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The map label for this gene is bepF [H]

Identifier: 150397842

GI number: 150397842

Start: 2747426

End: 2748583

Strand: Reverse

Name: bepF [H]

Synonym: Smed_2644

Alternate gene names: 150397842

Gene position: 2748583-2747426 (Counterclockwise)

Preceding gene: 150397843

Following gene: 150397841

Centisome position: 72.68

GC content: 64.94

Gene sequence:

>1158_bases
ATGTTGCGAAAGTGGCGTTTGCCGGCCGTCGCCGGCGTTTTCGTCTCCCTTCTCGTCTCGATCCCGGCGGTGGCGCAGGA
CGCGCAGATGCCGACCGTGACCGTTGCCAAACCAGTCGTGCGCGACGTCGTCGACGCCGACGAATTCATCGGCCGTTTCG
AAGCGGTCGATGAGGTTTCGATCCGCGCGCGGGTCGGAGGCTATCTCGACCAGGTCTTCTTCACCGATGGTGCGCTGGTG
AAGAAGGGCGACAAGCTCTTCGTCATCGACCAGCGGCCGTTTCGAACCGCTCTGTCCCAGGCGGAAGCCTCGCTCGAGGC
GGCGCGCTCGACGCTCGTGTTTGCAGAGACGACGTTCAAACGGACGGAGTCCCTGGCCGGGTCTGGCACCCTGTCCGTGT
CACGGCTGGACGATGACCGCCGCGCGCTGATTGCGGCGCAGGCGAATGTGCGCGGAGCCGAGGCGGCCGTCGAGCGGGCC
AAGCTCGACCTAGACTACACGACCATCACAGCCCCTCTGAGCGGCCGCGTCGATCGGAGGCTCATCTCCACCGGAAATCT
CGTCCAGGCGGACCAGACGATACTGACGACGATCGTCTCTCTTGACCCGATCGATTTTTATTTCGACGTCGATGAGCGCC
GGCTGCTTTCCTACGCCCGTACCGCCCGGGATCGCGGCAGCGCGCTGCAGGAAGGGGCAGGCTCGATTCCGGTGCTGGTG
ACGATCGCCGACGCCAACGAGCAGCCATTCGAAGGCAAGCTCGATTTTGCGGAGAACCGCGTCGACAGCGAAACCGGCAC
GATGCGGGTGCGCGCCCGCTTCCCGAATCCAAAATTCGTCCTGCAGCCCGGCCTGTTCGGGCGTGTGGAAGTCGAAGGCT
CGAACACCTATCGGGCAGTCCTCGTGCCTGACCAGGCAATTGCCGCCGACCAGAACGAACGCGTCGTCTACGTCGTGGGC
GAAGACGGCAGCGTCTCGACCAGGCCGGTGCGCCTCGGGCCGAGGCTTCACGGTTATCGGGTGATCCGCAGCGGCATGAC
AGGAGACGAGACGATCGTCGTCAACGGCCTCATGCGCGTGCGGCCGGGCGTGAAGGTGAAGCCCGAAATCGTCGTCCTGC
CCCCAGAGGCTGCGCAATCGGCGGAGAACGCCCAATGA

Upstream 100 bases:

>100_bases
GCAACAGAAACGCTGGAGGGGTCTCGGTATCGAATTGTCGCCGCGCCAATTGCAAGCCCCGGTGCCGGCGATCCGATGTT
CAGATATCTGGAGAGCGTGC

Downstream 100 bases:

>100_bases
GGTTCGCTCATTTCTTTGTCGACAGACCGATCTTTGCGTCGGTGCTCTCGATCGTCCTGTTGATCGTCGGCAGCATCGCC
TATTTCCAGCTTCCGATCGC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MLRKWRLPAVAGVFVSLLVSIPAVAQDAQMPTVTVAKPVVRDVVDADEFIGRFEAVDEVSIRARVGGYLDQVFFTDGALV
KKGDKLFVIDQRPFRTALSQAEASLEAARSTLVFAETTFKRTESLAGSGTLSVSRLDDDRRALIAAQANVRGAEAAVERA
KLDLDYTTITAPLSGRVDRRLISTGNLVQADQTILTTIVSLDPIDFYFDVDERRLLSYARTARDRGSALQEGAGSIPVLV
TIADANEQPFEGKLDFAENRVDSETGTMRVRARFPNPKFVLQPGLFGRVEVEGSNTYRAVLVPDQAIAADQNERVVYVVG
EDGSVSTRPVRLGPRLHGYRVIRSGMTGDETIVVNGLMRVRPGVKVKPEIVVLPPEAAQSAENAQ

Sequences:

>Translated_385_residues
MLRKWRLPAVAGVFVSLLVSIPAVAQDAQMPTVTVAKPVVRDVVDADEFIGRFEAVDEVSIRARVGGYLDQVFFTDGALV
KKGDKLFVIDQRPFRTALSQAEASLEAARSTLVFAETTFKRTESLAGSGTLSVSRLDDDRRALIAAQANVRGAEAAVERA
KLDLDYTTITAPLSGRVDRRLISTGNLVQADQTILTTIVSLDPIDFYFDVDERRLLSYARTARDRGSALQEGAGSIPVLV
TIADANEQPFEGKLDFAENRVDSETGTMRVRARFPNPKFVLQPGLFGRVEVEGSNTYRAVLVPDQAIAADQNERVVYVVG
EDGSVSTRPVRLGPRLHGYRVIRSGMTGDETIVVNGLMRVRPGVKVKPEIVVLPPEAAQSAENAQ
>Mature_385_residues
MLRKWRLPAVAGVFVSLLVSIPAVAQDAQMPTVTVAKPVVRDVVDADEFIGRFEAVDEVSIRARVGGYLDQVFFTDGALV
KKGDKLFVIDQRPFRTALSQAEASLEAARSTLVFAETTFKRTESLAGSGTLSVSRLDDDRRALIAAQANVRGAEAAVERA
KLDLDYTTITAPLSGRVDRRLISTGNLVQADQTILTTIVSLDPIDFYFDVDERRLLSYARTARDRGSALQEGAGSIPVLV
TIADANEQPFEGKLDFAENRVDSETGTMRVRARFPNPKFVLQPGLFGRVEVEGSNTYRAVLVPDQAIAADQNERVVYVVG
EDGSVSTRPVRLGPRLHGYRVIRSGMTGDETIVVNGLMRVRPGVKVKPEIVVLPPEAAQSAENAQ

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=323, Percent_Identity=31.8885448916409, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1786668, Length=349, Percent_Identity=32.6647564469914, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI87082036, Length=342, Percent_Identity=30.4093567251462, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789665, Length=327, Percent_Identity=31.8042813455657, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI87081951, Length=164, Percent_Identity=31.7073170731707, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41912; Mature: 41912

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKWRLPAVAGVFVSLLVSIPAVAQDAQMPTVTVAKPVVRDVVDADEFIGRFEAVDEVS
CCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCEEEHHHHHHHHHHCHHHHHHHHHHHHHHE
IRARVGGYLDQVFFTDGALVKKGDKLFVIDQRPFRTALSQAEASLEAARSTLVFAETTFK
EHHHHCHHHHHHHCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEHHHH
RTESLAGSGTLSVSRLDDDRRALIAAQANVRGAEAAVERAKLDLDYTTITAPLSGRVDRR
HHHHCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHEECCEEEEECCCCCCCHHH
LISTGNLVQADQTILTTIVSLDPIDFYFDVDERRLLSYARTARDRGSALQEGAGSIPVLV
EECCCCEEECCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCHHHCCCCCCEEEE
TIADANEQPFEGKLDFAENRVDSETGTMRVRARFPNPKFVLQPGLFGRVEVEGSNTYRAV
EEECCCCCCCCCCCCHHHCCCCCCCCEEEEEEECCCCCEEECCCCEEEEEECCCCEEEEE
LVPDQAIAADQNERVVYVVGEDGSVSTRPVRLGPRLHGYRVIRSGMTGDETIVVNGLMRV
EECCHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCHHEECCCCCCCEEEEECCEEC
RPGVKVKPEIVVLPPEAAQSAENAQ
CCCCEECCEEEEECCHHHCCCCCCC
>Mature Secondary Structure
MLRKWRLPAVAGVFVSLLVSIPAVAQDAQMPTVTVAKPVVRDVVDADEFIGRFEAVDEVS
CCCCCCCHHHHHHHHHHHHHCCHHHCCCCCCEEEHHHHHHHHHHCHHHHHHHHHHHHHHE
IRARVGGYLDQVFFTDGALVKKGDKLFVIDQRPFRTALSQAEASLEAARSTLVFAETTFK
EHHHHCHHHHHHHCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEHHHH
RTESLAGSGTLSVSRLDDDRRALIAAQANVRGAEAAVERAKLDLDYTTITAPLSGRVDRR
HHHHCCCCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHEECCEEEEECCCCCCCHHH
LISTGNLVQADQTILTTIVSLDPIDFYFDVDERRLLSYARTARDRGSALQEGAGSIPVLV
EECCCCEEECCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCHHHCCCCCCEEEE
TIADANEQPFEGKLDFAENRVDSETGTMRVRARFPNPKFVLQPGLFGRVEVEGSNTYRAV
EEECCCCCCCCCCCCHHHCCCCCCCCEEEEEEECCCCCEEECCCCEEEEEECCCCEEEEE
LVPDQAIAADQNERVVYVVGEDGSVSTRPVRLGPRLHGYRVIRSGMTGDETIVVNGLMRV
EECCHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCHHEECCCCCCCEEEEECCEEC
RPGVKVKPEIVVLPPEAAQSAENAQ
CCCCEECCEEEEECCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]