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The map label for this gene is afr [H]

Identifier: 150398747

GI number: 150398747

Start: 3770980

End: 3772110

Strand: Reverse

Name: afr [H]

Synonym: Smed_3564

Alternate gene names: 150398747

Gene position: 3772110-3770980 (Counterclockwise)

Preceding gene: 150398749

Following gene: 150398746

Centisome position: 99.74

GC content: 63.66

Gene sequence:

>1131_bases
ATGAGCGGCAGGCGCAGGCTGGGCATCGGCCTGATCGGCACGGGCTTCATGGGCAAGGCCCATGCTCTCGGCTTCACGAT
TGCAGCGCGGGTCTTCGACCTGCCTTTCGAGCTGGACCTCGTATCCGTCGCGGACGTGACTGTGGAGGGCGCGGAGGCGG
CCAGGGGACGGCTCGGCTTCCGCAAGGCGACCACCGACTGGCGCGACCTTCTGATCGACCCGGAGATCGACGTCATCGAT
ATCACCACGCCGAACCTCCTGCACAAGGAGATGGCGCTCGCCGCGATTGCCCATGGTAAGCACGTCTATTGCGAAAAGCC
GCTGGCCCCGACGGTCGCCGACTGCGCCGAGATGGTTGCGGCGGCCGAAAAGGCAGGGGTCGTCACCCAGCTCGGCTTCA
ACTATCTCAAGAACCCGCTCATTTTTCTCGCCAGGGACATCATCGAAAGCGGCGAGATCGGCGAGATACGGTCGTTTCGC
GGCGTTCACGCGGAGGACTTCATGGCGGATCGGACCGTTCCCTGGGGCTGGCGGCTCGATCCGCGCAGCGGCGGCGGGGC
TCTTGCCGACATCGGCAGCCATATGATCGCCTGCATGCGCCATCTCGTCGGGCCCGTTAGGTCCGTGCTGGCCGACTCCG
TAATCCACGTTGCGGAACGCCCGCTCGCTCGCGGCGCAACAGAGACCCGCGCCGTCGAAGTCGACGACGTAACGCGGGCT
TTTGTTCGATTCGAGAGCGGCGCAAGCGGGAGCTTCGAAGCCAACTGGATCGCGACCGGCCGCAAGATGCAGCACGACTT
CGAAATTTACGGGTCAAAAGGCAGCATCGTCTTCACGCAGGAACGGCTGAACGAAATCAAGATCTATTATGCGGGCGACG
ATATAAGGAGCCGCGGCTTCCGCACCATCTGGGCGGGTCCGGAACATCCGCCCTACGGGGCGTTCTGCGTCGCTCCGGGC
CACCAGATCGGCTTCAACGATCTGAAGGCGATCGAGGTCCACGAATTCCTGGAAGCGATCGCGAATGGCGTCAGGACGTC
TACCGATTTCCGCGAGGGTTATGAGGTCCAGAAGGTCCTCTCCGCGACCTACCACTCCGCCCGGACGAACGCCTGGGTGG
AGATCGGGTGA

Upstream 100 bases:

>100_bases
AGAGAAATGTTTTCGAACAAATATTCCATATTGACATTGTTCGCACAGTTCATTTCATTTCCACCGAACGCGAATGAGGG
CCTGACCCGGGAGAAACGAC

Downstream 100 bases:

>100_bases
ATGGCGTGGCCGTTGGGCCCTTCTCCCGCGTTCAACATGGATAAGGTATCAAAATGGAATATCCATTCTATCCCGAGATA
GGCTAATCCATTAGAGTCGG

Product: oxidoreductase domain-containing protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSGRRRLGIGLIGTGFMGKAHALGFTIAARVFDLPFELDLVSVADVTVEGAEAARGRLGFRKATTDWRDLLIDPEIDVID
ITTPNLLHKEMALAAIAHGKHVYCEKPLAPTVADCAEMVAAAEKAGVVTQLGFNYLKNPLIFLARDIIESGEIGEIRSFR
GVHAEDFMADRTVPWGWRLDPRSGGGALADIGSHMIACMRHLVGPVRSVLADSVIHVAERPLARGATETRAVEVDDVTRA
FVRFESGASGSFEANWIATGRKMQHDFEIYGSKGSIVFTQERLNEIKIYYAGDDIRSRGFRTIWAGPEHPPYGAFCVAPG
HQIGFNDLKAIEVHEFLEAIANGVRTSTDFREGYEVQKVLSATYHSARTNAWVEIG

Sequences:

>Translated_376_residues
MSGRRRLGIGLIGTGFMGKAHALGFTIAARVFDLPFELDLVSVADVTVEGAEAARGRLGFRKATTDWRDLLIDPEIDVID
ITTPNLLHKEMALAAIAHGKHVYCEKPLAPTVADCAEMVAAAEKAGVVTQLGFNYLKNPLIFLARDIIESGEIGEIRSFR
GVHAEDFMADRTVPWGWRLDPRSGGGALADIGSHMIACMRHLVGPVRSVLADSVIHVAERPLARGATETRAVEVDDVTRA
FVRFESGASGSFEANWIATGRKMQHDFEIYGSKGSIVFTQERLNEIKIYYAGDDIRSRGFRTIWAGPEHPPYGAFCVAPG
HQIGFNDLKAIEVHEFLEAIANGVRTSTDFREGYEVQKVLSATYHSARTNAWVEIG
>Mature_375_residues
SGRRRLGIGLIGTGFMGKAHALGFTIAARVFDLPFELDLVSVADVTVEGAEAARGRLGFRKATTDWRDLLIDPEIDVIDI
TTPNLLHKEMALAAIAHGKHVYCEKPLAPTVADCAEMVAAAEKAGVVTQLGFNYLKNPLIFLARDIIESGEIGEIRSFRG
VHAEDFMADRTVPWGWRLDPRSGGGALADIGSHMIACMRHLVGPVRSVLADSVIHVAERPLARGATETRAVEVDDVTRAF
VRFESGASGSFEANWIATGRKMQHDFEIYGSKGSIVFTQERLNEIKIYYAGDDIRSRGFRTIWAGPEHPPYGAFCVAPGH
QIGFNDLKAIEVHEFLEAIANGVRTSTDFREGYEVQKVLSATYHSARTNAWVEIG

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI87082405, Length=285, Percent_Identity=27.3684210526316, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787574, Length=348, Percent_Identity=25.2873563218391, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081947, Length=223, Percent_Identity=27.3542600896861, Blast_Score=62, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323975, Length=281, Percent_Identity=25.9786476868327, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 41165; Mature: 41034

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRRRLGIGLIGTGFMGKAHALGFTIAARVFDLPFELDLVSVADVTVEGAEAARGRLGF
CCCCCEECEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHCCCCC
RKATTDWRDLLIDPEIDVIDITTPNLLHKEMALAAIAHGKHVYCEKPLAPTVADCAEMVA
HHCCCCHHHEEECCCCCEEECCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHH
AAEKAGVVTQLGFNYLKNPLIFLARDIIESGEIGEIRSFRGVHAEDFMADRTVPWGWRLD
HHHHCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCCCCCCEEEC
PRSGGGALADIGSHMIACMRHLVGPVRSVLADSVIHVAERPLARGATETRAVEVDDVTRA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHEEEEHHHHHHH
FVRFESGASGSFEANWIATGRKMQHDFEIYGSKGSIVFTQERLNEIKIYYAGDDIRSRGF
HHHCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCEEEECCCCCEEEEEECCCHHHHCCC
RTIWAGPEHPPYGAFCVAPGHQIGFNDLKAIEVHEFLEAIANGVRTSTDFREGYEVQKVL
EEEECCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
SATYHSARTNAWVEIG
HHHHHHHCCCEEEEEC
>Mature Secondary Structure 
SGRRRLGIGLIGTGFMGKAHALGFTIAARVFDLPFELDLVSVADVTVEGAEAARGRLGF
CCCCEECEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHCCCCC
RKATTDWRDLLIDPEIDVIDITTPNLLHKEMALAAIAHGKHVYCEKPLAPTVADCAEMVA
HHCCCCHHHEEECCCCCEEECCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHH
AAEKAGVVTQLGFNYLKNPLIFLARDIIESGEIGEIRSFRGVHAEDFMADRTVPWGWRLD
HHHHCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCCCCCCEEEC
PRSGGGALADIGSHMIACMRHLVGPVRSVLADSVIHVAERPLARGATETRAVEVDDVTRA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHEEEEHHHHHHH
FVRFESGASGSFEANWIATGRKMQHDFEIYGSKGSIVFTQERLNEIKIYYAGDDIRSRGF
HHHCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCEEEECCCCCEEEEEECCCHHHHCCC
RTIWAGPEHPPYGAFCVAPGHQIGFNDLKAIEVHEFLEAIANGVRTSTDFREGYEVQKVL
EEEECCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
SATYHSARTNAWVEIG
HHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA