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The map label for this gene is rlmE

Identifier: 150400217

GI number: 150400217

Start: 1500557

End: 1501336

Strand: Reverse

Name: rlmE

Synonym: Mevan_1478

Alternate gene names: 150400217

Gene position: 1501336-1500557 (Counterclockwise)

Preceding gene: 150400218

Following gene: 150400213

Centisome position: 87.28

GC content: 33.33

Gene sequence:

>780_bases
ATGGGAAAAAAGGATAAAAGATGGGTTCTTCAAAGAAAAAAAGACCCTTATTATAATTTAGCTAAGCGGAAAAATTACCG
TTCAAGAGCAACATATAAGTTATTCCAGTTAAATGAAAAATTTAATATAATTAAAGAAAAAAATGTCGTGGTAGATTTAG
GCTGTGCGCCCGGAGGTTGGCTCCAAGCAGCAAGAGACATGACGGGTGAAGAAGGATTTATTGTTGGAATTGATTTACAG
CAGATCAAACCCCTTCCTTATGAAAATGTTATTGCAGTTAAAGGCGATATGACTGATGAAGAAACTTTAAAAAAGATTCA
AGACATACTTCCTGAAAAGCCTGATGTCATTATATGCGATGCATCACCAAATATTAGTGGAGTATGGGATGTTGACCATA
CAAGGTCTCTTGAACTTACAACAATGGCACTGATGACGGCTACAAAAATGCTTAAAAAAGGGGGAAATTTTGTAGTTAAG
GTTTTTCAAGGTGATTTATTCTATAAATATGTAGAATTAGTTTCAGAATACTTTGATAAAGCATTTACAACAAAACCGAG
GGCTTCAAGGGAAGAAAGTGCGGAAGTTTACGTAATTGCAAAGCACTATAATGGTAAAAAATTTAACATGAAATCAAAGT
CAGACATTGTAAAACTTTTAAAGCCGCAAGACGAATTGAAACGAGAAGAATCCGCCTTAAGTCTTCGTAAAAATATTTCA
GATGAAGATACAGGTATGATTATTAAAAAAATAAAACAATTGAGGGCTAAAAAAGATTAA

Upstream 100 bases:

>100_bases
CCCATTGAAGTAAAAACGCTGCACAAATCAATGGATTACTCAAATCTTACAAATTATGAAGAAATTTAAATTAATTAGAT
ATTTAGTTTAAGGAGATAAA

Downstream 100 bases:

>100_bases
TTGCCCTTAAAATCTAATATTTTTAAAACCAATATTTTTAAAATTTAAAGAAAACAAAGTTGTTAAAAATTCAAATAAAT
AAATTTCAAAATAAAAAAGA

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MGKKDKRWVLQRKKDPYYNLAKRKNYRSRATYKLFQLNEKFNIIKEKNVVVDLGCAPGGWLQAARDMTGEEGFIVGIDLQ
QIKPLPYENVIAVKGDMTDEETLKKIQDILPEKPDVIICDASPNISGVWDVDHTRSLELTTMALMTATKMLKKGGNFVVK
VFQGDLFYKYVELVSEYFDKAFTTKPRASREESAEVYVIAKHYNGKKFNMKSKSDIVKLLKPQDELKREESALSLRKNIS
DEDTGMIIKKIKQLRAKKD

Sequences:

>Translated_259_residues
MGKKDKRWVLQRKKDPYYNLAKRKNYRSRATYKLFQLNEKFNIIKEKNVVVDLGCAPGGWLQAARDMTGEEGFIVGIDLQ
QIKPLPYENVIAVKGDMTDEETLKKIQDILPEKPDVIICDASPNISGVWDVDHTRSLELTTMALMTATKMLKKGGNFVVK
VFQGDLFYKYVELVSEYFDKAFTTKPRASREESAEVYVIAKHYNGKKFNMKSKSDIVKLLKPQDELKREESALSLRKNIS
DEDTGMIIKKIKQLRAKKD
>Mature_258_residues
GKKDKRWVLQRKKDPYYNLAKRKNYRSRATYKLFQLNEKFNIIKEKNVVVDLGCAPGGWLQAARDMTGEEGFIVGIDLQQ
IKPLPYENVIAVKGDMTDEETLKKIQDILPEKPDVIICDASPNISGVWDVDHTRSLELTTMALMTATKMLKKGGNFVVKV
FQGDLFYKYVELVSEYFDKAFTTKPRASREESAEVYVIAKHYNGKKFNMKSKSDIVKLLKPQDELKREESALSLRKNISD
EDTGMIIKKIKQLRAKKD

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI194097365, Length=204, Percent_Identity=39.2156862745098, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI7019377, Length=213, Percent_Identity=34.7417840375587, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI29029591, Length=196, Percent_Identity=37.2448979591837, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI29029589, Length=196, Percent_Identity=37.2448979591837, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI7110661, Length=196, Percent_Identity=37.2448979591837, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1789569, Length=198, Percent_Identity=37.3737373737374, Blast_Score=128, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17553860, Length=204, Percent_Identity=40.6862745098039, Blast_Score=151, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71987561, Length=204, Percent_Identity=40.6862745098039, Blast_Score=151, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI71987550, Length=204, Percent_Identity=40.6862745098039, Blast_Score=151, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17554650, Length=199, Percent_Identity=37.1859296482412, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17553474, Length=202, Percent_Identity=31.1881188118812, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319796, Length=195, Percent_Identity=37.4358974358974, Blast_Score=135, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6319535, Length=218, Percent_Identity=36.2385321100917, Blast_Score=130, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321302, Length=280, Percent_Identity=27.8571428571429, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI18859957, Length=204, Percent_Identity=37.7450980392157, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24648639, Length=200, Percent_Identity=31.5, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24647580, Length=214, Percent_Identity=31.3084112149533, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21356387, Length=212, Percent_Identity=28.3018867924528, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_METVS (A6USA0)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001323984.1
- ProteinModelPortal:   A6USA0
- SMR:   A6USA0
- STRING:   A6USA0
- GeneID:   5324858
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_1478
- eggNOG:   arNOG05244
- HOGENOM:   HBG398270
- OMA:   DASPNIS
- ProtClustDB:   CLSK876556
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 29794; Mature: 29663

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: ACT_SITE 160-160 BINDING 58-58 BINDING 60-60 BINDING 78-78 BINDING 96-96 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKDKRWVLQRKKDPYYNLAKRKNYRSRATYKLFQLNEKFNIIKEKNVVVDLGCAPGGW
CCCCCHHHHHHCCCCCHHHHHHHCCCHHHHEEEEEEECCHHCEEECCCEEEEECCCCCHH
LQAARDMTGEEGFIVGIDLQQIKPLPYENVIAVKGDMTDEETLKKIQDILPEKPDVIICD
HHHHHHCCCCCCEEEEEEHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEE
ASPNISGVWDVDHTRSLELTTMALMTATKMLKKGGNFVVKVFQGDLFYKYVELVSEYFDK
CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
AFTTKPRASREESAEVYVIAKHYNGKKFNMKSKSDIVKLLKPQDELKREESALSLRKNIS
HHCCCCCCCCCCCCEEEEEEEECCCCEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCC
DEDTGMIIKKIKQLRAKKD
CCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GKKDKRWVLQRKKDPYYNLAKRKNYRSRATYKLFQLNEKFNIIKEKNVVVDLGCAPGGW
CCCCHHHHHHCCCCCHHHHHHHCCCHHHHEEEEEEECCHHCEEECCCEEEEECCCCCHH
LQAARDMTGEEGFIVGIDLQQIKPLPYENVIAVKGDMTDEETLKKIQDILPEKPDVIICD
HHHHHHCCCCCCEEEEEEHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEE
ASPNISGVWDVDHTRSLELTTMALMTATKMLKKGGNFVVKVFQGDLFYKYVELVSEYFDK
CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHH
AFTTKPRASREESAEVYVIAKHYNGKKFNMKSKSDIVKLLKPQDELKREESALSLRKNIS
HHCCCCCCCCCCCCEEEEEEEECCCCEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCC
DEDTGMIIKKIKQLRAKKD
CCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA