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The map label for this gene is trm1

Identifier: 150401054

GI number: 150401054

Start: 643078

End: 644205

Strand: Direct

Name: trm1

Synonym: Maeo_0624

Alternate gene names: 150401054

Gene position: 643078-644205 (Clockwise)

Preceding gene: 150401047

Following gene: 150401057

Centisome position: 40.97

GC content: 31.03

Gene sequence:

>1128_bases
ATGAAATACATCACAGAAGGAAACACCAAATTAAAAATACCCGAAGATGCCACAATATCAAAAAAGGATAAAATATTTTA
TAATCCAATAATGGAAGTAAATAGGGACATATCAGTTTCAATAGTACAAAGTTTTTTAAATAAGTACGATAGAGATGAAT
TTTTTATTTGTGACCCTTTGGGGGGAAGTGGAGCTCGGGGATTAAGGTATGCCAATGAATTAAAAATAAATTCAGGAACA
GGAGCTCCTCACATCACCATAGGAGACATAAACCCAAATGCCATATCGTTAGCACAAGAAAATGCAAAATTAAATAATTT
AAACAATGTAAATATAGTTCATAAAGATGCAAATGTGCTTCTTTCGGAGAATTTTAGAAAATTTAATGTTGTAGATATTG
ACCCATTCGGAGCTCCCTCCCCATATTTAGATAGTGCTATAAGAAGTATTATAACCAAAAATGGTATATTATGTATGACT
GCCACAGATACGGCTGTGCTTTACGGCTCATATAGAAAAACATGTATAAGAAATTACGATGCCACACCATTAAAAGGAAA
TAAAGAACTTGCCATTAGGTTGTTGGTGGGACATGCCATAAGAATGGCATCAAAATACGATATTGGTCTTAAACCAATTT
TTTCACATTTCACGGCACATTATGTTAGAACTTTTTTAATGACTGAAAGAGGAGCTAAAAAAGCCGATGAAGCCGTTGAT
AAATTAGGATATGTTAAAAATATAGAGGAAGAATATATTATTAAATCCAGAAAAGAAGGCAATAGTGATGGTTTCTGTGG
ATTGTATTATTTGGATAATTTAACAGATGAAAATATAACAAAAGATGCCATAAAAATTGCAGAAGAAAGAAATTACTCAA
AAGAAAGCGTTAAAGTAATGAAAACCGTATATGAGGAAAGTTTAATAAATAATGTTGGTTGTTATGATGTTCATAAAATA
TGTAAAAACATAAAAAAGAAAGTGCCTCCTGTCGACATATTAATGAGTGGATTAAATGAAATGGGCTTTAACGCGTGTAG
AACTCACCATAACCCACATAGTATAAAATCTGATGCAAAATTAATCGATATTGTTGAATTTATTTATAAATACGATGGAA
ATAAATGA

Upstream 100 bases:

>100_bases
AATAATAAGAATAATAATAGAGAATAATAAGAGAGTAATAATAAGAACAATAATAAAAATAAAATAATAATTCATTAACT
AACTAAAACACGGTGAATTT

Downstream 100 bases:

>100_bases
AACTATGAACCTGGAATAATTACATTAAATAGCAAATTCCGGTATAATGATTGAATTGCAAAAGAAACCATGGAAATATT
GAAAAAGAAATAAAAATAAT

Product: N(2),N(2)-dimethylguanosine tRNA methyltransferase

Products: NA

Alternate protein names: tRNA 2,2-dimethylguanosine-26 methyltransferase; tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA(m(2,2)G26)dimethyltransferase

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKYITEGNTKLKIPEDATISKKDKIFYNPIMEVNRDISVSIVQSFLNKYDRDEFFICDPLGGSGARGLRYANELKINSGT
GAPHITIGDINPNAISLAQENAKLNNLNNVNIVHKDANVLLSENFRKFNVVDIDPFGAPSPYLDSAIRSIITKNGILCMT
ATDTAVLYGSYRKTCIRNYDATPLKGNKELAIRLLVGHAIRMASKYDIGLKPIFSHFTAHYVRTFLMTERGAKKADEAVD
KLGYVKNIEEEYIIKSRKEGNSDGFCGLYYLDNLTDENITKDAIKIAEERNYSKESVKVMKTVYEESLINNVGCYDVHKI
CKNIKKKVPPVDILMSGLNEMGFNACRTHHNPHSIKSDAKLIDIVEFIYKYDGNK

Sequences:

>Translated_375_residues
MKYITEGNTKLKIPEDATISKKDKIFYNPIMEVNRDISVSIVQSFLNKYDRDEFFICDPLGGSGARGLRYANELKINSGT
GAPHITIGDINPNAISLAQENAKLNNLNNVNIVHKDANVLLSENFRKFNVVDIDPFGAPSPYLDSAIRSIITKNGILCMT
ATDTAVLYGSYRKTCIRNYDATPLKGNKELAIRLLVGHAIRMASKYDIGLKPIFSHFTAHYVRTFLMTERGAKKADEAVD
KLGYVKNIEEEYIIKSRKEGNSDGFCGLYYLDNLTDENITKDAIKIAEERNYSKESVKVMKTVYEESLINNVGCYDVHKI
CKNIKKKVPPVDILMSGLNEMGFNACRTHHNPHSIKSDAKLIDIVEFIYKYDGNK
>Mature_375_residues
MKYITEGNTKLKIPEDATISKKDKIFYNPIMEVNRDISVSIVQSFLNKYDRDEFFICDPLGGSGARGLRYANELKINSGT
GAPHITIGDINPNAISLAQENAKLNNLNNVNIVHKDANVLLSENFRKFNVVDIDPFGAPSPYLDSAIRSIITKNGILCMT
ATDTAVLYGSYRKTCIRNYDATPLKGNKELAIRLLVGHAIRMASKYDIGLKPIFSHFTAHYVRTFLMTERGAKKADEAVD
KLGYVKNIEEEYIIKSRKEGNSDGFCGLYYLDNLTDENITKDAIKIAEERNYSKESVKVMKTVYEESLINNVGCYDVHKI
CKNIKKKVPPVDILMSGLNEMGFNACRTHHNPHSIKSDAKLIDIVEFIYKYDGNK

Specific function: Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups

COG id: COG1867

COG function: function code J; N2,N2-dimethylguanosine tRNA methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TRM1 family

Homologues:

Organism=Homo sapiens, GI216548445, Length=323, Percent_Identity=25.6965944272446, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI163792194, Length=286, Percent_Identity=29.7202797202797, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI8923219, Length=351, Percent_Identity=24.5014245014245, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI209862871, Length=351, Percent_Identity=24.5014245014245, Blast_Score=97, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17564890, Length=406, Percent_Identity=26.6009852216749, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320325, Length=469, Percent_Identity=24.0938166311301, Blast_Score=116, Evalue=5e-27,
Organism=Drosophila melanogaster, GI19921190, Length=275, Percent_Identity=24.7272727272727, Blast_Score=98, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRM1_META3 (A6UUN6)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324820.1
- STRING:   A6UUN6
- GeneID:   5327373
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0624
- eggNOG:   arNOG04822
- HOGENOM:   HBG577872
- OMA:   FYNADQK
- ProtClustDB:   PRK04338
- BioCyc:   MAEO419665:MAEO_0624-MONOMER
- HAMAP:   MF_00290
- InterPro:   IPR022923
- InterPro:   IPR002905
- PANTHER:   PTHR10631
- TIGRFAMs:   TIGR00308

Pfam domain/function: PF02005 TRM

EC number: =2.1.1.32

Molecular weight: Translated: 42202; Mature: 42202

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYITEGNTKLKIPEDATISKKDKIFYNPIMEVNRDISVSIVQSFLNKYDRDEFFICDPL
CCCEECCCCEEECCCCCCCCCCCCEEECCHHHHCCCCHHHHHHHHHHHCCCCCEEEECCC
GGSGARGLRYANELKINSGTGAPHITIGDINPNAISLAQENAKLNNLNNVNIVHKDANVL
CCCCCCCCEEHHEEEEECCCCCCEEEEECCCCCEEEHHHHCCCCCCCCCCEEEECCCCCC
LSENFRKFNVVDIDPFGAPSPYLDSAIRSIITKNGILCMTATDTAVLYGSYRKTCIRNYD
EECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEHHHHHHHHHCCC
ATPLKGNKELAIRLLVGHAIRMASKYDIGLKPIFSHFTAHYVRTFLMTERGAKKADEAVD
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KLGYVKNIEEEYIIKSRKEGNSDGFCGLYYLDNLTDENITKDAIKIAEERNYSKESVKVM
HHHHHHCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHH
KTVYEESLINNVGCYDVHKICKNIKKKVPPVDILMSGLNEMGFNACRTHHNPHSIKSDAK
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCHH
LIDIVEFIYKYDGNK
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MKYITEGNTKLKIPEDATISKKDKIFYNPIMEVNRDISVSIVQSFLNKYDRDEFFICDPL
CCCEECCCCEEECCCCCCCCCCCCEEECCHHHHCCCCHHHHHHHHHHHCCCCCEEEECCC
GGSGARGLRYANELKINSGTGAPHITIGDINPNAISLAQENAKLNNLNNVNIVHKDANVL
CCCCCCCCEEHHEEEEECCCCCCEEEEECCCCCEEEHHHHCCCCCCCCCCEEEECCCCCC
LSENFRKFNVVDIDPFGAPSPYLDSAIRSIITKNGILCMTATDTAVLYGSYRKTCIRNYD
EECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEHHHHHHHHHCCC
ATPLKGNKELAIRLLVGHAIRMASKYDIGLKPIFSHFTAHYVRTFLMTERGAKKADEAVD
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KLGYVKNIEEEYIIKSRKEGNSDGFCGLYYLDNLTDENITKDAIKIAEERNYSKESVKVM
HHHHHHCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHH
KTVYEESLINNVGCYDVHKICKNIKKKVPPVDILMSGLNEMGFNACRTHHNPHSIKSDAK
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCHH
LIDIVEFIYKYDGNK
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA