRHLKNENEKKLHWHIDYLLKYGNVKEIFITN
NAVECEVSKDLKEKFEFIKGFGCSDCGCDSHLYYLKP

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=185, Percent_Identity=40.5405405405405, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1787920, Length=182, Percent_Identity=32.967032967033, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789331, Length=217, Percent_Identity=26.2672811059908, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17554540, Length=178, Percent_Identity=42.6966292134831, Blast_Score=154, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6324530, Length=182, Percent_Identity=35.1648351648352, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6319304, Length=168, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=38.8888888888889, Blast_Score=135, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR002837
- InterPro:   IPR004036
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR000305 [H]

Pfam domain/function: PF01986 DUF123; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 4.2.99.18

Molecular weight: Translated: 41536; Mature: 41536

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTYLDILKEKLNKDSVVEEISKNSKNRKNKEILAFKVLISTVLSARTKDETTDEVSKR
CCCHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LYKKVKNIDDLINIDIEELQELIYPVGFYKTKAKHLKELALMVKNNYNGKIPNDINELVK
HHHHHHCHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LPGVGRKTANLVITLAFDDYGICVDTHVHRISNRWNFVNTPSPEKTEMELRKKLPKKYWK
CCCCCCCCCEEEEEEEECCCCEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TINNSLVVYGREVCAPIPKCSKCIQEIKETCPYYDKLIYFNSLLNKYNFRKISKSNIPNE
HHCCCEEEECHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCCCCC
KGTYILKIKLNGSKVIEYGKTKKEKFQRGYYFYVGSAMGSSSNLKNRIGRHLKNENEKKL
CCCEEEEEEECCCHHHHCCCCHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCEE
HWHIDYLLKYGNVKEIFITNNAVECEVSKDLKEKFEFIKGFGCSDCGCDSHLYYLKP
EEEEEHHHHCCCEEEEEEECCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MKTTYLDILKEKLNKDSVVEEISKNSKNRKNKEILAFKVLISTVLSARTKDETTDEVSKR
CCCHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
LYKKVKNIDDLINIDIEELQELIYPVGFYKTKAKHLKELALMVKNNYNGKIPNDINELVK
HHHHHHCHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LPGVGRKTANLVITLAFDDYGICVDTHVHRISNRWNFVNTPSPEKTEMELRKKLPKKYWK
CCCCCCCCCEEEEEEEECCCCEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
TINNSLVVYGREVCAPIPKCSKCIQEIKETCPYYDKLIYFNSLLNKYNFRKISKSNIPNE
HHCCCEEEECHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCCCCC
KGTYILKIKLNGSKVIEYGKTKKEKFQRGYYFYVGSAMGSSSNLKNRIGRHLKNENEKKL
CCCEEEEEEECCCHHHHCCCCHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCEE
HWHIDYLLKYGNVKEIFITNNAVECEVSKDLKEKFEFIKGFGCSDCGCDSHLYYLKP
EEEEEHHHHCCCEEEEEEECCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Carbon-Oxygen Lyase [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]