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The map label for this gene is aksA [H]

Identifier: 150399901

GI number: 150399901

Start: 1173465

End: 1174625

Strand: Reverse

Name: aksA [H]

Synonym: Mevan_1158

Alternate gene names: 150399901

Gene position: 1174625-1173465 (Counterclockwise)

Preceding gene: 150399902

Following gene: 150399897

Centisome position: 68.29

GC content: 34.54

Gene sequence:

>1161_bases
ATGGATTGGAAAGAAGTTTCTCAATATAATCCTAAATTAGACTTAAAAGAATGTTATGTATATGATACTACATTAAGGGA
TGGTGAACAGACTCCTGGAGTTTGTTTTACGGGTAATCAAAAGCTTGAAATTGCTAAAAAATTAGATGACCTTGGAATAA
AACAAATTGAAGCCGGTTTTCCAACGGTTTCTGAAAATGAAAGAAAATGTATTAAATCAATTTCCTCTGAAGGACTTAAT
GCGGATATTTTGGCGCTATCAAGAGTTTTAAAGGAAGATATCGATAGGGCGATTGAATGCGATGTTGATGGAATAATCAC
TTTTGTTGCAACTTCGCCTATGCACCTAAAATATAAGCTACATAAGTCTTTTGAAGAAGTTGAAGAAATGGGAATGAAAG
CTATCGAGTATGCAAAAGATCATGGCCTTTTTGTTGCGTTTTCTGCAGAAGATGCAACAAGAACCTCGATTGAAAATATC
ATAAAAATTCATAAAAATGCAGAAGATTACGGTGCAGATAGGGTCCATATTGCAGATACACTTGGATGTGCAACACCTCA
ATCAATGTACCAAATATGTTCTGAACTGAATAAAAGCCTGAAAAAAGCACATATTGGAGTTCATTGCCATAATGATTTTG
GATTTGCAGCCATAAATTCAATATACGGACTTATGGGTGGTGCAAAGGCAGTTTCAACTACTGTTAATGGCATTGGAGAA
AGGGCCGGAAATGCAGCACTAGAAGAGGTAGTAATGGCTTTAAAAGTACTTTATAATTACGATATGGGGCTAAATACTGA
ACTTATAATGGAAACTTCAAAGTTAGTTGAAACATATTCAAAAATCAAGGTTCCAGAAAATAAGCCTCTTGTTGGCGAAA
TGGTATTTTATCATGAAAGCGGAATACACGTTGATGCAGTTTTAGAAAATCCTTTAACCTATGAGCCGTTTTTACCTGAA
AAAATCGGTCAAAAACGAAAAATTGTGCTTGGAAAACATTCTGGATGCAGAGCCGTTGCATATAGATTAAATGAACTTGG
ATTTGAAGCGACTCGTGATGAACTCTGGGAAATTGTTAAAAAAACTAAGGAAACGAGAGAACAAGGAACCGAAATAAGTG
ACGAAGTATTTAAAAACATTGTAACACATATTTTAAATTAA

Upstream 100 bases:

>100_bases
AATATATACATTTTAGAAGTTAATAAATAATTATTTTAATGTAATATATTATTTTAATATTTTAAAAGTAATTTTCTATT
TTTTATTGGGAGGATTTGTT

Downstream 100 bases:

>100_bases
AATATTTACAATTTTTTTAAAGGTGGGATATTGTTAGAACTTATCGTTACAGGAATAATAGTTGCATTAATTGTATTTTT
AATATTAAAATTCATATTTA

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1 [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MDWKEVSQYNPKLDLKECYVYDTTLRDGEQTPGVCFTGNQKLEIAKKLDDLGIKQIEAGFPTVSENERKCIKSISSEGLN
ADILALSRVLKEDIDRAIECDVDGIITFVATSPMHLKYKLHKSFEEVEEMGMKAIEYAKDHGLFVAFSAEDATRTSIENI
IKIHKNAEDYGADRVHIADTLGCATPQSMYQICSELNKSLKKAHIGVHCHNDFGFAAINSIYGLMGGAKAVSTTVNGIGE
RAGNAALEEVVMALKVLYNYDMGLNTELIMETSKLVETYSKIKVPENKPLVGEMVFYHESGIHVDAVLENPLTYEPFLPE
KIGQKRKIVLGKHSGCRAVAYRLNELGFEATRDELWEIVKKTKETREQGTEISDEVFKNIVTHILN

Sequences:

>Translated_386_residues
MDWKEVSQYNPKLDLKECYVYDTTLRDGEQTPGVCFTGNQKLEIAKKLDDLGIKQIEAGFPTVSENERKCIKSISSEGLN
ADILALSRVLKEDIDRAIECDVDGIITFVATSPMHLKYKLHKSFEEVEEMGMKAIEYAKDHGLFVAFSAEDATRTSIENI
IKIHKNAEDYGADRVHIADTLGCATPQSMYQICSELNKSLKKAHIGVHCHNDFGFAAINSIYGLMGGAKAVSTTVNGIGE
RAGNAALEEVVMALKVLYNYDMGLNTELIMETSKLVETYSKIKVPENKPLVGEMVFYHESGIHVDAVLENPLTYEPFLPE
KIGQKRKIVLGKHSGCRAVAYRLNELGFEATRDELWEIVKKTKETREQGTEISDEVFKNIVTHILN
>Mature_386_residues
MDWKEVSQYNPKLDLKECYVYDTTLRDGEQTPGVCFTGNQKLEIAKKLDDLGIKQIEAGFPTVSENERKCIKSISSEGLN
ADILALSRVLKEDIDRAIECDVDGIITFVATSPMHLKYKLHKSFEEVEEMGMKAIEYAKDHGLFVAFSAEDATRTSIENI
IKIHKNAEDYGADRVHIADTLGCATPQSMYQICSELNKSLKKAHIGVHCHNDFGFAAINSIYGLMGGAKAVSTTVNGIGE
RAGNAALEEVVMALKVLYNYDMGLNTELIMETSKLVETYSKIKVPENKPLVGEMVFYHESGIHVDAVLENPLTYEPFLPE
KIGQKRKIVLGKHSGCRAVAYRLNELGFEATRDELWEIVKKTKETREQGTEISDEVFKNIVTHILN

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=367, Percent_Identity=37.3297002724796, Blast_Score=233, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6320019, Length=364, Percent_Identity=34.3406593406593, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6320071, Length=373, Percent_Identity=33.5120643431635, Blast_Score=206, Evalue=5e-54,
Organism=Drosophila melanogaster, GI24582381, Length=276, Percent_Identity=23.9130434782609, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 43148; Mature: 43148

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWKEVSQYNPKLDLKECYVYDTTLRDGEQTPGVCFTGNQKLEIAKKLDDLGIKQIEAGF
CCCHHHHHCCCCCCHHHEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHCCC
PTVSENERKCIKSISSEGLNADILALSRVLKEDIDRAIECDVDGIITFVATSPMHLKYKL
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEHEEECCCCEEEHHH
HKSFEEVEEMGMKAIEYAKDHGLFVAFSAEDATRTSIENIIKIHKNAEDYGADRVHIADT
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCHHHCCCEEEEHHH
LGCATPQSMYQICSELNKSLKKAHIGVHCHNDFGFAAINSIYGLMGGAKAVSTTVNGIGE
HCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
RAGNAALEEVVMALKVLYNYDMGLNTELIMETSKLVETYSKIKVPENKPLVGEMVFYHES
HCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEECC
GIHVDAVLENPLTYEPFLPEKIGQKRKIVLGKHSGCRAVAYRLNELGFEATRDELWEIVK
CCEEEEEECCCCCCCCCCCHHHCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHH
KTKETREQGTEISDEVFKNIVTHILN
HHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDWKEVSQYNPKLDLKECYVYDTTLRDGEQTPGVCFTGNQKLEIAKKLDDLGIKQIEAGF
CCCHHHHHCCCCCCHHHEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHCCC
PTVSENERKCIKSISSEGLNADILALSRVLKEDIDRAIECDVDGIITFVATSPMHLKYKL
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHEEHEEECCCCEEEHHH
HKSFEEVEEMGMKAIEYAKDHGLFVAFSAEDATRTSIENIIKIHKNAEDYGADRVHIADT
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCHHHCCCEEEEHHH
LGCATPQSMYQICSELNKSLKKAHIGVHCHNDFGFAAINSIYGLMGGAKAVSTTVNGIGE
HCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
RAGNAALEEVVMALKVLYNYDMGLNTELIMETSKLVETYSKIKVPENKPLVGEMVFYHES
HCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEECC
GIHVDAVLENPLTYEPFLPEKIGQKRKIVLGKHSGCRAVAYRLNELGFEATRDELWEIVK
CCEEEEEECCCCCCCCCCCHHHCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHH
KTKETREQGTEISDEVFKNIVTHILN
HHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]