Click here to switch to the map view.

The map label for this gene is galE [C]

Identifier: 150397831

GI number: 150397831

Start: 2734869

End: 2736089

Strand: Direct

Name: galE [C]

Synonym: Smed_2633

Alternate gene names: 150397831

Gene position: 2734869-2736089 (Clockwise)

Preceding gene: 150397829

Following gene: 150397832

Centisome position: 72.31

GC content: 59.3

Gene sequence:

>1221_bases
ATGAAAATTGCAGTCCTCGGCGGTGACGGTTTCGTCGGCTGGCCCACCGCCCTGCATTTATCCGATGCGGGCCACGACAT
TCACATCCTCGACAATCTCTCCCGTCGCTGGATCGACACCGAACTCGGCGTGCAATCCTTGACACCGATGGATTCCATCC
AGGAGCGCACGCGCATCTGGCATGCGGAAACGGGCCGGCGCATCCACTTCAAGCTAATCGATCTCGCCCGCGACTACGAA
CTTTTGAAGAACTGGCTCGCGGAGCATCGCCCCGATGCGATCGTCCATTTCGCCGAGCAGCGGGCCGCCCCCTATTCGAT
GAAGAGCGACCGGCACAAGAATTATACGGTCAACAACAATGTCAATGCGACTCACAACCTCCTGAATGCGCTGGTCGAAC
TCGAGCTGGACGCCCATCTCGTTCATCTTGGGACAATGGGCGTCTACGGCTATTCCACCATTGGCGCGGCGATTCCCGAA
GGCTACCTGGCGGTCGGCATCGAAACCATGGGCGGCGATACGGTAAGCCAGGAAATTCTCTATCCGTCCAATCCGGGTTC
CGTCTACCATATGACCAAGTGCCTGGATCAGCTGCTTTTTCAGTTCTACGCCAAAAATGACGGGCTCAGGATCACCGACC
TGCACCAGGGCATCGTCTGGGGCACGCATACCGAGCAGACGCGGCGTCATCCCCAACTCATAAACCGCTTCGACTACGAC
GGCGATTACGGAACCGTGCTCAACCGCTTCCTGATCCAGGCGGCAATCGACTACCCGCTGACGGTTCACGGAACCGGCGG
CCAGACGCGCGCCTTCATCCATATTCAAGACTCGGTCCGCTGCATCGAACTCGCACTCAATAACCCGCCGGCACGCGGCA
ACCGCGTCGAGATATTCAATCAGATGACGGAAACGCATCGCGTCCGCGACCTCGCCGGGATGATCGCGCGCATGACCGGC
GCGAAGATCGCCTGGCTGCCCAATCCGCGCAAGGAAGCGGCGGAAAACGAGCTCGTGGTGCACAATGAGAAATTCCTGGC
GCTCGGCCTCAACCCGGTACGGCTGGAAGACGGGCTGCTTTCGGAAATTGTCGACGTGGCGAAAAAATTCGCCTATCGCG
TCGACCGCTCGCGCGTGCCGGCTGTTTCCGCCTGGACGAAGGACATCGCTCCGTTGATCAACCACGATCCGGAGGGCAAG
CGGCTGAAATCCGTTTCATGA

Upstream 100 bases:

>100_bases
TTTCCTGTGCAGGAACGAAAAGACATTTGCAAGCACCTGCAGCTTTGTCACAATCCTAATCTATCAGGACCCTGTCCTCT
TCCGATCAGGAGAATCGTGC

Downstream 100 bases:

>100_bases
GCCGCGTGTTCGCCAGTCCGGAGGGGCTTAGCCCCTCCTCTCCTGTGGCTGCACGCCACGCGTTCGTGACGCTCGTCACC
AATAGCGAGTACGCGCTCGG

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: NAD-Dependent Epimerase/Dehydratase; Sulfolipid Biosynthesis Protein; NAD Dependent Epimerase/Dehydratase Family; Sulfolipid Biosynthesis Protein SqdB; Epimerase; UDP Sulfoquinovose Synthase; Nucleoside-Diphosphate-Sugar Epimerase; NAD Dependent Epimerase/Dehydratase Family Protein

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MKIAVLGGDGFVGWPTALHLSDAGHDIHILDNLSRRWIDTELGVQSLTPMDSIQERTRIWHAETGRRIHFKLIDLARDYE
LLKNWLAEHRPDAIVHFAEQRAAPYSMKSDRHKNYTVNNNVNATHNLLNALVELELDAHLVHLGTMGVYGYSTIGAAIPE
GYLAVGIETMGGDTVSQEILYPSNPGSVYHMTKCLDQLLFQFYAKNDGLRITDLHQGIVWGTHTEQTRRHPQLINRFDYD
GDYGTVLNRFLIQAAIDYPLTVHGTGGQTRAFIHIQDSVRCIELALNNPPARGNRVEIFNQMTETHRVRDLAGMIARMTG
AKIAWLPNPRKEAAENELVVHNEKFLALGLNPVRLEDGLLSEIVDVAKKFAYRVDRSRVPAVSAWTKDIAPLINHDPEGK
RLKSVS

