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The map label for this gene is bioB [C]

Identifier: 150399876

GI number: 150399876

Start: 1141761

End: 1142786

Strand: Reverse

Name: bioB [C]

Synonym: Mevan_1131

Alternate gene names: 150399876

Gene position: 1142786-1141761 (Counterclockwise)

Preceding gene: 150399877

Following gene: 150399875

Centisome position: 66.44

GC content: 33.14

Gene sequence:

>1026_bases
ATGATATTTTCAAAGGTAAAAAGCAATTTTCAAGCGCTAAAGGATGGAAAAATAGATTTGGAACAGGGATTAATCTCAAA
AGAAGATGCTTTGGCACTATTTAACATCCGGACTTGGGATGAATGTTTAAAACTTTTTTCGATTGCATCGGAAGTCAGGG
ATTTTTTTAAACCTGAAATTGAAATTACTTCAACAGTTCATGTTACAAACGTCTGTTCAGTAAGTCCTAAATGTAAATAC
TGTGGTTTTGCTGCTGGAACTAGTTCTGAAGGTTATGTTACTGCATTTAAATCAGATGATGACCAAATTAAAAAATCTGT
ACTAGAGATTGAAAAATCAGGAATAGAAAGGGTAAGTTGCTCATCAGGACACGGTTACGGCGGTAAAGAAGTTATTCGTG
CACTTAAAGCCGTTAAATCAGTTTCAAATTTGGAAGTTTTAGTAAATACAGGGGCAGATTTAACTGAAAAATGTGTAATA
GAGCTAAAAGAAAACGGTATTGACACAATATGCTGTAATCTGGAAACCATAAATGAAGAAGTATTTAACAGTGTAAAACC
AGGTGAAAGCCTTTCAGATAGAATACTTGTTTGTAACTTAATTAAAAAACACGGAATTGAATTATCATCAGGTCTTTTAA
TTGGAATTGGTGAATCATACGAAGATAGGGTAGACCATCTTTTTTTCTTAAAAAAACTTGGTGTTGAAGAAATTCCGATA
ATGGGATTTAATCCATACAAAAATACGCCAATGGAAGACTGTAAAAAATGTTCTGCAATTGAACAAGCGAAAACTATCGC
AATTACAAGATTATTATTCCCAAATATTAGGATTACATCTCCAACGCCAACAATTGGCCCAGAACTTTTACAATTTGCCC
TTTTAGGTGGGGCCAGCAATATTGCAACAGTAATTCCAAAAAATCATCCATTAAATGTTATGGGAGTTGGAAGTCCAAAG
ACTGGAAATTTAATTGAAGTAGTCTGCCTAATAAAAGAACTCGGTTTAAAACCTAAAATTAAATAA

Upstream 100 bases:

>100_bases
GCAAAAGAAAACAAATGCGAATGCGGTTGTTCAATCAAACCATAATTCATAATTACCTCTTTTTTTAATATAACTTTAAT
TTACATTTCCGGTGGTCTTA

Downstream 100 bases:

>100_bases
ATTCGCAAAAACAAGGTGGAATATATGAAAGAACTTATAAAAAGTTCCCTAAACGATTTCGATTCTGCAATGGAACTGCG
GGAAATTGTCGTGAAAAAAA

Product: biotin synthase

Products: biotin

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MIFSKVKSNFQALKDGKIDLEQGLISKEDALALFNIRTWDECLKLFSIASEVRDFFKPEIEITSTVHVTNVCSVSPKCKY
CGFAAGTSSEGYVTAFKSDDDQIKKSVLEIEKSGIERVSCSSGHGYGGKEVIRALKAVKSVSNLEVLVNTGADLTEKCVI
ELKENGIDTICCNLETINEEVFNSVKPGESLSDRILVCNLIKKHGIELSSGLLIGIGESYEDRVDHLFFLKKLGVEEIPI
MGFNPYKNTPMEDCKKCSAIEQAKTIAITRLLFPNIRITSPTPTIGPELLQFALLGGASNIATVIPKNHPLNVMGVGSPK
TGNLIEVVCLIKELGLKPKIK

Sequences:

>Translated_341_residues
MIFSKVKSNFQALKDGKIDLEQGLISKEDALALFNIRTWDECLKLFSIASEVRDFFKPEIEITSTVHVTNVCSVSPKCKY
CGFAAGTSSEGYVTAFKSDDDQIKKSVLEIEKSGIERVSCSSGHGYGGKEVIRALKAVKSVSNLEVLVNTGADLTEKCVI
ELKENGIDTICCNLETINEEVFNSVKPGESLSDRILVCNLIKKHGIELSSGLLIGIGESYEDRVDHLFFLKKLGVEEIPI
MGFNPYKNTPMEDCKKCSAIEQAKTIAITRLLFPNIRITSPTPTIGPELLQFALLGGASNIATVIPKNHPLNVMGVGSPK
TGNLIEVVCLIKELGLKPKIK
>Mature_341_residues
MIFSKVKSNFQALKDGKIDLEQGLISKEDALALFNIRTWDECLKLFSIASEVRDFFKPEIEITSTVHVTNVCSVSPKCKY
CGFAAGTSSEGYVTAFKSDDDQIKKSVLEIEKSGIERVSCSSGHGYGGKEVIRALKAVKSVSNLEVLVNTGADLTEKCVI
ELKENGIDTICCNLETINEEVFNSVKPGESLSDRILVCNLIKKHGIELSSGLLIGIGESYEDRVDHLFFLKKLGVEEIPI
MGFNPYKNTPMEDCKKCSAIEQAKTIAITRLLFPNIRITSPTPTIGPELLQFALLGGASNIATVIPKNHPLNVMGVGSPK
TGNLIEVVCLIKELGLKPKIK

Specific function: Biotin biosynthesis; last step. [C]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786992, Length=269, Percent_Identity=23.4200743494424, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: 2.8.1.6

Molecular weight: Translated: 37243; Mature: 37243

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSKVKSNFQALKDGKIDLEQGLISKEDALALFNIRTWDECLKLFSIASEVRDFFKPEI
CCHHHHHHHHHHHHCCCEEHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCE
EITSTVHVTNVCSVSPKCKYCGFAAGTSSEGYVTAFKSDDDQIKKSVLEIEKSGIERVSC
EEEEEEEEEEEECCCCCCCCCEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEC
SSGHGYGGKEVIRALKAVKSVSNLEVLVNTGADLTEKCVIELKENGIDTICCNLETINEE
CCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHCCCCCEEEECHHHHHHH
VFNSVKPGESLSDRILVCNLIKKHGIELSSGLLIGIGESYEDRVDHLFFLKKLGVEEIPI
HHHCCCCCCCHHHHHHHHHHHHHCCCEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCCE
MGFNPYKNTPMEDCKKCSAIEQAKTIAITRLLFPNIRITSPTPTIGPELLQFALLGGASN
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHCCCCC
IATVIPKNHPLNVMGVGSPKTGNLIEVVCLIKELGLKPKIK
EEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIFSKVKSNFQALKDGKIDLEQGLISKEDALALFNIRTWDECLKLFSIASEVRDFFKPEI
CCHHHHHHHHHHHHCCCEEHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCE
EITSTVHVTNVCSVSPKCKYCGFAAGTSSEGYVTAFKSDDDQIKKSVLEIEKSGIERVSC
EEEEEEEEEEEECCCCCCCCCEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEC
SSGHGYGGKEVIRALKAVKSVSNLEVLVNTGADLTEKCVIELKENGIDTICCNLETINEE
CCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHHHCCCCCEEEECHHHHHHH
VFNSVKPGESLSDRILVCNLIKKHGIELSSGLLIGIGESYEDRVDHLFFLKKLGVEEIPI
HHHCCCCCCCHHHHHHHHHHHHHCCCEECCCEEEEECCCHHHHHHHHHHHHHCCCCCCCE
MGFNPYKNTPMEDCKKCSAIEQAKTIAITRLLFPNIRITSPTPTIGPELLQFALLGGASN
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHCCCCC
IATVIPKNHPLNVMGVGSPKTGNLIEVVCLIKELGLKPKIK
EEEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.002 {dethiobiotin}} [C]

Substrates: dethiobiotin; sulfur

Specific reaction: dethiobiotin + sulfur = biotin

General reaction: Sulfur atom transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]