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The map label for this gene is ksgA

Identifier: 150400809

GI number: 150400809

Start: 389126

End: 389941

Strand: Reverse

Name: ksgA

Synonym: Maeo_0376

Alternate gene names: 150400809

Gene position: 389941-389126 (Counterclockwise)

Preceding gene: 150400824

Following gene: 150400788

Centisome position: 24.84

GC content: 25.86

Gene sequence:

>816_bases
ATGCAGCAAAATAAAAAATTAGGACAATGTTTTTTAAAAGATAAAAATATAGTGAAAAAGGCAATTAATGCAGCCAATAT
AAATAAAAATGATATAGTTTTAGAAATTGGATTGGGAAAAGGAATATTAACAAAAGAATTGGCTAAACAATGTAAAAAAG
TAATAGTTATCGAATTAGATAAAAAATTAGAAATATTTTGGGAAGATATAATTAAAGAATATCCCAATGTTGAAATAATT
TGGAATGACGCTTTAAAAGTTAATTTAAAGGAATTGGGCTTTAATAAAGTTGTGGCAAATTTACCATATCAAATATCTTC
CCCAATTACATTTAAATTGTTAGATTGTGATTTTGAAGTTGCTGTTTTGATGTATCAATATGAATTTGCTAAAAGAATGG
GAGCTCCCTCGGGAACAAAAGAATATAGTAGATTAAGTGTTTCCGTGCAATATAGGGCTGTCGTGGATTATGTATGTAAA
GTTTCGCCTTCTGCATTTTCTCCAAAACCAAAGGTAGATTCTGCAATTGTAAAAATAACTAAAAAAAATGAACCAATACA
TAATATAGACAATTGGGAATTTTTTGATGGATTTAATAGGGCATTGTTTCAGCATAGAAATAAAAATACAAAAAAGGCAC
TTATTCATTCGGCACATGAAATTAACATGGATAGGGATAAATTAAAAGAATTATTAAATTCAAATGAAATAATGGAATTT
AATGATTTATTGGGTAAAAAGGTATATACGCTATCCTTAAAAGAAATAGGTGTTTTATGTAATAATTTATATAAAATAAT
AAATAAATCAAAATAA

Upstream 100 bases:

>100_bases
TTTAATCAAATATTAATTACATATATTATTATAAAAGTAATATTATATATTATTATTTATAAACACAAATAATTAACTTA
TCAAGGTATTTAGTGGAATT

Downstream 100 bases:

>100_bases
TACAAATTATTTTCTTCTTGCTATTTTTCTTGCTATTGCCACTTCCCCAATTTTAATTAATCCCGCGAAGAATTTTTCCA
GCCCTCCGCATGCCCATATT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MQQNKKLGQCFLKDKNIVKKAINAANINKNDIVLEIGLGKGILTKELAKQCKKVIVIELDKKLEIFWEDIIKEYPNVEII
WNDALKVNLKELGFNKVVANLPYQISSPITFKLLDCDFEVAVLMYQYEFAKRMGAPSGTKEYSRLSVSVQYRAVVDYVCK
VSPSAFSPKPKVDSAIVKITKKNEPIHNIDNWEFFDGFNRALFQHRNKNTKKALIHSAHEINMDRDKLKELLNSNEIMEF
NDLLGKKVYTLSLKEIGVLCNNLYKIINKSK

Sequences:

>Translated_271_residues
MQQNKKLGQCFLKDKNIVKKAINAANINKNDIVLEIGLGKGILTKELAKQCKKVIVIELDKKLEIFWEDIIKEYPNVEII
WNDALKVNLKELGFNKVVANLPYQISSPITFKLLDCDFEVAVLMYQYEFAKRMGAPSGTKEYSRLSVSVQYRAVVDYVCK
VSPSAFSPKPKVDSAIVKITKKNEPIHNIDNWEFFDGFNRALFQHRNKNTKKALIHSAHEINMDRDKLKELLNSNEIMEF
NDLLGKKVYTLSLKEIGVLCNNLYKIINKSK
>Mature_271_residues
MQQNKKLGQCFLKDKNIVKKAINAANINKNDIVLEIGLGKGILTKELAKQCKKVIVIELDKKLEIFWEDIIKEYPNVEII
WNDALKVNLKELGFNKVVANLPYQISSPITFKLLDCDFEVAVLMYQYEFAKRMGAPSGTKEYSRLSVSVQYRAVVDYVCK
VSPSAFSPKPKVDSAIVKITKKNEPIHNIDNWEFFDGFNRALFQHRNKNTKKALIHSAHEINMDRDKLKELLNSNEIMEF
NDLLGKKVYTLSLKEIGVLCNNLYKIINKSK

Specific function: Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=221, Percent_Identity=40.2714932126697, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI156415992, Length=298, Percent_Identity=26.510067114094, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1786236, Length=217, Percent_Identity=33.1797235023041, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25146882, Length=256, Percent_Identity=36.328125, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI25141369, Length=244, Percent_Identity=27.8688524590164, Blast_Score=91, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6324989, Length=211, Percent_Identity=36.0189573459716, Blast_Score=125, Evalue=6e-30,
Organism=Drosophila melanogaster, GI21358017, Length=212, Percent_Identity=39.1509433962264, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI21357273, Length=238, Percent_Identity=26.890756302521, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_META3 (A6UTZ1)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324575.1
- ProteinModelPortal:   A6UTZ1
- SMR:   A6UTZ1
- STRING:   A6UTZ1
- GeneID:   5327474
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0376
- eggNOG:   arNOG05894
- HOGENOM:   HBG319664
- OMA:   LAVLMYQ
- ProtClustDB:   PRK14896
- BioCyc:   MAEO419665:MAEO_0376-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31145; Mature: 31145

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 12-12 BINDING 37-37 BINDING 58-58 BINDING 83-83 BINDING 100-100

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQNKKLGQCFLKDKNIVKKAINAANINKNDIVLEIGLGKGILTKELAKQCKKVIVIELD
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHEEEEEEC
KKLEIFWEDIIKEYPNVEIIWNDALKVNLKELGFNKVVANLPYQISSPITFKLLDCDFEV
CHHHHHHHHHHHHCCCEEEEECCCEEEEHHHCCHHHHHHCCCEECCCCEEEEEECCCHHH
AVLMYQYEFAKRMGAPSGTKEYSRLSVSVQYRAVVDYVCKVSPSAFSPKPKVDSAIVKIT
HHHHHHHHHHHHHCCCCCCCHHHEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEE
KKNEPIHNIDNWEFFDGFNRALFQHRNKNTKKALIHSAHEINMDRDKLKELLNSNEIMEF
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHH
NDLLGKKVYTLSLKEIGVLCNNLYKIINKSK
HHHHCCEEEEEEHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQQNKKLGQCFLKDKNIVKKAINAANINKNDIVLEIGLGKGILTKELAKQCKKVIVIELD
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHEEEEEEC
KKLEIFWEDIIKEYPNVEIIWNDALKVNLKELGFNKVVANLPYQISSPITFKLLDCDFEV
CHHHHHHHHHHHHCCCEEEEECCCEEEEHHHCCHHHHHHCCCEECCCCEEEEEECCCHHH
AVLMYQYEFAKRMGAPSGTKEYSRLSVSVQYRAVVDYVCKVSPSAFSPKPKVDSAIVKIT
HHHHHHHHHHHHHCCCCCCCHHHEEEEEEHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEE
KKNEPIHNIDNWEFFDGFNRALFQHRNKNTKKALIHSAHEINMDRDKLKELLNSNEIMEF
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHH
NDLLGKKVYTLSLKEIGVLCNNLYKIINKSK
HHHHCCEEEEEEHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Dimethylation [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA