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The map label for this gene is exoA [H]

Identifier: 150399093

GI number: 150399093

Start: 388141

End: 388914

Strand: Reverse

Name: exoA [H]

Synonym: Mevan_0339

Alternate gene names: 150399093

Gene position: 388914-388141 (Counterclockwise)

Preceding gene: 150399098

Following gene: 150399092

Centisome position: 22.61

GC content: 30.62

Gene sequence:

>774_bases
ATGATTTTTGAAATAACGTATATTATGAAAATGATATCATGGAATGTAAATGGAATTAGGGCCTGTTTAAAAAACGGCTT
CATGGATTTTTTAAATTCGAAAAATCCTGAAATAATATGTATTCAAGAAACCAAAGTCCAAAAAGGTCAGGTACAGCTTG
GATTAAACGGTTACTTTGAATACTGGAATTACGCTGAAAAGAAAGGCTATTCTGGAACCGCTATTTTTACTAAAATAGAG
CCAAAAAGTGTGAAATACGGTATAACTAATTACAAAAGTGATAAAGAAGGCCGTGTAATTACTCTTGAATTTGAAGAATA
TTTTTTGGTAAATGTATACACTCCAAATTCACAAAGGGGTCTTAAAAGATTAGAATGTCGGCAAAAATGGGACAAAGATT
TTTTAAATCATTTAAAAACTCTTGAACAAAAAAAGCCTGTGATATTTTGTGGGGATTTAAATGTTGCCCATAAAGAAATA
GATTTGAAAAACCCAAAGCAAAATATAAAAAACGCAGGTTTTACAAAGGAAGAACGAAATGGCTTTGACAATTTAATAAA
TTCAGGTTTTTTAGATACATTTAGGGAATTAAATAACGAACCTGAAAACTACACTTGGTGGAGCTACCGTTTTAATGCAA
GGGAAAAAAACATAGGTTGGAGAATAGATTATTTTTGCATTTCAAAAAAATTACGAGAACGATTAAAAGATGCATTTATA
ATGTCAGAAGTACTTGGTTCAGACCACTGCCCTGTTGGAATTATCATTGAATAA

Upstream 100 bases:

>100_bases
AAATTTACTTAAATTAAACCAAATTTAATAGTGAAGTTAACTTTAGCAATAAAATTACATTTTATATAATCTCTTTTTTA
CGATATTAAAATGTATTTAT

Downstream 100 bases:

>100_bases
TATCGTACGAATAAACCTAAAACTTTTTATTTTTAGATAATGGCTAAATCGGTAAGATTTATAAACTAAACCACATTACA
TTTAAGAGATTACTTTTCAA

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MIFEITYIMKMISWNVNGIRACLKNGFMDFLNSKNPEIICIQETKVQKGQVQLGLNGYFEYWNYAEKKGYSGTAIFTKIE
PKSVKYGITNYKSDKEGRVITLEFEEYFLVNVYTPNSQRGLKRLECRQKWDKDFLNHLKTLEQKKPVIFCGDLNVAHKEI
DLKNPKQNIKNAGFTKEERNGFDNLINSGFLDTFRELNNEPENYTWWSYRFNAREKNIGWRIDYFCISKKLRERLKDAFI
MSEVLGSDHCPVGIIIE

Sequences:

>Translated_257_residues
MIFEITYIMKMISWNVNGIRACLKNGFMDFLNSKNPEIICIQETKVQKGQVQLGLNGYFEYWNYAEKKGYSGTAIFTKIE
PKSVKYGITNYKSDKEGRVITLEFEEYFLVNVYTPNSQRGLKRLECRQKWDKDFLNHLKTLEQKKPVIFCGDLNVAHKEI
DLKNPKQNIKNAGFTKEERNGFDNLINSGFLDTFRELNNEPENYTWWSYRFNAREKNIGWRIDYFCISKKLRERLKDAFI
MSEVLGSDHCPVGIIIE
>Mature_257_residues
MIFEITYIMKMISWNVNGIRACLKNGFMDFLNSKNPEIICIQETKVQKGQVQLGLNGYFEYWNYAEKKGYSGTAIFTKIE
PKSVKYGITNYKSDKEGRVITLEFEEYFLVNVYTPNSQRGLKRLECRQKWDKDFLNHLKTLEQKKPVIFCGDLNVAHKEI
DLKNPKQNIKNAGFTKEERNGFDNLINSGFLDTFRELNNEPENYTWWSYRFNAREKNIGWRIDYFCISKKLRERLKDAFI
MSEVLGSDHCPVGIIIE

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=255, Percent_Identity=47.0588235294118, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI18375503, Length=255, Percent_Identity=47.0588235294118, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI18375501, Length=255, Percent_Identity=47.0588235294118, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI18375507, Length=315, Percent_Identity=26.3492063492064, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=28.7313432835821, Blast_Score=104, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71989536, Length=253, Percent_Identity=39.1304347826087, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI71989539, Length=144, Percent_Identity=31.25, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI221330655, Length=250, Percent_Identity=46.8, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI17136678, Length=250, Percent_Identity=46.8, Blast_Score=237, Evalue=5e-63,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30286; Mature: 30286

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFEITYIMKMISWNVNGIRACLKNGFMDFLNSKNPEIICIQETKVQKGQVQLGLNGYFE
CEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCHHHH
YWNYAEKKGYSGTAIFTKIEPKSVKYGITNYKSDKEGRVITLEFEEYFLVNVYTPNSQRG
HHHHHHHCCCCCCEEEEEECCCCEEECCCCCCCCCCCCEEEEEECCEEEEEEECCCCHHH
LKRLECRQKWDKDFLNHLKTLEQKKPVIFCGDLNVAHKEIDLKNPKQNIKNAGFTKEERN
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHCCCCCCCHHHHHCCCCCCHHHC
GFDNLINSGFLDTFRELNNEPENYTWWSYRFNAREKNIGWRIDYFCISKKLRERLKDAFI
CHHHHHHCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHH
MSEVLGSDHCPVGIIIE
HHHHHCCCCCCEEEEEC
>Mature Secondary Structure
MIFEITYIMKMISWNVNGIRACLKNGFMDFLNSKNPEIICIQETKVQKGQVQLGLNGYFE
CEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCHHHH
YWNYAEKKGYSGTAIFTKIEPKSVKYGITNYKSDKEGRVITLEFEEYFLVNVYTPNSQRG
HHHHHHHCCCCCCEEEEEECCCCEEECCCCCCCCCCCCEEEEEECCEEEEEEECCCCHHH
LKRLECRQKWDKDFLNHLKTLEQKKPVIFCGDLNVAHKEIDLKNPKQNIKNAGFTKEERN
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHCCCCCCCHHHHHCCCCCCHHHC
GFDNLINSGFLDTFRELNNEPENYTWWSYRFNAREKNIGWRIDYFCISKKLRERLKDAFI
CHHHHHHCCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHH
MSEVLGSDHCPVGIIIE
HHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]