Definition Methanococcus aeolicus Nankai-3, complete genome.
Accession NC_009635
Length 1,569,500

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The map label for this gene is purT

Identifier: 150400989

GI number: 150400989

Start: 573185

End: 574360

Strand: Direct

Name: purT

Synonym: Maeo_0558

Alternate gene names: 150400989

Gene position: 573185-574360 (Clockwise)

Preceding gene: 150400986

Following gene: 150400991

Centisome position: 36.52

GC content: 36.31

Gene sequence:

>1176_bases
ATGTTGTTAGGAACTCCTCTTTTTTCGAATGGAACAAAAATACTACTATTAGGTAGTGGTGAATTAGGAAAAGAAGTAAT
AATAGAAGCTCAGCGATTAGGTATAGAATGTGTTGCCGTTGATAGCTATGATAATGCCCCTGCAATGCAAGTTGCACATA
GAAGTTATGTAATAGATATGAAAGATGGAGATGCTTTAAAGGCAATTTTGGAAAAGGAAGTTCCAGACTATATTATACCA
GAAATAGAAGCCATAAATACAGATATACTCATAGAGATGGAAGAATTTGGACATCAAGTTGTACCTACGGCAAATGCCGC
AAAAATAACAATGGATAGGGAAGGAATAAGAAGATTAGCTTCCGAACAATTAAACATACCCACAGCAAAATATGAATTTG
CGGAATCGTTGGAAGAATTAAAAAAAGCTGTAAAAAAAATAGGAGCTCCCTGTATCGTAAAGCCAATAATGTCGTCATCT
GGAAAGGGACAAAGCACAGTTAAAAGTGAAAAAGACGAGGATATAGAATATGCATGGAATTATGCCCAAGCGGGAGCAAG
GGGCATAGGTAAAAAGGTAATAGTTGAAGAATTTATTAACTTTGATTATGAAATAACGCTGCTCACAGCAAGAACTGCTC
ACGGAACAGTGTATTGCCCACCAATTGGACACATTCAAGATGGGGGTGACTATATTGAAAGTTGGCAACCTCATCCTATG
AGTGAGGAAATGATAAAAAAAGCCCAGGAGATGGCAAATAAAATAACCACAGTATTGGGTGGAAACGGCATATTTGGTGT
AGAGTTATTTATTAGGGGGGACGAAGTAATATTTAGCGAAGTATCTCCGCGACCACATGATACTGGAATGGTTACAATGA
TTACCCAAAATATGAGTGAATTTGAAATACATTTAAGAAGTATATTGGGGCTTCCTGTGGATATTCAATTAATTACAGCA
GGTGCAAGTAGTGTTATTAAATCAAAAATACATAAATATGCACCACAATATGACATCTCCGATGCTGTGAAAGTTCCAAA
TACAAAATTAAGAATTTTCGGAAAACCACTCGCAAAAGTAGGTAGAAGAATGGGAGTTGCGTTGGCATCTGCAAATACAG
TTGAAGAAGCACGAAAAAATGCTAAAAAATGTGCCAATGGAGTAATTATTAGATAA

Upstream 100 bases:

>100_bases
TTATATTATATTAGTAATTACAATAGTATAATATCATTATTATATTATTCTATTTTTTAGTATTCTATTTATATTGTTAG
TATAATCAGGTGGTGAAAAT

Downstream 100 bases:

>100_bases
TATTGATATCAGCATGCATACATAAGGAATATAATATAAATAAAATGTAAAATATATTTTATAATATCTTTATTTTTTCA
TTTATTAAATAAAATACAGT

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MLLGTPLFSNGTKILLLGSGELGKEVIIEAQRLGIECVAVDSYDNAPAMQVAHRSYVIDMKDGDALKAILEKEVPDYIIP
EIEAINTDILIEMEEFGHQVVPTANAAKITMDREGIRRLASEQLNIPTAKYEFAESLEELKKAVKKIGAPCIVKPIMSSS
GKGQSTVKSEKDEDIEYAWNYAQAGARGIGKKVIVEEFINFDYEITLLTARTAHGTVYCPPIGHIQDGGDYIESWQPHPM
SEEMIKKAQEMANKITTVLGGNGIFGVELFIRGDEVIFSEVSPRPHDTGMVTMITQNMSEFEIHLRSILGLPVDIQLITA
GASSVIKSKIHKYAPQYDISDAVKVPNTKLRIFGKPLAKVGRRMGVALASANTVEEARKNAKKCANGVIIR

Sequences:

>Translated_391_residues
MLLGTPLFSNGTKILLLGSGELGKEVIIEAQRLGIECVAVDSYDNAPAMQVAHRSYVIDMKDGDALKAILEKEVPDYIIP
EIEAINTDILIEMEEFGHQVVPTANAAKITMDREGIRRLASEQLNIPTAKYEFAESLEELKKAVKKIGAPCIVKPIMSSS
GKGQSTVKSEKDEDIEYAWNYAQAGARGIGKKVIVEEFINFDYEITLLTARTAHGTVYCPPIGHIQDGGDYIESWQPHPM
SEEMIKKAQEMANKITTVLGGNGIFGVELFIRGDEVIFSEVSPRPHDTGMVTMITQNMSEFEIHLRSILGLPVDIQLITA
GASSVIKSKIHKYAPQYDISDAVKVPNTKLRIFGKPLAKVGRRMGVALASANTVEEARKNAKKCANGVIIR
>Mature_391_residues
MLLGTPLFSNGTKILLLGSGELGKEVIIEAQRLGIECVAVDSYDNAPAMQVAHRSYVIDMKDGDALKAILEKEVPDYIIP
EIEAINTDILIEMEEFGHQVVPTANAAKITMDREGIRRLASEQLNIPTAKYEFAESLEELKKAVKKIGAPCIVKPIMSSS
GKGQSTVKSEKDEDIEYAWNYAQAGARGIGKKVIVEEFINFDYEITLLTARTAHGTVYCPPIGHIQDGGDYIESWQPHPM
SEEMIKKAQEMANKITTVLGGNGIFGVELFIRGDEVIFSEVSPRPHDTGMVTMITQNMSEFEIHLRSILGLPVDIQLITA
GASSVIKSKIHKYAPQYDISDAVKVPNTKLRIFGKPLAKVGRRMGVALASANTVEEARKNAKKCANGVIIR

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=392, Percent_Identity=55.1020408163265, Blast_Score=430, Evalue=1e-122,
Organism=Escherichia coli, GI1786733, Length=359, Percent_Identity=25.0696378830084, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324702, Length=367, Percent_Identity=24.7956403269755, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_META3 (A6UUH1)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324755.1
- ProteinModelPortal:   A6UUH1
- SMR:   A6UUH1
- STRING:   A6UUH1
- GeneID:   5326491
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0558
- eggNOG:   arNOG04188
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- ProtClustDB:   PRK09288
- BioCyc:   MAEO419665:MAEO_0558-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42870; Mature: 42870

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 81-81 BINDING 113-113 BINDING 154-154 BINDING 204-204 BINDING 268-268 BINDING 280-280 BINDING 287-287 BINDING 355-355

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MLLGTPLFSNGTKILLLGSGELGKEVIIEAQRLGIECVAVDSYDNAPAMQVAHRSYVIDM
CEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCHHHEECCEEEEEC
KDGDALKAILEKEVPDYIIPEIEAINTDILIEMEEFGHQVVPTANAAKITMDREGIRRLA
CCCHHHHHHHHHCCCCCCCCCHHCCCCEEEEEHHHCCCEECCCCCCEEEEECHHHHHHHH
SEQLNIPTAKYEFAESLEELKKAVKKIGAPCIVKPIMSSSGKGQSTVKSEKDEDIEYAWN
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCCCCCCCHHCCCCCCCHHHHHH
YAQAGARGIGKKVIVEEFINFDYEITLLTARTAHGTVYCPPIGHIQDGGDYIESWQPHPM
HHHHHHHHCCHHHHHHHHHCCCEEEEEEEEECCCCEEECCCCCCCCCCCHHHHCCCCCCC
SEEMIKKAQEMANKITTVLGGNGIFGVELFIRGDEVIFSEVSPRPHDTGMVTMITQNMSE
CHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEEECCHHH
FEIHLRSILGLPVDIQLITAGASSVIKSKIHKYAPQYDISDAVKVPNTKLRIFGKPLAKV
HHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHH
GRRMGVALASANTVEEARKNAKKCANGVIIR
HHHHCCHHHCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLLGTPLFSNGTKILLLGSGELGKEVIIEAQRLGIECVAVDSYDNAPAMQVAHRSYVIDM
CEECCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCCHHHEECCEEEEEC
KDGDALKAILEKEVPDYIIPEIEAINTDILIEMEEFGHQVVPTANAAKITMDREGIRRLA
CCCHHHHHHHHHCCCCCCCCCHHCCCCEEEEEHHHCCCEECCCCCCEEEEECHHHHHHHH
SEQLNIPTAKYEFAESLEELKKAVKKIGAPCIVKPIMSSSGKGQSTVKSEKDEDIEYAWN
HHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEHHHHCCCCCCCCHHCCCCCCCHHHHHH
YAQAGARGIGKKVIVEEFINFDYEITLLTARTAHGTVYCPPIGHIQDGGDYIESWQPHPM
HHHHHHHHCCHHHHHHHHHCCCEEEEEEEEECCCCEEECCCCCCCCCCCHHHHCCCCCCC
SEEMIKKAQEMANKITTVLGGNGIFGVELFIRGDEVIFSEVSPRPHDTGMVTMITQNMSE
CHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEEECCHHH
FEIHLRSILGLPVDIQLITAGASSVIKSKIHKYAPQYDISDAVKVPNTKLRIFGKPLAKV
HHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHH
GRRMGVALASANTVEEARKNAKKCANGVIIR
HHHHCCHHHCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA