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The map label for this gene is carA [H]

Identifier: 150400898

GI number: 150400898

Start: 486905

End: 487978

Strand: Direct

Name: carA [H]

Synonym: Maeo_0466

Alternate gene names: 150400898

Gene position: 486905-487978 (Clockwise)

Preceding gene: 150400897

Following gene: 150400899

Centisome position: 31.02

GC content: 33.8

Gene sequence:

>1074_bases
ATGACGAAAGCCAAATTAATTTTAGAAGATGGAACTGTTATGCAGGGCAAAGGATTTGGTGCAGAAGGAGTTGCAGTTGG
AGAAATCATATTTTGCACAGGTATGACTGGATATGTGGAGGCATTAACCGACCCATCATATAAAGGTCAGATATTAACCA
TGACCTACCCGTTAATTGGAAACTATGGCGTAAATGAAGAATGGTTTGAATCTGATGGAATAAAAGTAGGCGGTTTTGTC
GTAAAAGAGAACTGTTTAACTCCCAGCCATCATAAATCTAAAAAAAATCTTGATGAATTTTTAAAAGAATATGATGTCTG
CGGAATATCCGACCTTGACACAAGAAAATTAACAAAAAAAATAAGGACTTATGGAGCCATGAAAGCAGTTATTGCAGTTG
GAGATATCGAAATAGATAATGAAGAATTATTAAAAAAAGTTAAATTGCACAAAAATATTTCAGAATTAAATTTAGTCTCC
GATGTATGCGTAAAAAATCCAATAATATTGGGAGGAGGTAAAAAGACTGTGGTTTTAATCGACTGTGGAGTTAAAAAATC
CATAGTAAATCAGCTAATTGATAAAAATATAAATTTGGTCATTGTCCCATACAATACAAGTTATAAAGATATATTGGAAT
ATAATCCAAATGGAGTGTTTATTTCAAATGGGCCCGGAGACCCTGCTGTCCTAAAAGAAACAATAAACACCGTGAAAAAC
CTTATCGGCAAAGTTCCAATATATGGAATATGTTTGGGGCATCAAATAATATCACTGGCACTTGGTGCTAAAACGAGAAA
ATTAAAATTTGGTCATCATGGATTAAACCAGCCTATTAAGGATTTGGAAAATGGAAGGGTATATATCACTTCCCAAAATC
ACAACTTTGCAGTTTCTGATTTGCCCGATTGTATGGACATTACACAGATTAATTTAAACGATAACAGCATTGAGGGAATA
AAACATAAGGAACTACCAATTGTCGGAGTTCAATATCACCCGGAAGCCAAACCTGGTCCTCATGATACATACGGATTCTT
TGACGATTTTAATAAGATTATTAAACAATATTAA

Upstream 100 bases:

>100_bases
TTTATTATTTACTACTAATTTTTAAGCTCAATACATTTACTCAGGTATTAGTCAATAATGACCATACACGGCACAATATA
ATATATAATAAAGGGATAGT

Downstream 100 bases:

>100_bases
TATATGTTAATATATAATATTAACTTACATTATTAATTTATATTATATAATTTGGTGGTTTTATGCCAAAAAGAGAAGAT
ATTAAAAAAATAATGATAAT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MTKAKLILEDGTVMQGKGFGAEGVAVGEIIFCTGMTGYVEALTDPSYKGQILTMTYPLIGNYGVNEEWFESDGIKVGGFV
VKENCLTPSHHKSKKNLDEFLKEYDVCGISDLDTRKLTKKIRTYGAMKAVIAVGDIEIDNEELLKKVKLHKNISELNLVS
DVCVKNPIILGGGKKTVVLIDCGVKKSIVNQLIDKNINLVIVPYNTSYKDILEYNPNGVFISNGPGDPAVLKETINTVKN
LIGKVPIYGICLGHQIISLALGAKTRKLKFGHHGLNQPIKDLENGRVYITSQNHNFAVSDLPDCMDITQINLNDNSIEGI
KHKELPIVGVQYHPEAKPGPHDTYGFFDDFNKIIKQY

Sequences:

>Translated_357_residues
MTKAKLILEDGTVMQGKGFGAEGVAVGEIIFCTGMTGYVEALTDPSYKGQILTMTYPLIGNYGVNEEWFESDGIKVGGFV
VKENCLTPSHHKSKKNLDEFLKEYDVCGISDLDTRKLTKKIRTYGAMKAVIAVGDIEIDNEELLKKVKLHKNISELNLVS
DVCVKNPIILGGGKKTVVLIDCGVKKSIVNQLIDKNINLVIVPYNTSYKDILEYNPNGVFISNGPGDPAVLKETINTVKN
LIGKVPIYGICLGHQIISLALGAKTRKLKFGHHGLNQPIKDLENGRVYITSQNHNFAVSDLPDCMDITQINLNDNSIEGI
KHKELPIVGVQYHPEAKPGPHDTYGFFDDFNKIIKQY
>Mature_356_residues
TKAKLILEDGTVMQGKGFGAEGVAVGEIIFCTGMTGYVEALTDPSYKGQILTMTYPLIGNYGVNEEWFESDGIKVGGFVV
KENCLTPSHHKSKKNLDEFLKEYDVCGISDLDTRKLTKKIRTYGAMKAVIAVGDIEIDNEELLKKVKLHKNISELNLVSD
VCVKNPIILGGGKKTVVLIDCGVKKSIVNQLIDKNINLVIVPYNTSYKDILEYNPNGVFISNGPGDPAVLKETINTVKNL
IGKVPIYGICLGHQIISLALGAKTRKLKFGHHGLNQPIKDLENGRVYITSQNHNFAVSDLPDCMDITQINLNDNSIEGIK
HKELPIVGVQYHPEAKPGPHDTYGFFDDFNKIIKQY

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=368, Percent_Identity=41.304347826087, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI169790915, Length=368, Percent_Identity=38.5869565217391, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI21361331, Length=368, Percent_Identity=38.5869565217391, Blast_Score=244, Evalue=1e-64,
Organism=Escherichia coli, GI1786215, Length=375, Percent_Identity=41.8666666666667, Blast_Score=304, Evalue=7e-84,
Organism=Escherichia coli, GI1789760, Length=138, Percent_Identity=30.4347826086957, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=370, Percent_Identity=42.4324324324324, Blast_Score=277, Evalue=5e-75,
Organism=Saccharomyces cerevisiae, GI6324878, Length=380, Percent_Identity=39.2105263157895, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6322331, Length=392, Percent_Identity=39.7959183673469, Blast_Score=259, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6322638, Length=138, Percent_Identity=29.7101449275362, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24642586, Length=381, Percent_Identity=37.7952755905512, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI45555749, Length=381, Percent_Identity=37.7952755905512, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39379; Mature: 39248

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAKLILEDGTVMQGKGFGAEGVAVGEIIFCTGMTGYVEALTDPSYKGQILTMTYPLIG
CCCCEEEEECCCEEECCCCCCCCEEECEEEEECCCHHHHHHHCCCCCCCEEEEEEECCCC
NYGVNEEWFESDGIKVGGFVVKENCLTPSHHKSKKNLDEFLKEYDVCGISDLDTRKLTKK
CCCCCHHHHCCCCEEECCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
IRTYGAMKAVIAVGDIEIDNEELLKKVKLHKNISELNLVSDVCVKNPIILGGGKKTVVLI
HHHHHHHEEEEEECCEEECHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEECCCEEEEEE
DCGVKKSIVNQLIDKNINLVIVPYNTSYKDILEYNPNGVFISNGPGDPAVLKETINTVKN
ECCCHHHHHHHHHHCCCCEEEEECCCCHHHHHEECCCEEEEECCCCCHHHHHHHHHHHHH
LIGKVPIYGICLGHQIISLALGAKTRKLKFGHHGLNQPIKDLENGRVYITSQNHNFAVSD
HHHCCCEEEEEHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHCCCEEEEEECCCCEECCC
LPDCMDITQINLNDNSIEGIKHKELPIVGVQYHPEAKPGPHDTYGFFDDFNKIIKQY
CCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TKAKLILEDGTVMQGKGFGAEGVAVGEIIFCTGMTGYVEALTDPSYKGQILTMTYPLIG
CCCEEEEECCCEEECCCCCCCCEEECEEEEECCCHHHHHHHCCCCCCCEEEEEEECCCC
NYGVNEEWFESDGIKVGGFVVKENCLTPSHHKSKKNLDEFLKEYDVCGISDLDTRKLTKK
CCCCCHHHHCCCCEEECCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
IRTYGAMKAVIAVGDIEIDNEELLKKVKLHKNISELNLVSDVCVKNPIILGGGKKTVVLI
HHHHHHHEEEEEECCEEECHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEECCCEEEEEE
DCGVKKSIVNQLIDKNINLVIVPYNTSYKDILEYNPNGVFISNGPGDPAVLKETINTVKN
ECCCHHHHHHHHHHCCCCEEEEECCCCHHHHHEECCCEEEEECCCCCHHHHHHHHHHHHH
LIGKVPIYGICLGHQIISLALGAKTRKLKFGHHGLNQPIKDLENGRVYITSQNHNFAVSD
HHHCCCEEEEEHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHCCCEEEEEECCCCEECCC
LPDCMDITQINLNDNSIEGIKHKELPIVGVQYHPEAKPGPHDTYGFFDDFNKIIKQY
CCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]