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The map label for this gene is ispA [H]

Identifier: 150398316

GI number: 150398316

Start: 3270606

End: 3271520

Strand: Direct

Name: ispA [H]

Synonym: Smed_3122

Alternate gene names: 150398316

Gene position: 3270606-3271520 (Clockwise)

Preceding gene: 150398312

Following gene: 150398317

Centisome position: 86.48

GC content: 65.57

Gene sequence:

>915_bases
ATGACAGACGAAAGATCATCCTTTCCAGAGCGCCTCAAGTCCAATGCGGTCGCGGTCGAAGCGCTGCTCGAGACATTGCT
CGACCCGGCCGTGCGTGGCGGCGAAATTTCCCGCCCTGCGAACCTGCTCGAAGCCATGCGTCATGCGGTGCTCAATGGCG
GCAAGCGGCTGCGGCCCTTTCTTGTCAGCGAAACTGCGGCGCTCCTCGGCGGCGATGTGCAAGCGGCACTGAGAATTGGC
GCTGCGCTCGAATGCATTCATTGCTACTCGCTTGTCCATGACGATCTGCCGGCGATGGACAACGACGACATGCGGCGCGG
AAAACCGACGGTGCACGTCGCCTTCGACGAGGCAACCGCGATCCTCGCGGGCGATAGCCTGCTGACCTTCTCCTTCGACA
TCATCGCGGCGCCGGAGACAGCCCTTTCCGATCGCCAGAAGACCGCGCTCGTGCTGGCGCTCGCACGGGCGGCAGGGCAC
GGGGGCATGGCTGGGGGGCAGGCACTCGACCTTGCCGCCGAAAAGAGCGCCCCCGATGAGACGGGCATCGTACTCCTGCA
ATCGATGAAGACCGGCGCGCTTCTCCGCTTCGCCTGCGAGGCGGGCGCGATCATCGCGGATGCGGCGGCCGAGGACCGCC
AGCGTCTGCGGGCCTTCGGAGAGAAGATCGGCCTCGCCTTCCAGCTTGCGGACGATCTGCTCGACCTCACCGCCGACGCC
GCGCACATAGGCAAGGCAACCGGAAAGGATGCCGCGCGAGGAAAGGGTACACTCGTCGCGCTGCACGGACAGCCCTGGGC
CGAGCAGCGGCTCGAAAGCCTGGTGGCCGAGGCGGAAGCGCTCCTTGAGCCTTATGGCGCCCGTTCGGAGGTCCTTGCCG
AGACCGCTCGTTTCATTGCCAACCGCAGGAACTGA

Upstream 100 bases:

>100_bases
AAAGGCAAGGCTCAGAAGGTGCATCATCACCGAGAGGCTGTGCGCGCTGCGACGTGAAAGCCCGTTGAAAGCCGCTCCGC
CGCATGCCAAAGAGCCTCGT

Downstream 100 bases:

>100_bases
TAGATGAGCAGATTCTGGTCGCCGATCGTTGCCGCCTTGAAGCCTTACGTTCCCGGTGAACAGCCGCGGATGGCGAACCT
CGTCAAGCTCAACACCAATG

Product: polyprenyl synthetase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTDERSSFPERLKSNAVAVEALLETLLDPAVRGGEISRPANLLEAMRHAVLNGGKRLRPFLVSETAALLGGDVQAALRIG
AALECIHCYSLVHDDLPAMDNDDMRRGKPTVHVAFDEATAILAGDSLLTFSFDIIAAPETALSDRQKTALVLALARAAGH
GGMAGGQALDLAAEKSAPDETGIVLLQSMKTGALLRFACEAGAIIADAAAEDRQRLRAFGEKIGLAFQLADDLLDLTADA
AHIGKATGKDAARGKGTLVALHGQPWAEQRLESLVAEAEALLEPYGARSEVLAETARFIANRRN

Sequences:

>Translated_304_residues
MTDERSSFPERLKSNAVAVEALLETLLDPAVRGGEISRPANLLEAMRHAVLNGGKRLRPFLVSETAALLGGDVQAALRIG
AALECIHCYSLVHDDLPAMDNDDMRRGKPTVHVAFDEATAILAGDSLLTFSFDIIAAPETALSDRQKTALVLALARAAGH
GGMAGGQALDLAAEKSAPDETGIVLLQSMKTGALLRFACEAGAIIADAAAEDRQRLRAFGEKIGLAFQLADDLLDLTADA
AHIGKATGKDAARGKGTLVALHGQPWAEQRLESLVAEAEALLEPYGARSEVLAETARFIANRRN
>Mature_303_residues
TDERSSFPERLKSNAVAVEALLETLLDPAVRGGEISRPANLLEAMRHAVLNGGKRLRPFLVSETAALLGGDVQAALRIGA
ALECIHCYSLVHDDLPAMDNDDMRRGKPTVHVAFDEATAILAGDSLLTFSFDIIAAPETALSDRQKTALVLALARAAGHG
GMAGGQALDLAAEKSAPDETGIVLLQSMKTGALLRFACEAGAIIADAAAEDRQRLRAFGEKIGLAFQLADDLLDLTADAA
HIGKATGKDAARGKGTLVALHGQPWAEQRLESLVAEAEALLEPYGARSEVLAETARFIANRRN

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=224, Percent_Identity=30.3571428571429, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1786623, Length=308, Percent_Identity=40.2597402597403, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789578, Length=245, Percent_Identity=34.6938775510204, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17505681, Length=192, Percent_Identity=27.6041666666667, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24651612, Length=213, Percent_Identity=30.0469483568075, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 32092; Mature: 31961

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDERSSFPERLKSNAVAVEALLETLLDPAVRGGEISRPANLLEAMRHAVLNGGKRLRPF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHH
LVSETAALLGGDVQAALRIGAALECIHCYSLVHDDLPAMDNDDMRRGKPTVHVAFDEATA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCEEEEEECCCEE
ILAGDSLLTFSFDIIAAPETALSDRQKTALVLALARAAGHGGMAGGQALDLAAEKSAPDE
EEECCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCC
TGIVLLQSMKTGALLRFACEAGAIIADAAAEDRQRLRAFGEKIGLAFQLADDLLDLTADA
CCEEEEECCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHIGKATGKDAARGKGTLVALHGQPWAEQRLESLVAEAEALLEPYGARSEVLAETARFIA
HHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
NRRN
CCCC
>Mature Secondary Structure 
TDERSSFPERLKSNAVAVEALLETLLDPAVRGGEISRPANLLEAMRHAVLNGGKRLRPF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHH
LVSETAALLGGDVQAALRIGAALECIHCYSLVHDDLPAMDNDDMRRGKPTVHVAFDEATA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCEEEEEECCCEE
ILAGDSLLTFSFDIIAAPETALSDRQKTALVLALARAAGHGGMAGGQALDLAAEKSAPDE
EEECCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCC
TGIVLLQSMKTGALLRFACEAGAIIADAAAEDRQRLRAFGEKIGLAFQLADDLLDLTADA
CCEEEEECCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHIGKATGKDAARGKGTLVALHGQPWAEQRLESLVAEAEALLEPYGARSEVLAETARFIA
HHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
NRRN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]