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The map label for this gene is purR [H]

Identifier: 150398367

GI number: 150398367

Start: 3338189

End: 3339232

Strand: Reverse

Name: purR [H]

Synonym: Smed_3175

Alternate gene names: 150398367

Gene position: 3339232-3338189 (Counterclockwise)

Preceding gene: 150398369

Following gene: 150398366

Centisome position: 88.29

GC content: 65.04

Gene sequence:

>1044_bases
ATGGGAAAGGAGCGGGTCACGGTTGTCGACATTGCGCGTGCCGCCGGCGTGTCGAAATCGACCGTGTCGCTCGTCCTGCA
GGGAAGTCCGCTCGTCAATGAAGCGACGCGGGCAAAGGTCAATGCGGCGATCCGCGAGCTTGGCTATGTCTATAATCGCA
GCGCCGCCAATCTGCGCCAGGCGAGATCGAAGATCATCGGCATCGTCGTCAACGATCTCACGAACAGTTTCTTTGCGGAG
CTCGCGGTCGGCGTCGACGAGGTCGTGCAGTCCGCCGGCTTCGTGCAGTTTCTCGCCAATACCAGCGAGAAGCTGGAGCG
CCAGCGTGAGGTCATCGCCTCTATGCGCGAGCACGGCGTGTCGGGCCTCATCATATCGCCGGCGCGCGGCACGGAAGTCG
GCGATCTCGCACCGCTCGGCGCCGCCGGGATTCCGGTTGTACTCGTCGTGCGCGACATTCCCGGAGCCGATGTGTCGTCG
CTGATATCAGACAATCACGCTGGCGCCGTTGCCGCCGTGCGGCATCTGGTCGAGCGCGGCCATCGCCGCGTGGCCTTCCT
GGGCGGCTTTGCCGATACAGCGGTTTTCGAAGCGCGAATGCGCGGCTATCGCGATGCCTTGCAAGAGGCGGGTCTGGATA
CCTCCGAGACTCTGACCATCGGCTCGGCGCCGTCTCGCGCCGGCGGTGTCACGGCAATAGAGCAGGCGATGATGCTGAAG
GAGCGGCCGACCGCGGCGCTCTGCTTCAACGATGCCGTCGCCTTCGGTGTCTGCGACGGCTTGCGCGCGCGTCGGCTGGA
GCCCGGCGAAGACTTCGCGGTGATCGGTTTCGACGACGTGATCGAAGCGAAAACCGCGGTGCCGGCGCTGACCACGGTCT
CTGTCGATCCGCAGGGCATGGGCGAGCGGGCGGCGGAGCTGCTTCTGAAACAGATCAATAGCGGCCGTGTCGAGCCGGAA
GCGATCGTCAGCCCGGTACGGCTCGCCATCCGCCAGAGCTGCGGCGCAGCCGTCGGACGCAGGATGAAGGAGATTTCGTC
ATGA

Upstream 100 bases:

>100_bases
GGCAAGACGCTGAGAAAAAATGAAAGCAGGCCTCTTGACGAAATTGGAACGATCCAATTAATAAGGATGCGAGAGGCGCA
AAGCCAGTTGGAGGATGTCC

Downstream 100 bases:

>100_bases
TTCGCTGGGGATTGATCGGTGCGAGCACCATTGCGCGTGAATGGGTGATCGGCGCCATCCGCGCATCAGGTGGCGAGGTC
GTGTCGGTGATGAGTTCGAG

Product: periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MGKERVTVVDIARAAGVSKSTVSLVLQGSPLVNEATRAKVNAAIRELGYVYNRSAANLRQARSKIIGIVVNDLTNSFFAE
LAVGVDEVVQSAGFVQFLANTSEKLERQREVIASMREHGVSGLIISPARGTEVGDLAPLGAAGIPVVLVVRDIPGADVSS
LISDNHAGAVAAVRHLVERGHRRVAFLGGFADTAVFEARMRGYRDALQEAGLDTSETLTIGSAPSRAGGVTAIEQAMMLK
ERPTAALCFNDAVAFGVCDGLRARRLEPGEDFAVIGFDDVIEAKTAVPALTTVSVDPQGMGERAAELLLKQINSGRVEPE
AIVSPVRLAIRQSCGAAVGRRMKEISS

Sequences:

>Translated_347_residues
MGKERVTVVDIARAAGVSKSTVSLVLQGSPLVNEATRAKVNAAIRELGYVYNRSAANLRQARSKIIGIVVNDLTNSFFAE
LAVGVDEVVQSAGFVQFLANTSEKLERQREVIASMREHGVSGLIISPARGTEVGDLAPLGAAGIPVVLVVRDIPGADVSS
LISDNHAGAVAAVRHLVERGHRRVAFLGGFADTAVFEARMRGYRDALQEAGLDTSETLTIGSAPSRAGGVTAIEQAMMLK
ERPTAALCFNDAVAFGVCDGLRARRLEPGEDFAVIGFDDVIEAKTAVPALTTVSVDPQGMGERAAELLLKQINSGRVEPE
AIVSPVRLAIRQSCGAAVGRRMKEISS
>Mature_346_residues
GKERVTVVDIARAAGVSKSTVSLVLQGSPLVNEATRAKVNAAIRELGYVYNRSAANLRQARSKIIGIVVNDLTNSFFAEL
AVGVDEVVQSAGFVQFLANTSEKLERQREVIASMREHGVSGLIISPARGTEVGDLAPLGAAGIPVVLVVRDIPGADVSSL
ISDNHAGAVAAVRHLVERGHRRVAFLGGFADTAVFEARMRGYRDALQEAGLDTSETLTIGSAPSRAGGVTAIEQAMMLKE
RPTAALCFNDAVAFGVCDGLRARRLEPGEDFAVIGFDDVIEAKTAVPALTTVSVDPQGMGERAAELLLKQINSGRVEPEA
IVSPVRLAIRQSCGAAVGRRMKEISS

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=288, Percent_Identity=33.3333333333333, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=32.3170731707317, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=31.5315315315315, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1787906, Length=341, Percent_Identity=31.3782991202346, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788474, Length=320, Percent_Identity=32.8125, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI48994940, Length=323, Percent_Identity=31.8885448916409, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790369, Length=317, Percent_Identity=26.1829652996845, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1789068, Length=322, Percent_Identity=29.1925465838509, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790715, Length=326, Percent_Identity=26.3803680981595, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786540, Length=334, Percent_Identity=27.5449101796407, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787580, Length=334, Percent_Identity=26.3473053892216, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790689, Length=339, Percent_Identity=26.5486725663717, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786268, Length=312, Percent_Identity=24.3589743589744, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36568; Mature: 36437

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKERVTVVDIARAAGVSKSTVSLVLQGSPLVNEATRAKVNAAIRELGYVYNRSAANLRQ
CCCCCEEHEEHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ARSKIIGIVVNDLTNSFFAELAVGVDEVVQSAGFVQFLANTSEKLERQREVIASMREHGV
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHCCC
SGLIISPARGTEVGDLAPLGAAGIPVVLVVRDIPGADVSSLISDNHAGAVAAVRHLVERG
CEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHCC
HRRVAFLGGFADTAVFEARMRGYRDALQEAGLDTSETLTIGSAPSRAGGVTAIEQAMMLK
CCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHH
ERPTAALCFNDAVAFGVCDGLRARRLEPGEDFAVIGFDDVIEAKTAVPALTTVSVDPQGM
CCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHCCEEEEEECCCCCC
GERAAELLLKQINSGRVEPEAIVSPVRLAIRQSCGAAVGRRMKEISS
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GKERVTVVDIARAAGVSKSTVSLVLQGSPLVNEATRAKVNAAIRELGYVYNRSAANLRQ
CCCCEEHEEHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ARSKIIGIVVNDLTNSFFAELAVGVDEVVQSAGFVQFLANTSEKLERQREVIASMREHGV
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHHHHCCC
SGLIISPARGTEVGDLAPLGAAGIPVVLVVRDIPGADVSSLISDNHAGAVAAVRHLVERG
CEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHCC
HRRVAFLGGFADTAVFEARMRGYRDALQEAGLDTSETLTIGSAPSRAGGVTAIEQAMMLK
CCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHH
ERPTAALCFNDAVAFGVCDGLRARRLEPGEDFAVIGFDDVIEAKTAVPALTTVSVDPQGM
CCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHCCEEEEEECCCCCC
GERAAELLLKQINSGRVEPEAIVSPVRLAIRQSCGAAVGRRMKEISS
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA