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The map label for this gene is rfbB [C]

Identifier: 150399165

GI number: 150399165

Start: 465221

End: 466180

Strand: Reverse

Name: rfbB [C]

Synonym: Mevan_0412

Alternate gene names: 150399165

Gene position: 466180-465221 (Counterclockwise)

Preceding gene: 150399166

Following gene: 150399164

Centisome position: 27.1

GC content: 26.67

Gene sequence:

>960_bases
ATGAAAATTTTAGTTACTGGTGCAGCTGGGTTTATAGGGTTTAGTTTTTCTGAAAATATTTTAAAAAATACTGAAATTCA
AATAATTGGGATTGATAATTTAAATCCATACTATGATGTAAAATTAAAAGAAAAAAGGCTTGACATTTTAAAAGGATTTA
AAAATTTTAAGTTTTTTGATGAAGATATTATAAATTACGAAGAATTAGATGAAATTTTCAATTTAGAAAAACCTGATTTA
ATAGTCCATCTTGCAGCACAGGCTGGAGTTAGGTACTCTTTAGAAAATCCGCATGTTTATGAATACAGTAATAATTTAGG
AACATTAAATATTTTTGAACTTGCTAAAAAATACGGTATTAAAAGAGTTATTTTTGCATCATCTTCATCAGTATATGGCG
GAAACGAAAAAACACCTTTTTTGGAATCAGATAATGTCGATAAACCGGTTAGTTTATATGCTGCAACTAAAAAATATAAT
GAATTAATTGCACATGTTTATAATCATTTATATGGGATAGAAATGATTGGTTTAAGATTTTTCACGGTTTATGGTGAATT
TGGAAGGCCCGATATGGCTTACTGGAAATTCACTAAAAATATATTGGAAGAAAAACCAATTGATGTTTATAATTACGGAA
AAATGAAAAGGGATTTTACATATATTTCAGATATAGTTGAAGGGATAAAATCAGCAGTTTTTTTAAGTAAAAAAATAGAT
TATGAAATATTTAATCTTGGCGGGGATAACCCTATTGAATTAGAATACATGATTTCCTTAATAGAAAAAGAAACTGGGAA
AAAAGCCATTAAAAATTACTTACCCGTGCAAGAAGGGGATGTTTTAATTACAATGGCAGACTTAACAAAATCAAAAGAAA
ATTTAAAATATTATCCTAATATATGCATAGAAGAAGGAATAAAAAGGTTTGTTAAATGGTATATTCAAAATAGGTATTAA

Upstream 100 bases:

>100_bases
TTAATATGGTTAATGAAAAAGTCATATTTGATGGCAGAAATATAATTGATAATGAAAAAATTAAAAAAATCGAGTTTGAG
TATATTGGAGTTGGATTAAG

Downstream 100 bases:

>100_bases
ACTATAAACTGGGAAAAAATGAAATTACTTTATTTAGCCGATGGATCAAGTACTCATACATATAAATACATTAAATACTT
TTTAGAAAAAGATTGTTACG

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKILVTGAAGFIGFSFSENILKNTEIQIIGIDNLNPYYDVKLKEKRLDILKGFKNFKFFDEDIINYEELDEIFNLEKPDL
IVHLAAQAGVRYSLENPHVYEYSNNLGTLNIFELAKKYGIKRVIFASSSSVYGGNEKTPFLESDNVDKPVSLYAATKKYN
ELIAHVYNHLYGIEMIGLRFFTVYGEFGRPDMAYWKFTKNILEEKPIDVYNYGKMKRDFTYISDIVEGIKSAVFLSKKID
YEIFNLGGDNPIELEYMISLIEKETGKKAIKNYLPVQEGDVLITMADLTKSKENLKYYPNICIEEGIKRFVKWYIQNRY

Sequences:

>Translated_319_residues
MKILVTGAAGFIGFSFSENILKNTEIQIIGIDNLNPYYDVKLKEKRLDILKGFKNFKFFDEDIINYEELDEIFNLEKPDL
IVHLAAQAGVRYSLENPHVYEYSNNLGTLNIFELAKKYGIKRVIFASSSSVYGGNEKTPFLESDNVDKPVSLYAATKKYN
ELIAHVYNHLYGIEMIGLRFFTVYGEFGRPDMAYWKFTKNILEEKPIDVYNYGKMKRDFTYISDIVEGIKSAVFLSKKID
YEIFNLGGDNPIELEYMISLIEKETGKKAIKNYLPVQEGDVLITMADLTKSKENLKYYPNICIEEGIKRFVKWYIQNRY
>Mature_319_residues
MKILVTGAAGFIGFSFSENILKNTEIQIIGIDNLNPYYDVKLKEKRLDILKGFKNFKFFDEDIINYEELDEIFNLEKPDL
IVHLAAQAGVRYSLENPHVYEYSNNLGTLNIFELAKKYGIKRVIFASSSSVYGGNEKTPFLESDNVDKPVSLYAATKKYN
ELIAHVYNHLYGIEMIGLRFFTVYGEFGRPDMAYWKFTKNILEEKPIDVYNYGKMKRDFTYISDIVEGIKSAVFLSKKID
YEIFNLGGDNPIELEYMISLIEKETGKKAIKNYLPVQEGDVLITMADLTKSKENLKYYPNICIEEGIKRFVKWYIQNRY

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=323, Percent_Identity=27.2445820433437, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI42516563, Length=326, Percent_Identity=27.6073619631902, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI56237023, Length=340, Percent_Identity=25, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI56118217, Length=340, Percent_Identity=25, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI189083684, Length=340, Percent_Identity=25, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788353, Length=343, Percent_Identity=25.6559766763848, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI48994969, Length=338, Percent_Identity=26.3313609467456, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1786974, Length=341, Percent_Identity=25.8064516129032, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788589, Length=352, Percent_Identity=23.8636363636364, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539532, Length=327, Percent_Identity=26.2996941896024, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71982035, Length=347, Percent_Identity=27.0893371757925, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71982038, Length=349, Percent_Identity=27.2206303724928, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17568069, Length=275, Percent_Identity=29.0909090909091, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=361, Percent_Identity=24.0997229916898, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356223, Length=325, Percent_Identity=27.0769230769231, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19923002, Length=342, Percent_Identity=26.3157894736842, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46

Molecular weight: Translated: 37014; Mature: 37014

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGAAGFIGFSFSENILKNTEIQIIGIDNLNPYYDVKLKEKRLDILKGFKNFKFFD
CEEEEEECCCCEEECCCHHHHCCCCEEEEEECCCCCEEEEEEHHHHHHHHHHHHCCCCHH
EDIINYEELDEIFNLEKPDLIVHLAAQAGVRYSLENPHVYEYSNNLGTLNIFELAKKYGI
HHHCCHHHHHHHHCCCCCCEEEEEECCCCCEEECCCCEEEEECCCCCCCHHHHHHHHHCC
KRVIFASSSSVYGGNEKTPFLESDNVDKPVSLYAATKKYNELIAHVYNHLYGIEMIGLRF
EEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
FTVYGEFGRPDMAYWKFTKNILEEKPIDVYNYGKMKRDFTYISDIVEGIKSAVFLSKKID
EEEECCCCCCCHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
YEIFNLGGDNPIELEYMISLIEKETGKKAIKNYLPVQEGDVLITMADLTKSKENLKYYPN
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHCCCCCCEECCC
ICIEEGIKRFVKWYIQNRY
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKILVTGAAGFIGFSFSENILKNTEIQIIGIDNLNPYYDVKLKEKRLDILKGFKNFKFFD
CEEEEEECCCCEEECCCHHHHCCCCEEEEEECCCCCEEEEEEHHHHHHHHHHHHCCCCHH
EDIINYEELDEIFNLEKPDLIVHLAAQAGVRYSLENPHVYEYSNNLGTLNIFELAKKYGI
HHHCCHHHHHHHHCCCCCCEEEEEECCCCCEEECCCCEEEEECCCCCCCHHHHHHHHHCC
KRVIFASSSSVYGGNEKTPFLESDNVDKPVSLYAATKKYNELIAHVYNHLYGIEMIGLRF
EEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
FTVYGEFGRPDMAYWKFTKNILEEKPIDVYNYGKMKRDFTYISDIVEGIKSAVFLSKKID
EEEECCCCCCCHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
YEIFNLGGDNPIELEYMISLIEKETGKKAIKNYLPVQEGDVLITMADLTKSKENLKYYPN
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHCCCCCCEECCC
ICIEEGIKRFVKWYIQNRY
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose

Specific reaction: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]