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The map label for this gene is pifA [H]

Identifier: 150400376

GI number: 150400376

Start: 1656608

End: 1659196

Strand: Reverse

Name: pifA [H]

Synonym: Mevan_1639

Alternate gene names: 150400376

Gene position: 1659196-1656608 (Counterclockwise)

Preceding gene: 150400377

Following gene: 150400375

Centisome position: 96.46

GC content: 28.78

Gene sequence:

>2589_bases
TTGGGAAATAACAATATTTTAATGGAGATAAACAATCCTGAAAAAAAAGACCTTCTTGAAAGAGAAGGATTTCATAAAAG
TCTTGCAGAAATAATGAACTGGCAAGATAATGAAAAAAATGGATTTGTTGTTGGACTTTATGGATCATGGGGTTCAGGGA
AATCCTTTTCGATTGATAAATCTTTGGAAATTCTAGAAAATGATTTTAAAGAAAAAATTAATGAATGCAAAAATTCTAAA
CAAAACCGAATTAAAAGATTTATAAAACTCAAAAGAATTGAAAAATGTAATATGCTTACTATAAAATTTAATCCGTGGTA
TTTTACGGAAACTGATGATTTAATATTAAATTTCTTTAAATGTCTTTCGGAATCATTGAAAACACGTCAATCCATATTAG
AAAATATAAAAGATTGGATTGGAAAAATTTTTGGGGTTATGTGGGGATTGGTATCTAGTATTGATAAATATTTAGAAAAA
ATGAGTCCTGAATTCATTACACTGAATCCAATCCTTACAATTACGGTCACAATTGGAAAAATAATCACTAAATGTTTATC
CGTAATATTGAAGGGATACAGACAACCTACCGACATCGTAACCGTAAAAAAGAAATTAGACAAAAAATTCGAAAACTTGC
CATTCAGAATCTTGGTTATTATGGATGACATGGATAGACTGAATGACAGAGAAATCGGTCAAATACTCCATCTTGTAAAA
TGCCTTGCTGATTTTAAAAATATAACTTACGTCCTATCATTTGATGAAAAAATTGTATCTAATGCTTTAGAAAATCATCA
AAATGGATATGGGATGCACTATCTTGAAAAAATTATACAATTTCCAATAAAAATGCCTGAACCCCATACATTAGATTTAG
AAAACATTTTGGAAGAAGAAATAAAAAATATAATTGGAGAAAATCCTCGAGATATACCCCAATACTTTGAATTTTATCCC
TTGTGCTTTAGCAACATGATCAAAAATATTCGAGATGTGAATAGATATATCTCTATTTTTAATTTTAATTGGAATCTTCT
AAAAAATCAAGTTAATAAAATAGATTTAATTGTTTTAACTGCTTTGCAAGTTTTTACGCCCGAAATTTACGATTGGGTAA
AACATAATAAAGAACTTTTTGTAGGTTATTCTGTATTTGATGGAACGTGCGTATTAGGTGGTTCGGGATACGATCATAAA
TATTACAAAAATAAAATCAACGAATACTTGAAAAATTACCGTTCGATCGATGCAGATGATGTTTATCAATTATTAACCGT
TTTATTTCCTAATTCTGCAATTAAAGAATTTGCAATACTTGATACACGTGACGGAAACCCTGAAAACAGGATTTACGGGG
AAAACTCATTCAACAATTATTTTATGTATTGTAAATCAGAACATGAATTTCAGACGTATGAATTAGAATTATATAAAAAA
CAAGGACTCACTGAAAAAGATTTTGAAGGTTTTTTGCACAAATTATCAAAAAGTGATCGTCTAGAACGGTATATCCTAAA
ATTACAAAATAACGGCAACTCACTATATCGAGTACCTCCGAAAAATAGAACAATAATTGCAAATGGGCTTATTTATTCAT
TAGAACATGTAAAACACTATTCAAAAAAGTATATTTCAGGACAAATCGTTAGGGGATTACATGACTTATTTTCACCAACT
AGCGATGACCTCATAATGAATACTCATATTTTAATAAATTCTTTGAAAAAAAGTAATGATTCATATCTGGTTATTGACGA
AATAATTAGAAGATCTAAAAATAAAAATGATCGGTTAACTGATGTTTATTCCCCTGACGAATTAATAAATTTAGATTTTG
AATATCTTGAAAATAACAAATTGGTAGAAATTATCAATGAACTCTATACTTTTGATGAGGTTAAACATTTTGAGACGATT
GAAGCCATTCTTTCGGGAATTAACAAAGAACAGATGCTTTTTATATTGGAAAATATTGACCATGACCTCATTTTGGACTG
TATGAAAAATGTAAAGGATCTTTCGGGAGTCAATATTGGTGCAACGTTAACATTGTTAAAAAAAGACATGAAAAATGTAT
ATGCTGAACTCAACCTTCGTGAAATTACTCCTCCAGAACATTTAACGATATTAACAGGCATAGATGATAAATCTGACCCA
TATATTAATCAAAATGCAAAATATTTGCATGATATTAACAAAAACATACTTGAAAAATATAGTTTACTTGTTGAACAGTA
TCTAGCGTCCATTGCCCCATTTAATACATATCAAAAATTCAAAGAAATCAAGTATGACAATATCAAACATGAAATATTAA
ATATACATCACGGTAATTTCCTCCAAATTTCAAAAATAATATCTGAGTTAAATGAGCCCGAATGTGGATTTTTAATTAGT
GGACTCAGTGAATTACTAATAAAAGAAATAGCTTCACACCTGCCGTCTGGAGAAGGCCCGAAACTATATGAAAAAATAAC
TAGAATCCCGAAACTAAGAGCATTTACTCAACTTGACGAGATTTCTATTGAAAAAATTAATCTATTTATTGAAAATACAT
ATTATTATAAAAATCCCGCTTTAAAGTGA

Upstream 100 bases:

>100_bases
GAATAAAAAAACTGGCGTATTTGATATCATAATCAAAGGAATATATGCAGAAAAAATTGAAGATGAAAAATAAAGACTTA
CATAAAGAGGGGGGGGAATT

Downstream 100 bases:

>100_bases
GCACCGGCACTAACGCCAAACCCTAATTTATATCCTATCCGACCAACGACCACACGAAAACCTATGAATTTCATTGCTTA
GGATGTTTTCGAGCGAAGCG

Product: KAP P-loop domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 862; Mature: 861

Protein sequence:

>862_residues
MGNNNILMEINNPEKKDLLEREGFHKSLAEIMNWQDNEKNGFVVGLYGSWGSGKSFSIDKSLEILENDFKEKINECKNSK
QNRIKRFIKLKRIEKCNMLTIKFNPWYFTETDDLILNFFKCLSESLKTRQSILENIKDWIGKIFGVMWGLVSSIDKYLEK
MSPEFITLNPILTITVTIGKIITKCLSVILKGYRQPTDIVTVKKKLDKKFENLPFRILVIMDDMDRLNDREIGQILHLVK
CLADFKNITYVLSFDEKIVSNALENHQNGYGMHYLEKIIQFPIKMPEPHTLDLENILEEEIKNIIGENPRDIPQYFEFYP
LCFSNMIKNIRDVNRYISIFNFNWNLLKNQVNKIDLIVLTALQVFTPEIYDWVKHNKELFVGYSVFDGTCVLGGSGYDHK
YYKNKINEYLKNYRSIDADDVYQLLTVLFPNSAIKEFAILDTRDGNPENRIYGENSFNNYFMYCKSEHEFQTYELELYKK
QGLTEKDFEGFLHKLSKSDRLERYILKLQNNGNSLYRVPPKNRTIIANGLIYSLEHVKHYSKKYISGQIVRGLHDLFSPT
SDDLIMNTHILINSLKKSNDSYLVIDEIIRRSKNKNDRLTDVYSPDELINLDFEYLENNKLVEIINELYTFDEVKHFETI
EAILSGINKEQMLFILENIDHDLILDCMKNVKDLSGVNIGATLTLLKKDMKNVYAELNLREITPPEHLTILTGIDDKSDP
YINQNAKYLHDINKNILEKYSLLVEQYLASIAPFNTYQKFKEIKYDNIKHEILNIHHGNFLQISKIISELNEPECGFLIS
GLSELLIKEIASHLPSGEGPKLYEKITRIPKLRAFTQLDEISIEKINLFIENTYYYKNPALK

Sequences:

>Translated_862_residues
MGNNNILMEINNPEKKDLLEREGFHKSLAEIMNWQDNEKNGFVVGLYGSWGSGKSFSIDKSLEILENDFKEKINECKNSK
QNRIKRFIKLKRIEKCNMLTIKFNPWYFTETDDLILNFFKCLSESLKTRQSILENIKDWIGKIFGVMWGLVSSIDKYLEK
MSPEFITLNPILTITVTIGKIITKCLSVILKGYRQPTDIVTVKKKLDKKFENLPFRILVIMDDMDRLNDREIGQILHLVK
CLADFKNITYVLSFDEKIVSNALENHQNGYGMHYLEKIIQFPIKMPEPHTLDLENILEEEIKNIIGENPRDIPQYFEFYP
LCFSNMIKNIRDVNRYISIFNFNWNLLKNQVNKIDLIVLTALQVFTPEIYDWVKHNKELFVGYSVFDGTCVLGGSGYDHK
YYKNKINEYLKNYRSIDADDVYQLLTVLFPNSAIKEFAILDTRDGNPENRIYGENSFNNYFMYCKSEHEFQTYELELYKK
QGLTEKDFEGFLHKLSKSDRLERYILKLQNNGNSLYRVPPKNRTIIANGLIYSLEHVKHYSKKYISGQIVRGLHDLFSPT
SDDLIMNTHILINSLKKSNDSYLVIDEIIRRSKNKNDRLTDVYSPDELINLDFEYLENNKLVEIINELYTFDEVKHFETI
EAILSGINKEQMLFILENIDHDLILDCMKNVKDLSGVNIGATLTLLKKDMKNVYAELNLREITPPEHLTILTGIDDKSDP
YINQNAKYLHDINKNILEKYSLLVEQYLASIAPFNTYQKFKEIKYDNIKHEILNIHHGNFLQISKIISELNEPECGFLIS
GLSELLIKEIASHLPSGEGPKLYEKITRIPKLRAFTQLDEISIEKINLFIENTYYYKNPALK
>Mature_861_residues
GNNNILMEINNPEKKDLLEREGFHKSLAEIMNWQDNEKNGFVVGLYGSWGSGKSFSIDKSLEILENDFKEKINECKNSKQ
NRIKRFIKLKRIEKCNMLTIKFNPWYFTETDDLILNFFKCLSESLKTRQSILENIKDWIGKIFGVMWGLVSSIDKYLEKM
SPEFITLNPILTITVTIGKIITKCLSVILKGYRQPTDIVTVKKKLDKKFENLPFRILVIMDDMDRLNDREIGQILHLVKC
LADFKNITYVLSFDEKIVSNALENHQNGYGMHYLEKIIQFPIKMPEPHTLDLENILEEEIKNIIGENPRDIPQYFEFYPL
CFSNMIKNIRDVNRYISIFNFNWNLLKNQVNKIDLIVLTALQVFTPEIYDWVKHNKELFVGYSVFDGTCVLGGSGYDHKY
YKNKINEYLKNYRSIDADDVYQLLTVLFPNSAIKEFAILDTRDGNPENRIYGENSFNNYFMYCKSEHEFQTYELELYKKQ
GLTEKDFEGFLHKLSKSDRLERYILKLQNNGNSLYRVPPKNRTIIANGLIYSLEHVKHYSKKYISGQIVRGLHDLFSPTS
DDLIMNTHILINSLKKSNDSYLVIDEIIRRSKNKNDRLTDVYSPDELINLDFEYLENNKLVEIINELYTFDEVKHFETIE
AILSGINKEQMLFILENIDHDLILDCMKNVKDLSGVNIGATLTLLKKDMKNVYAELNLREITPPEHLTILTGIDDKSDPY
INQNAKYLHDINKNILEKYSLLVEQYLASIAPFNTYQKFKEIKYDNIKHEILNIHHGNFLQISKIISELNEPECGFLISG
LSELLIKEIASHLPSGEGPKLYEKITRIPKLRAFTQLDEISIEKINLFIENTYYYKNPALK

Specific function: Responsible for the exclusion of phage T7 by plasmid F. Growth of bacteriophage T7 is inhibited in cells of E.coli that carries the plasmid F [H]

COG id: COG4928

COG function: function code R; Predicted P-loop ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 KAP NTPase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011646 [H]

Pfam domain/function: PF07693 KAP_NTPase [H]

EC number: NA

Molecular weight: Translated: 100868; Mature: 100736

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNNNILMEINNPEKKDLLEREGFHKSLAEIMNWQDNEKNGFVVGLYGSWGSGKSFSIDK
CCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHH
SLEILENDFKEKINECKNSKQNRIKRFIKLKRIEKCNMLTIKFNPWYFTETDDLILNFFK
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCEEECCHHHHHHHHHH
CLSESLKTRQSILENIKDWIGKIFGVMWGLVSSIDKYLEKMSPEFITLNPILTITVTIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEHHHH
IITKCLSVILKGYRQPTDIVTVKKKLDKKFENLPFRILVIMDDMDRLNDREIGQILHLVK
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCHHCCCHHHHHHHHHHHH
CLADFKNITYVLSFDEKIVSNALENHQNGYGMHYLEKIIQFPIKMPEPHTLDLENILEEE
HHHHHHCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
IKNIIGENPRDIPQYFEFYPLCFSNMIKNIRDVNRYISIFNFNWNLLKNQVNKIDLIVLT
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHH
ALQVFTPEIYDWVKHNKELFVGYSVFDGTCVLGGSGYDHKYYKNKINEYLKNYRSIDADD
HHHHHCHHHHHHHHCCCEEEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHH
VYQLLTVLFPNSAIKEFAILDTRDGNPENRIYGENSFNNYFMYCKSEHEFQTYELELYKK
HHHHHHHHCCCHHHHHHEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
QGLTEKDFEGFLHKLSKSDRLERYILKLQNNGNSLYRVPPKNRTIIANGLIYSLEHVKHY
CCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEECHHHHHHHHHHHH
SKKYISGQIVRGLHDLFSPTSDDLIMNTHILINSLKKSNDSYLVIDEIIRRSKNKNDRLT
HHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCCCC
DVYSPDELINLDFEYLENNKLVEIINELYTFDEVKHFETIEAILSGINKEQMLFILENID
CCCCCHHHEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHCC
HDLILDCMKNVKDLSGVNIGATLTLLKKDMKNVYAELNLREITPPEHLTILTGIDDKSDP
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCC
YINQNAKYLHDINKNILEKYSLLVEQYLASIAPFNTYQKFKEIKYDNIKHEILNIHHGNF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHCCCCCCH
LQISKIISELNEPECGFLISGLSELLIKEIASHLPSGEGPKLYEKITRIPKLRAFTQLDE
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHH
ISIEKINLFIENTYYYKNPALK
CCCEEEEEEEEEEEEECCCCCC
>Mature Secondary Structure 
GNNNILMEINNPEKKDLLEREGFHKSLAEIMNWQDNEKNGFVVGLYGSWGSGKSFSIDK
CCCCEEEEECCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCHH
SLEILENDFKEKINECKNSKQNRIKRFIKLKRIEKCNMLTIKFNPWYFTETDDLILNFFK
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCEEECCHHHHHHHHHH
CLSESLKTRQSILENIKDWIGKIFGVMWGLVSSIDKYLEKMSPEFITLNPILTITVTIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEHHHH
IITKCLSVILKGYRQPTDIVTVKKKLDKKFENLPFRILVIMDDMDRLNDREIGQILHLVK
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCHHCCCHHHHHHHHHHHH
CLADFKNITYVLSFDEKIVSNALENHQNGYGMHYLEKIIQFPIKMPEPHTLDLENILEEE
HHHHHHCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
IKNIIGENPRDIPQYFEFYPLCFSNMIKNIRDVNRYISIFNFNWNLLKNQVNKIDLIVLT
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHH
ALQVFTPEIYDWVKHNKELFVGYSVFDGTCVLGGSGYDHKYYKNKINEYLKNYRSIDADD
HHHHHCHHHHHHHHCCCEEEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHH
VYQLLTVLFPNSAIKEFAILDTRDGNPENRIYGENSFNNYFMYCKSEHEFQTYELELYKK
HHHHHHHHCCCHHHHHHEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHH
QGLTEKDFEGFLHKLSKSDRLERYILKLQNNGNSLYRVPPKNRTIIANGLIYSLEHVKHY
CCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEECHHHHHHHHHHHH
SKKYISGQIVRGLHDLFSPTSDDLIMNTHILINSLKKSNDSYLVIDEIIRRSKNKNDRLT
HHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHCCCCCEEEHHHHHHHCCCCCCCCC
DVYSPDELINLDFEYLENNKLVEIINELYTFDEVKHFETIEAILSGINKEQMLFILENID
CCCCCHHHEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEHHHHHCC
HDLILDCMKNVKDLSGVNIGATLTLLKKDMKNVYAELNLREITPPEHLTILTGIDDKSDP
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCC
YINQNAKYLHDINKNILEKYSLLVEQYLASIAPFNTYQKFKEIKYDNIKHEILNIHHGNF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHCCCCCCH
LQISKIISELNEPECGFLISGLSELLIKEIASHLPSGEGPKLYEKITRIPKLRAFTQLDE
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHHH
ISIEKINLFIENTYYYKNPALK
CCCEEEEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 5283385 [H]