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The map label for this gene is glpK2 [H]

Identifier: 150398324

GI number: 150398324

Start: 3279052

End: 3280521

Strand: Direct

Name: glpK2 [H]

Synonym: Smed_3130

Alternate gene names: 150398324

Gene position: 3279052-3280521 (Clockwise)

Preceding gene: 150398323

Following gene: 150398325

Centisome position: 86.7

GC content: 65.58

Gene sequence:

>1470_bases
ATGACGACTGCCATTCTCGCCATCGACCAGGGCACCACGAATTCAAAAGCCGTTCTCGTTTCGACGACCGGTGAGATCCT
CTCGCGTGGCGCATCGCCGGTCGGCATCGAACATCCGCAGCCCGGCTGGGTGGAGCAGAACCCGCTTCGCATCTGGGAAT
CGGTTCAGGAGGCGATTGCCGCCTGCCTGCAGAAGGGACCCCGATCCGACATCCTCGGGATCGCGATCTCCAACCAGCGC
GAGTCGGTCACCGCCTGGGACGCCGCAACGGGCAAGCCGCTCGGCCCGGTGGTCAGCTGGCAATGCCGCCGCAGCGCGCC
GGCCTGCGCCGAACTCAGCGCCGCAGGCCATGCCGAGCGCGTCCGGGCGCTCACGGGTCTCCCCATAGACCCTATGTTCC
CCGGTCCCAAAATGAAGTGGCTGCTCGATCGCATTCCCCCGGGACATGAGGTCCGGCTGGGCACGATCGACGCGTGGCTG
ATCCATTGCTTCACGGCTGGCGCGATCCATGCCTGCGATGCCGGCAATGCCGCCCGCAGCCAGCTTTACGACCTCAACCG
CCGGGCCTGGAGCGACGAGCTTTGCGACCTGTTCGGCGTACCCAAATCCGCCCTGCCGGAGGTGTGCGACAGCGCCGCCC
GCTTCGGCGTCACGAAGAATGTGCCGGGGCTGAAGGACGGAATCCCGATCGCCTCGGCGATCGGCGACAGCCACGCCGCG
CTTTTCGGCCACGGGGCCTTCAAACCCGGCGACGGCAAAGTGACCTTCGGCACCGGCTCTTCTATCATGACGACCCTACC
CCGCTTCATCGCTCCCGAGCAGGGAATTACGACGACGATCGCCTGGTCGATCGCCGGCCGGCCGACCTATGCCTTCGAGG
GCAACATTCTCGTTTCGGCCGCGGCCCTTCCATGGATGGCCGAAATGCTCGGCCTTTCCCATGTGGAGGACCTGATCGAT
CTCGCCGCCACCGCCGAGCCCCGCGGCCCGGGTTTCGTGCCGGCCTTCGTGGGTCTCGGCGCCCCTTACTGGCACGCCGA
TGCACGAGCGCTGTTTTCCGGCATAACCTTCAACACGACGCGCGCCCAGATGGCGCGTGCGGTCACGGACTCAATGGCCT
TCCAGGTCCATGACGTCTTCAAGGCGATGTCGGCACAATCGCCGACACCGCTCGGCCGACTCTATGCGGATGGCGGGCCG
AGCAAGAACACTTTCCTGATGCAATGCGTGGCCGATACCCTGAACCATGTCATCACTCAGTGCGACGCGCCAGAGGCCTC
GGCGCTCGGCGCAGCCTATCTGGCGGGTCTCTCGCTCGGCGTTTGGCCCGATCTCGCCGCTGTCGCTTCACTGCCGCGCG
CCGGCAAACCCGTGCTGCCGCGCCCTTGCGATGCCGCAGATCGCCTCATGGTTTGGCAGGACGCAATCTACCGCTCGACC
GTTCCAGGTCCTTCAATTATGAGTGAATAA

Upstream 100 bases:

>100_bases
TCGCACGAAAGCCCTCCCGCGCATGATCATGCGCAGGTAGATTTCGTGCCGGGCGCGGCAATCCTCCTGCCGCGCCCCAT
TCCTGTCAGCCGGAGCCGCC

Downstream 100 bases:

>100_bases
AAATTCACCACTGGAGGATCCCGATGGGACGGCTCAACGAGCTTCGACTGATCGCCCGCGTAGCCCAGATGTACCATATC
GAAAGCAAGAGGCAGGCCGA

Product: carbohydrate kinase FGGY

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase 2; Glycerokinase 2; GK 2 [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTTAILAIDQGTTNSKAVLVSTTGEILSRGASPVGIEHPQPGWVEQNPLRIWESVQEAIAACLQKGPRSDILGIAISNQR
ESVTAWDAATGKPLGPVVSWQCRRSAPACAELSAAGHAERVRALTGLPIDPMFPGPKMKWLLDRIPPGHEVRLGTIDAWL
IHCFTAGAIHACDAGNAARSQLYDLNRRAWSDELCDLFGVPKSALPEVCDSAARFGVTKNVPGLKDGIPIASAIGDSHAA
LFGHGAFKPGDGKVTFGTGSSIMTTLPRFIAPEQGITTTIAWSIAGRPTYAFEGNILVSAAALPWMAEMLGLSHVEDLID
LAATAEPRGPGFVPAFVGLGAPYWHADARALFSGITFNTTRAQMARAVTDSMAFQVHDVFKAMSAQSPTPLGRLYADGGP
SKNTFLMQCVADTLNHVITQCDAPEASALGAAYLAGLSLGVWPDLAAVASLPRAGKPVLPRPCDAADRLMVWQDAIYRST
VPGPSIMSE

Sequences:

>Translated_489_residues
MTTAILAIDQGTTNSKAVLVSTTGEILSRGASPVGIEHPQPGWVEQNPLRIWESVQEAIAACLQKGPRSDILGIAISNQR
ESVTAWDAATGKPLGPVVSWQCRRSAPACAELSAAGHAERVRALTGLPIDPMFPGPKMKWLLDRIPPGHEVRLGTIDAWL
IHCFTAGAIHACDAGNAARSQLYDLNRRAWSDELCDLFGVPKSALPEVCDSAARFGVTKNVPGLKDGIPIASAIGDSHAA
LFGHGAFKPGDGKVTFGTGSSIMTTLPRFIAPEQGITTTIAWSIAGRPTYAFEGNILVSAAALPWMAEMLGLSHVEDLID
LAATAEPRGPGFVPAFVGLGAPYWHADARALFSGITFNTTRAQMARAVTDSMAFQVHDVFKAMSAQSPTPLGRLYADGGP
SKNTFLMQCVADTLNHVITQCDAPEASALGAAYLAGLSLGVWPDLAAVASLPRAGKPVLPRPCDAADRLMVWQDAIYRST
VPGPSIMSE
>Mature_488_residues
TTAILAIDQGTTNSKAVLVSTTGEILSRGASPVGIEHPQPGWVEQNPLRIWESVQEAIAACLQKGPRSDILGIAISNQRE
SVTAWDAATGKPLGPVVSWQCRRSAPACAELSAAGHAERVRALTGLPIDPMFPGPKMKWLLDRIPPGHEVRLGTIDAWLI
HCFTAGAIHACDAGNAARSQLYDLNRRAWSDELCDLFGVPKSALPEVCDSAARFGVTKNVPGLKDGIPIASAIGDSHAAL
FGHGAFKPGDGKVTFGTGSSIMTTLPRFIAPEQGITTTIAWSIAGRPTYAFEGNILVSAAALPWMAEMLGLSHVEDLIDL
AATAEPRGPGFVPAFVGLGAPYWHADARALFSGITFNTTRAQMARAVTDSMAFQVHDVFKAMSAQSPTPLGRLYADGGPS
KNTFLMQCVADTLNHVITQCDAPEASALGAAYLAGLSLGVWPDLAAVASLPRAGKPVLPRPCDAADRLMVWQDAIYRSTV
PGPSIMSE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI4504007, Length=443, Percent_Identity=35.2144469525959, Blast_Score=265, Evalue=6e-71,
Organism=Homo sapiens, GI189409120, Length=443, Percent_Identity=35.2144469525959, Blast_Score=265, Evalue=7e-71,
Organism=Homo sapiens, GI41393575, Length=501, Percent_Identity=33.1337325349301, Blast_Score=264, Evalue=2e-70,
Organism=Homo sapiens, GI42794763, Length=448, Percent_Identity=35.0446428571429, Blast_Score=264, Evalue=2e-70,
Organism=Homo sapiens, GI88196792, Length=507, Percent_Identity=27.810650887574, Blast_Score=198, Evalue=8e-51,
Organism=Escherichia coli, GI1790361, Length=493, Percent_Identity=37.3225152129817, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI1789987, Length=461, Percent_Identity=23.8611713665944, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1789168, Length=458, Percent_Identity=24.235807860262, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1790005, Length=467, Percent_Identity=25.0535331905782, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787789, Length=497, Percent_Identity=22.5352112676056, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17535599, Length=443, Percent_Identity=31.3769751693002, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI71986140, Length=475, Percent_Identity=24.8421052631579, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI71986147, Length=257, Percent_Identity=29.5719844357977, Blast_Score=111, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6321755, Length=499, Percent_Identity=28.4569138276553, Blast_Score=185, Evalue=2e-47,
Organism=Drosophila melanogaster, GI17864214, Length=501, Percent_Identity=32.9341317365269, Blast_Score=264, Evalue=9e-71,
Organism=Drosophila melanogaster, GI24654573, Length=458, Percent_Identity=32.7510917030568, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24655555, Length=454, Percent_Identity=31.4977973568282, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24655560, Length=454, Percent_Identity=31.4977973568282, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24655570, Length=454, Percent_Identity=31.4977973568282, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24655574, Length=454, Percent_Identity=31.4977973568282, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24655565, Length=454, Percent_Identity=31.4977973568282, Blast_Score=227, Evalue=1e-59,
Organism=Drosophila melanogaster, GI19922058, Length=510, Percent_Identity=26.8627450980392, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24656367, Length=503, Percent_Identity=26.8389662027833, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI21357867, Length=520, Percent_Identity=26.1538461538462, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24652871, Length=477, Percent_Identity=27.0440251572327, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24656372, Length=351, Percent_Identity=28.7749287749288, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI45552909, Length=261, Percent_Identity=27.9693486590038, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 51601; Mature: 51470

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAILAIDQGTTNSKAVLVSTTGEILSRGASPVGIEHPQPGWVEQNPLRIWESVQEAIA
CCEEEEEEECCCCCCCEEEEEECHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ACLQKGPRSDILGIAISNQRESVTAWDAATGKPLGPVVSWQCRRSAPACAELSAAGHAER
HHHHCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCHHHHCCCCCHHHHHHCCHHHH
VRALTGLPIDPMFPGPKMKWLLDRIPPGHEVRLGTIDAWLIHCFTAGAIHACDAGNAARS
HHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHCCEEECCCCCHHHH
QLYDLNRRAWSDELCDLFGVPKSALPEVCDSAARFGVTKNVPGLKDGIPIASAIGDSHAA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCE
LFGHGAFKPGDGKVTFGTGSSIMTTLPRFIAPEQGITTTIAWSIAGRPTYAFEGNILVSA
EEECCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEECCCEEEEH
AALPWMAEMLGLSHVEDLIDLAATAEPRGPGFVPAFVGLGAPYWHADARALFSGITFNTT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCCHH
RAQMARAVTDSMAFQVHDVFKAMSAQSPTPLGRLYADGGPSKNTFLMQCVADTLNHVITQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHH
CDAPEASALGAAYLAGLSLGVWPDLAAVASLPRAGKPVLPRPCDAADRLMVWQDAIYRST
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
VPGPSIMSE
CCCCHHCCC
>Mature Secondary Structure 
TTAILAIDQGTTNSKAVLVSTTGEILSRGASPVGIEHPQPGWVEQNPLRIWESVQEAIA
CEEEEEEECCCCCCCEEEEEECHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ACLQKGPRSDILGIAISNQRESVTAWDAATGKPLGPVVSWQCRRSAPACAELSAAGHAER
HHHHCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCHHHHCCCCCHHHHHHCCHHHH
VRALTGLPIDPMFPGPKMKWLLDRIPPGHEVRLGTIDAWLIHCFTAGAIHACDAGNAARS
HHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHCCEEECCCCCHHHH
QLYDLNRRAWSDELCDLFGVPKSALPEVCDSAARFGVTKNVPGLKDGIPIASAIGDSHAA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCE
LFGHGAFKPGDGKVTFGTGSSIMTTLPRFIAPEQGITTTIAWSIAGRPTYAFEGNILVSA
EEECCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCEEEECCCEEEEH
AALPWMAEMLGLSHVEDLIDLAATAEPRGPGFVPAFVGLGAPYWHADARALFSGITFNTT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCCHH
RAQMARAVTDSMAFQVHDVFKAMSAQSPTPLGRLYADGGPSKNTFLMQCVADTLNHVITQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCCCCHHHHHHHHHHHHHHHHH
CDAPEASALGAAYLAGLSLGVWPDLAAVASLPRAGKPVLPRPCDAADRLMVWQDAIYRST
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
VPGPSIMSE
CCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]