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The map label for this gene is afr [H]

Identifier: 150398366

GI number: 150398366

Start: 3337191

End: 3338192

Strand: Reverse

Name: afr [H]

Synonym: Smed_3174

Alternate gene names: 150398366

Gene position: 3338192-3337191 (Counterclockwise)

Preceding gene: 150398367

Following gene: 150398365

Centisome position: 88.27

GC content: 64.57

Gene sequence:

>1002_bases
ATGATTCGCTGGGGATTGATCGGTGCGAGCACCATTGCGCGTGAATGGGTGATCGGCGCCATCCGCGCATCAGGTGGCGA
GGTCGTGTCGGTGATGAGTTCGAGCGCGGAGCGGGGACGGGCTTACGCTGCGGAAAACAGCATTGGAAGATCCGTGACGA
GCCTGGACGAGCTTGTCGGCGACCAGGACGTGGATGCCGTCTACATCTCGACCACAAATGAGCTTCACCACGGACAGGCG
CTTGCGGCAATCCGCGCCGGCAAGCACGTGCTCTGCGAGAAGCCCTTGGCGATGAGCCTCAACGACGGCTGCGAAATGGT
CCTCAAGGCCTGCGAGGCCGGTGTCGTGCTCGGCACGAACCATCACTTGCGCAATGCCGCCACGCATCGGGCGATGCGGG
AGGCGATCGCGGCGGGCAAGATCGGCAGGCCGATCGCAGCGCGGGTATTCCATGCCGTCCATCTGCCGCCGCACCTGCAG
GGGTGGCGGCTCGACAAGCCCGAGGCCGGAGGCGGTGTCATCCTCGACATTACGGTCCACGATGCCGATACGCTGCGCTT
CGTGCTGGACGACGATCCGATCGAGGCCGTCGCGATCAGCCACAGTGCCGGAATGGGTAAGGAGGGGCTCGAGGACGGCG
TCATGGGTGTGCTTCGTTTCCGTTCCGGCGTCATTGCCCAGTTCCACGACGCCTTCACCACGAAATATGCCGAGACGGGG
CTTGAAGTGCATGGAACCGCAGGATCGCTTATCGGCCGCAATGTGATGACGCAGCGACCCGTCGGTACGGTCGTGCTCCG
CAATGAGGAAGGCGAGAGCGAGCTGCCGCTCGACCATCGCAATCTCTACGAGACGGCGCTTTCCGCCTTCCATTCCGCGA
TCGAGGGTCAGGGCCGTCCGTCGGCCAGCGGCGAGGATGGGGTCTGGTCGCTCGCCACCGGTCTGGCGGTAGTCAAAGCG
GCGGCGACTGGCGCGGCCGTCGAGATCGAAACGGGACTCTGA

Upstream 100 bases:

>100_bases
GCGGCCGTGTCGAGCCGGAAGCGATCGTCAGCCCGGTACGGCTCGCCATCCGCCAGAGCTGCGGCGCAGCCGTCGGACGC
AGGATGAAGGAGATTTCGTC

Downstream 100 bases:

>100_bases
CGCGATGACGACGGGCAAACACATCTCGCCTGCGCAAGCAGCCGGTCTGATCCCCGACGGGGCGATCGTTTCGGTTTCCT
CCTCCAGCGGCCTTGGCTGC

Product: oxidoreductase domain-containing protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MIRWGLIGASTIAREWVIGAIRASGGEVVSVMSSSAERGRAYAAENSIGRSVTSLDELVGDQDVDAVYISTTNELHHGQA
LAAIRAGKHVLCEKPLAMSLNDGCEMVLKACEAGVVLGTNHHLRNAATHRAMREAIAAGKIGRPIAARVFHAVHLPPHLQ
GWRLDKPEAGGGVILDITVHDADTLRFVLDDDPIEAVAISHSAGMGKEGLEDGVMGVLRFRSGVIAQFHDAFTTKYAETG
LEVHGTAGSLIGRNVMTQRPVGTVVLRNEEGESELPLDHRNLYETALSAFHSAIEGQGRPSASGEDGVWSLATGLAVVKA
AATGAAVEIETGL

Sequences:

>Translated_333_residues
MIRWGLIGASTIAREWVIGAIRASGGEVVSVMSSSAERGRAYAAENSIGRSVTSLDELVGDQDVDAVYISTTNELHHGQA
LAAIRAGKHVLCEKPLAMSLNDGCEMVLKACEAGVVLGTNHHLRNAATHRAMREAIAAGKIGRPIAARVFHAVHLPPHLQ
GWRLDKPEAGGGVILDITVHDADTLRFVLDDDPIEAVAISHSAGMGKEGLEDGVMGVLRFRSGVIAQFHDAFTTKYAETG
LEVHGTAGSLIGRNVMTQRPVGTVVLRNEEGESELPLDHRNLYETALSAFHSAIEGQGRPSASGEDGVWSLATGLAVVKA
AATGAAVEIETGL
>Mature_333_residues
MIRWGLIGASTIAREWVIGAIRASGGEVVSVMSSSAERGRAYAAENSIGRSVTSLDELVGDQDVDAVYISTTNELHHGQA
LAAIRAGKHVLCEKPLAMSLNDGCEMVLKACEAGVVLGTNHHLRNAATHRAMREAIAAGKIGRPIAARVFHAVHLPPHLQ
GWRLDKPEAGGGVILDITVHDADTLRFVLDDDPIEAVAISHSAGMGKEGLEDGVMGVLRFRSGVIAQFHDAFTTKYAETG
LEVHGTAGSLIGRNVMTQRPVGTVVLRNEEGESELPLDHRNLYETALSAFHSAIEGQGRPSASGEDGVWSLATGLAVVKA
AATGAAVEIETGL

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=253, Percent_Identity=26.8774703557312, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1787574, Length=287, Percent_Identity=26.1324041811847, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87082405, Length=266, Percent_Identity=28.9473684210526, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI145693182, Length=180, Percent_Identity=28.3333333333333, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87081947, Length=183, Percent_Identity=28.9617486338798, Blast_Score=65, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24581117, Length=253, Percent_Identity=29.6442687747036, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24581115, Length=196, Percent_Identity=27.5510204081633, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 35066; Mature: 35066

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRWGLIGASTIAREWVIGAIRASGGEVVSVMSSSAERGRAYAAENSIGRSVTSLDELVG
CEEEEECCHHHHHHHHHHHEEECCCCCEEHHHHCCHHHCCHHHHHHHHCHHHHHHHHHHC
DQDVDAVYISTTNELHHGQALAAIRAGKHVLCEKPLAMSLNDGCEMVLKACEAGVVLGTN
CCCCCEEEEEECCCHHCCHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHCCEEECCC
HHLRNAATHRAMREAIAAGKIGRPIAARVFHAVHLPPHLQGWRLDKPEAGGGVILDITVH
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEE
DADTLRFVLDDDPIEAVAISHSAGMGKEGLEDGVMGVLRFRSGVIAQFHDAFTTKYAETG
CCCEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
LEVHGTAGSLIGRNVMTQRPVGTVVLRNEEGESELPLDHRNLYETALSAFHSAIEGQGRP
CEEECCCHHHHHCCHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
SASGEDGVWSLATGLAVVKAAATGAAVEIETGL
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC
>Mature Secondary Structure
MIRWGLIGASTIAREWVIGAIRASGGEVVSVMSSSAERGRAYAAENSIGRSVTSLDELVG
CEEEEECCHHHHHHHHHHHEEECCCCCEEHHHHCCHHHCCHHHHHHHHCHHHHHHHHHHC
DQDVDAVYISTTNELHHGQALAAIRAGKHVLCEKPLAMSLNDGCEMVLKACEAGVVLGTN
CCCCCEEEEEECCCHHCCHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHCCEEECCC
HHLRNAATHRAMREAIAAGKIGRPIAARVFHAVHLPPHLQGWRLDKPEAGGGVILDITVH
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEE
DADTLRFVLDDDPIEAVAISHSAGMGKEGLEDGVMGVLRFRSGVIAQFHDAFTTKYAETG
CCCEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
LEVHGTAGSLIGRNVMTQRPVGTVVLRNEEGESELPLDHRNLYETALSAFHSAIEGQGRP
CEEECCCHHHHHCCHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
SASGEDGVWSLATGLAVVKAAATGAAVEIETGL
CCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]