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The map label for this gene is thyA [H]

Identifier: 150399440

GI number: 150399440

Start: 739420

End: 740058

Strand: Reverse

Name: thyA [H]

Synonym: Mevan_0689

Alternate gene names: 150399440

Gene position: 740058-739420 (Counterclockwise)

Preceding gene: 150399449

Following gene: 150399434

Centisome position: 43.03

GC content: 29.26

Gene sequence:

>639_bases
ATGTTACTCTTAAAAAAACAGACAGTTAAATCTGCATTTGAAGAAATGATTCCAAGAATAATGAAATATGGTACTGATAT
GGTTACCGAAGATAATCAAAAATGTAAAGAAATAATGAATATGGTAATTGAAATTACAAATCCAAAAGACAAATCCATAT
CTAAATTGTATCCGCTTGGGGAACGGGCAGTTAAATCATATACTGAACAGTTGATAAATGGATGTACCGCAACTTTTGTT
TATGATTACCATGAAAGAATTTTCAAATATCCAAACGATTTAAAGAATAGAAAATTCGATCAAATTGAATATATTGTAAA
CAAATTAAAAAGTGAGAAAAATTCAAGGCGAGCAGTTTCAATTACGTGGAACCCATATATTGATACTGAAGTAAAGGACG
TTCCATGTTTACAATTTGTACAGTTTCTTATACGGGATAATAAACTTTATCAGACGGTTCTTTTCAGGTCAAATGATGCG
TTTTTAGCATTCCACGCAAATGCAATAGGGTTAATTAGTCTTGGAGAATTAATTGCAGAAAAATTAGGAATTGAATTAGT
AAGATATACTCATCATTCAGTTTCAATGCATGTTTATTTCGAAAGGGATGCAGATAATTTGAAAAAATTTAATTTTTAA

Upstream 100 bases:

>100_bases
TTTACAACAATTACTAAAAAAATTGTAAATCATAATTGTAAATCATAATATTAAAATATCATTTTAATTATCATATTATT
AATCAGTTGGTGAAAAAGCA

Downstream 100 bases:

>100_bases
TGGTTTTTTAAAAATTAATAAATTTTTATTCTTTATTAGATACTTCCTTTAACTTCTTTTTTAATAATAGGTATTCTTTA
CAGTCTTCGATACTTTTAAA

Product: thymidylate synthase

Products: dihydrofolate; dTMP

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MLLLKKQTVKSAFEEMIPRIMKYGTDMVTEDNQKCKEIMNMVIEITNPKDKSISKLYPLGERAVKSYTEQLINGCTATFV
YDYHERIFKYPNDLKNRKFDQIEYIVNKLKSEKNSRRAVSITWNPYIDTEVKDVPCLQFVQFLIRDNKLYQTVLFRSNDA
FLAFHANAIGLISLGELIAEKLGIELVRYTHHSVSMHVYFERDADNLKKFNF

Sequences:

>Translated_212_residues
MLLLKKQTVKSAFEEMIPRIMKYGTDMVTEDNQKCKEIMNMVIEITNPKDKSISKLYPLGERAVKSYTEQLINGCTATFV
YDYHERIFKYPNDLKNRKFDQIEYIVNKLKSEKNSRRAVSITWNPYIDTEVKDVPCLQFVQFLIRDNKLYQTVLFRSNDA
FLAFHANAIGLISLGELIAEKLGIELVRYTHHSVSMHVYFERDADNLKKFNF
>Mature_212_residues
MLLLKKQTVKSAFEEMIPRIMKYGTDMVTEDNQKCKEIMNMVIEITNPKDKSISKLYPLGERAVKSYTEQLINGCTATFV
YDYHERIFKYPNDLKNRKFDQIEYIVNKLKSEKNSRRAVSITWNPYIDTEVKDVPCLQFVQFLIRDNKLYQTVLFRSNDA
FLAFHANAIGLISLGELIAEKLGIELVRYTHHSVSMHVYFERDADNLKKFNF

Specific function: May catalyze the biosynthesis of dTMP using an unknown cosubstrate [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Archaeal- type thyA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR014620
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: 2.1.1.45

Molecular weight: Translated: 24912; Mature: 24912

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLLKKQTVKSAFEEMIPRIMKYGTDMVTEDNQKCKEIMNMVIEITNPKDKSISKLYPLG
CCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEECCCCCCCHHHHHCCCH
ERAVKSYTEQLINGCTATFVYDYHERIFKYPNDLKNRKFDQIEYIVNKLKSEKNSRRAVS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHCCCCCCEEEE
ITWNPYIDTEVKDVPCLQFVQFLIRDNKLYQTVLFRSNDAFLAFHANAIGLISLGELIAE
EEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHEEECCCEEEEEECCCHHHHHHHHHHHH
KLGIELVRYTHHSVSMHVYFERDADNLKKFNF
HHHHHHHEEECCEEEEEEEEECCCHHHHCCCC
>Mature Secondary Structure
MLLLKKQTVKSAFEEMIPRIMKYGTDMVTEDNQKCKEIMNMVIEITNPKDKSISKLYPLG
CCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEECCCCCCCHHHHHCCCH
ERAVKSYTEQLINGCTATFVYDYHERIFKYPNDLKNRKFDQIEYIVNKLKSEKNSRRAVS
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHHHCCCCCCEEEE
ITWNPYIDTEVKDVPCLQFVQFLIRDNKLYQTVLFRSNDAFLAFHANAIGLISLGELIAE
EEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHEEECCCEEEEEECCCHHHHHHHHHHHH
KLGIELVRYTHHSVSMHVYFERDADNLKKFNF
HHHHHHHEEECCEEEEEEEEECCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Dithiothreitol [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,10-methylenetetrahydrofolate; dUMP

Specific reaction: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP

General reaction: Methyl group transfer [C]

Inhibitor: 5'- monophosphate; 10-Formyl dihydrofolyl heptaglutamate; 10-Formyl dihydrofolyl monoglutamate; 10-Formyl tetrahydrofolyl heptaglutamate; 10-Formyl tetrahydrofolyl monoglutamate; Amethopterin; Dithiothreitol [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]