Click here to switch to the map view.

The map label for this gene is mcl2 [H]

Identifier: 150398221

GI number: 150398221

Start: 3175836

End: 3176741

Strand: Reverse

Name: mcl2 [H]

Synonym: Smed_3026

Alternate gene names: 150398221

Gene position: 3176741-3175836 (Counterclockwise)

Preceding gene: 150398227

Following gene: 150398220

Centisome position: 84.0

GC content: 64.24

Gene sequence:

>906_bases
ATGACGAGAGCGATCGACCATCACCCCGTGCGGCTGCGCCGTTCGGTGCTCTCGGTTCCGGCCGACAATCAGCGCGCGCT
CGCCAAGGTCCGCGATCTGGCGGCGGACGCCGTGATCTTCGACCTCGAGGACGCCGTCTCGCCGGACCGGAAGGGCGAGG
CACGCGATGCGCTCGTCCGTCATCTTGCCGGCAACCGGCCGCAGGCCGAAACGATCATCCGCGTCAATGCGGCGGGTTCG
GACTTCGGCGAGGCCGATCTCGCAGCGGCGCTTGCCGCGCGGCCCGATGCTATCCTCCTGCCAAAGGTCGAAAGCCCTGC
CGATATCCAGCGCGTTCTCGACTGGCTTTCGGAAAGGGATGCCCCGGAAGAACTGAGGCTCTGGGCGATGATCGAGACGC
CGCGCGGGGTCGTAAACGGACCGGCCATCGCCGAGACCGGCCGCACCTTCGGCGGACGGCTGGATTGCTTCGTCGTCGGG
CTGAACGATCTCCGCAAGGAGACGGGCGTACCCGCCCTTCCCGGCCGTAACTATCTCGTCCCCTGGCTGATGCAGATTCT
TCTTGCCGCGCGCGGCAGCGGGCTCGACGTGATCGATGCCGTCTTCAATGATTTCCGCGATCCGGAAGGCTTTGACACGG
AGTGCCGGCAGGGACGCGATATGGGCTATGACGGCAAGATGCTGATTCATCCGGCCCAGATCGATGCGGCCAATCGGTAT
TTCGGCCTGGAAGAGGCGGCGATCGAGGAGGCGCTTTCGATCATCGAGGCCTTCAGCCTGCCGGAAAATGCCGGAAAGGG
CGTCATCAATCTTCATGGCCGCATGGTCGAGCGCCTCCACCTCGAGCAGGCGATGAAAATCGCCGCCAAGGCGGAGATCA
TCGAAAAGCGAAAGGCAAGATCTTGA

Upstream 100 bases:

>100_bases
GCTTGCAAGAGGGTCCGCCTATCGCGTCTTGCGCATAGCTTGTCTCGTCTTGCGCAAAGGTTGAAAGGCAGGCACAACGA
ATGCCATGACAGGGAACGGC

Downstream 100 bases:

>100_bases
AACTCTACCGCTTTCTAACCGGCCCCGACGATGCCTCCTTCTGTCACAAGGTGACGGCGGCATTGAACCAGGGGTGGACG
CTGCATGGCCCGACGACCTA

Product: citrate (pro-3S)-lyase

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTRAIDHHPVRLRRSVLSVPADNQRALAKVRDLAADAVIFDLEDAVSPDRKGEARDALVRHLAGNRPQAETIIRVNAAGS
DFGEADLAAALAARPDAILLPKVESPADIQRVLDWLSERDAPEELRLWAMIETPRGVVNGPAIAETGRTFGGRLDCFVVG
LNDLRKETGVPALPGRNYLVPWLMQILLAARGSGLDVIDAVFNDFRDPEGFDTECRQGRDMGYDGKMLIHPAQIDAANRY
FGLEEAAIEEALSIIEAFSLPENAGKGVINLHGRMVERLHLEQAMKIAAKAEIIEKRKARS

Sequences:

>Translated_301_residues
MTRAIDHHPVRLRRSVLSVPADNQRALAKVRDLAADAVIFDLEDAVSPDRKGEARDALVRHLAGNRPQAETIIRVNAAGS
DFGEADLAAALAARPDAILLPKVESPADIQRVLDWLSERDAPEELRLWAMIETPRGVVNGPAIAETGRTFGGRLDCFVVG
LNDLRKETGVPALPGRNYLVPWLMQILLAARGSGLDVIDAVFNDFRDPEGFDTECRQGRDMGYDGKMLIHPAQIDAANRY
FGLEEAAIEEALSIIEAFSLPENAGKGVINLHGRMVERLHLEQAMKIAAKAEIIEKRKARS
>Mature_300_residues
TRAIDHHPVRLRRSVLSVPADNQRALAKVRDLAADAVIFDLEDAVSPDRKGEARDALVRHLAGNRPQAETIIRVNAAGSD
FGEADLAAALAARPDAILLPKVESPADIQRVLDWLSERDAPEELRLWAMIETPRGVVNGPAIAETGRTFGGRLDCFVVGL
NDLRKETGVPALPGRNYLVPWLMQILLAARGSGLDVIDAVFNDFRDPEGFDTECRQGRDMGYDGKMLIHPAQIDAANRYF
GLEEAAIEEALSIIEAFSLPENAGKGVINLHGRMVERLHLEQAMKIAAKAEIIEKRKARS

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=303, Percent_Identity=32.6732673267327, Blast_Score=151, Evalue=6e-37,
Organism=Escherichia coli, GI87081764, Length=302, Percent_Identity=30.4635761589404, Blast_Score=113, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17557458, Length=309, Percent_Identity=29.7734627831715, Blast_Score=114, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6; 4.1.3.34

Molecular weight: Translated: 33004; Mature: 32873

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRAIDHHPVRLRRSVLSVPADNQRALAKVRDLAADAVIFDLEDAVSPDRKGEARDALVR
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECHHHCCCCCCCHHHHHHHH
HLAGNRPQAETIIRVNAAGSDFGEADLAAALAARPDAILLPKVESPADIQRVLDWLSERD
HHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCC
APEELRLWAMIETPRGVVNGPAIAETGRTFGGRLDCFVVGLNDLRKETGVPALPGRNYLV
CHHHHEEEEEEECCCCCCCCCCHHHCCCCCCCCEEEEEECHHHHHHHCCCCCCCCCCCHH
PWLMQILLAARGSGLDVIDAVFNDFRDPEGFDTECRQGRDMGYDGKMLIHPAQIDAANRY
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCEEEECCHHCCCHHH
FGLEEAAIEEALSIIEAFSLPENAGKGVINLHGRMVERLHLEQAMKIAAKAEIIEKRKAR
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
S
C
>Mature Secondary Structure 
TRAIDHHPVRLRRSVLSVPADNQRALAKVRDLAADAVIFDLEDAVSPDRKGEARDALVR
CCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEECHHHCCCCCCCHHHHHHHH
HLAGNRPQAETIIRVNAAGSDFGEADLAAALAARPDAILLPKVESPADIQRVLDWLSERD
HHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCC
APEELRLWAMIETPRGVVNGPAIAETGRTFGGRLDCFVVGLNDLRKETGVPALPGRNYLV
CHHHHEEEEEEECCCCCCCCCCHHHCCCCCCCCEEEEEECHHHHHHHCCCCCCCCCCCHH
PWLMQILLAARGSGLDVIDAVFNDFRDPEGFDTECRQGRDMGYDGKMLIHPAQIDAANRY
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCEEEECCHHCCCHHH
FGLEEAAIEEALSIIEAFSLPENAGKGVINLHGRMVERLHLEQAMKIAAKAEIIEKRKAR
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA