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The map label for this gene is xthA [H]

Identifier: 150398248

GI number: 150398248

Start: 3204509

End: 3205315

Strand: Direct

Name: xthA [H]

Synonym: Smed_3054

Alternate gene names: 150398248

Gene position: 3204509-3205315 (Clockwise)

Preceding gene: 150398247

Following gene: 150398251

Centisome position: 84.73

GC content: 61.21

Gene sequence:

>807_bases
ATGCCTCTTTCCATTGCCACATGGAACATCAACTCCGTTCGCCTGCGGATGCCGCTGGTCGAACATTTCCTGAAGACATG
GCAGCCGGACATCCTCTGCCTGCAGGAAATCAAATGCATCAACGACCAGTTTCCCTCCGCGCCGCTGCGCAAGCTCGGCT
ACGAATATATCGAGATGCACGGCCAGAAGGGCTATCACGGCGTCGCCACTATCTCGCGCCTGCCGCTTCACCAGCTGAGC
GATCGTCGCGATTATTGCGGTATCGGCGATGCACGGCATCTCTCGGTCGTTTTCGCAGCCGGGGGAAAGACGATCCGCCT
GCACAACTTCTATGTCCCGGCCGGCGGCGACGAGCCGGACCGCACTGTCAACCCGAAGTTCGGACACAAACTCGACTTCG
TCGATGAGATGAAGCTCCTGCACGCGGAGGCCGAAGAAGGTATCTCTTCGATCCTCGTCGGCGATCTCAACATCGCGCCG
CTCGAGCACGACGTCTGGTCGCACAAGCAGCTCCTTAAGATCGTCAGCCATACGCCGATCGAGACCGAAGGCCTTATTTC
GGTCATGACCGGCGGCGCCTGGGTCGACCTGATGCGCCGCCACACGCCACCGCCGGAAAAGCTCTACACCTGGTGGAGTT
ACAGGGCCAAGGACTGGGCCGCAGCCGACCGCGGACGCCGCCTCGACCACATCTGGTCCTCCGCCGATCTCGCAAACCAG
CTCGTCCGCGTCGAAATCATCAAGGAAGCGCGCGGCTGGGAGCGCCCATCCGACCACGTCCCGGTGATCGCCCATTTCGA
GCTTTGA

Upstream 100 bases:

>100_bases
GCGGCCTTTTTCATGCTTGTCGATCCGGGTTGAACCTGTTTTAAACGCTCCGCTTTGCCGGACGCTTCCTTTGCCGGTAA
TTTCCCCGGGAGAATGAGGA

Downstream 100 bases:

>100_bases
CGGATGGGCGCGTGCCGCCTGGCCGTGCCCCGTCAGGCAAACCGCCCGGCAAGCGCCTCGACGCGCCGGATCATCGCCTG
GATGTTTTCCCGGATGCGGG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MPLSIATWNINSVRLRMPLVEHFLKTWQPDILCLQEIKCINDQFPSAPLRKLGYEYIEMHGQKGYHGVATISRLPLHQLS
DRRDYCGIGDARHLSVVFAAGGKTIRLHNFYVPAGGDEPDRTVNPKFGHKLDFVDEMKLLHAEAEEGISSILVGDLNIAP
LEHDVWSHKQLLKIVSHTPIETEGLISVMTGGAWVDLMRRHTPPPEKLYTWWSYRAKDWAAADRGRRLDHIWSSADLANQ
LVRVEIIKEARGWERPSDHVPVIAHFEL

Sequences:

>Translated_268_residues
MPLSIATWNINSVRLRMPLVEHFLKTWQPDILCLQEIKCINDQFPSAPLRKLGYEYIEMHGQKGYHGVATISRLPLHQLS
DRRDYCGIGDARHLSVVFAAGGKTIRLHNFYVPAGGDEPDRTVNPKFGHKLDFVDEMKLLHAEAEEGISSILVGDLNIAP
LEHDVWSHKQLLKIVSHTPIETEGLISVMTGGAWVDLMRRHTPPPEKLYTWWSYRAKDWAAADRGRRLDHIWSSADLANQ
LVRVEIIKEARGWERPSDHVPVIAHFEL
>Mature_267_residues
PLSIATWNINSVRLRMPLVEHFLKTWQPDILCLQEIKCINDQFPSAPLRKLGYEYIEMHGQKGYHGVATISRLPLHQLSD
RRDYCGIGDARHLSVVFAAGGKTIRLHNFYVPAGGDEPDRTVNPKFGHKLDFVDEMKLLHAEAEEGISSILVGDLNIAPL
EHDVWSHKQLLKIVSHTPIETEGLISVMTGGAWVDLMRRHTPPPEKLYTWWSYRAKDWAAADRGRRLDHIWSSADLANQL
VRVEIIKEARGWERPSDHVPVIAHFEL

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Escherichia coli, GI1788046, Length=274, Percent_Identity=31.021897810219, Blast_Score=110, Evalue=7e-26,
Organism=Drosophila melanogaster, GI221330655, Length=278, Percent_Identity=29.4964028776978, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17136678, Length=278, Percent_Identity=29.4964028776978, Blast_Score=81, Evalue=9e-16,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30597; Mature: 30466

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSIATWNINSVRLRMPLVEHFLKTWQPDILCLQEIKCINDQFPSAPLRKLGYEYIEMH
CCCEEEEECCCCEEEECHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHCHHHHHHC
GQKGYHGVATISRLPLHQLSDRRDYCGIGDARHLSVVFAAGGKTIRLHNFYVPAGGDEPD
CCCCCCHHHHHHHCCHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCCC
RTVNPKFGHKLDFVDEMKLLHAEAEEGISSILVGDLNIAPLEHDVWSHKQLLKIVSHTPI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
ETEGLISVMTGGAWVDLMRRHTPPPEKLYTWWSYRAKDWAAADRGRRLDHIWSSADLANQ
CCCCCEEECCCCHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHH
LVRVEIIKEARGWERPSDHVPVIAHFEL
HHHHHHHHHHCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
PLSIATWNINSVRLRMPLVEHFLKTWQPDILCLQEIKCINDQFPSAPLRKLGYEYIEMH
CCEEEEECCCCEEEECHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHCHHHHHHC
GQKGYHGVATISRLPLHQLSDRRDYCGIGDARHLSVVFAAGGKTIRLHNFYVPAGGDEPD
CCCCCCHHHHHHHCCHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCCC
RTVNPKFGHKLDFVDEMKLLHAEAEEGISSILVGDLNIAPLEHDVWSHKQLLKIVSHTPI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
ETEGLISVMTGGAWVDLMRRHTPPPEKLYTWWSYRAKDWAAADRGRRLDHIWSSADLANQ
CCCCCEEECCCCHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHH
LVRVEIIKEARGWERPSDHVPVIAHFEL
HHHHHHHHHHCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]