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The map label for this gene is gltX

Identifier: 150399092

GI number: 150399092

Start: 386336

End: 388003

Strand: Reverse

Name: gltX

Synonym: Mevan_0338

Alternate gene names: 150399092

Gene position: 388003-386336 (Counterclockwise)

Preceding gene: 150399093

Following gene: 150399091

Centisome position: 22.56

GC content: 33.69

Gene sequence:

>1668_bases
ATGAAAGATACAGTAATGGTTTATTTACTAGAAAACTCAATTAAATTTAACGGAAAACCAAATCCAAAGGCAGTAATGGG
TAAAATTCTTGGTGGGAATCCCGATTTAAGAAACAGGGTTAATGAACTAAATGAAGTTATTTCAAAGGTTTTGAAAGAAA
TTGAAGTAATGTCTCTTGAAGAACAGCAAATTAAATTAAATGAAATTGCTCCGGAAGGTTTAGTGCAAAAAACTGAACGA
AAAAGAAAAGAAATTGAATTAAAAAATGCAAAAGATAAAGTTGTAATGAGATTTGCACCAAACCCCTCTGGACCATTACA
TATCGGGCATGCAAGAGCGTCTGTATTAAACGATTTTTTTGCTAAAAAATACAATGGAAAGTTAATTTTAAGGCTTGAAG
ACACTGATGCAAAACGAGTTCTCCCAGAAGCTTATGAAATGATTGAAGAAGACCTAAAATGGCTTGGAATTAAGGTTGAC
GAAGTAATTATCCAATCAGAAAGACTTGAAATTTACTATGGATACGGCAGAAAATTAATCGAAATGAGTTACGCTTATGT
TTGTGACTGTAATCCTGAAGAATTTAAGGAATTACGGGAAAAAGGAATCCCTTGTAAATGTAGGGAAACAACGCCAGAAA
CAAATCTTAAACTATGGGAAAAAATGCTTGCCGGAGAACTGGATAACGTTGCAGTAAGACTTAAAACGGATATTGGGCAC
AAAAATCCGTCAATTAGGGATTTCCCGATATTTAGAATTGAAAAAACGCCCCACCCTAAAAATGGAACAAAATACCACGT
ATATCCTTTAATGAATCTTTCAGTAACGATTGACGACCACCTGATGGGAATGACCCACGTATTACGGGGAAAAGACCATA
TTATAAATACTGAAAAGCAGGAATATATTTACAAGTATTTTGGATGGGAATCTCCCGAATACATTCACTACGGAATTTTA
AAAATTGAGGGGCCTGTTTTAAGTACGTCAAAAATGCATACAGGAATTTTAAGCGGAGAATATTCCGGATGGGACGATGC
ACGGCTTGGTACACTAAGGGCCCTTAAAAAAAGAGGAATAAAGCCAGAAGCACTATATAAGTTAATGGTTGAAATTGGAA
TAAAGCAAGCAGACGTTAAATTTGCATGGGAAAACTTAAATGCAGCAAATAAGGAAATAATCGATAAAGATGCGAAAAGA
TTTTTCTTTGTCGATAGCCCTAAAAAAGTTATAATTACAGGTTTTAAAAACAAAAAAATTAATTTAAGAATGCACCCTGA
TAGAAGCGACTTTGGAAACCGGGAACTTTTGTTTGATGGCGAAATTTACGTTTCAGACGACCTTGAAGCAGGAAAAATGT
ATAGGTTAATGGAATTATTTAATATCGTTGTAGAAAAGATTGAAAACGGTGTAATTTATGCCCATTATGATAGTGATGAC
ATAAGCGTTGCAAGAACAAACAAAGCAAATATTATTCACTGGATACCGATAAAAGACAGTGTAAAAGTAACCGTAATTGA
TGAAAATGCAGAAAAAATTGAAGGATTTGCAGAAAAAGACTTTTTAATTACGGAAGAGGATGATTTTGTTCAATTTGAGA
GATTTGGATTTGTTAGGATTGACGAAAAATTAGATGATGGATATATCTGCTACTATACTCACAAATAA

Upstream 100 bases:

>100_bases
TAATGGCTAAATCGGTAAGATTTATAAACTAAACCACATTACATTTAAGAGATTACTTTTCAAAAGGATTTAATAAAGGA
CACCAAAAATAGTGATAATT

Downstream 100 bases:

>100_bases
TTTTTTTAGTTATTTTTTACTATCTAATATACTTACGTTTATTTTTGAAATTAAAGGATATTTAAAATTTAAAATTACTT
ATGAGTATTTATAATTAAAT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKDTVMVYLLENSIKFNGKPNPKAVMGKILGGNPDLRNRVNELNEVISKVLKEIEVMSLEEQQIKLNEIAPEGLVQKTER
KRKEIELKNAKDKVVMRFAPNPSGPLHIGHARASVLNDFFAKKYNGKLILRLEDTDAKRVLPEAYEMIEEDLKWLGIKVD
EVIIQSERLEIYYGYGRKLIEMSYAYVCDCNPEEFKELREKGIPCKCRETTPETNLKLWEKMLAGELDNVAVRLKTDIGH
KNPSIRDFPIFRIEKTPHPKNGTKYHVYPLMNLSVTIDDHLMGMTHVLRGKDHIINTEKQEYIYKYFGWESPEYIHYGIL
KIEGPVLSTSKMHTGILSGEYSGWDDARLGTLRALKKRGIKPEALYKLMVEIGIKQADVKFAWENLNAANKEIIDKDAKR
FFFVDSPKKVIITGFKNKKINLRMHPDRSDFGNRELLFDGEIYVSDDLEAGKMYRLMELFNIVVEKIENGVIYAHYDSDD
ISVARTNKANIIHWIPIKDSVKVTVIDENAEKIEGFAEKDFLITEEDDFVQFERFGFVRIDEKLDDGYICYYTHK

Sequences:

>Translated_555_residues
MKDTVMVYLLENSIKFNGKPNPKAVMGKILGGNPDLRNRVNELNEVISKVLKEIEVMSLEEQQIKLNEIAPEGLVQKTER
KRKEIELKNAKDKVVMRFAPNPSGPLHIGHARASVLNDFFAKKYNGKLILRLEDTDAKRVLPEAYEMIEEDLKWLGIKVD
EVIIQSERLEIYYGYGRKLIEMSYAYVCDCNPEEFKELREKGIPCKCRETTPETNLKLWEKMLAGELDNVAVRLKTDIGH
KNPSIRDFPIFRIEKTPHPKNGTKYHVYPLMNLSVTIDDHLMGMTHVLRGKDHIINTEKQEYIYKYFGWESPEYIHYGIL
KIEGPVLSTSKMHTGILSGEYSGWDDARLGTLRALKKRGIKPEALYKLMVEIGIKQADVKFAWENLNAANKEIIDKDAKR
FFFVDSPKKVIITGFKNKKINLRMHPDRSDFGNRELLFDGEIYVSDDLEAGKMYRLMELFNIVVEKIENGVIYAHYDSDD
ISVARTNKANIIHWIPIKDSVKVTVIDENAEKIEGFAEKDFLITEEDDFVQFERFGFVRIDEKLDDGYICYYTHK
>Mature_555_residues
MKDTVMVYLLENSIKFNGKPNPKAVMGKILGGNPDLRNRVNELNEVISKVLKEIEVMSLEEQQIKLNEIAPEGLVQKTER
KRKEIELKNAKDKVVMRFAPNPSGPLHIGHARASVLNDFFAKKYNGKLILRLEDTDAKRVLPEAYEMIEEDLKWLGIKVD
EVIIQSERLEIYYGYGRKLIEMSYAYVCDCNPEEFKELREKGIPCKCRETTPETNLKLWEKMLAGELDNVAVRLKTDIGH
KNPSIRDFPIFRIEKTPHPKNGTKYHVYPLMNLSVTIDDHLMGMTHVLRGKDHIINTEKQEYIYKYFGWESPEYIHYGIL
KIEGPVLSTSKMHTGILSGEYSGWDDARLGTLRALKKRGIKPEALYKLMVEIGIKQADVKFAWENLNAANKEIIDKDAKR
FFFVDSPKKVIITGFKNKKINLRMHPDRSDFGNRELLFDGEIYVSDDLEAGKMYRLMELFNIVVEKIENGVIYAHYDSDD
ISVARTNKANIIHWIPIKDSVKVTVIDENAEKIEGFAEKDFLITEEDDFVQFERFGFVRIDEKLDDGYICYYTHK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI62241042, Length=409, Percent_Identity=31.2958435207824, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI4826960, Length=327, Percent_Identity=29.3577981651376, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI134288884, Length=264, Percent_Identity=32.9545454545455, Blast_Score=141, Evalue=2e-33,
Organism=Escherichia coli, GI1786895, Length=512, Percent_Identity=26.953125, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI1788743, Length=311, Percent_Identity=28.9389067524116, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI226510925, Length=300, Percent_Identity=29, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17511007, Length=470, Percent_Identity=29.1489361702128, Blast_Score=204, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17543332, Length=501, Percent_Identity=26.1477045908184, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71993394, Length=311, Percent_Identity=30.2250803858521, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324742, Length=440, Percent_Identity=28.4090909090909, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321192, Length=526, Percent_Identity=28.1368821292776, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6324540, Length=296, Percent_Identity=34.1216216216216, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24649466, Length=539, Percent_Identity=29.4990723562152, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI17864488, Length=523, Percent_Identity=25.8126195028681, Blast_Score=156, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24665321, Length=330, Percent_Identity=31.8181818181818, Blast_Score=141, Evalue=1e-33,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_METVS (A6UP25)

Other databases:

- EMBL:   CP000742
- RefSeq:   YP_001322859.1
- ProteinModelPortal:   A6UP25
- SMR:   A6UP25
- STRING:   A6UP25
- GeneID:   5325912
- GenomeReviews:   CP000742_GR
- KEGG:   mvn:Mevan_0338
- eggNOG:   arNOG04636
- HOGENOM:   HBG334108
- OMA:   MRFAPNP
- ProtClustDB:   PRK04156
- GO:   GO:0005737
- HAMAP:   MF_00022_A
- InterPro:   IPR001412
- InterPro:   IPR004526
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR022888
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:2.40.240.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00463

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C; SSF50715 Ribosomal_L25rel

EC number: =6.1.1.17

Molecular weight: Translated: 64289; Mature: 64289

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTVMVYLLENSIKFNGKPNPKAVMGKILGGNPDLRNRVNELNEVISKVLKEIEVMSLE
CCCEEEEEEEECCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
EQQIKLNEIAPEGLVQKTERKRKEIELKNAKDKVVMRFAPNPSGPLHIGHARASVLNDFF
HHHEEHHHCCCHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCEEECHHHHHHHHHHH
AKKYNGKLILRLEDTDAKRVLPEAYEMIEEDLKWLGIKVDEVIIQSERLEIYYGYGRKLI
HHHCCCEEEEEECCCCCHHHCHHHHHHHHHHHHHCCCCHHHEEEECCCEEEEECCCCEEE
EMSYAYVCDCNPEEFKELREKGIPCKCRETTPETNLKLWEKMLAGELDNVAVRLKTDIGH
EEEEEEEECCCHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCC
KNPSIRDFPIFRIEKTPHPKNGTKYHVYPLMNLSVTIDDHLMGMTHVLRGKDHIINTEKQ
CCCCCCCCEEEEEECCCCCCCCCEEEEEEEEEEEEEECHHHHHHHHHHCCCCCCCCCCHH
EYIYKYFGWESPEYIHYGILKIEGPVLSTSKMHTGILSGEYSGWDDARLGTLRALKKRGI
HHHHHHHCCCCCCEEEEEEEEECCCEEECCCHHCCEECCCCCCCCCCHHHHHHHHHHCCC
KPEALYKLMVEIGIKQADVKFAWENLNAANKEIIDKDAKRFFFVDSPKKVIITGFKNKKI
CHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHCCCCCEEEEECCCCEEEEEECCCCEE
NLRMHPDRSDFGNRELLFDGEIYVSDDLEAGKMYRLMELFNIVVEKIENGVIYAHYDSDD
EEEECCCCCCCCCEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
ISVARTNKANIIHWIPIKDSVKVTVIDENAEKIEGFAEKDFLITEEDDFVQFERFGFVRI
EEEEECCCCCEEEEEECCCCEEEEEECCCHHHHCCCCCCCEEEECCCCCEEHHHCCEEEE
DEKLDDGYICYYTHK
ECCCCCCEEEEEECC
>Mature Secondary Structure
MKDTVMVYLLENSIKFNGKPNPKAVMGKILGGNPDLRNRVNELNEVISKVLKEIEVMSLE
CCCEEEEEEEECCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
EQQIKLNEIAPEGLVQKTERKRKEIELKNAKDKVVMRFAPNPSGPLHIGHARASVLNDFF
HHHEEHHHCCCHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCCCEEECHHHHHHHHHHH
AKKYNGKLILRLEDTDAKRVLPEAYEMIEEDLKWLGIKVDEVIIQSERLEIYYGYGRKLI
HHHCCCEEEEEECCCCCHHHCHHHHHHHHHHHHHCCCCHHHEEEECCCEEEEECCCCEEE
EMSYAYVCDCNPEEFKELREKGIPCKCRETTPETNLKLWEKMLAGELDNVAVRLKTDIGH
EEEEEEEECCCHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCC
KNPSIRDFPIFRIEKTPHPKNGTKYHVYPLMNLSVTIDDHLMGMTHVLRGKDHIINTEKQ
CCCCCCCCEEEEEECCCCCCCCCEEEEEEEEEEEEEECHHHHHHHHHHCCCCCCCCCCHH
EYIYKYFGWESPEYIHYGILKIEGPVLSTSKMHTGILSGEYSGWDDARLGTLRALKKRGI
HHHHHHHCCCCCCEEEEEEEEECCCEEECCCHHCCEECCCCCCCCCCHHHHHHHHHHCCC
KPEALYKLMVEIGIKQADVKFAWENLNAANKEIIDKDAKRFFFVDSPKKVIITGFKNKKI
CHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHCCCCCEEEEECCCCEEEEEECCCCEE
NLRMHPDRSDFGNRELLFDGEIYVSDDLEAGKMYRLMELFNIVVEKIENGVIYAHYDSDD
EEEECCCCCCCCCEEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
ISVARTNKANIIHWIPIKDSVKVTVIDENAEKIEGFAEKDFLITEEDDFVQFERFGFVRI
EEEEECCCCCEEEEEECCCCEEEEEECCCHHHHCCCCCCCEEEECCCCCEEHHHCCEEEE
DEKLDDGYICYYTHK
ECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA