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The map label for this gene is pit [H]

Identifier: 150398582

GI number: 150398582

Start: 3598801

End: 3599805

Strand: Reverse

Name: pit [H]

Synonym: Smed_3393

Alternate gene names: 150398582

Gene position: 3599805-3598801 (Counterclockwise)

Preceding gene: 150398583

Following gene: 150398581

Centisome position: 95.18

GC content: 61.79

Gene sequence:

>1005_bases
ATGGATGCGACGCTCGCCTTCCCCCTGCTTGTGGGGCTCATCGCCGTCGCGCTGTTCTTCGATTTCCTCAACGGATTGCA
CGACGCGGCCAATTCGATCGCGACCATCGTTTCGACCCGCGTGCTCCGGCCGCAATATGCCGTCTTATGGGCGGCGTTCT
TCAACTTCATCGCCTTCCTCTTCTTCGGGCTTCACGTTGCCGAAACGCTTGGCACCGGCATCATCGATCCGGGTATCGTT
ACGCCACAGGTCATTTTCGCGGCCTTGATGGGCGCGATCATCTGGAACATCGTTACCTGGATTTTCGGAATTCCGTCGAG
TTCCTCACACGCTCTGATCGGCGGCCTCGTCGGCGCCGGCCTCGCCAAGACGGGCTTCAGTTCGATCGTCTGGCAGGGCC
TCCTGAAGACGGCCGGGGCCATCGTCATGTCGCCGGGAATCGGCTTCGTTCTGGCGCTGCTCCTGGTGCTGATCGTCTCC
TGGCTCTTCGTTCGCCAGACACCTTTCGCCGTCGACAGCACCTTCCGGGTGCTGCAGTTCGTTTCCGCTTCATTTTACTC
GCTCGGCCACGGCGGCAATGATGCACAGAAAACCATGGGCATCATTGCCGTGCTTCTTTTCTCGCAAGGCTATCTCGGTT
CGGATTTCTACGTGCCTTTCTGGGTGGTCATCACCTGCCAGGCGGCGATCGCACTCGGCACGCTCTTCGGCGGCTGGAGG
ATCGTACACACGATGGGCTCGAAGATAACCAAGCTCAATCCGATGCAGGGTTTCTGCGCCGAGACGGGCGGTGCCATTAC
CCTGTTCGCCGCGACCTGGCTCGGAATTCCGGTCTCGACAACCCACACGATCACCGGCGCGATCATCGGCGTCGGCGCGG
CGCGGCGCGTGTCGGCGGTGCGCTGGGGCCTTGCCGGCAACATCGTCGTCGCCTGGGTGATTACGCTTCCCGCCGCCGCC
CTGATCTCCGCCCTTTGCTATTTCGCCGTCGGTCTGGTCGGCTGA

Upstream 100 bases:

>100_bases
ATATCATCGGCAGCGAGATCTATGGCGAACTGGAAAAGGTCGTCGACCGCTTTGAGGACGTTGCAAACGAAATCAGCGGC
ATCGTGATCGAGAACGTCTG

Downstream 100 bases:

>100_bases
TCCTTCCTTCGTCAGGTGGCCCGTTGGCATCGCCGGCGGTAGGGAGTATATTTTCACCACCTAATAGAATCGCATCCGAA
GGAAAATGAACTCCATCGTA

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MDATLAFPLLVGLIAVALFFDFLNGLHDAANSIATIVSTRVLRPQYAVLWAAFFNFIAFLFFGLHVAETLGTGIIDPGIV
TPQVIFAALMGAIIWNIVTWIFGIPSSSSHALIGGLVGAGLAKTGFSSIVWQGLLKTAGAIVMSPGIGFVLALLLVLIVS
WLFVRQTPFAVDSTFRVLQFVSASFYSLGHGGNDAQKTMGIIAVLLFSQGYLGSDFYVPFWVVITCQAAIALGTLFGGWR
IVHTMGSKITKLNPMQGFCAETGGAITLFAATWLGIPVSTTHTITGAIIGVGAARRVSAVRWGLAGNIVVAWVITLPAAA
LISALCYFAVGLVG

Sequences:

>Translated_334_residues
MDATLAFPLLVGLIAVALFFDFLNGLHDAANSIATIVSTRVLRPQYAVLWAAFFNFIAFLFFGLHVAETLGTGIIDPGIV
TPQVIFAALMGAIIWNIVTWIFGIPSSSSHALIGGLVGAGLAKTGFSSIVWQGLLKTAGAIVMSPGIGFVLALLLVLIVS
WLFVRQTPFAVDSTFRVLQFVSASFYSLGHGGNDAQKTMGIIAVLLFSQGYLGSDFYVPFWVVITCQAAIALGTLFGGWR
IVHTMGSKITKLNPMQGFCAETGGAITLFAATWLGIPVSTTHTITGAIIGVGAARRVSAVRWGLAGNIVVAWVITLPAAA
LISALCYFAVGLVG
>Mature_334_residues
MDATLAFPLLVGLIAVALFFDFLNGLHDAANSIATIVSTRVLRPQYAVLWAAFFNFIAFLFFGLHVAETLGTGIIDPGIV
TPQVIFAALMGAIIWNIVTWIFGIPSSSSHALIGGLVGAGLAKTGFSSIVWQGLLKTAGAIVMSPGIGFVLALLLVLIVS
WLFVRQTPFAVDSTFRVLQFVSASFYSLGHGGNDAQKTMGIIAVLLFSQGYLGSDFYVPFWVVITCQAAIALGTLFGGWR
IVHTMGSKITKLNPMQGFCAETGGAITLFAATWLGIPVSTTHTITGAIIGVGAARRVSAVRWGLAGNIVVAWVITLPAAA
LISALCYFAVGLVG

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=161, Percent_Identity=35.4037267080745, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI5803173, Length=152, Percent_Identity=38.1578947368421, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1789907, Length=220, Percent_Identity=34.5454545454545, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789360, Length=223, Percent_Identity=33.1838565022422, Blast_Score=104, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI25146401, Length=170, Percent_Identity=33.5294117647059, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17539280, Length=151, Percent_Identity=33.112582781457, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71981576, Length=158, Percent_Identity=31.6455696202532, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI32566716, Length=173, Percent_Identity=32.3699421965318, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17536725, Length=172, Percent_Identity=30.2325581395349, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17557328, Length=164, Percent_Identity=29.2682926829268, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319773, Length=158, Percent_Identity=32.2784810126582, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21356511, Length=202, Percent_Identity=31.1881188118812, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35115; Mature: 35115

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]