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The map label for this gene is rlmE

Identifier: 150401023

GI number: 150401023

Start: 608389

End: 609198

Strand: Direct

Name: rlmE

Synonym: Maeo_0592

Alternate gene names: 150401023

Gene position: 608389-609198 (Clockwise)

Preceding gene: 150401018

Following gene: 150401027

Centisome position: 38.76

GC content: 31.36

Gene sequence:

>810_bases
ATGGGAAAAAAAGATAAACGATGGGTAATGCAAAGAAAAAACGATTTTTACTACAAACTTGCAAAACAAAATCATTACCG
TTCAAGAGCATCATATAAATTACTTCAATTAAATGAAAAATTTGAAATAATTTACGAAGGAGATAATGTTGTGGATTTAG
GTTGTGCCCCCGGTGGTTGGTTGCAAGCCTCCTATGACCTTGTGGGGGAAGATGGCTTTGTAATTGGTGTGGATTTACAA
AAAGTAAAGCCATTAAGGGAAGAAAATATTGTAGCTATTCAGGGCGATATGACTAAAAAAGAAACAATAAATAAAATAAT
AGATTTAATGCCCTCTAAGGCTGATGTTGTAATATGCGATGCTTCACCGAATATTAGTGGAGTTTGGGAAGTTGACCATT
CTCGTTCTATTGAATTATCTTTAATGGCACTAATTGCTACAACTCATTTATTAAGAAAAAACGGTAATTTTGTGGTAAAA
GTATTCCAAGGTAGTTTATTTGACCAGTATGTCCAATTATTAACAAAATACTTTAAAAAAGTCCAAACCACAAAACCTAA
AGCCTCCCGTTCTGTTAGTGCCGAAGTTTATGTAATTGGAAAAAAATTTTTAGGAAAAAAATTTGATAAAAATACAGATT
TTCCGATTATTAAATTAATAGAGGATAATGAATTTTTAGATAGGTATATTGAAGAAACACATGAAAAAAAAGAGGGCAAT
GAAACTTCTGACAATGATGAGGACAATAACAAAAACGGGCTTATGATTAAAAAAATCAAGGAGCTCCGAGGAAAAAGAAG
TAAATTATAA

Upstream 100 bases:

>100_bases
ATTCTTATTATTTTTCATTATTAACAATATTACATAATGTTTTTATGATATATAATATTTTTATTTAATTTAAAAATTAA
TCAAAATTAACTGTGATACA

Downstream 100 bases:

>100_bases
TATTTAATTAATTTATTTCCAAAGTTCCAATTCTTTTCTTTTAATATCTTGCCTATGTCTTTCAAGGTATTTATACATTT
TAGAGTGAGAATTTCCCCTT

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MGKKDKRWVMQRKNDFYYKLAKQNHYRSRASYKLLQLNEKFEIIYEGDNVVDLGCAPGGWLQASYDLVGEDGFVIGVDLQ
KVKPLREENIVAIQGDMTKKETINKIIDLMPSKADVVICDASPNISGVWEVDHSRSIELSLMALIATTHLLRKNGNFVVK
VFQGSLFDQYVQLLTKYFKKVQTTKPKASRSVSAEVYVIGKKFLGKKFDKNTDFPIIKLIEDNEFLDRYIEETHEKKEGN
ETSDNDEDNNKNGLMIKKIKELRGKRSKL

Sequences:

>Translated_269_residues
MGKKDKRWVMQRKNDFYYKLAKQNHYRSRASYKLLQLNEKFEIIYEGDNVVDLGCAPGGWLQASYDLVGEDGFVIGVDLQ
KVKPLREENIVAIQGDMTKKETINKIIDLMPSKADVVICDASPNISGVWEVDHSRSIELSLMALIATTHLLRKNGNFVVK
VFQGSLFDQYVQLLTKYFKKVQTTKPKASRSVSAEVYVIGKKFLGKKFDKNTDFPIIKLIEDNEFLDRYIEETHEKKEGN
ETSDNDEDNNKNGLMIKKIKELRGKRSKL
>Mature_268_residues
GKKDKRWVMQRKNDFYYKLAKQNHYRSRASYKLLQLNEKFEIIYEGDNVVDLGCAPGGWLQASYDLVGEDGFVIGVDLQK
VKPLREENIVAIQGDMTKKETINKIIDLMPSKADVVICDASPNISGVWEVDHSRSIELSLMALIATTHLLRKNGNFVVKV
FQGSLFDQYVQLLTKYFKKVQTTKPKASRSVSAEVYVIGKKFLGKKFDKNTDFPIIKLIEDNEFLDRYIEETHEKKEGNE
TSDNDEDNNKNGLMIKKIKELRGKRSKL

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI194097365, Length=206, Percent_Identity=37.8640776699029, Blast_Score=153, Evalue=1e-37,
Organism=Homo sapiens, GI7019377, Length=209, Percent_Identity=36.3636363636364, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI29029591, Length=194, Percent_Identity=37.6288659793814, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI29029589, Length=194, Percent_Identity=37.6288659793814, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI7110661, Length=194, Percent_Identity=37.6288659793814, Blast_Score=138, Evalue=4e-33,
Organism=Escherichia coli, GI1789569, Length=195, Percent_Identity=36.4102564102564, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17553860, Length=200, Percent_Identity=42, Blast_Score=157, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI71987561, Length=200, Percent_Identity=42, Blast_Score=157, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI71987550, Length=200, Percent_Identity=42, Blast_Score=157, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17554650, Length=191, Percent_Identity=37.696335078534, Blast_Score=142, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17553474, Length=202, Percent_Identity=35.6435643564356, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319796, Length=209, Percent_Identity=37.799043062201, Blast_Score=141, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6319535, Length=210, Percent_Identity=34.7619047619048, Blast_Score=124, Evalue=1e-29,
Organism=Drosophila melanogaster, GI18859957, Length=206, Percent_Identity=38.8349514563107, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24647580, Length=215, Percent_Identity=35.3488372093023, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24648639, Length=201, Percent_Identity=33.3333333333333, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21356387, Length=212, Percent_Identity=26.4150943396226, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_META3 (A6UUK5)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324789.1
- ProteinModelPortal:   A6UUK5
- SMR:   A6UUK5
- STRING:   A6UUK5
- GeneID:   5327424
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0592
- eggNOG:   arNOG05244
- HOGENOM:   HBG398270
- OMA:   DASPNIS
- ProtClustDB:   CLSK876556
- BioCyc:   MAEO419665:MAEO_0592-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 30936; Mature: 30805

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: ACT_SITE 160-160 BINDING 58-58 BINDING 60-60 BINDING 78-78 BINDING 96-96 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKDKRWVMQRKNDFYYKLAKQNHYRSRASYKLLQLNEKFEIIYEGDNVVDLGCAPGGW
CCCCCHHHHHHCCCCEEEEEECCCCHHHHHCEEEEEECCEEEEEECCCCEEEECCCCCCC
LQASYDLVGEDGFVIGVDLQKVKPLREENIVAIQGDMTKKETINKIIDLMPSKADVVICD
EEEEEEEECCCCEEEEEEHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEC
ASPNISGVWEVDHSRSIELSLMALIATTHLLRKNGNFVVKVFQGSLFDQYVQLLTKYFKK
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
VQTTKPKASRSVSAEVYVIGKKFLGKKFDKNTDFPIIKLIEDNEFLDRYIEETHEKKEGN
HHCCCCCCCCCCCEEEEEEEHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCC
ETSDNDEDNNKNGLMIKKIKELRGKRSKL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GKKDKRWVMQRKNDFYYKLAKQNHYRSRASYKLLQLNEKFEIIYEGDNVVDLGCAPGGW
CCCCHHHHHHCCCCEEEEEECCCCHHHHHCEEEEEECCEEEEEECCCCEEEECCCCCCC
LQASYDLVGEDGFVIGVDLQKVKPLREENIVAIQGDMTKKETINKIIDLMPSKADVVICD
EEEEEEEECCCCEEEEEEHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCEEEEC
ASPNISGVWEVDHSRSIELSLMALIATTHLLRKNGNFVVKVFQGSLFDQYVQLLTKYFKK
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
VQTTKPKASRSVSAEVYVIGKKFLGKKFDKNTDFPIIKLIEDNEFLDRYIEETHEKKEGN
HHCCCCCCCCCCCEEEEEEEHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCC
ETSDNDEDNNKNGLMIKKIKELRGKRSKL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA