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The map label for this gene is nifK2 [H]

Identifier: 150398818

GI number: 150398818

Start: 57144

End: 58520

Strand: Reverse

Name: nifK2 [H]

Synonym: Mevan_0059

Alternate gene names: 150398818

Gene position: 58520-57144 (Counterclockwise)

Preceding gene: 150398819

Following gene: 150398817

Centisome position: 3.4

GC content: 35.73

Gene sequence:

>1377_bases
ATGAATGATAAAAAATTTGCTGTAATAAATCCCTGTAAAATATGCCAGCCAATGGGGGCAATACTCGCATTACTTGGTGT
GAAAAATGCAATGCCGTTGATACACGGTTCTCACGGATGCAGCACGTACATGAGATTTCAACTCGGCCGTCACTTCAAAG
AACCTGTAAGCGTGTCGTCAACATCAATGAGTGAAGCAAACGCCGTACTCGGAGGTGAAGAAAACTTAAAGAATGCAATT
AAAAACGTCTGCAAAATTTATAAACCCGAAATAATAGGCATAACTACAAGCTGTCTTAGTGAAACTATTGGGGACGACGT
TGAAGGAATAATCCGGCTTTTTAAAACAAAAAATGAAGATTTGCCGTATTTAGTACCAATATCAACCCCAAGTTACAAAA
ATACCCATGTAGAAGGATACGATTCAACAGTTAAGGCACTTGTTTCGAATTTAGCAACTCCATCAAAATCAAACTCCAAA
TTAAATTTAATTACTGGAATGGTCTCACCAAGCGATACTGCAGAAATTAAACGGATATTACAGGAAATTGGTATTGATTA
CATAATTTTAACTGATACATCGGAGTCATTGGACGCCCCATTTGATGGAGACGTTTCATTCATGCCAAATGCCGGAACAA
CGGTAAACGAAATTAAAGATACTGCAAATTCTTATGCAACAGTTTCACTGTGTAAACATGCGAGTTCTGCTGCAAAATTC
CTTGAAGAAACTTATAAAATAAAATCAATTTCGACATACCTTCCAATTGGGCTACAAAATACTGATGAATTTTTAAATGA
AATTACTAAAATTTCAAATGTCGAAATCCCCAATAAGATTCAAAAAGAACGTGGAAAGTTGATTGATGCAATGATTGACG
CCCACCAGCATAATTTTGGACGAAAAGTTGCAATCTATGGCGACCCTGACATTGTAGTTGGATTTACAAGATTTGTAATG
GAACTTGGAATGATTCCTTCAATTGTCTGTACTGGTAACGAAAGTGAAGCGTTTATAAATGATATAAATGAAATAACAAA
AAACAGTAAGGATAAGCCAGTAATTCTTTCTGGAGGGGATTTATATGACCTTCATCAGGAAATAAAAAAATCAAAAGTGG
ATTTATTGATTGGAAATTCATATGGATCAAGAATTGCAGGTGCTGAAAATATACCCTTGATTAGAATGGGGTTCCCAATT
TACGATAGGGTTGGAGCTCAAAGAATAACCTGTTTAGGCTATTCAGCAGGAATTAAACTTGTGGATACAATAACAAATAC
AATTCTCGACAGGTACTTTGATGAGTTTGGATGGGCATTAGATGAAGATAAGGAGGTACAAAATGAAAACCTTAAAAATA
GCTGTTTCCTCAACTGA

Upstream 100 bases:

>100_bases
TTCAGGATATAACGGATTTATAAATTTTGCAAGAGTTGTGCATACGGCAGTTATGACGCCGGTTTGGAGACTTTCGAAGA
AAATGACGTAGAGGAATATT

Downstream 100 bases:

>100_bases
TGGCGTAAACATTAACATGCATTTTGGATCGTCTACTCATTTTCTTATTTTTGAAATAAACGATAAAATAGCAGAATTCC
TAGAGTTAAGGGAAAACCCT

Product: nitrogenase

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MNDKKFAVINPCKICQPMGAILALLGVKNAMPLIHGSHGCSTYMRFQLGRHFKEPVSVSSTSMSEANAVLGGEENLKNAI
KNVCKIYKPEIIGITTSCLSETIGDDVEGIIRLFKTKNEDLPYLVPISTPSYKNTHVEGYDSTVKALVSNLATPSKSNSK
LNLITGMVSPSDTAEIKRILQEIGIDYIILTDTSESLDAPFDGDVSFMPNAGTTVNEIKDTANSYATVSLCKHASSAAKF
LEETYKIKSISTYLPIGLQNTDEFLNEITKISNVEIPNKIQKERGKLIDAMIDAHQHNFGRKVAIYGDPDIVVGFTRFVM
ELGMIPSIVCTGNESEAFINDINEITKNSKDKPVILSGGDLYDLHQEIKKSKVDLLIGNSYGSRIAGAENIPLIRMGFPI
YDRVGAQRITCLGYSAGIKLVDTITNTILDRYFDEFGWALDEDKEVQNENLKNSCFLN

Sequences:

>Translated_458_residues
MNDKKFAVINPCKICQPMGAILALLGVKNAMPLIHGSHGCSTYMRFQLGRHFKEPVSVSSTSMSEANAVLGGEENLKNAI
KNVCKIYKPEIIGITTSCLSETIGDDVEGIIRLFKTKNEDLPYLVPISTPSYKNTHVEGYDSTVKALVSNLATPSKSNSK
LNLITGMVSPSDTAEIKRILQEIGIDYIILTDTSESLDAPFDGDVSFMPNAGTTVNEIKDTANSYATVSLCKHASSAAKF
LEETYKIKSISTYLPIGLQNTDEFLNEITKISNVEIPNKIQKERGKLIDAMIDAHQHNFGRKVAIYGDPDIVVGFTRFVM
ELGMIPSIVCTGNESEAFINDINEITKNSKDKPVILSGGDLYDLHQEIKKSKVDLLIGNSYGSRIAGAENIPLIRMGFPI
YDRVGAQRITCLGYSAGIKLVDTITNTILDRYFDEFGWALDEDKEVQNENLKNSCFLN
>Mature_458_residues
MNDKKFAVINPCKICQPMGAILALLGVKNAMPLIHGSHGCSTYMRFQLGRHFKEPVSVSSTSMSEANAVLGGEENLKNAI
KNVCKIYKPEIIGITTSCLSETIGDDVEGIIRLFKTKNEDLPYLVPISTPSYKNTHVEGYDSTVKALVSNLATPSKSNSK
LNLITGMVSPSDTAEIKRILQEIGIDYIILTDTSESLDAPFDGDVSFMPNAGTTVNEIKDTANSYATVSLCKHASSAAKF
LEETYKIKSISTYLPIGLQNTDEFLNEITKISNVEIPNKIQKERGKLIDAMIDAHQHNFGRKVAIYGDPDIVVGFTRFVM
ELGMIPSIVCTGNESEAFINDINEITKNSKDKPVILSGGDLYDLHQEIKKSKVDLLIGNSYGSRIAGAENIPLIRMGFPI
YDRVGAQRITCLGYSAGIKLVDTITNTILDRYFDEFGWALDEDKEVQNENLKNSCFLN

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 50362; Mature: 50362

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKKFAVINPCKICQPMGAILALLGVKNAMPLIHGSHGCSTYMRFQLGRHFKEPVSVSS
CCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHCCCCCCCC
TSMSEANAVLGGEENLKNAIKNVCKIYKPEIIGITTSCLSETIGDDVEGIIRLFKTKNED
CCHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCHHHHHHHHHHCCCCC
LPYLVPISTPSYKNTHVEGYDSTVKALVSNLATPSKSNSKLNLITGMVSPSDTAEIKRIL
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHH
QEIGIDYIILTDTSESLDAPFDGDVSFMPNAGTTVNEIKDTANSYATVSLCKHASSAAKF
HHCCCCEEEEECCCCCCCCCCCCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
LEETYKIKSISTYLPIGLQNTDEFLNEITKISNVEIPNKIQKERGKLIDAMIDAHQHNFG
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
RKVAIYGDPDIVVGFTRFVMELGMIPSIVCTGNESEAFINDINEITKNSKDKPVILSGGD
CEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEEECCC
LYDLHQEIKKSKVDLLIGNSYGSRIAGAENIPLIRMGFPIYDRVGAQRITCLGYSAGIKL
HHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEECCCHHHHCCCCEEEEEEHHCCCHH
VDTITNTILDRYFDEFGWALDEDKEVQNENLKNSCFLN
HHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNDKKFAVINPCKICQPMGAILALLGVKNAMPLIHGSHGCSTYMRFQLGRHFKEPVSVSS
CCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHCCCCCCCC
TSMSEANAVLGGEENLKNAIKNVCKIYKPEIIGITTSCLSETIGDDVEGIIRLFKTKNED
CCHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCHHHHHHHHHHCCCCC
LPYLVPISTPSYKNTHVEGYDSTVKALVSNLATPSKSNSKLNLITGMVSPSDTAEIKRIL
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHH
QEIGIDYIILTDTSESLDAPFDGDVSFMPNAGTTVNEIKDTANSYATVSLCKHASSAAKF
HHCCCCEEEEECCCCCCCCCCCCCCEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
LEETYKIKSISTYLPIGLQNTDEFLNEITKISNVEIPNKIQKERGKLIDAMIDAHQHNFG
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
RKVAIYGDPDIVVGFTRFVMELGMIPSIVCTGNESEAFINDINEITKNSKDKPVILSGGD
CEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEEECCC
LYDLHQEIKKSKVDLLIGNSYGSRIAGAENIPLIRMGFPIYDRVGAQRITCLGYSAGIKL
HHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEECCCHHHHCCCCEEEEEEHHCCCHH
VDTITNTILDRYFDEFGWALDEDKEVQNENLKNSCFLN
HHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550408; 7961410 [H]