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The map label for this gene is vhuA [H]

Identifier: 150399744

GI number: 150399744

Start: 1011195

End: 1012451

Strand: Reverse

Name: vhuA [H]

Synonym: Mevan_0996

Alternate gene names: 150399744

Gene position: 1012451-1011195 (Counterclockwise)

Preceding gene: 150399745

Following gene: 150399743

Centisome position: 58.86

GC content: 41.77

Gene sequence:

>1257_bases
ATGGGTAAGGTTACAATCGAACCTCTAACCCGTTTAGAAGGGCACGGTAAGGTTACTATAACCTTAGACGATTCCGGAAA
ACCAACGGATGTAAAGTTAAACATTACTGCCCTTAGAGGTTTCGAGCAATTCGTTGTAGGAAGACCTGCAGAGGAAGTAC
CAAGGATTGTTCCAAGAATTTGTGGAATTTGTCAGACGGCTCACCACTTAGCGAGTGTTAAAGCAGTAGATGCAGCGTGG
GGGGCACAAATCCCAATCGCAGCGGAAAAATTGAGAGAATTAATGCACTTGGGTAACATGATTCACTCACACGCTTTACA
CTTTTACTACTTGGCAGCTCCTGACTTTGTACTTGGCCCCGATGCAGACCCTGCAGTAAGAAATATTATTGGAGTTATTG
GCGCAGCTCCAGAAGTTGCTAAAAAAGCAATCGCAATGAGAAGATTTGGACAAACGATCGTTGAAGCCGTAGGTGGGAGA
CCAATTCACCCAGTAACTGGTATTCCTGGCGGACAAGCTAAGGTTTTCACCGAAGAAAAAAGGGACGAAATCCTAACTGA
AATGGATGCAATGATTGAATTTGGCCAAGAAGGACTCGCTATAATGAAGTCCCTCAACGAAAAATACTTAGATACAATAA
AAACACTTGGTGTAATCGATACCTGGTATTTAGGTTTAGTAAAAGATGGTAAACACAATTTCTACGGCGACACGTTAAGA
TTCATGTCACCTGACGGAAAAGAAACCATGGATTTCAAACCTTCAGAATATACTGATTACCTTGGTGAACACACTGTAGA
CCATAGCTATGTAAAGTATCCGTACAATAAAAAAGTAGGGTATCCTGATGGTCTTTATAGGGTAGGTCCACTTGCAATGA
TTAACGTATGTGACTCAATGGCTACGCCACTTGCTGAGGAAGCTAGGAAAGAATTTGTAGAAACTTTTGGAAAGCCTGCA
AACCAGTCCTTAGCATACAACCAAGCAAGACTTATAGAGTTACTAAGTGCATGCGAAAGAACCAAACAACTATTGGAAGA
CCCAGAAATTACCTCAACCGACATCAAAGCAGAGGTTCAACCAAAAGCAGGAAATGGTGTCGGAGTAGTATATGCTCCAA
GAGGTACGTTATTCCACAATTATGAAACTGATGACAACGGAATTGTTGTAAAGGCAAACATGATTGTAGCAACGACACAC
AATGTACCTACAATGGAAAAAGCAATCCAGCAGGCTGCAAGTGTGGTCTTCAAATAA

Upstream 100 bases:

>100_bases
GCTACTTAACAGGTTTTCTTTACCTGCAGCATTAATTTCTAAAAAAGTTCAGAAATAAGTATCTTAATTATTAAAAATTG
AGATTAAGGATGGTGACTCT

Downstream 100 bases:

>100_bases
TTGAAGTTCCTCTTGGTGGTAACAATGGTAGATGAAACAAAATTGAATTTGATCGAAATTGTATTAAGGGCGTATGACCC
ATGATACTCATGTGCAGCGC

Product: nickel-dependent hydrogenase large subunit

Products: reduced coenzyme F420

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MGKVTIEPLTRLEGHGKVTITLDDSGKPTDVKLNITALRGFEQFVVGRPAEEVPRIVPRICGICQTAHHLASVKAVDAAW
GAQIPIAAEKLRELMHLGNMIHSHALHFYYLAAPDFVLGPDADPAVRNIIGVIGAAPEVAKKAIAMRRFGQTIVEAVGGR
PIHPVTGIPGGQAKVFTEEKRDEILTEMDAMIEFGQEGLAIMKSLNEKYLDTIKTLGVIDTWYLGLVKDGKHNFYGDTLR
FMSPDGKETMDFKPSEYTDYLGEHTVDHSYVKYPYNKKVGYPDGLYRVGPLAMINVCDSMATPLAEEARKEFVETFGKPA
NQSLAYNQARLIELLSACERTKQLLEDPEITSTDIKAEVQPKAGNGVGVVYAPRGTLFHNYETDDNGIVVKANMIVATTH
NVPTMEKAIQQAASVVFK

Sequences:

>Translated_418_residues
MGKVTIEPLTRLEGHGKVTITLDDSGKPTDVKLNITALRGFEQFVVGRPAEEVPRIVPRICGICQTAHHLASVKAVDAAW
GAQIPIAAEKLRELMHLGNMIHSHALHFYYLAAPDFVLGPDADPAVRNIIGVIGAAPEVAKKAIAMRRFGQTIVEAVGGR
PIHPVTGIPGGQAKVFTEEKRDEILTEMDAMIEFGQEGLAIMKSLNEKYLDTIKTLGVIDTWYLGLVKDGKHNFYGDTLR
FMSPDGKETMDFKPSEYTDYLGEHTVDHSYVKYPYNKKVGYPDGLYRVGPLAMINVCDSMATPLAEEARKEFVETFGKPA
NQSLAYNQARLIELLSACERTKQLLEDPEITSTDIKAEVQPKAGNGVGVVYAPRGTLFHNYETDDNGIVVKANMIVATTH
NVPTMEKAIQQAASVVFK
>Mature_417_residues
GKVTIEPLTRLEGHGKVTITLDDSGKPTDVKLNITALRGFEQFVVGRPAEEVPRIVPRICGICQTAHHLASVKAVDAAWG
AQIPIAAEKLRELMHLGNMIHSHALHFYYLAAPDFVLGPDADPAVRNIIGVIGAAPEVAKKAIAMRRFGQTIVEAVGGRP
IHPVTGIPGGQAKVFTEEKRDEILTEMDAMIEFGQEGLAIMKSLNEKYLDTIKTLGVIDTWYLGLVKDGKHNFYGDTLRF
MSPDGKETMDFKPSEYTDYLGEHTVDHSYVKYPYNKKVGYPDGLYRVGPLAMINVCDSMATPLAEEARKEFVETFGKPAN
QSLAYNQARLIELLSACERTKQLLEDPEITSTDIKAEVQPKAGNGVGVVYAPRGTLFHNYETDDNGIVVKANMIVATTHN
VPTMEKAIQQAASVVFK

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=115, Percent_Identity=35.6521739130435, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1787207, Length=115, Percent_Identity=34.7826086956522, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: 1.12.98.1

Molecular weight: Translated: 45892; Mature: 45761

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVTIEPLTRLEGHGKVTITLDDSGKPTDVKLNITALRGFEQFVVGRPAEEVPRIVPRI
CCCEEECHHHHCCCCCEEEEEECCCCCCCEEEEEEHHHHCHHHHHCCCCHHHHHHHHHHH
CGICQTAHHLASVKAVDAAWGAQIPIAAEKLRELMHLGNMIHSHALHFYYLAAPDFVLGP
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEECC
DADPAVRNIIGVIGAAPEVAKKAIAMRRFGQTIVEAVGGRPIHPVTGIPGGQAKVFTEEK
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHH
RDEILTEMDAMIEFGQEGLAIMKSLNEKYLDTIKTLGVIDTWYLGLVKDGKHNFYGDTLR
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCE
FMSPDGKETMDFKPSEYTDYLGEHTVDHSYVKYPYNKKVGYPDGLYRVGPLAMINVCDSM
EECCCCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHCHHHHHHHHHHH
ATPLAEEARKEFVETFGKPANQSLAYNQARLIELLSACERTKQLLEDPEITSTDIKAEVQ
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEC
PKAGNGVGVVYAPRGTLFHNYETDDNGIVVKANMIVATTHNVPTMEKAIQQAASVVFK
CCCCCCEEEEECCCCCEEECCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GKVTIEPLTRLEGHGKVTITLDDSGKPTDVKLNITALRGFEQFVVGRPAEEVPRIVPRI
CCEEECHHHHCCCCCEEEEEECCCCCCCEEEEEEHHHHCHHHHHCCCCHHHHHHHHHHH
CGICQTAHHLASVKAVDAAWGAQIPIAAEKLRELMHLGNMIHSHALHFYYLAAPDFVLGP
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEECC
DADPAVRNIIGVIGAAPEVAKKAIAMRRFGQTIVEAVGGRPIHPVTGIPGGQAKVFTEEK
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHH
RDEILTEMDAMIEFGQEGLAIMKSLNEKYLDTIKTLGVIDTWYLGLVKDGKHNFYGDTLR
HHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCE
FMSPDGKETMDFKPSEYTDYLGEHTVDHSYVKYPYNKKVGYPDGLYRVGPLAMINVCDSM
EECCCCCCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHCHHHHHHHHHHH
ATPLAEEARKEFVETFGKPANQSLAYNQARLIELLSACERTKQLLEDPEITSTDIKAEVQ
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEC
PKAGNGVGVVYAPRGTLFHNYETDDNGIVVKANMIVATTHNVPTMEKAIQQAASVVFK
CCCCCCEEEEECCCCCEEECCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2; coenzyme F420

Specific reaction: H2 + coenzyme F420 = reduced coenzyme F420

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1603063 [H]