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The map label for this gene is rfbB [C]

Identifier: 150400847

GI number: 150400847

Start: 434538

End: 435518

Strand: Direct

Name: rfbB [C]

Synonym: Maeo_0415

Alternate gene names: 150400847

Gene position: 434538-435518 (Clockwise)

Preceding gene: 150400846

Following gene: 150400848

Centisome position: 27.69

GC content: 29.87

Gene sequence:

>981_bases
ATGAAATATAAAAATATTTTAATAACTGGAAGTGCGGGATTTATTGGATTTCATTTAAGCAAATATTTATTGGAAAATTA
TAATAATGTCCAAGTCATTGGGATTGATAATCTAAATAATTACTACAATCCCCTTTTAAAGGAAAAGAGAAATGATATCT
TAAAAAATTATGAAAATTACAACTTTATTAAAGCTGATTTTTCTAACTGGAATGATTTGGAAAATAATCTAAAAAATAAA
GATATTGATTTAATAGTTCATCTCGGAGCTCAGGCTGGTGTGAGGTACTCTTTGGAGAATCCTTGGGCCTATGAAAAATC
AAACCTATTAGGAACGATGAATATATTTGAGCTCGCTAGAAAGCTAAATATAGAAAAGGTTGTTTATGCTTCTTCATCAT
CAGTATATGGAGGGAATAAAAAGATTCCATTTAGTGAAGATGATATCGTGGATAAACCTGTTTCTTTATATGCTGCAACA
AAAAAATCAAATGAATTAATGGCACATACTTACCACCATTTATACGACATTAAGATGATAGGATTAAGATTTTTTACAGT
TTATGGGGAGTATGGAAGACCTGATATGGCATATTTTAAATTTGCAAAAAAAATACTGTCAAATGAACCTATTGACATAT
ATAACTATGGAGATATGGAAAGAGATTTTACCTATATATCCGATGTTGTGGACGGCATAATTTCATCAATTGAAAAAGAT
TTTGATTATGAAATTTTTAATTTGGGAAACTCCCGACCTGTTAAGTTGATGTATTTTGTGGAGCTCCTTGAAAAATACCT
TAACAAAGAGGCAGAAAAGAACTTTTTGCCCATGCAAGATGGGGATGTTTTAAGAACTTATGCGGATTTAAATAAAAGCA
GTAAATTATTAAATTATAATCCAAAGGTTTCTATTGAAGAAGGGCTGAAAAGGTTTTGTAATTGGTTTTTAGAGAATAAG
GAGTGGCTATTGGTGTTGTAA

Upstream 100 bases:

>100_bases
ACAAATTAGTTAAGGATAAAGTTGTATTTGATGGTAGAAATATTCTCGATATGGACAAAATAAAGAAATTGGGATTTGAG
TATTATGGGGTAGGTAGGAA

Downstream 100 bases:

>100_bases
ATATGGACTATAAAGAAAGGGCAATAAAAGGTATTAGTTGGAATTTTCTATTATTAATATTGGCGGCTCCCATTGGCTAT
TTAGTCAGGATGTTATATGC

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKYKNILITGSAGFIGFHLSKYLLENYNNVQVIGIDNLNNYYNPLLKEKRNDILKNYENYNFIKADFSNWNDLENNLKNK
DIDLIVHLGAQAGVRYSLENPWAYEKSNLLGTMNIFELARKLNIEKVVYASSSSVYGGNKKIPFSEDDIVDKPVSLYAAT
KKSNELMAHTYHHLYDIKMIGLRFFTVYGEYGRPDMAYFKFAKKILSNEPIDIYNYGDMERDFTYISDVVDGIISSIEKD
FDYEIFNLGNSRPVKLMYFVELLEKYLNKEAEKNFLPMQDGDVLRTYADLNKSSKLLNYNPKVSIEEGLKRFCNWFLENK
EWLLVL

Sequences:

>Translated_326_residues
MKYKNILITGSAGFIGFHLSKYLLENYNNVQVIGIDNLNNYYNPLLKEKRNDILKNYENYNFIKADFSNWNDLENNLKNK
DIDLIVHLGAQAGVRYSLENPWAYEKSNLLGTMNIFELARKLNIEKVVYASSSSVYGGNKKIPFSEDDIVDKPVSLYAAT
KKSNELMAHTYHHLYDIKMIGLRFFTVYGEYGRPDMAYFKFAKKILSNEPIDIYNYGDMERDFTYISDVVDGIISSIEKD
FDYEIFNLGNSRPVKLMYFVELLEKYLNKEAEKNFLPMQDGDVLRTYADLNKSSKLLNYNPKVSIEEGLKRFCNWFLENK
EWLLVL
>Mature_326_residues
MKYKNILITGSAGFIGFHLSKYLLENYNNVQVIGIDNLNNYYNPLLKEKRNDILKNYENYNFIKADFSNWNDLENNLKNK
DIDLIVHLGAQAGVRYSLENPWAYEKSNLLGTMNIFELARKLNIEKVVYASSSSVYGGNKKIPFSEDDIVDKPVSLYAAT
KKSNELMAHTYHHLYDIKMIGLRFFTVYGEYGRPDMAYFKFAKKILSNEPIDIYNYGDMERDFTYISDVVDGIISSIEKD
FDYEIFNLGNSRPVKLMYFVELLEKYLNKEAEKNFLPMQDGDVLRTYADLNKSSKLLNYNPKVSIEEGLKRFCNWFLENK
EWLLVL

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=322, Percent_Identity=29.1925465838509, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI42516563, Length=329, Percent_Identity=28.5714285714286, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1788353, Length=344, Percent_Identity=24.1279069767442, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786974, Length=336, Percent_Identity=25, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI48994969, Length=341, Percent_Identity=24.0469208211144, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17539532, Length=324, Percent_Identity=27.7777777777778, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17568069, Length=327, Percent_Identity=25.0764525993884, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71982035, Length=348, Percent_Identity=23.8505747126437, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=348, Percent_Identity=24.1379310344828, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI115532424, Length=329, Percent_Identity=21.580547112462, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=351, Percent_Identity=24.7863247863248, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321437, Length=361, Percent_Identity=23.2686980609418, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356223, Length=324, Percent_Identity=31.4814814814815, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI19923002, Length=342, Percent_Identity=25.4385964912281, Blast_Score=101, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46

Molecular weight: Translated: 38089; Mature: 38089

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYKNILITGSAGFIGFHLSKYLLENYNNVQVIGIDNLNNYYNPLLKEKRNDILKNYENY
CCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCC
NFIKADFSNWNDLENNLKNKDIDLIVHLGAQAGVRYSLENPWAYEKSNLLGTMNIFELAR
CEEEECCCCCHHHHHCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCEEHHHHHHHHH
KLNIEKVVYASSSSVYGGNKKIPFSEDDIVDKPVSLYAATKKSNELMAHTYHHLYDIKMI
HCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHEEEECCCCCHHHHHHHHHHHHHEEH
GLRFFTVYGEYGRPDMAYFKFAKKILSNEPIDIYNYGDMERDFTYISDVVDGIISSIEKD
HEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
FDYEIFNLGNSRPVKLMYFVELLEKYLNKEAEKNFLPMQDGDVLRTYADLNKSSKLLNYN
CCEEEEECCCCCCCHHHHHHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHCCCCCCEECCC
PKVSIEEGLKRFCNWFLENKEWLLVL
CCCCHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MKYKNILITGSAGFIGFHLSKYLLENYNNVQVIGIDNLNNYYNPLLKEKRNDILKNYENY
CCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCC
NFIKADFSNWNDLENNLKNKDIDLIVHLGAQAGVRYSLENPWAYEKSNLLGTMNIFELAR
CEEEECCCCCHHHHHCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCCCEEHHHHHHHHH
KLNIEKVVYASSSSVYGGNKKIPFSEDDIVDKPVSLYAATKKSNELMAHTYHHLYDIKMI
HCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHEEEECCCCCHHHHHHHHHHHHHEEH
GLRFFTVYGEYGRPDMAYFKFAKKILSNEPIDIYNYGDMERDFTYISDVVDGIISSIEKD
HEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHC
FDYEIFNLGNSRPVKLMYFVELLEKYLNKEAEKNFLPMQDGDVLRTYADLNKSSKLLNYN
CCEEEEECCCCCCCHHHHHHHHHHHHHCHHHHCCCCCCCCCHHHHHHHHCCCCCCEECCC
PKVSIEEGLKRFCNWFLENKEWLLVL
CCCCHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose

Specific reaction: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]