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The map label for this gene is topA [H]

Identifier: 150400465

GI number: 150400465

Start: 27044

End: 29605

Strand: Direct

Name: topA [H]

Synonym: Maeo_0026

Alternate gene names: 150400465

Gene position: 27044-29605 (Clockwise)

Preceding gene: 150400463

Following gene: 150400466

Centisome position: 1.72

GC content: 33.14

Gene sequence:

>2562_bases
ATGACTGATTTAATAATCTGCGAAAAACCGAATGTATCAAAAAAAATAGCCGATGCTCTTGGAAAACCTAAAAAGAAATC
AAATGATGGGGTTCCATATTATGAAATAGAACGAAATGGAAAAAAAATAATTGTAGCATCTGCTGTTGGTCATTTATATA
CATTAGAGGAGAAAAAATCCGACGAAAAAGGAAAAAATAAAGGAAGAAATAGCTTTGGGAGATATCCTATTTTTGATATA
AGGTGGGTGCCAGCTTCTACAATAAAAGGAAAGGAATATGTTCAAAAATACATAAATACATTAAATAAATTATCTAAGAA
ATCAAATGATTATTATATTGCTTCGGATTGGGATATTGAGGGGGAATTAATAGGTTATCATGCACTACGATATTGTTGTA
ATGAGGATAACGCAAAAAGAATGAGATTTTCATCACTTACTAAAAAGGATATTATTAAAGCATATGAGCAACCCGATAAT
ATAGATTTTGGGCTTGTTGATGCAGGGGATACAAGGCATAGATTAGATTGGTATTATGGTATCAATATGTCTGTGGCACT
GATGCAATGTATAAGGTCAGTCAATAGATGGCATACAATGAGTACAGGGAGGGTTCAAGGTCCAGCATTATCTTTTTTAG
TGGATAGGGAGCTTGAAATAAGAAAATTTGTGCCTGTGCCATACTGGGTAATAGATGCAAATTTAAAAGATGGATTGATA
GCAATACATGAAAAAGATAAATTTTGGGACGAAAAGGAAGCTAACACAATTTACAACAAAATAAAAGACGAGAAGGAAGC
AATTGTAGCAAATGTTAAAAAAACTAAAAAAACAATTAAACCTAATCCGCCATTTGATTTGGGGGCTCTCCAAAGAGAGG
CACATAGAATATTTAAATTCTCACCAAAGAAAACTCAGGAAATCGCTCAGAAATTGTATGAAAAGGCAATTTGTTCATAT
CCAAGAACAAGCTCCCAAAAACTTCCAAAAGATGATAATTATATTAATGACATATTAAATAAAGTTGTAAATCATAATAT
ATATGGAAAATACGCAAAATCTATAATTGAGGAAGGAAGGAAACCTATTGAGGGGAAAAAGGACGACCCAGCTCACCCCG
CAATTCATTTTGTGGATACTCCAAAAGAGGAGCTCCCACCAGAGGAACAAAAATTATATGATTTAATTGTAAGAAGAACT
CTTGCCGTATTTTGGGATAATGCTGAAAGAGAATATAGCAATATATCGTTGGACATAGGAGGGGAGATTTTTAAACTTAG
TGGCTCACGAACCATTAAAGAAGGTTGGCATGAAATATATTATTTCACTAAATTTGATGAGACGGAGCTCCCTGCCCTAA
GAAAAAATAATAAAATTGTCGTAGAAAAAATCAATATTGAGGAAAAGGAGACCAAACCGCCAAAAAGATATACAATGTCT
TCAATTATTAAAGAGCTTGAAAATAGGGAGCTTGGAACAAAATCCACCCGAGCTGACATTGTAGAAAAATTGGTAAAAAG
AGGCTACTTAATAGAGGATAATTCTTTAACTGTGACGGATTTAGGTATTGGAGTAATAGAAGTTCTTAAAAAATACTGTC
CCGAGATTATAGACGAGCAAATGACAAGAGAGCTTGAAAAGAAATTGGATAGATTGCAAAAAAGAACCATAAAAAAAGAA
ACAGTTTTAAAGGATGCCGAAAAGAAATTAACGGCGATACTTAATGAAGTTAAGAAAAAGGAAAAAGAAATAGGGGCTGA
ATTGGTTGAAAAAGTAGATGCCACAAATAAAACACTTCAAACCATAGGTAAATGTGAATGTGGAGGGGATTTGGGAATAA
TCAGAATAAAAAATAAAAGAAGATTTGTAGGTTGCTCTAACTATCCAGAATGCACCATTGCATACCCATTACCTGCCAAG
GGAAGAATTAAAATACCAAATGAAGTTTGTGAGGTTTGTGGAAAACCTATGTTATTAATTGATAATCAAAAACTTTGTGT
AAATACAGATTGTCCTTCAAAAATATCCGAAGAAGATAAAAAAGAATTGGAAAAATTGGAGAAAAGCGAAAAACCATGCC
CAAAATGTGATGGAACACTTACAGTGAAAAAAGGAGTTTATGGTGTATTTTTAGGTTGCTCTAACTATCCAAAATGTAAA
TACACTGAAAAATTACATAGTAAGAAAGAAGATTCCGAAATTACTGTTGTTGGTGAATGTCCAGACTGTGGAGGGGATTT
GGTGATTAAAAAAGGTAGATTTGGAGAATTTATAGCCTGTTCCAACTATCCAAAATGTAAGCACACTGAAAAGATTAAAA
AAGATGGTAAAAGCAATGCCAAAGCCACCAAAACAGAAAAAACAGAAACTAAAACCACCAAAAAGAAATCCACAAAAGGA
AGCACAACAAAGGAAAAGAAAAAACCAGATAATTCAAACGAAGTTGGTGAATGTCCAGACTGTGGAGGGGATTTGGTGAT
TAAAAAAGGTAGATTTGGAGAATTTATAGCCTGTTCCAACTATCCAAAATGTAAGCACACTGAAAAGATTAAAAAAGATT
AA

Upstream 100 bases:

>100_bases
TTTTTATACTACATATTATTTATTATGGGAGCATATTTGGGAGCTCCCGCCGAGATAAATCTAAATTATAATTATATAAT
ATATTATAAATAGTGAAACC

Downstream 100 bases:

>100_bases
ACAATTTATATTATGGGACCTCCAAAATGTTCTGAAATAAAATTAGTTTAGAATTTTAAGATTTACGAATTATTAATATA
TATGGATATGATATTATGAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 853; Mature: 852

Protein sequence:

>853_residues
MTDLIICEKPNVSKKIADALGKPKKKSNDGVPYYEIERNGKKIIVASAVGHLYTLEEKKSDEKGKNKGRNSFGRYPIFDI
RWVPASTIKGKEYVQKYINTLNKLSKKSNDYYIASDWDIEGELIGYHALRYCCNEDNAKRMRFSSLTKKDIIKAYEQPDN
IDFGLVDAGDTRHRLDWYYGINMSVALMQCIRSVNRWHTMSTGRVQGPALSFLVDRELEIRKFVPVPYWVIDANLKDGLI
AIHEKDKFWDEKEANTIYNKIKDEKEAIVANVKKTKKTIKPNPPFDLGALQREAHRIFKFSPKKTQEIAQKLYEKAICSY
PRTSSQKLPKDDNYINDILNKVVNHNIYGKYAKSIIEEGRKPIEGKKDDPAHPAIHFVDTPKEELPPEEQKLYDLIVRRT
LAVFWDNAEREYSNISLDIGGEIFKLSGSRTIKEGWHEIYYFTKFDETELPALRKNNKIVVEKINIEEKETKPPKRYTMS
SIIKELENRELGTKSTRADIVEKLVKRGYLIEDNSLTVTDLGIGVIEVLKKYCPEIIDEQMTRELEKKLDRLQKRTIKKE
TVLKDAEKKLTAILNEVKKKEKEIGAELVEKVDATNKTLQTIGKCECGGDLGIIRIKNKRRFVGCSNYPECTIAYPLPAK
GRIKIPNEVCEVCGKPMLLIDNQKLCVNTDCPSKISEEDKKELEKLEKSEKPCPKCDGTLTVKKGVYGVFLGCSNYPKCK
YTEKLHSKKEDSEITVVGECPDCGGDLVIKKGRFGEFIACSNYPKCKHTEKIKKDGKSNAKATKTEKTETKTTKKKSTKG
STTKEKKKPDNSNEVGECPDCGGDLVIKKGRFGEFIACSNYPKCKHTEKIKKD

Sequences:

>Translated_853_residues
MTDLIICEKPNVSKKIADALGKPKKKSNDGVPYYEIERNGKKIIVASAVGHLYTLEEKKSDEKGKNKGRNSFGRYPIFDI
RWVPASTIKGKEYVQKYINTLNKLSKKSNDYYIASDWDIEGELIGYHALRYCCNEDNAKRMRFSSLTKKDIIKAYEQPDN
IDFGLVDAGDTRHRLDWYYGINMSVALMQCIRSVNRWHTMSTGRVQGPALSFLVDRELEIRKFVPVPYWVIDANLKDGLI
AIHEKDKFWDEKEANTIYNKIKDEKEAIVANVKKTKKTIKPNPPFDLGALQREAHRIFKFSPKKTQEIAQKLYEKAICSY
PRTSSQKLPKDDNYINDILNKVVNHNIYGKYAKSIIEEGRKPIEGKKDDPAHPAIHFVDTPKEELPPEEQKLYDLIVRRT
LAVFWDNAEREYSNISLDIGGEIFKLSGSRTIKEGWHEIYYFTKFDETELPALRKNNKIVVEKINIEEKETKPPKRYTMS
SIIKELENRELGTKSTRADIVEKLVKRGYLIEDNSLTVTDLGIGVIEVLKKYCPEIIDEQMTRELEKKLDRLQKRTIKKE
TVLKDAEKKLTAILNEVKKKEKEIGAELVEKVDATNKTLQTIGKCECGGDLGIIRIKNKRRFVGCSNYPECTIAYPLPAK
GRIKIPNEVCEVCGKPMLLIDNQKLCVNTDCPSKISEEDKKELEKLEKSEKPCPKCDGTLTVKKGVYGVFLGCSNYPKCK
YTEKLHSKKEDSEITVVGECPDCGGDLVIKKGRFGEFIACSNYPKCKHTEKIKKDGKSNAKATKTEKTETKTTKKKSTKG
STTKEKKKPDNSNEVGECPDCGGDLVIKKGRFGEFIACSNYPKCKHTEKIKKD
>Mature_852_residues
TDLIICEKPNVSKKIADALGKPKKKSNDGVPYYEIERNGKKIIVASAVGHLYTLEEKKSDEKGKNKGRNSFGRYPIFDIR
WVPASTIKGKEYVQKYINTLNKLSKKSNDYYIASDWDIEGELIGYHALRYCCNEDNAKRMRFSSLTKKDIIKAYEQPDNI
DFGLVDAGDTRHRLDWYYGINMSVALMQCIRSVNRWHTMSTGRVQGPALSFLVDRELEIRKFVPVPYWVIDANLKDGLIA
IHEKDKFWDEKEANTIYNKIKDEKEAIVANVKKTKKTIKPNPPFDLGALQREAHRIFKFSPKKTQEIAQKLYEKAICSYP
RTSSQKLPKDDNYINDILNKVVNHNIYGKYAKSIIEEGRKPIEGKKDDPAHPAIHFVDTPKEELPPEEQKLYDLIVRRTL
AVFWDNAEREYSNISLDIGGEIFKLSGSRTIKEGWHEIYYFTKFDETELPALRKNNKIVVEKINIEEKETKPPKRYTMSS
IIKELENRELGTKSTRADIVEKLVKRGYLIEDNSLTVTDLGIGVIEVLKKYCPEIIDEQMTRELEKKLDRLQKRTIKKET
VLKDAEKKLTAILNEVKKKEKEIGAELVEKVDATNKTLQTIGKCECGGDLGIIRIKNKRRFVGCSNYPECTIAYPLPAKG
RIKIPNEVCEVCGKPMLLIDNQKLCVNTDCPSKISEEDKKELEKLEKSEKPCPKCDGTLTVKKGVYGVFLGCSNYPKCKY
TEKLHSKKEDSEITVVGECPDCGGDLVIKKGRFGEFIACSNYPKCKHTEKIKKDGKSNAKATKTEKTETKTTKKKSTKGS
TTKEKKKPDNSNEVGECPDCGGDLVIKKGRFGEFIACSNYPKCKHTEKIKKD

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=686, Percent_Identity=25.801749271137, Blast_Score=197, Evalue=3e-50,
Organism=Homo sapiens, GI4507635, Length=684, Percent_Identity=25.5847953216374, Blast_Score=155, Evalue=2e-37,
Organism=Escherichia coli, GI1787529, Length=882, Percent_Identity=26.7573696145125, Blast_Score=234, Evalue=2e-62,
Organism=Escherichia coli, GI1788061, Length=623, Percent_Identity=25.3611556982343, Blast_Score=170, Evalue=4e-43,
Organism=Escherichia coli, GI48994931, Length=163, Percent_Identity=36.8098159509202, Blast_Score=102, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17555378, Length=622, Percent_Identity=25.4019292604502, Blast_Score=189, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI32563869, Length=780, Percent_Identity=23.2051282051282, Blast_Score=140, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6323263, Length=640, Percent_Identity=25.3125, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24585251, Length=701, Percent_Identity=24.8216833095578, Blast_Score=178, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24640096, Length=597, Percent_Identity=22.9480737018425, Blast_Score=137, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005739
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 97413; Mature: 97282

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLIICEKPNVSKKIADALGKPKKKSNDGVPYYEIERNGKKIIVASAVGHLYTLEEKKS
CCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEEECCCHHEEEHHHCC
DEKGKNKGRNSFGRYPIFDIRWVPASTIKGKEYVQKYINTLNKLSKKSNDYYIASDWDIE
CHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
GELIGYHALRYCCNEDNAKRMRFSSLTKKDIIKAYEQPDNIDFGLVDAGDTRHRLDWYYG
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEEC
INMSVALMQCIRSVNRWHTMSTGRVQGPALSFLVDRELEIRKFVPVPYWVIDANLKDGLI
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHCCCCCEEEEECCCCCCEE
AIHEKDKFWDEKEANTIYNKIKDEKEAIVANVKKTKKTIKPNPPFDLGALQREAHRIFKF
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
SPKKTQEIAQKLYEKAICSYPRTSSQKLPKDDNYINDILNKVVNHNIYGKYAKSIIEEGR
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCC
KPIEGKKDDPAHPAIHFVDTPKEELPPEEQKLYDLIVRRTLAVFWDNAEREYSNISLDIG
CCCCCCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
GEIFKLSGSRTIKEGWHEIYYFTKFDETELPALRKNNKIVVEKINIEEKETKPPKRYTMS
CEEEEECCCCCHHHCHHEEEEEEECCCCCCCHHHCCCEEEEEEECCCCCCCCCCHHHHHH
SIIKELENRELGTKSTRADIVEKLVKRGYLIEDNSLTVTDLGIGVIEVLKKYCPEIIDEQ
HHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHH
MTRELEKKLDRLQKRTIKKETVLKDAEKKLTAILNEVKKKEKEIGAELVEKVDATNKTLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
TIGKCECGGDLGIIRIKNKRRFVGCSNYPECTIAYPLPAKGRIKIPNEVCEVCGKPMLLI
HHHCCCCCCCEEEEEECCCCEEECCCCCCCEEEEECCCCCCCEECCHHHHHHCCCCEEEE
DNQKLCVNTDCPSKISEEDKKELEKLEKSEKPCPKCDGTLTVKKGVYGVFLGCSNYPKCK
CCCCEEECCCCCHHCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEEECCCCCCCC
YTEKLHSKKEDSEITVVGECPDCGGDLVIKKGRFGEFIACSNYPKCKHTEKIKKDGKSNA
HHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCCEEEECCCCCCCHHHHHHHCCCCCC
KATKTEKTETKTTKKKSTKGSTTKEKKKPDNSNEVGECPDCGGDLVIKKGRFGEFIACSN
CCCCCCHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEECC
YPKCKHTEKIKKD
CCCCCCCHHHCCC
>Mature Secondary Structure 
TDLIICEKPNVSKKIADALGKPKKKSNDGVPYYEIERNGKKIIVASAVGHLYTLEEKKS
CCEEEECCCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEEECCCHHEEEHHHCC
DEKGKNKGRNSFGRYPIFDIRWVPASTIKGKEYVQKYINTLNKLSKKSNDYYIASDWDIE
CHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
GELIGYHALRYCCNEDNAKRMRFSSLTKKDIIKAYEQPDNIDFGLVDAGDTRHRLDWYYG
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEEEC
INMSVALMQCIRSVNRWHTMSTGRVQGPALSFLVDRELEIRKFVPVPYWVIDANLKDGLI
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHCCCCCEEEEECCCCCCEE
AIHEKDKFWDEKEANTIYNKIKDEKEAIVANVKKTKKTIKPNPPFDLGALQREAHRIFKF
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
SPKKTQEIAQKLYEKAICSYPRTSSQKLPKDDNYINDILNKVVNHNIYGKYAKSIIEEGR
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCC
KPIEGKKDDPAHPAIHFVDTPKEELPPEEQKLYDLIVRRTLAVFWDNAEREYSNISLDIG
CCCCCCCCCCCCCEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEC
GEIFKLSGSRTIKEGWHEIYYFTKFDETELPALRKNNKIVVEKINIEEKETKPPKRYTMS
CEEEEECCCCCHHHCHHEEEEEEECCCCCCCHHHCCCEEEEEEECCCCCCCCCCHHHHHH
SIIKELENRELGTKSTRADIVEKLVKRGYLIEDNSLTVTDLGIGVIEVLKKYCPEIIDEQ
HHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHH
MTRELEKKLDRLQKRTIKKETVLKDAEKKLTAILNEVKKKEKEIGAELVEKVDATNKTLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
TIGKCECGGDLGIIRIKNKRRFVGCSNYPECTIAYPLPAKGRIKIPNEVCEVCGKPMLLI
HHHCCCCCCCEEEEEECCCCEEECCCCCCCEEEEECCCCCCCEECCHHHHHHCCCCEEEE
DNQKLCVNTDCPSKISEEDKKELEKLEKSEKPCPKCDGTLTVKKGVYGVFLGCSNYPKCK
CCCCEEECCCCCHHCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEEECCCCCCCC
YTEKLHSKKEDSEITVVGECPDCGGDLVIKKGRFGEFIACSNYPKCKHTEKIKKDGKSNA
HHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCCEEEECCCCCCCHHHHHHHCCCCCC
KATKTEKTETKTTKKKSTKGSTTKEKKKPDNSNEVGECPDCGGDLVIKKGRFGEFIACSN
CCCCCCHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEECC
YPKCKHTEKIKKD
CCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]