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The map label for this gene is lepA [H]

Identifier: 150398655

GI number: 150398655

Start: 3676782

End: 3678617

Strand: Reverse

Name: lepA [H]

Synonym: Smed_3469

Alternate gene names: 150398655

Gene position: 3678617-3676782 (Counterclockwise)

Preceding gene: 150398656

Following gene: 150398653

Centisome position: 97.27

GC content: 59.8

Gene sequence:

>1836_bases
ATGCGGCCCATGAGCACATCATCCTCAAAGACGCCTCTGTCGCACATCCGCAACTTCTCGATCGTGGCCCATATCGACCA
CGGCAAGTCGACGCTGGCCGATCGGCTTATCCAATCGACCGGCGGCCTTGCCGAGCGCGAAATGTCCGAACAGGTGCTGG
ACAGCATGGATATCGAGCGCGAGCGCGGCATTACCATCAAGGCCCAGACCGTTCGCCTGCATTACAAGGCGAACAACGGC
GAGACCTATGTGCTGAACCTCATCGACACGCCCGGGCATGTCGACTTTGCCTATGAGGTCTCGCGCTCGCTGTCGGCCTG
CGAAGGTTCGCTGCTCGTCGTCGATGCCAGCCAGGGCGTCGAAGCACAGACGCTCGCCAATGTTTACCAGGCGATCGACA
ACAATCACGAACTCGTCACCGTACTCAACAAGATCGACCTTCCAGCGGCAGAGCCGGACCGGATCAAGGAGCAGATCGAG
GAAGTGATCGGCATCGACGCTTCCGAGGCCGTATTGATCTCGGCCAAGACCGGTCTCGGCATTCCGGATGTGCTGGAGGC
GATCGTCCATAAGCTTCCCGCCCCGAAGAGCGAAGGCGGCGACACCGCGCCCCTGAAGGCGCTTCTCGTGGACAGCTGGT
ACGACACCTATCTCGGCGTCATGGTCCTGGTACGCATCATCGACGGAACCCTGAAAAAGGGGATGACCATCCGCATGATG
GGGACGGATGCGAAATACCAGGTTGAACGCGTCGGCGTCCTCACGCCGAAAATGGTTGCCATGGATTCGCTCGGCCCCGG
CGAAATCGGATTCATCACCGCATCGATCAAGGAAGTGGCCGACACCCGCGTCGGCGACACGATCACTGAAGACAAGCGGC
CGACGGCTAAGGCGTTGCCGGGCTTCAAGCCGGCCCAGCCGGTGGTGTTCTGCGGCCTCTTCCCGGTCGACGCCGCCGAC
TTCGAGGACTTGCGTTCGGCCATGGGCAAGCTGCGTCTGAACGACGCATCCTTCTCCTTTGAGATGGAGTCCTCCGCCGC
ACTCGGCTTCGGCTTCCGCTGCGGCTTCCTCGGTCTGCTGCATCTGGAAATCATTCAGGAGCGCCTGGAGCGCGAATTCG
ACCTCGATCTGATCGCGACGGCGCCTTCGGTCGTCTACAAGCTGTTTATGACCGATGGCTCGGAGCGCGAGCTTCACAAC
CCCGCCGATATGCCGGATGTGGTCAAAATCGCTGAGATTCACGAGCCGTGGATCCGCGCGACGATCCTGACGCCGGACGA
ATATCTTGGTGGAATATTGAAGCTCTGTCAGGATCGCCGCGGCATCCAGATCGAACTGACCTATGTCGGCACCCGCGCAA
TGCTGACCTATGACTTGCCCTTGAACGAAGTCGTGTTCGATTTCTATGACCGGCTAAAGTCGATTTCCAAGGGTTATGCC
TCCTTCGACTACCAGATCACCGAGCACAAGGAAGGCAATCTCGTGAAGATGTCGATCCTCGTCAACGGCGAGCCGGTGGA
CGCGCTGTCGATGATGGTACACCGGATGGCGGCGGAAAAGCGCGGTCGCGAAATGTGCGAGAAGCTGAAGGAACTGATCC
CCAAGCACATGTTCAAGATTCCGATCCAGGCTGCCATCGGCGGCAATGTGATCGCCCGCGAAACCATCTCGGCGCTGCGC
AAGGACGTGACCGCGAAGTGCTACGGCGGCGACGCCACCCGCAAGCGCAAGCTTTTGGAAAAGCAGAAGGCCGGCAAGAA
GCGGATGCGCCAGTTTGGCAAGGTGGAAATCCCGCAGGAAGCTTTCATCGCGGCCTTGAAGATGAGCGACGAGTGA

Upstream 100 bases:

>100_bases
GGCGAGACCGAGGCACACGCGGAATGCCCGCCCCGGCGCCCGGATTTATAGTGAATGAAGCCTTCATTTCCCGCTTTGCG
CCGCCTGATCGCGCTGCTAT

Downstream 100 bases:

>100_bases
TTTTCCGGCGGCAGGATGCGCATATTCCATGCGCATCCTGCCGGAGCGAACATGCCACCATCGGCCGCAAGACGGCCCGC
CAATCTCTCCCGCGACAGTG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 611; Mature: 611

Protein sequence:

>611_residues
MRPMSTSSSKTPLSHIRNFSIVAHIDHGKSTLADRLIQSTGGLAEREMSEQVLDSMDIERERGITIKAQTVRLHYKANNG
ETYVLNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGVEAQTLANVYQAIDNNHELVTVLNKIDLPAAEPDRIKEQIE
EVIGIDASEAVLISAKTGLGIPDVLEAIVHKLPAPKSEGGDTAPLKALLVDSWYDTYLGVMVLVRIIDGTLKKGMTIRMM
GTDAKYQVERVGVLTPKMVAMDSLGPGEIGFITASIKEVADTRVGDTITEDKRPTAKALPGFKPAQPVVFCGLFPVDAAD
FEDLRSAMGKLRLNDASFSFEMESSAALGFGFRCGFLGLLHLEIIQERLEREFDLDLIATAPSVVYKLFMTDGSERELHN
PADMPDVVKIAEIHEPWIRATILTPDEYLGGILKLCQDRRGIQIELTYVGTRAMLTYDLPLNEVVFDFYDRLKSISKGYA
SFDYQITEHKEGNLVKMSILVNGEPVDALSMMVHRMAAEKRGREMCEKLKELIPKHMFKIPIQAAIGGNVIARETISALR
KDVTAKCYGGDATRKRKLLEKQKAGKKRMRQFGKVEIPQEAFIAALKMSDE

Sequences:

>Translated_611_residues
MRPMSTSSSKTPLSHIRNFSIVAHIDHGKSTLADRLIQSTGGLAEREMSEQVLDSMDIERERGITIKAQTVRLHYKANNG
ETYVLNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGVEAQTLANVYQAIDNNHELVTVLNKIDLPAAEPDRIKEQIE
EVIGIDASEAVLISAKTGLGIPDVLEAIVHKLPAPKSEGGDTAPLKALLVDSWYDTYLGVMVLVRIIDGTLKKGMTIRMM
GTDAKYQVERVGVLTPKMVAMDSLGPGEIGFITASIKEVADTRVGDTITEDKRPTAKALPGFKPAQPVVFCGLFPVDAAD
FEDLRSAMGKLRLNDASFSFEMESSAALGFGFRCGFLGLLHLEIIQERLEREFDLDLIATAPSVVYKLFMTDGSERELHN
PADMPDVVKIAEIHEPWIRATILTPDEYLGGILKLCQDRRGIQIELTYVGTRAMLTYDLPLNEVVFDFYDRLKSISKGYA
SFDYQITEHKEGNLVKMSILVNGEPVDALSMMVHRMAAEKRGREMCEKLKELIPKHMFKIPIQAAIGGNVIARETISALR
KDVTAKCYGGDATRKRKLLEKQKAGKKRMRQFGKVEIPQEAFIAALKMSDE
>Mature_611_residues
MRPMSTSSSKTPLSHIRNFSIVAHIDHGKSTLADRLIQSTGGLAEREMSEQVLDSMDIERERGITIKAQTVRLHYKANNG
ETYVLNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGVEAQTLANVYQAIDNNHELVTVLNKIDLPAAEPDRIKEQIE
EVIGIDASEAVLISAKTGLGIPDVLEAIVHKLPAPKSEGGDTAPLKALLVDSWYDTYLGVMVLVRIIDGTLKKGMTIRMM
GTDAKYQVERVGVLTPKMVAMDSLGPGEIGFITASIKEVADTRVGDTITEDKRPTAKALPGFKPAQPVVFCGLFPVDAAD
FEDLRSAMGKLRLNDASFSFEMESSAALGFGFRCGFLGLLHLEIIQERLEREFDLDLIATAPSVVYKLFMTDGSERELHN
PADMPDVVKIAEIHEPWIRATILTPDEYLGGILKLCQDRRGIQIELTYVGTRAMLTYDLPLNEVVFDFYDRLKSISKGYA
SFDYQITEHKEGNLVKMSILVNGEPVDALSMMVHRMAAEKRGREMCEKLKELIPKHMFKIPIQAAIGGNVIARETISALR
KDVTAKCYGGDATRKRKLLEKQKAGKKRMRQFGKVEIPQEAFIAALKMSDE

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=608, Percent_Identity=49.1776315789474, Blast_Score=611, Evalue=1e-175,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=48.5074626865672, Blast_Score=126, Evalue=7e-29,
Organism=Homo sapiens, GI18390331, Length=169, Percent_Identity=37.8698224852071, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI25306283, Length=182, Percent_Identity=40.6593406593407, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI19923640, Length=182, Percent_Identity=40.6593406593407, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=40, Blast_Score=107, Evalue=5e-23,
Organism=Homo sapiens, GI25306287, Length=182, Percent_Identity=40.6593406593407, Blast_Score=107, Evalue=5e-23,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=45.045045045045, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=45.045045045045, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=45.045045045045, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=33.0827067669173, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=33.0827067669173, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI94966752, Length=97, Percent_Identity=42.2680412371134, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI53729339, Length=247, Percent_Identity=27.1255060728745, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI53729337, Length=247, Percent_Identity=27.1255060728745, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=57.8859060402685, Blast_Score=707, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=512, Percent_Identity=26.5625, Blast_Score=159, Evalue=7e-40,
Organism=Escherichia coli, GI1789738, Length=161, Percent_Identity=34.7826086956522, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1790835, Length=171, Percent_Identity=30.4093567251462, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1789559, Length=240, Percent_Identity=30, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=620, Percent_Identity=38.8709677419355, Blast_Score=454, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71988811, Length=144, Percent_Identity=37.5, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71988819, Length=144, Percent_Identity=37.5, Blast_Score=105, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17556745, Length=171, Percent_Identity=35.672514619883, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17506493, Length=156, Percent_Identity=37.8205128205128, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=147, Percent_Identity=37.4149659863946, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=34.5864661654135, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71994658, Length=224, Percent_Identity=26.7857142857143, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=607, Percent_Identity=44.4810543657331, Blast_Score=539, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6324707, Length=146, Percent_Identity=39.041095890411, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=146, Percent_Identity=39.041095890411, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=221, Percent_Identity=33.0316742081448, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=121, Percent_Identity=38.8429752066116, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324166, Length=172, Percent_Identity=34.8837209302326, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI78706572, Length=605, Percent_Identity=44.9586776859504, Blast_Score=548, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24582462, Length=167, Percent_Identity=37.7245508982036, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=44.2028985507246, Blast_Score=106, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24585711, Length=148, Percent_Identity=36.4864864864865, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24585713, Length=148, Percent_Identity=36.4864864864865, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24585709, Length=148, Percent_Identity=36.4864864864865, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21357743, Length=162, Percent_Identity=33.9506172839506, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI221458488, Length=141, Percent_Identity=40.4255319148936, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19921738, Length=207, Percent_Identity=29.951690821256, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67546; Mature: 67546

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPMSTSSSKTPLSHIRNFSIVAHIDHGKSTLADRLIQSTGGLAEREMSEQVLDSMDIER
CCCCCCCCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHH
ERGITIKAQTVRLHYKANNGETYVLNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGV
HCCCEEEEEEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCC
EAQTLANVYQAIDNNHELVTVLNKIDLPAAEPDRIKEQIEEVIGIDASEAVLISAKTGLG
CHHHHHHHHHHHCCCCCEEEEEHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCC
IPDVLEAIVHKLPAPKSEGGDTAPLKALLVDSWYDTYLGVMVLVRIIDGTLKKGMTIRMM
CHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
GTDAKYQVERVGVLTPKMVAMDSLGPGEIGFITASIKEVADTRVGDTITEDKRPTAKALP
CCCCCEEEEEECCCCCCHHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHCCC
GFKPAQPVVFCGLFPVDAADFEDLRSAMGKLRLNDASFSFEMESSAALGFGFRCGFLGLL
CCCCCCCEEEEEECCCCCCCHHHHHHHHCCEEECCCCEEEEECCCCCCCCCHHHHHHHHH
HLEIIQERLEREFDLDLIATAPSVVYKLFMTDGSERELHNPADMPDVVKIAEIHEPWIRA
HHHHHHHHHHHHCCCCCEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHEE
TILTPDEYLGGILKLCQDRRGIQIELTYVGTRAMLTYDLPLNEVVFDFYDRLKSISKGYA
EEECCHHHHHHHHHHHHCCCCCEEEEEEEECEEEEEECCCHHHHHHHHHHHHHHHHCCCC
SFDYQITEHKEGNLVKMSILVNGEPVDALSMMVHRMAAEKRGREMCEKLKELIPKHMFKI
CCCEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PIQAAIGGNVIARETISALRKDVTAKCYGGDATRKRKLLEKQKAGKKRMRQFGKVEIPQE
CHHHHCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AFIAALKMSDE
HHHHHHHCCCC
>Mature Secondary Structure
MRPMSTSSSKTPLSHIRNFSIVAHIDHGKSTLADRLIQSTGGLAEREMSEQVLDSMDIER
CCCCCCCCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHH
ERGITIKAQTVRLHYKANNGETYVLNLIDTPGHVDFAYEVSRSLSACEGSLLVVDASQGV
HCCCEEEEEEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCC
EAQTLANVYQAIDNNHELVTVLNKIDLPAAEPDRIKEQIEEVIGIDASEAVLISAKTGLG
CHHHHHHHHHHHCCCCCEEEEEHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCC
IPDVLEAIVHKLPAPKSEGGDTAPLKALLVDSWYDTYLGVMVLVRIIDGTLKKGMTIRMM
CHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
GTDAKYQVERVGVLTPKMVAMDSLGPGEIGFITASIKEVADTRVGDTITEDKRPTAKALP
CCCCCEEEEEECCCCCCHHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHCCC
GFKPAQPVVFCGLFPVDAADFEDLRSAMGKLRLNDASFSFEMESSAALGFGFRCGFLGLL
CCCCCCCEEEEEECCCCCCCHHHHHHHHCCEEECCCCEEEEECCCCCCCCCHHHHHHHHH
HLEIIQERLEREFDLDLIATAPSVVYKLFMTDGSERELHNPADMPDVVKIAEIHEPWIRA
HHHHHHHHHHHHCCCCCEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHEE
TILTPDEYLGGILKLCQDRRGIQIELTYVGTRAMLTYDLPLNEVVFDFYDRLKSISKGYA
EEECCHHHHHHHHHHHHCCCCCEEEEEEEECEEEEEECCCHHHHHHHHHHHHHHHHCCCC
SFDYQITEHKEGNLVKMSILVNGEPVDALSMMVHRMAAEKRGREMCEKLKELIPKHMFKI
CCCEEEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PIQAAIGGNVIARETISALRKDVTAKCYGGDATRKRKLLEKQKAGKKRMRQFGKVEIPQE
CHHHHCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AFIAALKMSDE
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA