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The map label for this gene is purP

Identifier: 150399434

GI number: 150399434

Start: 734654

End: 735739

Strand: Reverse

Name: purP

Synonym: Mevan_0683

Alternate gene names: 150399434

Gene position: 735739-734654 (Counterclockwise)

Preceding gene: 150399440

Following gene: 150399420

Centisome position: 42.77

GC content: 37.75

Gene sequence:

>1086_bases
ATGATTCCAAAAGAAGAAATAATGGGGATTTTTGAGAAATACAACAAGGATGAGATAACTATCGCAACTGTTGGGAGCCA
TACCTCCTTACACATCCTAAAGGGAGCAAAGTTGGAAGGTTTTTCAACTGCCGTAATTACTACTAAAGATAGAGATGTTC
CTTACAAAAGATTTGGAGTAGCAGACAAATTCATATATGTTGATAAATTTTCCGATATTTCAAGCGAAGAAATTCAAGAA
CAACTACGGGAAATGAACGCAATTGTTGTTCCACACGGTTCATTTATTGCTTATTGCGGGTTAGACAATGTGGAAAGTAC
ATTTAAAGTTCCAATGTTTGGCAATAGGGCCATTTTAAGGTGGGAAGCGGAAAGAGACCTCGAAGGGCAACTTTTAGGCG
GCAGTGGTCTTAGACTTCCAAAAAAATATAACAGTCCAGATGAAATTGATGGGCCAGTAATGGTTAAATTCCCAGGTGCA
AGGGGGGGTAGAGGATACTTCCCATGTTCTTCAACTGAAGAATTCTGGAGAAAAATTAACGATTTCAAGGCTAAAGGCGT
TCTTTCTGAAGAAGATGTTAAAAAGGCACATATTGAAGAATACGTAGTTGGTGCAAACTACTGTATTCATTATTTCTATT
CGCCATTAAAGGATAGGGTAGAACTAATGGGTATTGATAGAAGATACGAAAGCAGTATTGATGGACTTGTTAGGGTTCCT
TCAAAAGACCAGTTAGAATTGGATATTGACCCTTCATACGTTATCACTGGAAATTTCCCAGTAGTCATAAGGGAAAGTCT
GCTTCCACAAGTGTTTGATATGGGGGACAAGTTATGTAAAAAGGCAGAAGAACTTGTAAAACCTGGAATGCTTGGGCCGT
TTTGCTTACAGTCACTATGTACTGAAAACTTAGAACTCGTTGTATTTGAGATGAGTGCAAGATCAGACGGTGGAAACAAC
ACCTTTATGAATGGAAGCCCATATTCATATTTATATAATGCTGAACCACTAAGCATGGGTCAAAGAATTGCAAAAGAAAT
CAAATTGGCTCTTGAGCTCAAAATGATAGAAAAAGTTATATCCTAA

Upstream 100 bases:

>100_bases
TTGACTGGAAACGTATAAAAAATAGTAACACGAATATACCTATGCTTGAATATTGTACATACTACTCTATTTTAAATACC
CTATAGACCATGGTGGTAAA

Downstream 100 bases:

>100_bases
CTTATTTATTTTTTATTCAGCATTAAAATAGGGTATTTAAATTTATTTAAGTATATTTAGGTTTATTTAAATTGAATTTA
CAGATAAAAAAAGTTTAAGA

Product: 5-formaminoimidazole-4-carboxamide-1-(beta)-D- ribofuranosyl 5'-monophosphate synthetase

Products: NA

Alternate protein names: 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MIPKEEIMGIFEKYNKDEITIATVGSHTSLHILKGAKLEGFSTAVITTKDRDVPYKRFGVADKFIYVDKFSDISSEEIQE
QLREMNAIVVPHGSFIAYCGLDNVESTFKVPMFGNRAILRWEAERDLEGQLLGGSGLRLPKKYNSPDEIDGPVMVKFPGA
RGGRGYFPCSSTEEFWRKINDFKAKGVLSEEDVKKAHIEEYVVGANYCIHYFYSPLKDRVELMGIDRRYESSIDGLVRVP
SKDQLELDIDPSYVITGNFPVVIRESLLPQVFDMGDKLCKKAEELVKPGMLGPFCLQSLCTENLELVVFEMSARSDGGNN
TFMNGSPYSYLYNAEPLSMGQRIAKEIKLALELKMIEKVIS

Sequences:

