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The map label for this gene is gph [C]

Identifier: 150400854

GI number: 150400854

Start: 441423

End: 442076

Strand: Direct

Name: gph [C]

Synonym: Maeo_0422

Alternate gene names: 150400854

Gene position: 441423-442076 (Clockwise)

Preceding gene: 150400853

Following gene: 150400855

Centisome position: 28.13

GC content: 24.46

Gene sequence:

>654_bases
ATGAAAGCCATAATATTTGATTTAGATAATACTCTTTATGATTATAGGGATTATTTCTATCAAGTTTTTTTAAAATTATC
AGAATATTTTTATAAAAGGTACCAAATTCCAAAAGATGAATTTATTAAAACATCAATGGAGATATTGAACAAAAGAAAAA
GCAGATATCCAAAATTATTCAATGAAATATTGAATGTATTAAACATTCCTGAAAACGAAGTTAAGTTTTGTGTTGAGATA
TTCACTAGTGGAAGATTTCCAATAGTTCCATCTGATGGAGTTTATGAAGTGTTAGATTATCTAAAAAACAAAAATTATTT
TTTAGGCATAATTACTGATGGAAACCATATACGTCAAAGAGAAAAAATAAAATCCTTAAAATTTGAAAATTATTTTGATA
CTGTTGTTTATACAGATATTTTTCAATCTCCAAAACCTTCCCCAACACCTTACCAATATATAATTAGTAAATTTGGTATT
AATCCAAAATTATCATATTATGTTGGCGATGACCCTGATGTTGATTTTAGAGGAGCAAAATTCGTTGGATTAAATACTAT
AAGGGTATTAAATGGAGAGTTTATATATAAAAAGAAAAATAAATTTATAGATTACGAAATAAAGGAAATAAAGGAAATAA
TTAACATAGTGTAA

Upstream 100 bases:

>100_bases
ATAAGAATTGTCAAAGGGGAAAAACCCATACCAAGCAAAGGTTTCAAACAAAACTTACTGATGTTAAGATACTACTCTGA
AATTTTTGTTGAGGGGAAAC

Downstream 100 bases:

>100_bases
TGTAGGTGGGTCCCCTATGAAAAATAGAAAAATTAAAAAAGTTGCAGTTGTTACAGGAACTCGTGCAGAGTATGGAATTT
TGAAACCATTGATTGAAAAA

Product: HAD family hydrolase

Products: Glycolate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MKAIIFDLDNTLYDYRDYFYQVFLKLSEYFYKRYQIPKDEFIKTSMEILNKRKSRYPKLFNEILNVLNIPENEVKFCVEI
FTSGRFPIVPSDGVYEVLDYLKNKNYFLGIITDGNHIRQREKIKSLKFENYFDTVVYTDIFQSPKPSPTPYQYIISKFGI
NPKLSYYVGDDPDVDFRGAKFVGLNTIRVLNGEFIYKKKNKFIDYEIKEIKEIINIV

Sequences:

>Translated_217_residues
MKAIIFDLDNTLYDYRDYFYQVFLKLSEYFYKRYQIPKDEFIKTSMEILNKRKSRYPKLFNEILNVLNIPENEVKFCVEI
FTSGRFPIVPSDGVYEVLDYLKNKNYFLGIITDGNHIRQREKIKSLKFENYFDTVVYTDIFQSPKPSPTPYQYIISKFGI
NPKLSYYVGDDPDVDFRGAKFVGLNTIRVLNGEFIYKKKNKFIDYEIKEIKEIINIV
>Mature_217_residues
MKAIIFDLDNTLYDYRDYFYQVFLKLSEYFYKRYQIPKDEFIKTSMEILNKRKSRYPKLFNEILNVLNIPENEVKFCVEI
FTSGRFPIVPSDGVYEVLDYLKNKNYFLGIITDGNHIRQREKIKSLKFENYFDTVVYTDIFQSPKPSPTPYQYIISKFGI
NPKLSYYVGDDPDVDFRGAKFVGLNTIRVLNGEFIYKKKNKFIDYEIKEIKEIINIV

Specific function: Unknown

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR011950
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: 3.1.3.18 [C]

Molecular weight: Translated: 25956; Mature: 25956

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIIFDLDNTLYDYRDYFYQVFLKLSEYFYKRYQIPKDEFIKTSMEILNKRKSRYPKLF
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
NEILNVLNIPENEVKFCVEIFTSGRFPIVPSDGVYEVLDYLKNKNYFLGIITDGNHIRQR
HHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHH
EKIKSLKFENYFDTVVYTDIFQSPKPSPTPYQYIISKFGINPKLSYYVGDDPDVDFRGAK
HHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCE
FVGLNTIRVLNGEFIYKKKNKFIDYEIKEIKEIINIV
EECCEEEEEECCEEEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKAIIFDLDNTLYDYRDYFYQVFLKLSEYFYKRYQIPKDEFIKTSMEILNKRKSRYPKLF
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
NEILNVLNIPENEVKFCVEIFTSGRFPIVPSDGVYEVLDYLKNKNYFLGIITDGNHIRQR
HHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHH
EKIKSLKFENYFDTVVYTDIFQSPKPSPTPYQYIISKFGINPKLSYYVGDDPDVDFRGAK
HHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCE
FVGLNTIRVLNGEFIYKKKNKFIDYEIKEIKEIINIV
EECCEEEEEECCEEEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.57 {phosphoglycolate}} [C]

Substrates: D-Glycerate 2-phosphatelyc; H2O [C]

Specific reaction: D-Glycerate 2-phosphatelyc + H2O --> Glycolate + Phosphate [C]

General reaction: Additional information:

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]