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The map label for this gene is pdhC [H]

Identifier: 150398231

GI number: 150398231

Start: 3185190

End: 3186503

Strand: Reverse

Name: pdhC [H]

Synonym: Smed_3037

Alternate gene names: 150398231

Gene position: 3186503-3185190 (Counterclockwise)

Preceding gene: 150398232

Following gene: 150398230

Centisome position: 84.26

GC content: 63.93

Gene sequence:

>1314_bases
ATGAGCGAACGCATCCTCAAAATGCCTCGCCTCGGCGAGACGATGGAAGAAGGCAAGATTGTCGGCTGGCTGATCAAGCC
GGGCGACAGTTTCCGCCGCGGCGATCCGATCATCGAGATCGAAACCGACAAGACGATCGCCGAGTTCCCGGCGCTCGGGG
ACGGAAGGCTAGAGGAGGTTCTCGTCGAGATCGGCGACATGATCGAGGTCGGCAAGCCGCTTGCGCGCGTCGATATCGTC
TCCGGTCCCGACTGGACCGCCGAGGATGGCTCCGCGGCGGAACCGGAGACCGAAGCCGCAGTGACAAAGGCCGAGGCGAC
GGCAGATACGGCGAAAGAACCGCCTTTAGACGATAATCCCAAGCGCCCGGGTGATCGCGTCCGTGCGACGCCCCTTGCAC
GCCGATTTGCGCGCCGCAGCGGCATCGACATCAACTCCGTAGCCGGCACTGGCCGGCGCGGCCGCATCGAGAAGCATGAT
GTGCTGGCCGCCGCAGACGGTCAATCGGTGCCATCCAGGATGCCCGCTGCTGCGGCAACCAGCTACGCAGCGCTTCGGCG
CGGCCGCATGGCTTACCTCGACAGCGGCAAGGCATTCGGGGGCACAGTGCTGCTCCTTCATGGCTTTTCGGGTGACCGGA
CCACCTGGACGGCAGTCCTCGCGGGCCTCAGACGCGCCGGCAAGCGCGTCATCGCTCCGGATCTTCCCGGTCATGGACTG
ACGGAGATTGAAGCTAGCTCGCCTTCCGACCTATCCGCCGACCTTGTCGAATTTCTTGACGCGCTCGCCATCGAAAAGGT
GGACGTCGTCGCGCATTCGCTTGGGGCGGTAGCTGCCCTCGGGCTTGCCGCTTCGGAACCGCGCCGGATCGGGTCGCTTT
CGTTGATCGCCCCTGCCGGCATCGGTTCCGAAATCGACACCGGCTTCGTTCATGGCATGGCTGGTGCTCGCACGAGCGGG
GAAATAGCCCATCTCCTGCGCCGCTTGTCCGTCAACGGCATCGAGCTTTCAGAAGCTGCATTGGAAGCGATTGCGGCTGA
TCTCGCGAGAGGAAGGCTTAGGGCGCTTGCCGATGCCGTGGCGGGTCCGTCCGGCCAGAGAGTGGACAGCCTTATGGCGC
TTCAGAGGCTCGTCGCATCGATGCCGGTGCGGGTGCTCTTCGGTCTCGAAGACAGGATCATTCCGTGGCGGCAGGTCCTT
GCTTTGCCGCCGCAGGTGGCGATCCATCTCCTGTCCAGGTCCGGCCATATGCCGCAATGGGACCAGACGAAGGACGTCCT
CGAAATCCTGCTTTCCAAGGGAGAACAGCCGTGA

Upstream 100 bases:

>100_bases
TGGCCGGTGAAGACATCCCGATCTCGGTTTCGCAGGAACTCGAGTCCGGCAGCGTGCCTTCCGCAAGGATGATTGCCGAT
GTTGCCGCGAGAATGATGGC

Downstream 100 bases:

>100_bases
GCGAAGTTTCGGAAAAACTGAAGGATGCGCAACGCCGGATCGCTGCGCTGGCCAAAGCCAGCCCAGAGCTCTTCGGTGGC
TTTGCACGGGTCAGCAAGGT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSERILKMPRLGETMEEGKIVGWLIKPGDSFRRGDPIIEIETDKTIAEFPALGDGRLEEVLVEIGDMIEVGKPLARVDIV
SGPDWTAEDGSAAEPETEAAVTKAEATADTAKEPPLDDNPKRPGDRVRATPLARRFARRSGIDINSVAGTGRRGRIEKHD
VLAAADGQSVPSRMPAAAATSYAALRRGRMAYLDSGKAFGGTVLLLHGFSGDRTTWTAVLAGLRRAGKRVIAPDLPGHGL
TEIEASSPSDLSADLVEFLDALAIEKVDVVAHSLGAVAALGLAASEPRRIGSLSLIAPAGIGSEIDTGFVHGMAGARTSG
EIAHLLRRLSVNGIELSEAALEAIAADLARGRLRALADAVAGPSGQRVDSLMALQRLVASMPVRVLFGLEDRIIPWRQVL
ALPPQVAIHLLSRSGHMPQWDQTKDVLEILLSKGEQP

Sequences:

>Translated_437_residues
MSERILKMPRLGETMEEGKIVGWLIKPGDSFRRGDPIIEIETDKTIAEFPALGDGRLEEVLVEIGDMIEVGKPLARVDIV
SGPDWTAEDGSAAEPETEAAVTKAEATADTAKEPPLDDNPKRPGDRVRATPLARRFARRSGIDINSVAGTGRRGRIEKHD
VLAAADGQSVPSRMPAAAATSYAALRRGRMAYLDSGKAFGGTVLLLHGFSGDRTTWTAVLAGLRRAGKRVIAPDLPGHGL
TEIEASSPSDLSADLVEFLDALAIEKVDVVAHSLGAVAALGLAASEPRRIGSLSLIAPAGIGSEIDTGFVHGMAGARTSG
EIAHLLRRLSVNGIELSEAALEAIAADLARGRLRALADAVAGPSGQRVDSLMALQRLVASMPVRVLFGLEDRIIPWRQVL
ALPPQVAIHLLSRSGHMPQWDQTKDVLEILLSKGEQP
>Mature_436_residues
SERILKMPRLGETMEEGKIVGWLIKPGDSFRRGDPIIEIETDKTIAEFPALGDGRLEEVLVEIGDMIEVGKPLARVDIVS
GPDWTAEDGSAAEPETEAAVTKAEATADTAKEPPLDDNPKRPGDRVRATPLARRFARRSGIDINSVAGTGRRGRIEKHDV
LAAADGQSVPSRMPAAAATSYAALRRGRMAYLDSGKAFGGTVLLLHGFSGDRTTWTAVLAGLRRAGKRVIAPDLPGHGLT
EIEASSPSDLSADLVEFLDALAIEKVDVVAHSLGAVAALGLAASEPRRIGSLSLIAPAGIGSEIDTGFVHGMAGARTSGE
IAHLLRRLSVNGIELSEAALEAIAADLARGRLRALADAVAGPSGQRVDSLMALQRLVASMPVRVLFGLEDRIIPWRQVLA
LPPQVAIHLLSRSGHMPQWDQTKDVLEILLSKGEQP

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=164, Percent_Identity=29.8780487804878, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI31711992, Length=167, Percent_Identity=31.1377245508982, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1786946, Length=161, Percent_Identity=30.4347826086957, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17537937, Length=199, Percent_Identity=30.1507537688442, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17560088, Length=207, Percent_Identity=30.4347826086957, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324258, Length=174, Percent_Identity=35.0574712643678, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6321632, Length=75, Percent_Identity=41.3333333333333, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46351; Mature: 46219

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERILKMPRLGETMEEGKIVGWLIKPGDSFRRGDPIIEIETDKTIAEFPALGDGRLEEV
CCCCHHCCCCCCCHHHCCCEEEEEECCCCCCCCCCCEEEEECCCHHHHCCCCCCCHHHHH
LVEIGDMIEVGKPLARVDIVSGPDWTAEDGSAAEPETEAAVTKAEATADTAKEPPLDDNP
HHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
KRPGDRVRATPLARRFARRSGIDINSVAGTGRRGRIEKHDVLAAADGQSVPSRMPAAAAT
CCCCCCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCHHHEEEECCCCCCCCCCCHHHHH
SYAALRRGRMAYLDSGKAFGGTVLLLHGFSGDRTTWTAVLAGLRRAGKRVIAPDLPGHGL
HHHHHHCCCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCC
TEIEASSPSDLSADLVEFLDALAIEKVDVVAHSLGAVAALGLAASEPRRIGSLSLIAPAG
CEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
IGSEIDTGFVHGMAGARTSGEIAHLLRRLSVNGIELSEAALEAIAADLARGRLRALADAV
CCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGPSGQRVDSLMALQRLVASMPVRVLFGLEDRIIPWRQVLALPPQVAIHLLSRSGHMPQW
CCCCCHHHHHHHHHHHHHHHCCHHEEECCCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCC
DQTKDVLEILLSKGEQP
CCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SERILKMPRLGETMEEGKIVGWLIKPGDSFRRGDPIIEIETDKTIAEFPALGDGRLEEV
CCCHHCCCCCCCHHHCCCEEEEEECCCCCCCCCCCEEEEECCCHHHHCCCCCCCHHHHH
LVEIGDMIEVGKPLARVDIVSGPDWTAEDGSAAEPETEAAVTKAEATADTAKEPPLDDNP
HHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
KRPGDRVRATPLARRFARRSGIDINSVAGTGRRGRIEKHDVLAAADGQSVPSRMPAAAAT
CCCCCCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCHHHEEEECCCCCCCCCCCHHHHH
SYAALRRGRMAYLDSGKAFGGTVLLLHGFSGDRTTWTAVLAGLRRAGKRVIAPDLPGHGL
HHHHHHCCCEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCC
TEIEASSPSDLSADLVEFLDALAIEKVDVVAHSLGAVAALGLAASEPRRIGSLSLIAPAG
CEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
IGSEIDTGFVHGMAGARTSGEIAHLLRRLSVNGIELSEAALEAIAADLARGRLRALADAV
CCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGPSGQRVDSLMALQRLVASMPVRVLFGLEDRIIPWRQVLALPPQVAIHLLSRSGHMPQW
CCCCCHHHHHHHHHHHHHHHCCHHEEECCCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCC
DQTKDVLEILLSKGEQP
CCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA