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The map label for this gene is fen [H]

Identifier: 150399374

GI number: 150399374

Start: 677511

End: 678485

Strand: Reverse

Name: fen [H]

Synonym: Mevan_0623

Alternate gene names: 150399374

Gene position: 678485-677511 (Counterclockwise)

Preceding gene: 150399375

Following gene: 150399373

Centisome position: 39.45

GC content: 30.97

Gene sequence:

>975_bases
ATGGGAGTTCAGTTTGGAGATATAATTCCTAAAAAAGAAATCTCTTTAAAACTTTTAAGAACTAAATCAGCAGCAATAGA
TGCAATGAATGTAATTTACCAGTTTTTATCAAGCATTCGCCTAAAAGACGGGAGTCCTTTAAAAAATAGGAGTGGGGAGA
TTACATCAACTTATAATGGTATATTTTATAAAACAATATATATGTTGGAAAACGAAATAACTCCAATATGGGTTTTTGAT
GGAAAATCGCATGATTTAAAAGAAAAAACCAAAGAAGATAGGCGAAAATTGCGCCAAAACGCACTTGAAAATTACCTCGA
AGCAAAAGAACAGGATAATTTAGAAAATATGCAAAAATATGCAAAAAGAGCTAATTTTCTAGATAAAAAAACAATTGAAA
ATTCTAAACGGCTTTTAGAACTCATGGGTGTCCCATATATTAATGCACCTTCCGAAGGAGAAGCCCAATGTGCAGAACTT
GTAAAATCAAAAAATGCGTCATTTGTAGTAAGTCAAGATTATGATTCTATTCTTTATGGTGCAGAAAGCGTTGTAAAAAA
CATAACTTCGTCAAACAAGAGTCTAGAATTGATTGAACTTTCAAAAGTTTTAACTGAACTAAATGTTAATCTTTTAGAAC
TAATTGATGTAGCGATATTAATTGGTACAGATTATAATCCAGGGGGAATTAAGGGAATTGGCCCAAAAAAAGCATTTGAA
GTTGTCAAAAAAGGGCAAATGGAAAAATATGCTTTTGAAATTCAAAACTATTCTGAAATTAGAAAAATATTTGATGAACC
AAACGTAATAACTGACTACGAAATCGGGTTAAAACTTCCAAGAAAAGACGAACTAATTGAATTTTTAGTGGAAGAAAATG
ACTTTTCAAAAGAACGGGTATTACCCAATATCGAAAAACTAGATTTACTGTTGGGTAACAAAAAATCCCAAAAAAGCCTT
GAATCGTGGTTTTAG

Upstream 100 bases:

>100_bases
GGGCTTAAGAGAAAAAGTACTTAATAGATTTTTCGAAATAGTTAATGGAATGAATCAAAATGTCTAACTTATTTTATATT
ACCTCTTAATTGGTGTTTTT

Downstream 100 bases:

>100_bases
GTGATTTTAAAAATGATTAAAGTATACAGTTTAAATAAAATAACTCCAATATTTTGGATTTTTCTAATTCCCATCGTATT
AATTGGGTTATTTTTAATTA

Product: flap endonuclease-1

Products: NA

Alternate protein names: FEN-1; Flap structure-specific endonuclease 1 [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MGVQFGDIIPKKEISLKLLRTKSAAIDAMNVIYQFLSSIRLKDGSPLKNRSGEITSTYNGIFYKTIYMLENEITPIWVFD
GKSHDLKEKTKEDRRKLRQNALENYLEAKEQDNLENMQKYAKRANFLDKKTIENSKRLLELMGVPYINAPSEGEAQCAEL
VKSKNASFVVSQDYDSILYGAESVVKNITSSNKSLELIELSKVLTELNVNLLELIDVAILIGTDYNPGGIKGIGPKKAFE
VVKKGQMEKYAFEIQNYSEIRKIFDEPNVITDYEIGLKLPRKDELIEFLVEENDFSKERVLPNIEKLDLLLGNKKSQKSL
ESWF

Sequences:

>Translated_324_residues
MGVQFGDIIPKKEISLKLLRTKSAAIDAMNVIYQFLSSIRLKDGSPLKNRSGEITSTYNGIFYKTIYMLENEITPIWVFD
GKSHDLKEKTKEDRRKLRQNALENYLEAKEQDNLENMQKYAKRANFLDKKTIENSKRLLELMGVPYINAPSEGEAQCAEL
VKSKNASFVVSQDYDSILYGAESVVKNITSSNKSLELIELSKVLTELNVNLLELIDVAILIGTDYNPGGIKGIGPKKAFE
VVKKGQMEKYAFEIQNYSEIRKIFDEPNVITDYEIGLKLPRKDELIEFLVEENDFSKERVLPNIEKLDLLLGNKKSQKSL
ESWF
>Mature_323_residues
GVQFGDIIPKKEISLKLLRTKSAAIDAMNVIYQFLSSIRLKDGSPLKNRSGEITSTYNGIFYKTIYMLENEITPIWVFDG
KSHDLKEKTKEDRRKLRQNALENYLEAKEQDNLENMQKYAKRANFLDKKTIENSKRLLELMGVPYINAPSEGEAQCAELV
KSKNASFVVSQDYDSILYGAESVVKNITSSNKSLELIELSKVLTELNVNLLELIDVAILIGTDYNPGGIKGIGPKKAFEV
VKKGQMEKYAFEIQNYSEIRKIFDEPNVITDYEIGLKLPRKDELIEFLVEENDFSKERVLPNIEKLDLLLGNKKSQKSLE
SWF

Specific function: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymeras

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758356, Length=330, Percent_Identity=35.7575757575758, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI194018535, Length=241, Percent_Identity=24.4813278008299, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI194018531, Length=241, Percent_Identity=24.4813278008299, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI51988900, Length=142, Percent_Identity=34.5070422535211, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17510005, Length=327, Percent_Identity=34.5565749235474, Blast_Score=177, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6322736, Length=307, Percent_Identity=36.1563517915309, Blast_Score=176, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6321697, Length=111, Percent_Identity=35.1351351351351, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320469, Length=243, Percent_Identity=27.5720164609054, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17647423, Length=337, Percent_Identity=35.0148367952522, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI78706850, Length=109, Percent_Identity=37.6146788990826, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI78706852, Length=109, Percent_Identity=37.6146788990826, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI78706854, Length=109, Percent_Identity=37.6146788990826, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24658219, Length=245, Percent_Identity=25.3061224489796, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI17137168, Length=241, Percent_Identity=26.1410788381743, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020045
- InterPro:   IPR019973
- InterPro:   IPR008918
- InterPro:   IPR006086
- InterPro:   IPR006085
- InterPro:   IPR006084 [H]

Pfam domain/function: PF00867 XPG_I; PF00752 XPG_N [H]

EC number: 3.-.-.-

Molecular weight: Translated: 37025; Mature: 36894

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVQFGDIIPKKEISLKLLRTKSAAIDAMNVIYQFLSSIRLKDGSPLKNRSGEITSTYNG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCC
IFYKTIYMLENEITPIWVFDGKSHDLKEKTKEDRRKLRQNALENYLEAKEQDNLENMQKY
HHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AKRANFLDKKTIENSKRLLELMGVPYINAPSEGEAQCAELVKSKNASFVVSQDYDSILYG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHH
AESVVKNITSSNKSLELIELSKVLTELNVNLLELIDVAILIGTDYNPGGIKGIGPKKAFE
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCCCCCCCHHHHHH
VVKKGQMEKYAFEIQNYSEIRKIFDEPNVITDYEIGLKLPRKDELIEFLVEENDFSKERV
HHHHCCHHHHHHHHCCHHHHHHHHCCCCEEECHHCCCCCCCHHHHHHHHHCCCCCCHHHC
LPNIEKLDLLLGNKKSQKSLESWF
CCCHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
GVQFGDIIPKKEISLKLLRTKSAAIDAMNVIYQFLSSIRLKDGSPLKNRSGEITSTYNG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCC
IFYKTIYMLENEITPIWVFDGKSHDLKEKTKEDRRKLRQNALENYLEAKEQDNLENMQKY
HHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AKRANFLDKKTIENSKRLLELMGVPYINAPSEGEAQCAELVKSKNASFVVSQDYDSILYG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCHHHHHHH
AESVVKNITSSNKSLELIELSKVLTELNVNLLELIDVAILIGTDYNPGGIKGIGPKKAFE
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCCCCCCCHHHHHH
VVKKGQMEKYAFEIQNYSEIRKIFDEPNVITDYEIGLKLPRKDELIEFLVEENDFSKERV
HHHHCCHHHHHHHHCCHHHHHHHHCCCCEEECHHCCCCCCCHHHHHHHHHCCCCCCHHHC
LPNIEKLDLLLGNKKSQKSLESWF
CCCHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA