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The map label for this gene is mdh [H]

Identifier: 150398141

GI number: 150398141

Start: 3092513

End: 3093475

Strand: Reverse

Name: mdh [H]

Synonym: Smed_2944

Alternate gene names: 150398141

Gene position: 3093475-3092513 (Counterclockwise)

Preceding gene: 150398142

Following gene: 150398140

Centisome position: 81.8

GC content: 62.62

Gene sequence:

>963_bases
ATGGCGCGCAACAAGATCGCACTTATCGGCTCGGGAATGATTGGTGGCACGCTGGCGCATCTCGCCGGCCTGAAGGAACT
GGGCGACATCGTCCTCTTTGACATTGCCGACGGCATTCCCCAGGGCAAGGGGCTCGACATCGCTCAGTCCTCTCCGGTCG
AGGGCTTCGATGCCTCGCTCACGGGTGCGAGCGACTATTCTGCGATCGAAGGCGCCGACGTCTGCATCGTCACGGCGGGC
GTGCCGCGCAAGCCGGGCATGAGCCGCGACGATCTGCTCGGCATCAACCTCAAGGTCATGGAGCAGGTCGGCGCCGGCAT
CAAGAAATATGCTCCGAACGCCTTCGTCATCTGCATCACCAACCCGCTCGACGCGATGGTCTGGGCGTTGCAGAAATTCT
CCGGGCTGCCGAAGAACAAGGTCGTCGGCATGGCCGGCGTGCTCGATTCCTCGCGCTTCCGGCTCTTCCTTGCCGAGGAG
TTCAACGTCTCGGTCAAGGACATCACCGCTTTCGTGCTCGGCGGCCATGGCGATACCATGGTGCCGCTCGCCCGTTATTC
GACCGTTGCCGGCATTCCGCTTCCCGATCTCATCCAGATGGGCTGGACCACCAAGGAGAAGCTCGACCAGATCATCCAGC
GCACCCGCGACGGCGGCGCCGAGATCGTCGGCCTGCTCAAGACCGGCTCGGCCTATTATGCCCCGGCCGCGTCCGCGATC
GAAATGGCCGAAGCCTATCTCAAGGACAAGAAACGCGTGCTGCCCTGCGCGGCGCATCTTTCCGGACAGTATGGCGTCAA
GGAGATGTATGTCGGCGTACCGACCGTGATCGGCGCCGGCGGCGTCGAACGCATTATCGAAATCGACCTCAACAAGGGCG
AGAAGGAAGCCTTCGACAAGTCGGTGGCGGCCGTGGCCGGCCTTTGCGAAGCCTGCATCAACATCGCACCGAGCCTGAAG
TAA

Upstream 100 bases:

>100_bases
TGATATTTTTCCCTTTGCCGTTTAAAGGGCTTTCGCGAAGGTTTGGCGTTTGCCGCGTTTCAGGCCTTGATCATTTTGGG
ATCACAAAGGAAGCACTTTC

Downstream 100 bases:

>100_bases
TCGCCAACCGGCCCATCAGACAGGAAAGAACCCATGAACATCCATGAATATCAGGCCAAGGCTCTCTTGAAGAGCTACGG
CGCGCCGGTCGCGGAAGGCG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MARNKIALIGSGMIGGTLAHLAGLKELGDIVLFDIADGIPQGKGLDIAQSSPVEGFDASLTGASDYSAIEGADVCIVTAG
VPRKPGMSRDDLLGINLKVMEQVGAGIKKYAPNAFVICITNPLDAMVWALQKFSGLPKNKVVGMAGVLDSSRFRLFLAEE
FNVSVKDITAFVLGGHGDTMVPLARYSTVAGIPLPDLIQMGWTTKEKLDQIIQRTRDGGAEIVGLLKTGSAYYAPAASAI
EMAEAYLKDKKRVLPCAAHLSGQYGVKEMYVGVPTVIGAGGVERIIEIDLNKGEKEAFDKSVAAVAGLCEACINIAPSLK

Sequences:

