Click here to switch to the map view.

The map label for this gene is neuC [H]

Identifier: 150400855

GI number: 150400855

Start: 442093

End: 443265

Strand: Direct

Name: neuC [H]

Synonym: Maeo_0423

Alternate gene names: 150400855

Gene position: 442093-443265 (Clockwise)

Preceding gene: 150400854

Following gene: 150400856

Centisome position: 28.17

GC content: 30.52

Gene sequence:

>1173_bases
ATGAAAAATAGAAAAATTAAAAAAGTTGCAGTTGTTACAGGAACTCGTGCAGAGTATGGAATTTTGAAACCATTGATTGA
AAAAATTAATGACGATAATGACTTAGAATTGCAATTAATGGTCACTGGTATGCATTTGTTAAAAAAATTTGGATACTCTA
TAAAAGAAATAGAAAACGATGGGTTTCCAATAGCTTCAAAAATAAGAATGTATGATGAAGACTCCCTTGGGGAATTGAGT
TATCATGGTGTTTCATTAGGTAGGGCAGTTTCTGAATTCACAAGAGAATTTGTATATCTAAATCCAGATATTGTTTTAGT
TATTGGGGATAGATTAGAAGCATTAGCTCCTGTATTATCGGCATCAACTTTAAATATTCCAATTGGGCACATTCATGCAG
GGGATAGTACAGACAGTGGACATATTGATGAACAAATTAGATTCGCCATTTCAAGATTTTCCCATTTATTATTTGCACCA
ACTGAAAAGTGTGTAGAACGTTTAACAAAAATGGGGGAAGAACCATGGAGGGCATATAATGTTGGAGCGTTGGGTTTGGA
TAGTATTTTATCATACAAGCCATTAACTAAAGAGGAATTATTTAAAAAATTAAATCTTAATGGAGATAATCCAGTAGCAA
TCATTATATTTCATCCTGTAATTCATGAATACTCAACAATTGACCATCAAATAGAAAGCATTATGAAAGCGGTAATCGAA
ACAAAAATAAATACAGTAGTTATTTATCCAAATAATGACTTAGGCAGTAAGAAAATTATTGAAATTATTAAAAAATATTC
TAATTTTGAAAATATTAAATTATTTGAGAATTTGGAGCACAATATATATATAAGTTTAATGTATCATGCTAATCTAATGA
TTGGAAATTCAAGTAGTGGGATAATTGAAGCACCATCATTAGGATTGCCTGTTATAAATGTAGGAAGTAGAAATACTGGG
AGGGAACATGGGGACAATGTCTTATTTGTAAAACCAGTCAGTAAGGAGATTGTTGAAGCTATTAATAAGGCATTGTATGA
TATTGACTTCATTGAAAAAGTTAAAAAGAAAAACAATCCCTGGGGAGATGGGAAAACCAGCGAAAGACTGGTTAATATTT
TAAAAAATACTGAAATTAATGAGCATTTTATGAGAAAGAGAATCCTATATTAA

Upstream 100 bases:

>100_bases
AATGGAGAGTTTATATATAAAAAGAAAAATAAATTTATAGATTACGAAATAAAGGAAATAAAGGAAATAATTAACATAGT
GTAATGTAGGTGGGTCCCCT

Downstream 100 bases:

>100_bases
TTAATTGAATTTAAAATAACTAAAAAATATAAAATATAATAGTGGTGATATTATGAAAAATTTTAAAGAAGCATGGGAAA
AATTATATGATGAAAATAAA

Product: UDP-N-acetylglucosamine 2-epimerase

Products: UDP-N-acetyl-D-mannosamine [C]

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKNRKIKKVAVVTGTRAEYGILKPLIEKINDDNDLELQLMVTGMHLLKKFGYSIKEIENDGFPIASKIRMYDEDSLGELS
YHGVSLGRAVSEFTREFVYLNPDIVLVIGDRLEALAPVLSASTLNIPIGHIHAGDSTDSGHIDEQIRFAISRFSHLLFAP
TEKCVERLTKMGEEPWRAYNVGALGLDSILSYKPLTKEELFKKLNLNGDNPVAIIIFHPVIHEYSTIDHQIESIMKAVIE
TKINTVVIYPNNDLGSKKIIEIIKKYSNFENIKLFENLEHNIYISLMYHANLMIGNSSSGIIEAPSLGLPVINVGSRNTG
REHGDNVLFVKPVSKEIVEAINKALYDIDFIEKVKKKNNPWGDGKTSERLVNILKNTEINEHFMRKRILY

Sequences:

