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The map label for this gene is ynfL [C]

Identifier: 150399762

GI number: 150399762

Start: 1026326

End: 1027216

Strand: Reverse

Name: ynfL [C]

Synonym: Mevan_1014

Alternate gene names: 150399762

Gene position: 1027216-1026326 (Counterclockwise)

Preceding gene: 150399764

Following gene: 150399761

Centisome position: 59.72

GC content: 33.56

Gene sequence:

>891_bases
ATGGATCCAAAAATTAGTTATTTTCAAACATTTATTTACGCCTCAAAGACTAAAAGCTTTTCTAAGGCTGCAAAAAAACT
TGGTGTTACCCAAGGAACAGTAAGTAACCACATATCTGTTTTAGAAAAGTATTTTGATGCACAACTATTTTTAAGAACTC
CTGAAGGTGTAGATTTAACGCTTGAAGGAAACATCCTTTATGAAAATTCCGAAAAAATACTTGAAAGTATAAACAACTCA
AAGCAACAGATGAGAATACTCCATGAACATCCTGAAGGAGTAATAAAAATTGCAGCAAGTACTACACCTGGAGAACACCT
TCTTCCAAGTATACTTAAAGACTATACTAAAGAATATAAGGATGTTTCTTTTCAAATACAGATTACTGATTCTGAAAAGT
GTTTTAAACTTTTAGAGGATAGGGTAGTTGATATAATTGCAGTAGGGAATATTTACGATAAAACATATGAAAGTATAGTA
ATCGGAAAAGACAGGCTTGTATTAATCGTTCCATCAGAACACCCTCTTTCAAAAAAATCGGTTGCAAAACTTGCAGACGT
TTTAAAAGAAGACTATATTGATAGAGAAATAGGTTCGGGAACTAGAGAAATTTTGGTAGATGCATTACAAGAAAAAGGCT
ATTCAATGATGGATTTACACATAGTAATGAGCCTTGGAAGTACTTCTTCAATAATTACCGCAGTTTCCGAAGGTTATGGG
GTAAGTATTCTTTCAGAAATTCCTGCAAGAAAAGCAGCTGAATCCGGGCAATTAAAGATAGTGCCGATTGAAGATTTAGA
CCTTACAAGATACCTATACCTTGTAAAGGGTAAAAAACCAAGAAATCCAAGTGCAATAAAGTCTTTTTGGGACTTTGTTT
CTGGCCAGTAA

Upstream 100 bases:

>100_bases
TTATATGATTTTAAGAATAGTATTCGAATGATATATATATTAGTTTTTCTAATAAGTATATAGTATCTTAAGTTAAGAAT
TAAGGCAAATGTGATAGATT

Downstream 100 bases:

>100_bases
TTTTGAATTTTTATAAAAATCACTTAAAAAACCATATATATTTTTGTACAAAATAGGTTTGTAATAATTCATACAGATAA
AACTAACTGGTGAATAGATG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MDPKISYFQTFIYASKTKSFSKAAKKLGVTQGTVSNHISVLEKYFDAQLFLRTPEGVDLTLEGNILYENSEKILESINNS
KQQMRILHEHPEGVIKIAASTTPGEHLLPSILKDYTKEYKDVSFQIQITDSEKCFKLLEDRVVDIIAVGNIYDKTYESIV
IGKDRLVLIVPSEHPLSKKSVAKLADVLKEDYIDREIGSGTREILVDALQEKGYSMMDLHIVMSLGSTSSIITAVSEGYG
VSILSEIPARKAAESGQLKIVPIEDLDLTRYLYLVKGKKPRNPSAIKSFWDFVSGQ

Sequences:

>Translated_296_residues
MDPKISYFQTFIYASKTKSFSKAAKKLGVTQGTVSNHISVLEKYFDAQLFLRTPEGVDLTLEGNILYENSEKILESINNS
KQQMRILHEHPEGVIKIAASTTPGEHLLPSILKDYTKEYKDVSFQIQITDSEKCFKLLEDRVVDIIAVGNIYDKTYESIV
IGKDRLVLIVPSEHPLSKKSVAKLADVLKEDYIDREIGSGTREILVDALQEKGYSMMDLHIVMSLGSTSSIITAVSEGYG
VSILSEIPARKAAESGQLKIVPIEDLDLTRYLYLVKGKKPRNPSAIKSFWDFVSGQ
>Mature_296_residues
MDPKISYFQTFIYASKTKSFSKAAKKLGVTQGTVSNHISVLEKYFDAQLFLRTPEGVDLTLEGNILYENSEKILESINNS
KQQMRILHEHPEGVIKIAASTTPGEHLLPSILKDYTKEYKDVSFQIQITDSEKCFKLLEDRVVDIIAVGNIYDKTYESIV
IGKDRLVLIVPSEHPLSKKSVAKLADVLKEDYIDREIGSGTREILVDALQEKGYSMMDLHIVMSLGSTSSIITAVSEGYG
VSILSEIPARKAAESGQLKIVPIEDLDLTRYLYLVKGKKPRNPSAIKSFWDFVSGQ

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=294, Percent_Identity=25.8503401360544, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788481, Length=297, Percent_Identity=27.2727272727273, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788748, Length=302, Percent_Identity=24.5033112582781, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI145693105, Length=279, Percent_Identity=22.2222222222222, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI157672245, Length=284, Percent_Identity=22.5352112676056, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081904, Length=210, Percent_Identity=25.2380952380952, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787128, Length=149, Percent_Identity=30.2013422818792, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI2367136, Length=238, Percent_Identity=22.6890756302521, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789173, Length=282, Percent_Identity=21.9858156028369, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789204, Length=255, Percent_Identity=23.5294117647059, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789440, Length=260, Percent_Identity=24.6153846153846, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788296, Length=273, Percent_Identity=24.5421245421245, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786401, Length=148, Percent_Identity=26.3513513513513, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87081978, Length=147, Percent_Identity=25.1700680272109, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33097; Mature: 33097

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPKISYFQTFIYASKTKSFSKAAKKLGVTQGTVSNHISVLEKYFDAQLFLRTPEGVDLT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEECCCCEEEE
LEGNILYENSEKILESINNSKQQMRILHEHPEGVIKIAASTTPGEHLLPSILKDYTKEYK
EECCEEEECHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
DVSFQIQITDSEKCFKLLEDRVVDIIAVGNIYDKTYESIVIGKDRLVLIVPSEHPLSKKS
CEEEEEEECCHHHHHHHHHHHHEEEEEECCHHHHHHHEEEECCCEEEEEECCCCCCCHHH
VAKLADVLKEDYIDREIGSGTREILVDALQEKGYSMMDLHIVMSLGSTSSIITAVSEGYG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHCCCC
VSILSEIPARKAAESGQLKIVPIEDLDLTRYLYLVKGKKPRNPSAIKSFWDFVSGQ
CHHHHHCCHHHCCCCCCEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MDPKISYFQTFIYASKTKSFSKAAKKLGVTQGTVSNHISVLEKYFDAQLFLRTPEGVDLT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEECCCCEEEE
LEGNILYENSEKILESINNSKQQMRILHEHPEGVIKIAASTTPGEHLLPSILKDYTKEYK
EECCEEEECHHHHHHHHCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
DVSFQIQITDSEKCFKLLEDRVVDIIAVGNIYDKTYESIVIGKDRLVLIVPSEHPLSKKS
CEEEEEEECCHHHHHHHHHHHHEEEEEECCHHHHHHHEEEECCCEEEEEECCCCCCCHHH
VAKLADVLKEDYIDREIGSGTREILVDALQEKGYSMMDLHIVMSLGSTSSIITAVSEGYG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHCCCC
VSILSEIPARKAAESGQLKIVPIEDLDLTRYLYLVKGKKPRNPSAIKSFWDFVSGQ
CHHHHHCCHHHCCCCCCEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]