>Translated_320_residues
MARNKIALIGSGMIGGTLAHLAGLKELGDIVLFDIADGIPQGKGLDIAQSSPVEGFDASLTGASDYSAIEGADVCIVTAG
VPRKPGMSRDDLLGINLKVMEQVGAGIKKYAPNAFVICITNPLDAMVWALQKFSGLPKNKVVGMAGVLDSSRFRLFLAEE
FNVSVKDITAFVLGGHGDTMVPLARYSTVAGIPLPDLIQMGWTTKEKLDQIIQRTRDGGAEIVGLLKTGSAYYAPAASAI
EMAEAYLKDKKRVLPCAAHLSGQYGVKEMYVGVPTVIGAGGVERIIEIDLNKGEKEAFDKSVAAVAGLCEACINIAPSLK
>Mature_319_residues
ARNKIALIGSGMIGGTLAHLAGLKELGDIVLFDIADGIPQGKGLDIAQSSPVEGFDASLTGASDYSAIEGADVCIVTAGV
PRKPGMSRDDLLGINLKVMEQVGAGIKKYAPNAFVICITNPLDAMVWALQKFSGLPKNKVVGMAGVLDSSRFRLFLAEEF
NVSVKDITAFVLGGHGDTMVPLARYSTVAGIPLPDLIQMGWTTKEKLDQIIQRTRDGGAEIVGLLKTGSAYYAPAASAIE
MAEAYLKDKKRVLPCAAHLSGQYGVKEMYVGVPTVIGAGGVERIIEIDLNKGEKEAFDKSVAAVAGLCEACINIAPSLK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=305, Percent_Identity=30.4918032786885, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI221136809, Length=305, Percent_Identity=30.4918032786885, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=31.8032786885246, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=31.8032786885246, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI5031857, Length=312, Percent_Identity=29.1666666666667, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI260099723, Length=312, Percent_Identity=29.1666666666667, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI15082234, Length=303, Percent_Identity=27.3927392739274, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI291575128, Length=303, Percent_Identity=29.3729372937294, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI4557032, Length=303, Percent_Identity=29.3729372937294, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI260099727, Length=221, Percent_Identity=31.6742081447964, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI260099725, Length=200, Percent_Identity=32.5, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI207028494, Length=195, Percent_Identity=29.7435897435897, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI21735621, Length=310, Percent_Identity=27.4193548387097, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI103472011, Length=311, Percent_Identity=20.5787781350482, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1789632, Length=297, Percent_Identity=30.976430976431, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17535107, Length=284, Percent_Identity=26.056338028169, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17554310, Length=315, Percent_Identity=30.4761904761905, Blast_Score=97, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6322765, Length=299, Percent_Identity=28.4280936454849, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320125, Length=255, Percent_Identity=28.2352941176471, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324446, Length=255, Percent_Identity=27.843137254902, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17136226, Length=302, Percent_Identity=28.476821192053, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI45550422, Length=302, Percent_Identity=25.8278145695364, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24647881, Length=245, Percent_Identity=30.6122448979592, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24663595, Length=289, Percent_Identity=28.3737024221453, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24663599, Length=314, Percent_Identity=27.0700636942675, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 33612; Mature: 33480

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNKIALIGSGMIGGTLAHLAGLKELGDIVLFDIADGIPQGKGLDIAQSSPVEGFDASL
CCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
TGASDYSAIEGADVCIVTAGVPRKPGMSRDDLLGINLKVMEQVGAGIKKYAPNAFVICIT
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKFSGLPKNKVVGMAGVLDSSRFRLFLAEEFNVSVKDITAFVLGGHGDTM
CHHHHHHHHHHHHCCCCCHHEEEEHHEECCCCEEEEEECCCCCCHHHEEEEEECCCCCCC
VPLARYSTVAGIPLPDLIQMGWTTKEKLDQIIQRTRDGGAEIVGLLKTGSAYYAPAASAI
CCHHHHHEECCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHH
EMAEAYLKDKKRVLPCAAHLSGQYGVKEMYVGVPTVIGAGGVERIIEIDLNKGEKEAFDK
HHHHHHHHHCHHHCHHHHHCCCCCCHHHHHCCCCHHCCCCCCEEEEEEECCCCHHHHHHH
SVAAVAGLCEACINIAPSLK
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ARNKIALIGSGMIGGTLAHLAGLKELGDIVLFDIADGIPQGKGLDIAQSSPVEGFDASL
CCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
TGASDYSAIEGADVCIVTAGVPRKPGMSRDDLLGINLKVMEQVGAGIKKYAPNAFVICIT
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHCCCHHHHCCCEEEEEEC
NPLDAMVWALQKFSGLPKNKVVGMAGVLDSSRFRLFLAEEFNVSVKDITAFVLGGHGDTM
CHHHHHHHHHHHHCCCCCHHEEEEHHEECCCCEEEEEECCCCCCHHHEEEEEECCCCCCC
VPLARYSTVAGIPLPDLIQMGWTTKEKLDQIIQRTRDGGAEIVGLLKTGSAYYAPAASAI
CCHHHHHEECCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHH
EMAEAYLKDKKRVLPCAAHLSGQYGVKEMYVGVPTVIGAGGVERIIEIDLNKGEKEAFDK
HHHHHHHHHCHHHCHHHHHCCCCCCHHHHHCCCCHHCCCCCCEEEEEEECCCCHHHHHHH
SVAAVAGLCEACINIAPSLK
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA