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The map label for this gene is lutR [H]

Identifier: 150398175

GI number: 150398175

Start: 3126291

End: 3126980

Strand: Reverse

Name: lutR [H]

Synonym: Smed_2978

Alternate gene names: 150398175

Gene position: 3126980-3126291 (Counterclockwise)

Preceding gene: 150398182

Following gene: 150398174

Centisome position: 82.68

GC content: 60.58

Gene sequence:

>690_bases
ATGCTCAGCAAGACACTCGTTCCGCAATCGGTCGCACGCGAAATTCAGACCATGATCAACAGCGGAACATTGAAGGCGGG
GGAAAAGATTCCCTCCCAGCGGGAGTTCTCGCAGAAGTTCGGCATCAGCCGCGCTTCTCTGCGGGAAGCCCTTCTGACCC
TGGAAACGCTTGGTCTGCTGAAGACGGAGCCGGGCCGTGGCACTTTCGTCGCCGAGGCGCAGGGCCGGCCCTCCGGTGAC
ATGGCGCCGTGGCGTTACTCCGACAGCTATTCCGTATTCGATGTATTTCAGACCCGGATCATGCTTGAAGGCGAAATCGC
ACGGCTTTCCGCCGGACGCCTCACACAAATCCAGCTCGAAAGGATGGAAAGGGCGACCCGTACGATGGAGGAGTGCTGGG
CCAATCAGGACCTCCTGGCGAATGTGGAGGCGGACCTGGAGTTCCATGGGGTCATCGTTTCCGCCTGCTCGAACGCGATG
CTGCGCGCGCTCTATCAGACCGTACGTGACCAGCTTACCGAGACCCAACGTCAACCAATCCCTCACACGGATCCAGAGCG
GATGAAGGCTTCGATTTCAGAGCACCGGCGTATCATCGCCGCGCTTGCGGCCAATGACGGGCTTGGCGCCAGGCATGAAA
TGGAGACGCATATCCGCAACACCGCGCAGTGTGCGGGGCTCGCGCCCTGA

Upstream 100 bases:

>100_bases
CAGTTAGACCAGACAATCACGGGTCAAGGCGCTTTGCAAGGTCGACGATAAAAAATAGATTGGGCCGATATGCCCGGGTC
CGGGAAGAAGAAGGGTTCGC

Downstream 100 bases:

>100_bases
CCCGGTGCCGGGCAACGCCGTGAACCTCCCTTCACGGAACTGTCGCCAAACCGTTGAAATAGTTTCATCCGTCTGTAACG
TCACTGACATCCGGCTCGCC

Product: GntR domain-containing protein

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MLSKTLVPQSVAREIQTMINSGTLKAGEKIPSQREFSQKFGISRASLREALLTLETLGLLKTEPGRGTFVAEAQGRPSGD
MAPWRYSDSYSVFDVFQTRIMLEGEIARLSAGRLTQIQLERMERATRTMEECWANQDLLANVEADLEFHGVIVSACSNAM
LRALYQTVRDQLTETQRQPIPHTDPERMKASISEHRRIIAALAANDGLGARHEMETHIRNTAQCAGLAP

Sequences:

>Translated_229_residues
MLSKTLVPQSVAREIQTMINSGTLKAGEKIPSQREFSQKFGISRASLREALLTLETLGLLKTEPGRGTFVAEAQGRPSGD
MAPWRYSDSYSVFDVFQTRIMLEGEIARLSAGRLTQIQLERMERATRTMEECWANQDLLANVEADLEFHGVIVSACSNAM
LRALYQTVRDQLTETQRQPIPHTDPERMKASISEHRRIIAALAANDGLGARHEMETHIRNTAQCAGLAP
>Mature_229_residues
MLSKTLVPQSVAREIQTMINSGTLKAGEKIPSQREFSQKFGISRASLREALLTLETLGLLKTEPGRGTFVAEAQGRPSGD
MAPWRYSDSYSVFDVFQTRIMLEGEIARLSAGRLTQIQLERMERATRTMEECWANQDLLANVEADLEFHGVIVSACSNAM
LRALYQTVRDQLTETQRQPIPHTDPERMKASISEHRRIIAALAANDGLGARHEMETHIRNTAQCAGLAP

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=221, Percent_Identity=27.6018099547511, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1786303, Length=211, Percent_Identity=32.7014218009479, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789353, Length=204, Percent_Identity=28.921568627451, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87082208, Length=223, Percent_Identity=26.457399103139, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1790032, Length=217, Percent_Identity=27.1889400921659, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789621, Length=229, Percent_Identity=24.4541484716157, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI48994955, Length=208, Percent_Identity=26.9230769230769, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 25497; Mature: 25497

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKTLVPQSVAREIQTMINSGTLKAGEKIPSQREFSQKFGISRASLREALLTLETLGLL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KTEPGRGTFVAEAQGRPSGDMAPWRYSDSYSVFDVFQTRIMLEGEIARLSAGRLTQIQLE
CCCCCCCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHH
RMERATRTMEECWANQDLLANVEADLEFHGVIVSACSNAMLRALYQTVRDQLTETQRQPI
HHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PHTDPERMKASISEHRRIIAALAANDGLGARHEMETHIRNTAQCAGLAP
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLSKTLVPQSVAREIQTMINSGTLKAGEKIPSQREFSQKFGISRASLREALLTLETLGLL
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KTEPGRGTFVAEAQGRPSGDMAPWRYSDSYSVFDVFQTRIMLEGEIARLSAGRLTQIQLE
CCCCCCCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHH
RMERATRTMEECWANQDLLANVEADLEFHGVIVSACSNAMLRALYQTVRDQLTETQRQPI
HHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PHTDPERMKASISEHRRIIAALAANDGLGARHEMETHIRNTAQCAGLAP
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]