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The map label for this gene is lpdA [C]

Identifier: 150399394

GI number: 150399394

Start: 692509

End: 693663

Strand: Reverse

Name: lpdA [C]

Synonym: Mevan_0643

Alternate gene names: 150399394

Gene position: 693663-692509 (Counterclockwise)

Preceding gene: 150399397

Following gene: 150399393

Centisome position: 40.33

GC content: 31.95

Gene sequence:

>1155_bases
ATGATTGCAGTTGTCGGCGCAGGGCCTGCGGGATTATCATGTGCAAGCATGCTTTCCAAATTTGGACTTTCAGTTGAACT
CTTTGAACGAGATAAGTTAGGAGGCACTTGTCTAAATTATGGTTGTAGGTATATTAATGCATTAAAAGATGTTTCAGACA
CTATTGAAAGTTTAAATTCAATAAAAGGTAAAAAACACACTTTAAATGATATACTCTCTTTAAGCGATTTACATAACAAG
ATAGATAATATTCATGAGGTAATGAGGGAAAATTCTCTTGAATCCCTTACTAAAAAAGGAATAGTAGTTAAATTTAAAGA
ATTTAAAGAAGAATATGAAAAAGATTATGATTACGTGGTTTATGCAACAGGTTATGACTATCCTACCAGTTTTGAAGGCG
TAGAATGTGCAAAATACAATGAAATTCCATATATAAGAAAATTACCTAAAAAAGTCCTTGTAATTGGGGGGGGCACCGTT
GCTGCAGAATATGCATCCATATTTTCAACTTTTGGAAGTGATGTAACAGTATATGTTCGATCAAAATTTTTAAAAATGAT
TGAAGATGATGAAGTTCGAGAGTATATCATAAACAACATTTCTAATTTTAAAATAACGAGTGATATCAACGTAATGAGAA
AAATGTTAAATGATAATGAGTATTTTAATGTATTGGCTGTTGGAGGTACACCTAAATATAAAACAAACGAATTTCTTCAA
GTAGATGGAAAAAATAATGTTTATGCCTGTGGTGATGCTGTGAAAGGGGGCTACACTCCGATTGCAAATCGAGAAGGAAA
ACTTGTTGCAGAAAATATCTATAACGCCATAAAAGGTATTCCTTTAAAAAAAATGACCTATGGTATAAATATTTCTACAA
TTAGGATGCCTATGAATATTTCAGTACTTGGAAAACAGACATTAAACTTTAAAACAACATATAATCGCCCAGGTAGTGGT
TACTATTTTAAAAAGTCCGAAAAAAGAGGAATGAATAGGATTTATTATGAAAATGGAAAAGTTGTTGGTGCAATTGCAAT
GACTCCCGCAACGGATGTTTCCCCTTATTTTTTACAATATATTAAGGGAATTGATGTTTACAATGAATTTTTAGAGGTTT
ATCCTTCAAACGACCCGTTCTACTGGCAGGTATAA

Upstream 100 bases:

>100_bases
CTCCAAAAGTGTTCAACATATCACCAGAATATTTTCGTAAGATTTAAACATGTTACTTGAAATATTATACAATACTACTT
TAAAAAATGAGGGGATTTTT

Downstream 100 bases:

>100_bases
ATAGGAATATGCGATAAGTATTATGAAAAAAGGGTAAAAAATGGATTTAACCAAATTAGGAATAGGCCTTGTTGAAGAGT
ATGGTTATTATGCCCTATTT

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: protein N6-(lipoyl)lysine; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MIAVVGAGPAGLSCASMLSKFGLSVELFERDKLGGTCLNYGCRYINALKDVSDTIESLNSIKGKKHTLNDILSLSDLHNK
IDNIHEVMRENSLESLTKKGIVVKFKEFKEEYEKDYDYVVYATGYDYPTSFEGVECAKYNEIPYIRKLPKKVLVIGGGTV
AAEYASIFSTFGSDVTVYVRSKFLKMIEDDEVREYIINNISNFKITSDINVMRKMLNDNEYFNVLAVGGTPKYKTNEFLQ
VDGKNNVYACGDAVKGGYTPIANREGKLVAENIYNAIKGIPLKKMTYGINISTIRMPMNISVLGKQTLNFKTTYNRPGSG
YYFKKSEKRGMNRIYYENGKVVGAIAMTPATDVSPYFLQYIKGIDVYNEFLEVYPSNDPFYWQV

Sequences:

>Translated_384_residues
MIAVVGAGPAGLSCASMLSKFGLSVELFERDKLGGTCLNYGCRYINALKDVSDTIESLNSIKGKKHTLNDILSLSDLHNK
IDNIHEVMRENSLESLTKKGIVVKFKEFKEEYEKDYDYVVYATGYDYPTSFEGVECAKYNEIPYIRKLPKKVLVIGGGTV
AAEYASIFSTFGSDVTVYVRSKFLKMIEDDEVREYIINNISNFKITSDINVMRKMLNDNEYFNVLAVGGTPKYKTNEFLQ
VDGKNNVYACGDAVKGGYTPIANREGKLVAENIYNAIKGIPLKKMTYGINISTIRMPMNISVLGKQTLNFKTTYNRPGSG
YYFKKSEKRGMNRIYYENGKVVGAIAMTPATDVSPYFLQYIKGIDVYNEFLEVYPSNDPFYWQV
>Mature_384_residues
MIAVVGAGPAGLSCASMLSKFGLSVELFERDKLGGTCLNYGCRYINALKDVSDTIESLNSIKGKKHTLNDILSLSDLHNK
IDNIHEVMRENSLESLTKKGIVVKFKEFKEEYEKDYDYVVYATGYDYPTSFEGVECAKYNEIPYIRKLPKKVLVIGGGTV
AAEYASIFSTFGSDVTVYVRSKFLKMIEDDEVREYIINNISNFKITSDINVMRKMLNDNEYFNVLAVGGTPKYKTNEFLQ
VDGKNNVYACGDAVKGGYTPIANREGKLVAENIYNAIKGIPLKKMTYGINISTIRMPMNISVLGKQTLNFKTTYNRPGSG
YYFKKSEKRGMNRIYYENGKVVGAIAMTPATDVSPYFLQYIKGIDVYNEFLEVYPSNDPFYWQV

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.4

Molecular weight: Translated: 43290; Mature: 43290

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAVVGAGPAGLSCASMLSKFGLSVELFERDKLGGTCLNYGCRYINALKDVSDTIESLNS
CEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IKGKKHTLNDILSLSDLHNKIDNIHEVMRENSLESLTKKGIVVKFKEFKEEYEKDYDYVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCEEEEHHHHHHHHCCCCCEEE
YATGYDYPTSFEGVECAKYNEIPYIRKLPKKVLVIGGGTVAAEYASIFSTFGSDVTVYVR
EEECCCCCCCCCCCEECCCCCCCHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEHH
SKFLKMIEDDEVREYIINNISNFKITSDINVMRKMLNDNEYFNVLAVGGTPKYKTNEFLQ
HHHHHHHCCHHHHHHHHCCCCCEEEECHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEE
VDGKNNVYACGDAVKGGYTPIANREGKLVAENIYNAIKGIPLKKMTYGINISTIRMPMNI
ECCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHEEECCEEEEEEECCEE
SVLGKQTLNFKTTYNRPGSGYYFKKSEKRGMNRIYYENGKVVGAIAMTPATDVSPYFLQY
EEECCCEEEEEEECCCCCCCEEEECHHHCCCCEEEEECCEEEEEEEECCCCCCCHHHHHH
IKGIDVYNEFLEVYPSNDPFYWQV
HHHHHHHHHHHHHCCCCCCEEEEC
>Mature Secondary Structure
MIAVVGAGPAGLSCASMLSKFGLSVELFERDKLGGTCLNYGCRYINALKDVSDTIESLNS
CEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IKGKKHTLNDILSLSDLHNKIDNIHEVMRENSLESLTKKGIVVKFKEFKEEYEKDYDYVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCEEEEHHHHHHHHCCCCCEEE
YATGYDYPTSFEGVECAKYNEIPYIRKLPKKVLVIGGGTVAAEYASIFSTFGSDVTVYVR
EEECCCCCCCCCCCEECCCCCCCHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEHH
SKFLKMIEDDEVREYIINNISNFKITSDINVMRKMLNDNEYFNVLAVGGTPKYKTNEFLQ
HHHHHHHCCHHHHHHHHCCCCCEEEECHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEE
VDGKNNVYACGDAVKGGYTPIANREGKLVAENIYNAIKGIPLKKMTYGINISTIRMPMNI
ECCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHEEECCEEEEEEECCEE
SVLGKQTLNFKTTYNRPGSGYYFKKSEKRGMNRIYYENGKVVGAIAMTPATDVSPYFLQY
EEECCCEEEEEEECCCCCCCEEEECHHHCCCCEEEEECCEEEEEEEECCCCCCCHHHHHH
IKGIDVYNEFLEVYPSNDPFYWQV
HHHHHHHHHHHHHCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADH [C]

Metal ions: NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {lipoamide}} 1.1 {NAD+}} [C]

Substrates: protein N6-(dihydrolipoyl)lysine; NAD+

Specific reaction: protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; p-Aminophenyl dichloroarsine [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]