>Translated_390_residues
MKNRKIKKVAVVTGTRAEYGILKPLIEKINDDNDLELQLMVTGMHLLKKFGYSIKEIENDGFPIASKIRMYDEDSLGELS
YHGVSLGRAVSEFTREFVYLNPDIVLVIGDRLEALAPVLSASTLNIPIGHIHAGDSTDSGHIDEQIRFAISRFSHLLFAP
TEKCVERLTKMGEEPWRAYNVGALGLDSILSYKPLTKEELFKKLNLNGDNPVAIIIFHPVIHEYSTIDHQIESIMKAVIE
TKINTVVIYPNNDLGSKKIIEIIKKYSNFENIKLFENLEHNIYISLMYHANLMIGNSSSGIIEAPSLGLPVINVGSRNTG
REHGDNVLFVKPVSKEIVEAINKALYDIDFIEKVKKKNNPWGDGKTSERLVNILKNTEINEHFMRKRILY
>Mature_390_residues
MKNRKIKKVAVVTGTRAEYGILKPLIEKINDDNDLELQLMVTGMHLLKKFGYSIKEIENDGFPIASKIRMYDEDSLGELS
YHGVSLGRAVSEFTREFVYLNPDIVLVIGDRLEALAPVLSASTLNIPIGHIHAGDSTDSGHIDEQIRFAISRFSHLLFAP
TEKCVERLTKMGEEPWRAYNVGALGLDSILSYKPLTKEELFKKLNLNGDNPVAIIIFHPVIHEYSTIDHQIESIMKAVIE
TKINTVVIYPNNDLGSKKIIEIIKKYSNFENIKLFENLEHNIYISLMYHANLMIGNSSSGIIEAPSLGLPVINVGSRNTG
REHGDNVLFVKPVSKEIVEAINKALYDIDFIEKVKKKNNPWGDGKTSERLVNILKNTEINEHFMRKRILY

Specific function: May be involved in the synthesis of polysialic acid (PSA) [H]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI298566312, Length=395, Percent_Identity=32.4050632911392, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI4885285, Length=395, Percent_Identity=32.4050632911392, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI190014632, Length=395, Percent_Identity=32.4050632911392, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI298566325, Length=383, Percent_Identity=30.5483028720627, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI298566315, Length=336, Percent_Identity=29.4642857142857, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI48994967, Length=397, Percent_Identity=25.1889168765743, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020004
- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: 5.1.3.14 [C]

Molecular weight: Translated: 44087; Mature: 44087

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRKIKKVAVVTGTRAEYGILKPLIEKINDDNDLELQLMVTGMHLLKKFGYSIKEIEND
CCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCHHHHHCCC
GFPIASKIRMYDEDSLGELSYHGVSLGRAVSEFTREFVYLNPDIVLVIGDRLEALAPVLS
CCCHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCEEEEECCHHHHHHHHHC
ASTLNIPIGHIHAGDSTDSGHIDEQIRFAISRFSHLLFAPTEKCVERLTKMGEEPWRAYN
CCEEECEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEE
VGALGLDSILSYKPLTKEELFKKLNLNGDNPVAIIIFHPVIHEYSTIDHQIESIMKAVIE
CCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHH
TKINTVVIYPNNDLGSKKIIEIIKKYSNFENIKLFENLEHNIYISLMYHANLMIGNSSSG
HCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEHHHCCCCCEEEEEEEEEEEEEECCCCC
IIEAPSLGLPVINVGSRNTGREHGDNVLFVKPVSKEIVEAINKALYDIDFIEKVKKKNNP
EEECCCCCCCEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCC
WGDGKTSERLVNILKNTEINEHFMRKRILY
CCCCCHHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKNRKIKKVAVVTGTRAEYGILKPLIEKINDDNDLELQLMVTGMHLLKKFGYSIKEIEND
CCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHCCHHHHHCCC
GFPIASKIRMYDEDSLGELSYHGVSLGRAVSEFTREFVYLNPDIVLVIGDRLEALAPVLS
CCCHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCEEEEECCHHHHHHHHHC
ASTLNIPIGHIHAGDSTDSGHIDEQIRFAISRFSHLLFAPTEKCVERLTKMGEEPWRAYN
CCEEECEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEE
VGALGLDSILSYKPLTKEELFKKLNLNGDNPVAIIIFHPVIHEYSTIDHQIESIMKAVIE
CCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHH
TKINTVVIYPNNDLGSKKIIEIIKKYSNFENIKLFENLEHNIYISLMYHANLMIGNSSSG
HCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEHHHCCCCCEEEEEEEEEEEEEECCCCC
IIEAPSLGLPVINVGSRNTGREHGDNVLFVKPVSKEIVEAINKALYDIDFIEKVKKKNNP
EEECCCCCCCEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCC
WGDGKTSERLVNILKNTEINEHFMRKRILY
CCCCCHHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: UDP-N-acetylglucosamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.63 {UDP-N-acetylglucosamine}} 1 {UDP-N-acetylmannosamine}} 0.5 {UDP-N-acetylmannosamine}} [C]

Substrates: UDP-N-acetyl-D-glucosamine [C]

Specific reaction: UDP-N-acetyl-D-glucosamine --> UDP-N-acetyl-D-mannosamine [C]

General reaction: Epimerization [C]

Inhibitor: PCMB [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1729218 [H]