>Translated_361_residues
MIPKEEIMGIFEKYNKDEITIATVGSHTSLHILKGAKLEGFSTAVITTKDRDVPYKRFGVADKFIYVDKFSDISSEEIQE
QLREMNAIVVPHGSFIAYCGLDNVESTFKVPMFGNRAILRWEAERDLEGQLLGGSGLRLPKKYNSPDEIDGPVMVKFPGA
RGGRGYFPCSSTEEFWRKINDFKAKGVLSEEDVKKAHIEEYVVGANYCIHYFYSPLKDRVELMGIDRRYESSIDGLVRVP
SKDQLELDIDPSYVITGNFPVVIRESLLPQVFDMGDKLCKKAEELVKPGMLGPFCLQSLCTENLELVVFEMSARSDGGNN
TFMNGSPYSYLYNAEPLSMGQRIAKEIKLALELKMIEKVIS
>Mature_361_residues
MIPKEEIMGIFEKYNKDEITIATVGSHTSLHILKGAKLEGFSTAVITTKDRDVPYKRFGVADKFIYVDKFSDISSEEIQE
QLREMNAIVVPHGSFIAYCGLDNVESTFKVPMFGNRAILRWEAERDLEGQLLGGSGLRLPKKYNSPDEIDGPVMVKFPGA
RGGRGYFPCSSTEEFWRKINDFKAKGVLSEEDVKKAHIEEYVVGANYCIHYFYSPLKDRVELMGIDRRYESSIDGLVRVP
SKDQLELDIDPSYVITGNFPVVIRESLLPQVFDMGDKLCKKAEELVKPGMLGPFCLQSLCTENLELVVFEMSARSDGGNN
TFMNGSPYSYLYNAEPLSMGQRIAKEIKLALELKMIEKVIS

Specific function: Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'- monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1- beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates

COG id: COG1759

COG function: function code R; ATP-utilizing enzymes of ATP-grasp superfamily (probably carboligases)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURP_METVS (P14027)

Other databases:

- EMBL:   X15972
- EMBL:   X15972
- EMBL:   CP000742
- PIR:   S06626
- PIR:   S06627
- RefSeq:   YP_001323201.1
- ProteinModelPortal:   P14027
- SMR:   P14027
- STRING:   P14027
- GeneID:   5324980
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_0683
- eggNOG:   arNOG04185
- HOGENOM:   HBG392202
- OMA:   VFEISAR
- ProtClustDB:   PRK13278
- HAMAP:   MF_01163
- InterPro:   IPR011761
- InterPro:   IPR013816
- InterPro:   IPR009720
- InterPro:   IPR010672
- InterPro:   IPR013817
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20

Pfam domain/function: PF06849 DUF1246; PF06973 DUF1297

EC number: NA

Molecular weight: Translated: 40683; Mature: 40683

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 27-27 BINDING 94-94 BINDING 230-230 BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPKEEIMGIFEKYNKDEITIATVGSHTSLHILKGAKLEGFSTAVITTKDRDVPYKRFGV
CCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCCHHHHCC
ADKFIYVDKFSDISSEEIQEQLREMNAIVVPHGSFIAYCGLDNVESTFKVPMFGNRAILR
CCEEEEEECCCCCCHHHHHHHHHHCCEEEEECCCEEEEECCCCCCCEEECCCCCCCEEEE
WEAERDLEGQLLGGSGLRLPKKYNSPDEIDGPVMVKFPGARGGRGYFPCSSTEEFWRKIN
EECCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHH
DFKAKGVLSEEDVKKAHIEEYVVGANYCIHYFYSPLKDRVELMGIDRRYESSIDGLVRVP
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHCCCCCEEECC
SKDQLELDIDPSYVITGNFPVVIRESLLPQVFDMGDKLCKKAEELVKPGMLGPFCLQSLC
CCCCEEEECCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TENLELVVFEMSARSDGGNNTFMNGSPYSYLYNAEPLSMGQRIAKEIKLALELKMIEKVI
CCCCEEEEEEEECCCCCCCCCEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
S
C
>Mature Secondary Structure
MIPKEEIMGIFEKYNKDEITIATVGSHTSLHILKGAKLEGFSTAVITTKDRDVPYKRFGV
CCCHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCCHHHHCC
ADKFIYVDKFSDISSEEIQEQLREMNAIVVPHGSFIAYCGLDNVESTFKVPMFGNRAILR
CCEEEEEECCCCCCHHHHHHHHHHCCEEEEECCCEEEEECCCCCCCEEECCCCCCCEEEE
WEAERDLEGQLLGGSGLRLPKKYNSPDEIDGPVMVKFPGARGGRGYFPCSSTEEFWRKIN
EECCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHH
DFKAKGVLSEEDVKKAHIEEYVVGANYCIHYFYSPLKDRVELMGIDRRYESSIDGLVRVP
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHCCCCCEEECC
SKDQLELDIDPSYVITGNFPVVIRESLLPQVFDMGDKLCKKAEELVKPGMLGPFCLQSLC
CCCCEEEECCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TENLELVVFEMSARSDGGNNTFMNGSPYSYLYNAEPLSMGQRIAKEIKLALELKMIEKVI
CCCCEEEEEEEECCCCCCCCCEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2475640