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The map label for this gene is rfbE [H]

Identifier: 150400821

GI number: 150400821

Start: 402456

End: 403481

Strand: Direct

Name: rfbE [H]

Synonym: Maeo_0389

Alternate gene names: 150400821

Gene position: 402456-403481 (Clockwise)

Preceding gene: 150400820

Following gene: 150400822

Centisome position: 25.64

GC content: 33.14

Gene sequence:

>1026_bases
ATGAAAATTCTTATAACGGGAGGATTAGGATTTTTGGGGAGCAATCTTTCACATGAAAGTATAAACCGCGGATATGAGAC
AATAATAATGGATAATTTTTTTAGAGAAGGGACTACATTAAATTATAACTGGTTAAAGGAGCAGGGGGATTTTACCCTTG
AAAATAAGGACATACGACAGTGGTATGATATAGAAGAAATGATAAAAAAATATAAACCAGATGTTGTGTTCCATACAGCA
GGTCAAGTTGCAATGACCACATCATTGAATAATCCAAGGTTAGATTTTGAAGTTAATGCATTGGGGACTTTTAACTTACT
TGAAGCAATTAGAAAATACAGTCCAGAAACTATGGTATTATTTTCTTCGACAAATAAGGTTTATGGGGATTTAGAATATA
TCAAATATGTGGAAAAAGAAACAAGATATATTGCCCCAGATTATCCAAATGGATTTGATGAGAATTTATGTTTAGATTTC
CATTCGCCTTATGGATGCTCAAAAGGAACAGCAGACCAGTATTTATTAGATTATAATAGAATGTTTGGCATAAAAACAGT
TGTTTTTAGGCATTCTTCAATGTATGGAGGAAGGCAATTTGCCACCTATGATCAAGGATGGATTGGATGGTTCGTTAAAG
AAGCATTAAGGATTAAGAGCGGAGAAATTGAAGGTGTCGAAATACATGGGAATGGAAAACAGGTGAGGGATGTTTTACAT
GCTGATGACATGGTAAATCTGTATTTCAAAACTGTTGAAAATATTGATAAATGTGCAGGTGAAGCGTTTAATATTGGTGG
GGGGATGAACAATAGTTTATCTTTGTTGGAATTATTTGGATTTCTGGAAGATGAACTAAGTATTAATATAAATATAAACA
AAAAGCCTTGGAGATGCAGTGACCAAAAGGTATTTGTTGCAGATGTTGGAAAAATAAATAAATATGCTGGATGGAAACCA
AAGATATCTAAGGAAGAAGGATTAAGAAAAATGATTAAATGGTGTGAGGAAATTTTGGGTGAGTAA

Upstream 100 bases:

>100_bases
TGAACAACACATTTTGGATTGGTGTTTATCCTGCACTGGAAAAGGGACATTTGGATTATGTTTATGATGTGTTTAAGAAA
TATTTAGAAAGGTGAAAAAA

Downstream 100 bases:

>100_bases
TTATGACAGATAAACCACTGTTATCAATTTGTATTCCTACATATAATAGGGCTGAATTTTTAAAGGATGCATTAGATTCA
ATATTGAGGCAGATTAACGA

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: CDP-tyvelose 2-epimerase [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKILITGGLGFLGSNLSHESINRGYETIIMDNFFREGTTLNYNWLKEQGDFTLENKDIRQWYDIEEMIKKYKPDVVFHTA
GQVAMTTSLNNPRLDFEVNALGTFNLLEAIRKYSPETMVLFSSTNKVYGDLEYIKYVEKETRYIAPDYPNGFDENLCLDF
HSPYGCSKGTADQYLLDYNRMFGIKTVVFRHSSMYGGRQFATYDQGWIGWFVKEALRIKSGEIEGVEIHGNGKQVRDVLH
ADDMVNLYFKTVENIDKCAGEAFNIGGGMNNSLSLLELFGFLEDELSINININKKPWRCSDQKVFVADVGKINKYAGWKP
KISKEEGLRKMIKWCEEILGE

Sequences:

>Translated_341_residues
MKILITGGLGFLGSNLSHESINRGYETIIMDNFFREGTTLNYNWLKEQGDFTLENKDIRQWYDIEEMIKKYKPDVVFHTA
GQVAMTTSLNNPRLDFEVNALGTFNLLEAIRKYSPETMVLFSSTNKVYGDLEYIKYVEKETRYIAPDYPNGFDENLCLDF
HSPYGCSKGTADQYLLDYNRMFGIKTVVFRHSSMYGGRQFATYDQGWIGWFVKEALRIKSGEIEGVEIHGNGKQVRDVLH
ADDMVNLYFKTVENIDKCAGEAFNIGGGMNNSLSLLELFGFLEDELSINININKKPWRCSDQKVFVADVGKINKYAGWKP
KISKEEGLRKMIKWCEEILGE
>Mature_341_residues
MKILITGGLGFLGSNLSHESINRGYETIIMDNFFREGTTLNYNWLKEQGDFTLENKDIRQWYDIEEMIKKYKPDVVFHTA
GQVAMTTSLNNPRLDFEVNALGTFNLLEAIRKYSPETMVLFSSTNKVYGDLEYIKYVEKETRYIAPDYPNGFDENLCLDF
HSPYGCSKGTADQYLLDYNRMFGIKTVVFRHSSMYGGRQFATYDQGWIGWFVKEALRIKSGEIEGVEIHGNGKQVRDVLH
ADDMVNLYFKTVENIDKCAGEAFNIGGGMNNSLSLLELFGFLEDELSINININKKPWRCSDQKVFVADVGKINKYAGWKP
KISKEEGLRKMIKWCEEILGE

Specific function: Catalyzes the isomeration of CDP-paratose to CDP- tyvelose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=333, Percent_Identity=25.5255255255255, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI56237023, Length=351, Percent_Identity=24.5014245014245, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI56118217, Length=351, Percent_Identity=24.5014245014245, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI189083684, Length=351, Percent_Identity=24.5014245014245, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI7657641, Length=346, Percent_Identity=25.1445086705202, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI4504031, Length=369, Percent_Identity=23.0352303523035, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI48994969, Length=354, Percent_Identity=29.6610169491525, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=28.169014084507, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1786974, Length=346, Percent_Identity=24.8554913294798, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788366, Length=375, Percent_Identity=23.4666666666667, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17539532, Length=335, Percent_Identity=24.7761194029851, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI133901786, Length=359, Percent_Identity=24.2339832869081, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17539422, Length=359, Percent_Identity=24.2339832869081, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI133901788, Length=361, Percent_Identity=24.3767313019391, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI133901790, Length=361, Percent_Identity=24.3767313019391, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17539424, Length=361, Percent_Identity=24.3767313019391, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71982035, Length=354, Percent_Identity=22.0338983050847, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71982038, Length=356, Percent_Identity=21.9101123595506, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI115532424, Length=345, Percent_Identity=22.3188405797101, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=358, Percent_Identity=23.463687150838, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356223, Length=344, Percent_Identity=25, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24158427, Length=369, Percent_Identity=23.5772357723577, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.10 [H]

Molecular weight: Translated: 39177; Mature: 39177

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILITGGLGFLGSNLSHESINRGYETIIMDNFFREGTTLNYNWLKEQGDFTLENKDIRQ
CEEEEECCCHHHCCCCCHHHHHCCCCEEEEHHHHCCCCEEEEHEECCCCCEEECCCHHHH
WYDIEEMIKKYKPDVVFHTAGQVAMTTSLNNPRLDFEVNALGTFNLLEAIRKYSPETMVL
HCCHHHHHHHHCCCEEEECCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEE
FSSTNKVYGDLEYIKYVEKETRYIAPDYPNGFDENLCLDFHSPYGCSKGTADQYLLDYNR
EECCCCHHHHHHHHHHHHHHCCEECCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHH
MFGIKTVVFRHSSMYGGRQFATYDQGWIGWFVKEALRIKSGEIEGVEIHGNGKQVRDVLH
HHHHEEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
ADDMVNLYFKTVENIDKCAGEAFNIGGGMNNSLSLLELFGFLEDELSINININKKPWRCS
HHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCC
DQKVFVADVGKINKYAGWKPKISKEEGLRKMIKWCEEILGE
CCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILITGGLGFLGSNLSHESINRGYETIIMDNFFREGTTLNYNWLKEQGDFTLENKDIRQ
CEEEEECCCHHHCCCCCHHHHHCCCCEEEEHHHHCCCCEEEEHEECCCCCEEECCCHHHH
WYDIEEMIKKYKPDVVFHTAGQVAMTTSLNNPRLDFEVNALGTFNLLEAIRKYSPETMVL
HCCHHHHHHHHCCCEEEECCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEE
FSSTNKVYGDLEYIKYVEKETRYIAPDYPNGFDENLCLDFHSPYGCSKGTADQYLLDYNR
EECCCCHHHHHHHHHHHHHHCCEECCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHH
MFGIKTVVFRHSSMYGGRQFATYDQGWIGWFVKEALRIKSGEIEGVEIHGNGKQVRDVLH
HHHHEEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
ADDMVNLYFKTVENIDKCAGEAFNIGGGMNNSLSLLELFGFLEDELSINININKKPWRCS
HHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCC
DQKVFVADVGKINKYAGWKPKISKEEGLRKMIKWCEEILGE
CCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2793833; 11677608; 12644504; 12642575 [H]