Sequences:

>Translated_406_residues
MKIAVLGGDGFVGWPTALHLSDAGHDIHILDNLSRRWIDTELGVQSLTPMDSIQERTRIWHAETGRRIHFKLIDLARDYE
LLKNWLAEHRPDAIVHFAEQRAAPYSMKSDRHKNYTVNNNVNATHNLLNALVELELDAHLVHLGTMGVYGYSTIGAAIPE
GYLAVGIETMGGDTVSQEILYPSNPGSVYHMTKCLDQLLFQFYAKNDGLRITDLHQGIVWGTHTEQTRRHPQLINRFDYD
GDYGTVLNRFLIQAAIDYPLTVHGTGGQTRAFIHIQDSVRCIELALNNPPARGNRVEIFNQMTETHRVRDLAGMIARMTG
AKIAWLPNPRKEAAENELVVHNEKFLALGLNPVRLEDGLLSEIVDVAKKFAYRVDRSRVPAVSAWTKDIAPLINHDPEGK
RLKSVS
>Mature_406_residues
MKIAVLGGDGFVGWPTALHLSDAGHDIHILDNLSRRWIDTELGVQSLTPMDSIQERTRIWHAETGRRIHFKLIDLARDYE
LLKNWLAEHRPDAIVHFAEQRAAPYSMKSDRHKNYTVNNNVNATHNLLNALVELELDAHLVHLGTMGVYGYSTIGAAIPE
GYLAVGIETMGGDTVSQEILYPSNPGSVYHMTKCLDQLLFQFYAKNDGLRITDLHQGIVWGTHTEQTRRHPQLINRFDYD
GDYGTVLNRFLIQAAIDYPLTVHGTGGQTRAFIHIQDSVRCIELALNNPPARGNRVEIFNQMTETHRVRDLAGMIARMTG
AKIAWLPNPRKEAAENELVVHNEKFLALGLNPVRLEDGLLSEIVDVAKKFAYRVDRSRVPAVSAWTKDIAPLINHDPEGK
RLKSVS

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 45694; Mature: 45694

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVLGGDGFVGWPTALHLSDAGHDIHILDNLSRRWIDTELGVQSLTPMDSIQERTRIW
CEEEEECCCCCCCCCEEEEECCCCCEEEEEHHHHHHHCCHHCCHHHCCCHHHHHHHHHEE
HAETGRRIHFKLIDLARDYELLKNWLAEHRPDAIVHFAEQRAAPYSMKSDRHKNYTVNNN
ECCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCCCCEEECCC
VNATHNLLNALVELELDAHLVHLGTMGVYGYSTIGAAIPEGYLAVGIETMGGDTVSQEIL
CCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCEE
YPSNPGSVYHMTKCLDQLLFQFYAKNDGLRITDLHQGIVWGTHTEQTRRHPQLINRFDYD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCHHHHHCHHHHHHCCCC
GDYGTVLNRFLIQAAIDYPLTVHGTGGQTRAFIHIQDSVRCIELALNNPPARGNRVEIFN
CCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHHHH
QMTETHRVRDLAGMIARMTGAKIAWLPNPRKEAAENELVVHNEKFLALGLNPVRLEDGLL
HHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHCCCCEEEECCEEEEECCCCEECCCHHH
SEIVDVAKKFAYRVDRSRVPAVSAWTKDIAPLINHDPEGKRLKSVS
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKIAVLGGDGFVGWPTALHLSDAGHDIHILDNLSRRWIDTELGVQSLTPMDSIQERTRIW
CEEEEECCCCCCCCCEEEEECCCCCEEEEEHHHHHHHCCHHCCHHHCCCHHHHHHHHHEE
HAETGRRIHFKLIDLARDYELLKNWLAEHRPDAIVHFAEQRAAPYSMKSDRHKNYTVNNN
ECCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCCCCEEECCC
VNATHNLLNALVELELDAHLVHLGTMGVYGYSTIGAAIPEGYLAVGIETMGGDTVSQEIL
CCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCEE
YPSNPGSVYHMTKCLDQLLFQFYAKNDGLRITDLHQGIVWGTHTEQTRRHPQLINRFDYD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCHHHHHCHHHHHHCCCC
GDYGTVLNRFLIQAAIDYPLTVHGTGGQTRAFIHIQDSVRCIELALNNPPARGNRVEIFN
CCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHHHH
QMTETHRVRDLAGMIARMTGAKIAWLPNPRKEAAENELVVHNEKFLALGLNPVRLEDGLL
HHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHCCCCEEEECCEEEEECCCCEECCCHHH
SEIVDVAKKFAYRVDRSRVPAVSAWTKDIAPLINHDPEGKRLKSVS
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA