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The map label for this gene is gidB

Identifier: 150398395

GI number: 150398395

Start: 3377223

End: 3377864

Strand: Reverse

Name: gidB

Synonym: Smed_3203

Alternate gene names: 150398395

Gene position: 3377864-3377223 (Counterclockwise)

Preceding gene: 150398396

Following gene: 150398394

Centisome position: 89.32

GC content: 54.67

Gene sequence:

>642_bases
ATGCAAACGAGTCTCAATCGAGCGCTGAACGGATTGCGTGTTTCACGTGAAACCGTCGAGAAACTGGAACACTTCGCTTC
GCTCTTCCAGAAGTGGGCGCGGTCGATCAACCTCGTCGCCCCTTCCACATTGGACGATCTATGGCGCCGCCATATTCTCG
ATAGCATTCAGCTTTTTCAACTCTCCCCTGCGCCCAAGACCTGGGTCGATCTCGGAAGCGGCGGCGGCTTCCCAGGTGTG
ATCACTGCGATTTGCCTCTCCGAGACAGAGTCTGGCTGGGTGCATTTGGTCGAAAGCAACAACAAGAAAGCCGCCTTTCT
GCGAGTCGCGCTTCGCGAGACCGCTGCAAGGGGGACCGTCCATCCGATTCGAATCGAAGAGGCGCCCGCTGAAATTCCCA
CTTGCGACGCCATATCGGCGCGTGCGCTCTCCGATTTAAGCCAGTTACTGGAATATTGCGCACCCTGGATGACAGTCGAA
GCATCCCGAACCATCGCGTTTTTTCATAAAGGGCGGGATTATCAACTTGAAATCGATAAAGCCGTTAGCCGCTTTCAATT
CGATCTGGTAAAACACGCAAGTGTCGTCGAGCCGGATTCAGTCGTGCTCGAGGTCGCAAATCTTTCACGTCGGACGAAGT
GA

Upstream 100 bases:

>100_bases
TGAAGGTCGACGGCGTGACACCCGCGGCCATTTCACTGATCCTGTCATGGCTGCGACGAGAAGACCGCCGTTCGAAGCGT
GTAGGGGGTTGAGTTCTGCA

Downstream 100 bases:

>100_bases
TAGGCGAGCGAAACATGTTTGGCCCAAAGAACCGGATCATCACCATTGCCAACCAGAAAGGTGGCGTCGGCAAGACGACC
ACTGCAATTAATCTTGCGAC

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MQTSLNRALNGLRVSRETVEKLEHFASLFQKWARSINLVAPSTLDDLWRRHILDSIQLFQLSPAPKTWVDLGSGGGFPGV
ITAICLSETESGWVHLVESNNKKAAFLRVALRETAARGTVHPIRIEEAPAEIPTCDAISARALSDLSQLLEYCAPWMTVE
ASRTIAFFHKGRDYQLEIDKAVSRFQFDLVKHASVVEPDSVVLEVANLSRRTK

Sequences:

>Translated_213_residues
MQTSLNRALNGLRVSRETVEKLEHFASLFQKWARSINLVAPSTLDDLWRRHILDSIQLFQLSPAPKTWVDLGSGGGFPGV
ITAICLSETESGWVHLVESNNKKAAFLRVALRETAARGTVHPIRIEEAPAEIPTCDAISARALSDLSQLLEYCAPWMTVE
ASRTIAFFHKGRDYQLEIDKAVSRFQFDLVKHASVVEPDSVVLEVANLSRRTK
>Mature_213_residues
MQTSLNRALNGLRVSRETVEKLEHFASLFQKWARSINLVAPSTLDDLWRRHILDSIQLFQLSPAPKTWVDLGSGGGFPGV
ITAICLSETESGWVHLVESNNKKAAFLRVALRETAARGTVHPIRIEEAPAEIPTCDAISARALSDLSQLLEYCAPWMTVE
ASRTIAFFHKGRDYQLEIDKAVSRFQFDLVKHASVVEPDSVVLEVANLSRRTK

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=149, Percent_Identity=28.1879194630872, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_SINMW (A6UEE7)

Other databases:

- EMBL:   CP000738
- RefSeq:   YP_001328862.1
- ProteinModelPortal:   A6UEE7
- SMR:   A6UEE7
- STRING:   A6UEE7
- GeneID:   5324082
- GenomeReviews:   CP000738_GR
- KEGG:   smd:Smed_3203
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   GGQGWIV
- ProtClustDB:   PRK00107
- BioCyc:   SMED366394:SMED_3203-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 23781; Mature: 23781

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTSLNRALNGLRVSRETVEKLEHFASLFQKWARSINLVAPSTLDDLWRRHILDSIQLFQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEE
LSPAPKTWVDLGSGGGFPGVITAICLSETESGWVHLVESNNKKAAFLRVALRETAARGTV
ECCCCCHHEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCC
HPIRIEEAPAEIPTCDAISARALSDLSQLLEYCAPWMTVEASRTIAFFHKGRDYQLEIDK
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCEEEEHHH
AVSRFQFDLVKHASVVEPDSVVLEVANLSRRTK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQTSLNRALNGLRVSRETVEKLEHFASLFQKWARSINLVAPSTLDDLWRRHILDSIQLFQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEE
LSPAPKTWVDLGSGGGFPGVITAICLSETESGWVHLVESNNKKAAFLRVALRETAARGTV
ECCCCCHHEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCC
HPIRIEEAPAEIPTCDAISARALSDLSQLLEYCAPWMTVEASRTIAFFHKGRDYQLEIDK
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCCEEEEHHH
AVSRFQFDLVKHASVVEPDSVVLEVANLSRRTK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA