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The map label for this gene is gcvA [H]

Identifier: 150398348

GI number: 150398348

Start: 3314459

End: 3315352

Strand: Reverse

Name: gcvA [H]

Synonym: Smed_3156

Alternate gene names: 150398348

Gene position: 3315352-3314459 (Counterclockwise)

Preceding gene: 150398353

Following gene: 150398347

Centisome position: 87.66

GC content: 63.31

Gene sequence:

>894_bases
ATGAAGTTGAGCCGACGACTGGTTCCCGACGTGACCACGCTGCAGGCCTTCGAATGCGCGGCTCGCCATGGCAGCTTCAC
CCAGGCCGCCGCCGAGCTCAATCTCACGCAGAGTGCCGTCAGCCGGCAGATCAAGGATCTCGAGAGCCAGCTCGGGGTGC
TGCTCTTCGAGCGGGTGCGCCAGCGGGTCATTCTATCGGATGCGGGACAGAAATTCCTGCCCGAGGTGCGCCGGCTCCTG
AACCAGACCGAGGAGCTGATGGTACGCGCCATGGCATCGGCGCGGGCCGATTCGAGCCTTTCCATCGCATCGCTTCCGAC
TTTCGGGAGCCGCTGGCTGGTACAGCGCCTGCCCGACTTCCTGAAGCGGCACCCCGGTACCGTGGTCAACATCGCCTCCC
GCTCGGAGCCATTCGACTTCGAGGAGCAGAACTTCGACCTCGCCATCCATTATGGCCAGCCCGTCTGGGCGCGCGCCATC
TGCAGCTATCTCTGCAGCGAGGTCATCGTACCGGCGGCCAGCCCGGCGCTGCTTGCCGTGCATCCGGTCGCATCGCCTGC
GGATATCGGCGGCAGTCCGCTGCTGCATCTGGCGACGCGGCCCAAGCTTTGGGCGCAATGGTTCGAGACAAACGACGTCG
AGCAGGACGGCGCCTATCGAGGCAACCGCTTCGATCAATTCTCGATGGTGATCGAGGCGGCGACTGCAGGTCTCGGTTTC
GCCCTTTTGCCTCGCTATCTGATCGAGCAGGAACTTACCGCGGGCACCCTGCGGATCGTTCTCGATCGACCGATGCAGAC
GGAGAACAGCTATTATCTCGCCATTCCGGAAGGCAAGCTCGAGAATCCGATCAGTCGCGCTTTCCGGGAGTGGATCACCG
AACAGGTCGGTTGA

Upstream 100 bases:

>100_bases
GCGCTATGGATGAAGGACAAGGTCATTCTTAATTCTGATCATATCGGAAGGGTATTGGCTTGGATCGCGCTGCTTCTGAC
TATGTTGATGCGAGGCTGGA

Downstream 100 bases:

>100_bases
TCCGTAGCAGTGGTAGGTCCGGTTGAGATTGCGCATCTCGACCCTCGGATAGATGCTGCATGCACCAGGAAGGGGCGGAT
TTTGACAGTGATACCCATCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MKLSRRLVPDVTTLQAFECAARHGSFTQAAAELNLTQSAVSRQIKDLESQLGVLLFERVRQRVILSDAGQKFLPEVRRLL
NQTEELMVRAMASARADSSLSIASLPTFGSRWLVQRLPDFLKRHPGTVVNIASRSEPFDFEEQNFDLAIHYGQPVWARAI
CSYLCSEVIVPAASPALLAVHPVASPADIGGSPLLHLATRPKLWAQWFETNDVEQDGAYRGNRFDQFSMVIEAATAGLGF
ALLPRYLIEQELTAGTLRIVLDRPMQTENSYYLAIPEGKLENPISRAFREWITEQVG

Sequences:

>Translated_297_residues
MKLSRRLVPDVTTLQAFECAARHGSFTQAAAELNLTQSAVSRQIKDLESQLGVLLFERVRQRVILSDAGQKFLPEVRRLL
NQTEELMVRAMASARADSSLSIASLPTFGSRWLVQRLPDFLKRHPGTVVNIASRSEPFDFEEQNFDLAIHYGQPVWARAI
CSYLCSEVIVPAASPALLAVHPVASPADIGGSPLLHLATRPKLWAQWFETNDVEQDGAYRGNRFDQFSMVIEAATAGLGF
ALLPRYLIEQELTAGTLRIVLDRPMQTENSYYLAIPEGKLENPISRAFREWITEQVG
>Mature_297_residues
MKLSRRLVPDVTTLQAFECAARHGSFTQAAAELNLTQSAVSRQIKDLESQLGVLLFERVRQRVILSDAGQKFLPEVRRLL
NQTEELMVRAMASARADSSLSIASLPTFGSRWLVQRLPDFLKRHPGTVVNIASRSEPFDFEEQNFDLAIHYGQPVWARAI
CSYLCSEVIVPAASPALLAVHPVASPADIGGSPLLHLATRPKLWAQWFETNDVEQDGAYRGNRFDQFSMVIEAATAGLGF
ALLPRYLIEQELTAGTLRIVLDRPMQTENSYYLAIPEGKLENPISRAFREWITEQVG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=34.020618556701, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=30.4498269896194, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=27.2727272727273, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI145693193, Length=265, Percent_Identity=29.811320754717, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI145693105, Length=124, Percent_Identity=34.6774193548387, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788887, Length=120, Percent_Identity=35, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789639, Length=257, Percent_Identity=25.2918287937743, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789204, Length=214, Percent_Identity=32.2429906542056, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788481, Length=272, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=125, Percent_Identity=38.4, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787806, Length=249, Percent_Identity=26.5060240963855, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787589, Length=282, Percent_Identity=25.177304964539, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=22.3776223776224, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33165; Mature: 33165

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50931 HTH_LYSR ; PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSRRLVPDVTTLQAFECAARHGSFTQAAAELNLTQSAVSRQIKDLESQLGVLLFERVR
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRVILSDAGQKFLPEVRRLLNQTEELMVRAMASARADSSLSIASLPTFGSRWLVQRLPDF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHH
LKRHPGTVVNIASRSEPFDFEEQNFDLAIHYGQPVWARAICSYLCSEVIVPAASPALLAV
HHHCCCCEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
HPVASPADIGGSPLLHLATRPKLWAQWFETNDVEQDGAYRGNRFDQFSMVIEAATAGLGF
ECCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHH
ALLPRYLIEQELTAGTLRIVLDRPMQTENSYYLAIPEGKLENPISRAFREWITEQVG
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLSRRLVPDVTTLQAFECAARHGSFTQAAAELNLTQSAVSRQIKDLESQLGVLLFERVR
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRVILSDAGQKFLPEVRRLLNQTEELMVRAMASARADSSLSIASLPTFGSRWLVQRLPDF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHH
LKRHPGTVVNIASRSEPFDFEEQNFDLAIHYGQPVWARAICSYLCSEVIVPAASPALLAV
HHHCCCCEEEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
HPVASPADIGGSPLLHLATRPKLWAQWFETNDVEQDGAYRGNRFDQFSMVIEAATAGLGF
ECCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHH
ALLPRYLIEQELTAGTLRIVLDRPMQTENSYYLAIPEGKLENPISRAFREWITEQVG
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]