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The map label for this gene is thiC

Identifier: 150400542

GI number: 150400542

Start: 110872

End: 112152

Strand: Direct

Name: thiC

Synonym: Maeo_0104

Alternate gene names: 150400542

Gene position: 110872-112152 (Clockwise)

Preceding gene: 150400541

Following gene: 150400547

Centisome position: 7.06

GC content: 35.68

Gene sequence:

>1281_bases
ATGACTCAAATGACAGATGCAAAAAACAATATAATTACAAAAGAAATGGAATTTGTAGCAAAAGAAGAAAATATTGCCGT
AGAAAAAATAAGAAAATGGGTGGCAAAAGGATTCGTAGTAATCCCCAAAAATATACATAGAAACACAAAACCAGTTGGAA
TAGGGGACAATTTAAAAACTAAGGTAAATGTAAATCTCGGAACATCAACGGATTGTATAGATATAGATATGGAAATTAGA
AAGGCCATCATCTCGGAAGAATACGGTGCCGATGCTATTATGGATTTAAGCACAGGCGGGGAGCTCCCAGAAATTAGAAA
GCAAATATTAAAAAATACTACACTACCAGTAGGAACTGTGCCAATGTATGAAATAGGAATAGAATCAAAAAATAAATATG
GTAGAGTAATTGATTTTGATGAGGATATTATATTTAACACAATAGAGAGACAGGCAAAAGAAGGAGTAGATTTTATGACT
CTCCACTGTGGAATTACCAAACATACCATAGATACATTAAATAATGATGATAGAATTATGGGTGTTGTTAGTAGGGGAGG
AGCATATATTACGGCATATATAATGCACCACCAAAAAGAAAATCCACTATATAGTCAATTTGATTACCTTCTTGAAATGT
TAAAAGAGCATGATGTTACACTTAGTTTAGGGGACGGAATGAGACCGGGCTGTTTATGTGACCACACAGACAGACCACAA
ATCCAAGAACTTATAGTATTGGGAGAGTTAGTAGATAGATGTAGAGAAGCGGGAGTTCAAGTAATGGTAGAAGGTCCGGG
ACATGTGCCATTAAATAATGTGGAAACAAATATGAAGATTCAAAAAACCCTATGTAAAAATGCTCCATTTTATGTATTGG
GGCCACTTCCAACAGATTTGGCAATGGGATATGACCATATAACTTCTGCAATTGGTGGAGCTCTTGCAGCATATAGTGGA
GCTAACTTTTTATGTTATGTAACTCCTGCGGAGCATGTTAGACTAATGAATGAAGACGATGTAAGAGAAGGATTAATTGC
ATCAAAAATTGCCGCACAGGTTGCAGATGTTGCAAAAGGCAATAAACAGGCATGGGCAAAAGAAAAAGAAATGGCAATTG
CTCGAAAAAATCACGATTGGGAAAAACAATTTGAGTTATCTATTGATAGCGATAAACCTAAAAAAATGAGAGAGGAGCTC
CCGCCAAAAGAAGAAGATGCTTGTAGTGTATGTGGAGAATATTGTGCTCTTTTAATGGTGGAAGAAGCAGGAAAAAGATA
A

Upstream 100 bases:

>100_bases
TTTTATAGGATATATTTTTTAAATATTAGTATGATGGATTATCAATTTTGTTTAATTTTATAATTGTAATTTATAATAAT
GTAATTTACGGTGTTTAATA

Downstream 100 bases:

>100_bases
AAATAATTTTTTAAAATTTTTTTATCAAATATTCTCTTGCAAAACATTTATTATTTTTAACTTCAAATTTTTTTATATTT
TTTTTTATATTATTTTCATT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTQMTDAKNNIITKEMEFVAKEENIAVEKIRKWVAKGFVVIPKNIHRNTKPVGIGDNLKTKVNVNLGTSTDCIDIDMEIR
KAIISEEYGADAIMDLSTGGELPEIRKQILKNTTLPVGTVPMYEIGIESKNKYGRVIDFDEDIIFNTIERQAKEGVDFMT
LHCGITKHTIDTLNNDDRIMGVVSRGGAYITAYIMHHQKENPLYSQFDYLLEMLKEHDVTLSLGDGMRPGCLCDHTDRPQ
IQELIVLGELVDRCREAGVQVMVEGPGHVPLNNVETNMKIQKTLCKNAPFYVLGPLPTDLAMGYDHITSAIGGALAAYSG
ANFLCYVTPAEHVRLMNEDDVREGLIASKIAAQVADVAKGNKQAWAKEKEMAIARKNHDWEKQFELSIDSDKPKKMREEL
PPKEEDACSVCGEYCALLMVEEAGKR

Sequences:

>Translated_426_residues
MTQMTDAKNNIITKEMEFVAKEENIAVEKIRKWVAKGFVVIPKNIHRNTKPVGIGDNLKTKVNVNLGTSTDCIDIDMEIR
KAIISEEYGADAIMDLSTGGELPEIRKQILKNTTLPVGTVPMYEIGIESKNKYGRVIDFDEDIIFNTIERQAKEGVDFMT
LHCGITKHTIDTLNNDDRIMGVVSRGGAYITAYIMHHQKENPLYSQFDYLLEMLKEHDVTLSLGDGMRPGCLCDHTDRPQ
IQELIVLGELVDRCREAGVQVMVEGPGHVPLNNVETNMKIQKTLCKNAPFYVLGPLPTDLAMGYDHITSAIGGALAAYSG
ANFLCYVTPAEHVRLMNEDDVREGLIASKIAAQVADVAKGNKQAWAKEKEMAIARKNHDWEKQFELSIDSDKPKKMREEL
PPKEEDACSVCGEYCALLMVEEAGKR
>Mature_425_residues
TQMTDAKNNIITKEMEFVAKEENIAVEKIRKWVAKGFVVIPKNIHRNTKPVGIGDNLKTKVNVNLGTSTDCIDIDMEIRK
AIISEEYGADAIMDLSTGGELPEIRKQILKNTTLPVGTVPMYEIGIESKNKYGRVIDFDEDIIFNTIERQAKEGVDFMTL
HCGITKHTIDTLNNDDRIMGVVSRGGAYITAYIMHHQKENPLYSQFDYLLEMLKEHDVTLSLGDGMRPGCLCDHTDRPQI
QELIVLGELVDRCREAGVQVMVEGPGHVPLNNVETNMKIQKTLCKNAPFYVLGPLPTDLAMGYDHITSAIGGALAAYSGA
NFLCYVTPAEHVRLMNEDDVREGLIASKIAAQVADVAKGNKQAWAKEKEMAIARKNHDWEKQFELSIDSDKPKKMREELP
PKEEDACSVCGEYCALLMVEEAGKR

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=455, Percent_Identity=40.4395604395604, Blast_Score=336, Evalue=1e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_META3 (A6UT74)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324308.1
- STRING:   A6UT74
- GeneID:   5327383
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0104
- eggNOG:   arNOG04546
- HOGENOM:   HBG285234
- OMA:   VDFMTIH
- ProtClustDB:   PRK13352
- BioCyc:   MAEO419665:MAEO_0104-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 47561; Mature: 47430

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: BINDING 65-65 BINDING 94-94 BINDING 123-123 BINDING 162-162 BINDING 264-264 BINDING 291-291

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQMTDAKNNIITKEMEFVAKEENIAVEKIRKWVAKGFVVIPKNIHRNTKPVGIGDNLKT
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCEE
KVNVNLGTSTDCIDIDMEIRKAIISEEYGADAIMDLSTGGELPEIRKQILKNTTLPVGTV
EEEEEECCCCCEEECCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCC
PMYEIGIESKNKYGRVIDFDEDIIFNTIERQAKEGVDFMTLHCGITKHTIDTLNNDDRIM
CEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCEEE
GVVSRGGAYITAYIMHHQKENPLYSQFDYLLEMLKEHDVTLSLGDGMRPGCLCDHTDRPQ
EEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHH
IQELIVLGELVDRCREAGVQVMVEGPGHVPLNNVETNMKIQKTLCKNAPFYVLGPLPTDL
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCHHH
AMGYDHITSAIGGALAAYSGANFLCYVTPAEHVRLMNEDDVREGLIASKIAAQVADVAKG
HHCHHHHHHHHHHHHHHCCCCCEEEEECCHHHHEECCCCHHHHHHHHHHHHHHHHHHHCC
NKQAWAKEKEMAIARKNHDWEKQFELSIDSDKPKKMREELPPKEEDACSVCGEYCALLMV
CHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
EEAGKR
HCCCCC
>Mature Secondary Structure 
TQMTDAKNNIITKEMEFVAKEENIAVEKIRKWVAKGFVVIPKNIHRNTKPVGIGDNLKT
CCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCEE
KVNVNLGTSTDCIDIDMEIRKAIISEEYGADAIMDLSTGGELPEIRKQILKNTTLPVGTV
EEEEEECCCCCEEECCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCC
PMYEIGIESKNKYGRVIDFDEDIIFNTIERQAKEGVDFMTLHCGITKHTIDTLNNDDRIM
CEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCEEE
GVVSRGGAYITAYIMHHQKENPLYSQFDYLLEMLKEHDVTLSLGDGMRPGCLCDHTDRPQ
EEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHH
IQELIVLGELVDRCREAGVQVMVEGPGHVPLNNVETNMKIQKTLCKNAPFYVLGPLPTDL
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCHHH
AMGYDHITSAIGGALAAYSGANFLCYVTPAEHVRLMNEDDVREGLIASKIAAQVADVAKG
HHCHHHHHHHHHHHHHHCCCCCEEEEECCHHHHEECCCCHHHHHHHHHHHHHHHHHHHCC
NKQAWAKEKEMAIARKNHDWEKQFELSIDSDKPKKMREELPPKEEDACSVCGEYCALLMV
CHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
EEAGKR
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA