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The map label for this gene is pyrI

Identifier: 150400480

GI number: 150400480

Start: 46301

End: 46768

Strand: Direct

Name: pyrI

Synonym: Maeo_0042

Alternate gene names: 150400480

Gene position: 46301-46768 (Clockwise)

Preceding gene: 150400479

Following gene: 150400481

Centisome position: 2.95

GC content: 27.35

Gene sequence:

>468_bases
ATGAACCATTTAGGCGATATTATGAAAAATAGTTTGAAAGTTGAACCAATTGAAAACGGAACTGTTATAGACCATATTAA
GCAGGGCAAGGCTTTGAATGTATATAATGCTTTAGACATAAAAAAAGATAAAACCATAACTATTGCTATGAATGTTCCTT
CGGGCAAAAACCATAAAAAAGACATTTTAAAAATTGAAGGTATAGAACTGTCTAAATATGATGTGGATAAAATAAGCCTT
ATTAGTCCAAATGCCACTATAAATATCATAAAAAATAAAATTGTAATAAATAAATTTAAAGTGGAAATACCAAATACAAT
TGAGGGAATTCTCAAATGCACAAACCCAAATTGTATAACCAATATAGAAAATGTGGCAGGTAAATTTGATGTAGAGGGAA
GGGAACCTTTAAAAATAAGATGTTTTTATTGCGAGAAATTTTTAAATTCTATTGAAGTATATAAATAA

Upstream 100 bases:

>100_bases
GGTATTTTTCAGAAAATATACCATAATAAAGTAAATATATTATTACTAACACTTAACATAAATAGGCGTAATAATTTAGG
ACAGTTATTGAACTTACTAT

Downstream 100 bases:

>100_bases
TCTTTTTATTTAATATATATGATATTATCATTTTTTTAGGATAAATAATAAATAGTAGATAATTATATTATATCATAGAA
CCATCGTAAATCTATGAAGA

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MNHLGDIMKNSLKVEPIENGTVIDHIKQGKALNVYNALDIKKDKTITIAMNVPSGKNHKKDILKIEGIELSKYDVDKISL
ISPNATINIIKNKIVINKFKVEIPNTIEGILKCTNPNCITNIENVAGKFDVEGREPLKIRCFYCEKFLNSIEVYK

Sequences:

>Translated_155_residues
MNHLGDIMKNSLKVEPIENGTVIDHIKQGKALNVYNALDIKKDKTITIAMNVPSGKNHKKDILKIEGIELSKYDVDKISL
ISPNATINIIKNKIVINKFKVEIPNTIEGILKCTNPNCITNIENVAGKFDVEGREPLKIRCFYCEKFLNSIEVYK
>Mature_155_residues
MNHLGDIMKNSLKVEPIENGTVIDHIKQGKALNVYNALDIKKDKTITIAMNVPSGKNHKKDILKIEGIELSKYDVDKISL
ISPNATINIIKNKIVINKFKVEIPNTIEGILKCTNPNCITNIENVAGKFDVEGREPLKIRCFYCEKFLNSIEVYK

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family

Homologues:

Organism=Escherichia coli, GI1790692, Length=139, Percent_Identity=40.2877697841727, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRI_META3 (A6UT12)

Other databases:

- EMBL:   CP000743
- RefSeq:   YP_001324246.1
- ProteinModelPortal:   A6UT12
- SMR:   A6UT12
- STRING:   A6UT12
- GeneID:   5326950
- GenomeReviews:   CP000743_GR
- KEGG:   mae:Maeo_0042
- eggNOG:   arNOG10217
- HOGENOM:   HBG305327
- OMA:   EGTVIDH
- ProtClustDB:   PRK00893
- BioCyc:   MAEO419665:MAEO_0042-MONOMER
- HAMAP:   MF_00002
- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- Gene3D:   G3DSA:2.30.30.20
- Gene3D:   G3DSA:3.30.70.140
- ProDom:   PD006194
- TIGRFAMs:   TIGR00240

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C; SSF54893 Asp_carbamoylTrfase_reg; SSF57825 Asp_transf_reg_C

EC number: 2.1.3.2

Molecular weight: Translated: 17482; Mature: 17482

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHLGDIMKNSLKVEPIENGTVIDHIKQGKALNVYNALDIKKDKTITIAMNVPSGKNHKK
CCHHHHHHHCCEEEEECCCCCEEEHHCCCCEEEEEEEEECCCCCEEEEEEECCCCCCCCC
DILKIEGIELSKYDVDKISLISPNATINIIKNKIVINKFKVEIPNTIEGILKCTNPNCIT
CEEEEECEEECCCCCCEEEEECCCCEEEEEECEEEEEEEEEECCCHHHHHEEECCCCCCC
NIENVAGKFDVEGREPLKIRCFYCEKFLNSIEVYK
CHHHCCCEECCCCCCCEEEEEEEHHHHHHHCCCCC
>Mature Secondary Structure
MNHLGDIMKNSLKVEPIENGTVIDHIKQGKALNVYNALDIKKDKTITIAMNVPSGKNHKK
CCHHHHHHHCCEEEEECCCCCEEEHHCCCCEEEEEEEEECCCCCEEEEEEECCCCCCCCC
DILKIEGIELSKYDVDKISLISPNATINIIKNKIVINKFKVEIPNTIEGILKCTNPNCIT
CEEEEECEEECCCCCCEEEEECCCCEEEEEECEEEEEEEEEECCCHHHHHEEECCCCCCC
NIENVAGKFDVEGREPLKIRCFYCEKFLNSIEVYK
CHHHCCCEECCCCCCCEEEEEEEHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA