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The map label for this gene is eif2a [H]

Identifier: 150399757

GI number: 150399757

Start: 1023490

End: 1024287

Strand: Reverse

Name: eif2a [H]

Synonym: Mevan_1009

Alternate gene names: 150399757

Gene position: 1024287-1023490 (Counterclockwise)

Preceding gene: 150399758

Following gene: 150399756

Centisome position: 59.55

GC content: 36.72

Gene sequence:

>798_bases
ATGCGGAAAGATTTTCCTGAAGAGGGAGATATTGTTATTGGAAATGTTACTGATGTGAAAGCATTTGGTGCATTTATCGA
GCTTTTAGAGTACCCTGGAAAAGAGGGAATGGTTCATATTTCTGAAGTGTCTTCAGGATGGATTAAAAATATACGAGACC
ACATTAAAAAAGGTCAAAGAGTAGTTGCAAAGGTAATGAGGGTAAATCCCGGTAAAAACCAGATAGACCTTTCTTTAAAA
CGTGCAACTGACCAACAAAAAAAATCTAAGGTTCAAGAATGGAAAAAATTCCAAAGAGCTGAAAAATTACTCCAATTTGC
ATCTGAAAAGCTTGGAAAAACCCTTGAAGATGGATGGGAAGCTGTAGGTTACTTGTTAGTTGATAAATTCGGTGAACTTT
ACGATGCATTTGAATCAATGGTAATTGAAGGAAAAGAAGTATTAGATGAACTCGAAACTCCGGTTTCTAAAGAATGGGCA
GATATGATTTATGAAGTTGCATGTGAAAACATCGAACTTTCAAACGTTAAAGTCGATGGAATTCTTACACTTACAACCAC
GGAACCTTCAGGGGTAAAAGTAATTAAAAACGGTCTTAAAAAGGCTTTCAAAGCAAACCCTTACGAAGACGTTAAGGTGA
ATATTACGTATGTCGGGGCTCCAAAATACCGGATAGAAGTAATTGCACCAGATTATAAGAGTGGTGAAGAAGTTTTAAGA
AGAGTTGCAGAAGAAGCAATTGAGTATATTAAAGACTACAAGGGCGGACAGGGTAGTTTTGTTAGGGTAGAGGAGTAA

Upstream 100 bases:

>100_bases
AAATTTTTAAAATAGCTTGAATAGTTTTATAAGTATTTAAATACTATATTGTGTATAAACTAGTCAGTATGCTATTTATA
ATTTAATCAAGGTGGAACTT

Downstream 100 bases:

>100_bases
TCGATACCATGCAGATGAAAAAATGTCCAAAATGTGGCAAATATACTTTAAAAGAGTACTGTATTGACTGTAATGAAAAA
GCAGGTACTGTCAAACCTCC

Product: translation initiation factor IF-2 subunit alpha

Products: RNAn; a nucleoside diphosphate [C]

Alternate protein names: aIF2-alpha; eIF-2-alpha [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MRKDFPEEGDIVIGNVTDVKAFGAFIELLEYPGKEGMVHISEVSSGWIKNIRDHIKKGQRVVAKVMRVNPGKNQIDLSLK
RATDQQKKSKVQEWKKFQRAEKLLQFASEKLGKTLEDGWEAVGYLLVDKFGELYDAFESMVIEGKEVLDELETPVSKEWA
DMIYEVACENIELSNVKVDGILTLTTTEPSGVKVIKNGLKKAFKANPYEDVKVNITYVGAPKYRIEVIAPDYKSGEEVLR
RVAEEAIEYIKDYKGGQGSFVRVEE

Sequences:

>Translated_265_residues
MRKDFPEEGDIVIGNVTDVKAFGAFIELLEYPGKEGMVHISEVSSGWIKNIRDHIKKGQRVVAKVMRVNPGKNQIDLSLK
RATDQQKKSKVQEWKKFQRAEKLLQFASEKLGKTLEDGWEAVGYLLVDKFGELYDAFESMVIEGKEVLDELETPVSKEWA
DMIYEVACENIELSNVKVDGILTLTTTEPSGVKVIKNGLKKAFKANPYEDVKVNITYVGAPKYRIEVIAPDYKSGEEVLR
RVAEEAIEYIKDYKGGQGSFVRVEE
>Mature_265_residues
MRKDFPEEGDIVIGNVTDVKAFGAFIELLEYPGKEGMVHISEVSSGWIKNIRDHIKKGQRVVAKVMRVNPGKNQIDLSLK
RATDQQKKSKVQEWKKFQRAEKLLQFASEKLGKTLEDGWEAVGYLLVDKFGELYDAFESMVIEGKEVLDELETPVSKEWA
DMIYEVACENIELSNVKVDGILTLTTTEPSGVKVIKNGLKKAFKANPYEDVKVNITYVGAPKYRIEVIAPDYKSGEEVLR
RVAEEAIEYIKDYKGGQGSFVRVEE

Specific function: eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA [H]

COG id: COG1093

COG function: function code J; Translation initiation factor 2, alpha subunit (eIF-2alpha)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI4758256, Length=259, Percent_Identity=24.7104247104247, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17509871, Length=258, Percent_Identity=24.8062015503876, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6322466, Length=261, Percent_Identity=30.6513409961686, Blast_Score=113, Evalue=3e-26,
Organism=Drosophila melanogaster, GI18860087, Length=259, Percent_Identity=27.027027027027, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011488
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR022964 [H]

Pfam domain/function: PF07541 EIF_2_alpha; PF00575 S1 [H]

EC number: 2.7.7.8 [C]

Molecular weight: Translated: 29986; Mature: 29986

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKDFPEEGDIVIGNVTDVKAFGAFIELLEYPGKEGMVHISEVSSGWIKNIRDHIKKGQR
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
VVAKVMRVNPGKNQIDLSLKRATDQQKKSKVQEWKKFQRAEKLLQFASEKLGKTLEDGWE
HHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AVGYLLVDKFGELYDAFESMVIEGKEVLDELETPVSKEWADMIYEVACENIELSNVKVDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCEEEEE
ILTLTTTEPSGVKVIKNGLKKAFKANPYEDVKVNITYVGAPKYRIEVIAPDYKSGEEVLR
EEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCEEEEEECCCCCCHHHHHH
RVAEEAIEYIKDYKGGQGSFVRVEE
HHHHHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure
MRKDFPEEGDIVIGNVTDVKAFGAFIELLEYPGKEGMVHISEVSSGWIKNIRDHIKKGQR
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
VVAKVMRVNPGKNQIDLSLKRATDQQKKSKVQEWKKFQRAEKLLQFASEKLGKTLEDGWE
HHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AVGYLLVDKFGELYDAFESMVIEGKEVLDELETPVSKEWADMIYEVACENIELSNVKVDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEECCEEEEE
ILTLTTTEPSGVKVIKNGLKKAFKANPYEDVKVNITYVGAPKYRIEVIAPDYKSGEEVLR
EEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCEEEEEECCCCCCHHHHHH
RVAEEAIEYIKDYKGGQGSFVRVEE
HHHHHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 4500 [C]

Specific activity: NA

Km value (mM): NA

Substrates: RNAn+1; phosphate [C]

Specific reaction: RNAn+1 + phosphate = RNAn + a nucleoside diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]