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The map label for this gene is mptN [H]

Identifier: 150400995

GI number: 150400995

Start: 581563

End: 582444

Strand: Direct

Name: mptN [H]

Synonym: Maeo_0564

Alternate gene names: 150400995

Gene position: 581563-582444 (Clockwise)

Preceding gene: 150400993

Following gene: 150400998

Centisome position: 37.05

GC content: 30.73

Gene sequence:

>882_bases
ATGAAAATAGGAATTATTACAGAAGAAAGGGACTGGATTACCTGTTCGTTGGAACGAGTTTTAATTAAAAGGGATATTAC
TCCGATATATATAAAACCTTCCAAAATAGTTTCATTTACTGGAATGGACATAAAATTTAAATGTAATAATGAAAATCTAT
TAAAATTAAATTGTGCTTTTGTTAGGGACCTCGGTGATGGGACTGATTATTATAGATTTGATGCCCTAAAATATTTGGGG
GAAGTTATGCCGTTGATAAATCCAGCAGAAGGACTGGAAAATGCAGGAAATAAATATAAAACTTCAATGATTTTGGATAA
AAACAACATACCACACCCTAAAACCATCATTACAGAAGATTTAGATAAAGCACTAATGTGGTCAGAAAAATTTAAAGATT
GTGTCGTAAAACCAATATTTGGCAATAGAGGGAAAGGCATTATTAGATTGGGAAATAAACCAATAATTAATAAAATAAAT
CTATTAAAAGAATTTAAGAAAAAATACGGCTTATTTTATATTCAGGAATTTATACGAAATCCAAATAATGTATATAGAGA
TATAAGGGCATTTGTTATTGGAGATAAGGTTGTTTCTGCAATGTATAGGACCTCAAATAATTGGCTAACCAACATACACC
AGCAGGGAACTGGAAAACCTTGCGAAATTACACCAGAAATCGAAGAATTAGCGATTAAAGCAAAAGATTCTATGGGGCTT
ATATATGGTGGTGTGGATTTGATGGAAAGTAAAGATGGGCTAAAAGTAATAGAAGTTAATGGAGCTCCCTCATGGGAAAC
TCTTTCGAAAGTTTCAAATGTAAATGTCACTGAAAAATTAATAGATTTCGTGTTGAATAATGTTAAAGATAGATATTATT
AA

Upstream 100 bases:

>100_bases
ATATGATATATATAGTAAATAAGTAATAATATATTGTATATAACATAGATATATTTAATGATATAAAAATTAAATTAATA
GTAGCTATAATCGTGAAAAA

Downstream 100 bases:

>100_bases
CAAACCCGTAAATATATTTCTCTGTTGTTGTATCGTAATAAAAGATATATTATATACACACCTTAACTATACGATACGGG
AAATTGTTCGAAGATTGATG

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: H(4)MPT:alpha-L-glutamate ligase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKIGIITEERDWITCSLERVLIKRDITPIYIKPSKIVSFTGMDIKFKCNNENLLKLNCAFVRDLGDGTDYYRFDALKYLG
EVMPLINPAEGLENAGNKYKTSMILDKNNIPHPKTIITEDLDKALMWSEKFKDCVVKPIFGNRGKGIIRLGNKPIINKIN
LLKEFKKKYGLFYIQEFIRNPNNVYRDIRAFVIGDKVVSAMYRTSNNWLTNIHQQGTGKPCEITPEIEELAIKAKDSMGL
IYGGVDLMESKDGLKVIEVNGAPSWETLSKVSNVNVTEKLIDFVLNNVKDRYY

Sequences:

>Translated_293_residues
MKIGIITEERDWITCSLERVLIKRDITPIYIKPSKIVSFTGMDIKFKCNNENLLKLNCAFVRDLGDGTDYYRFDALKYLG
EVMPLINPAEGLENAGNKYKTSMILDKNNIPHPKTIITEDLDKALMWSEKFKDCVVKPIFGNRGKGIIRLGNKPIINKIN
LLKEFKKKYGLFYIQEFIRNPNNVYRDIRAFVIGDKVVSAMYRTSNNWLTNIHQQGTGKPCEITPEIEELAIKAKDSMGL
IYGGVDLMESKDGLKVIEVNGAPSWETLSKVSNVNVTEKLIDFVLNNVKDRYY
>Mature_293_residues
MKIGIITEERDWITCSLERVLIKRDITPIYIKPSKIVSFTGMDIKFKCNNENLLKLNCAFVRDLGDGTDYYRFDALKYLG
EVMPLINPAEGLENAGNKYKTSMILDKNNIPHPKTIITEDLDKALMWSEKFKDCVVKPIFGNRGKGIIRLGNKPIINKIN
LLKEFKKKYGLFYIQEFIRNPNNVYRDIRAFVIGDKVVSAMYRTSNNWLTNIHQQGTGKPCEITPEIEELAIKAKDSMGL
IYGGVDLMESKDGLKVIEVNGAPSWETLSKVSNVNVTEKLIDFVLNNVKDRYY

Specific function: Catalyzes the ATP or GTP-dependent addition of one L- glutamate molecule to tetrahydromethanopterin, producing tetrahydrosarcinapterin [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1787076, Length=241, Percent_Identity=27.3858921161826, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 33515; Mature: 33515

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIITEERDWITCSLERVLIKRDITPIYIKPSKIVSFTGMDIKFKCNNENLLKLNCAF
CEEEEEECCCCEEEHHHHHHHHHCCCCEEEEECCCEEEEECCEEEEEECCCCEEEEEEEE
VRDLGDGTDYYRFDALKYLGEVMPLINPAEGLENAGNKYKTSMILDKNNIPHPKTIITED
EEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHH
LDKALMWSEKFKDCVVKPIFGNRGKGIIRLGNKPIINKINLLKEFKKKYGLFYIQEFIRN
HHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCHHHHHHHHHC
PNNVYRDIRAFVIGDKVVSAMYRTSNNWLTNIHQQGTGKPCEITPEIEELAIKAKDSMGL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCEECCCHHHHEEECCCCCEE
IYGGVDLMESKDGLKVIEVNGAPSWETLSKVSNVNVTEKLIDFVLNNVKDRYY
EEECCEEEECCCCCEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIGIITEERDWITCSLERVLIKRDITPIYIKPSKIVSFTGMDIKFKCNNENLLKLNCAF
CEEEEEECCCCEEEHHHHHHHHHCCCCEEEEECCCEEEEECCEEEEEECCCCEEEEEEEE
VRDLGDGTDYYRFDALKYLGEVMPLINPAEGLENAGNKYKTSMILDKNNIPHPKTIITED
EEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHH
LDKALMWSEKFKDCVVKPIFGNRGKGIIRLGNKPIINKINLLKEFKKKYGLFYIQEFIRN
HHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCHHHHHHHHHC
PNNVYRDIRAFVIGDKVVSAMYRTSNNWLTNIHQQGTGKPCEITPEIEELAIKAKDSMGL
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCEECCCHHHHEEECCCCCEE
IYGGVDLMESKDGLKVIEVNGAPSWETLSKVSNVNVTEKLIDFVLNNVKDRYY
EEECCEEEECCCCCEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA