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The map label for this gene is purA [H]

Identifier: 150397814

GI number: 150397814

Start: 2712699

End: 2713997

Strand: Reverse

Name: purA [H]

Synonym: Smed_2616

Alternate gene names: 150397814

Gene position: 2713997-2712699 (Counterclockwise)

Preceding gene: 150397816

Following gene: 150397813

Centisome position: 71.76

GC content: 63.13

Gene sequence:

>1299_bases
ATGACGAATGTCGTGGTGGTCGGATCCCAGTGGGGCGACGAAGGCAAAGGCAAGATCGTGGATTGGCTCTCGGAGCGCGC
CGATATCGTGGTGCGCTTCCAGGGCGGCCACAATGCCGGCCACACGCTGGTGATCGACGGCGTCAGCTACAAGCTGTCGC
TGCTGCCCTCGGGCGTCGTCCGCTCCGGCAAGCTCGCCGTCATCGGCAATGGTGTGGTGATCGACCCCCATGCACTGATT
GCCGAAATCGACAGGCTTGCGGCGCAGGGGGTCACTGTTACGCCCCAAAACCTGCGCATTGCCGATAATGCCACGCTCAT
TCTTTCGCTCCACCGCGAGCTCGACGGTATCCGCGAGGATGCCGCGTCGAACAGCGGCACCAAGATCGGAACGACGCGCC
GTGGCATCGGCCCGGCCTATGAGGACAAGGTCGGCCGCCGCGCAATTCGCGTCATGGATCTCGCCGATCTCGACACTCTG
CCGGCCAAGGTCGACCGGCTGCTGACGCATCACAATGCGCTGCGCCGCGGTCTTGGGGAGGCGGAAATCAGCCACCGGGC
GATCATGGACGAATTGTCGTCGGTCGCCGCGCGCGTACTGCCCTTCATGGATACGATCTGGCTGCTCCTCGACCGGGAGC
GTCGCAAGGGTGCCCGGATCCTCTTCGAAGGGGCACAGGGCACGCTGCTCGACATAGATCACGGCACTTATCCTTTCGTG
ACCTCGTCGAACACGGTCGCAGGTCAGGCTGCTGCGGGCTCGGGCATGGGCCCCGGCTCGCTCGGCTACATTCTCGGCAT
CACCAAGGCCTACACGACGCGCGTCGGTGAAGGGCCGTTCCCGACCGAGCTCGACGACGAGGTCGGCCGCTTTCTTGGTG
AGCGCGGCCATGAATTCGGGACCGTTACGGGGCGCAAGCGTCGGTGCGGCTGGTTCGATGCTGCTCTGGTGCGCCAGTCG
GTCGCCGCCAACGGCATCACGGGCATAGCACTTACCAAGCTCGACGTGCTCGACGGACTCGACGAGTTGAAGATTTGCGT
CGGTTATACTCTGGATGGACAGCAGATCGACCATCTTCCCGCAAGTCAAGCGCAGCAAGCTCTGGCAAAGCCGGTCTACA
TCACGCTCGAAGGATGGAAAGAATCGACGGTTGGTGCGCGCAGTTGGGCCGAACTTCCGGCGCAGGCGATCAAATATGTT
CGCCAGGTCGAGGAACTGATCGGCGCGCCGGTCGCGCTACTTTCGACCAGCCCGGAACGGGACGATACGATACTTGTGAC
GGACCCTTTTGAGGACTAG

Upstream 100 bases:

>100_bases
GCTCATAACGGGGTCGCTTTCCGAAAGACAACACAGTATAGATGCGCGGGTTTTGAGAACAGCGCACCTTCCGCATGCGC
GCGTGAACAGGAAGAGAATT

Downstream 100 bases:

>100_bases
TGTCGGCCCAGGGAAAGCGGCGACGCGGCAATTGCCGCGGGTCTGGAAACTGAGAAAGTACTGATGGCGGATTTTGTTGC
AGTTATTCGCAGGACGGTAG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTNVVVVGSQWGDEGKGKIVDWLSERADIVVRFQGGHNAGHTLVIDGVSYKLSLLPSGVVRSGKLAVIGNGVVIDPHALI
AEIDRLAAQGVTVTPQNLRIADNATLILSLHRELDGIREDAASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADLDTL
PAKVDRLLTHHNALRRGLGEAEISHRAIMDELSSVAARVLPFMDTIWLLLDRERRKGARILFEGAQGTLLDIDHGTYPFV
TSSNTVAGQAAAGSGMGPGSLGYILGITKAYTTRVGEGPFPTELDDEVGRFLGERGHEFGTVTGRKRRCGWFDAALVRQS
VAANGITGIALTKLDVLDGLDELKICVGYTLDGQQIDHLPASQAQQALAKPVYITLEGWKESTVGARSWAELPAQAIKYV
RQVEELIGAPVALLSTSPERDDTILVTDPFED

Sequences:

>Translated_432_residues
MTNVVVVGSQWGDEGKGKIVDWLSERADIVVRFQGGHNAGHTLVIDGVSYKLSLLPSGVVRSGKLAVIGNGVVIDPHALI
AEIDRLAAQGVTVTPQNLRIADNATLILSLHRELDGIREDAASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADLDTL
PAKVDRLLTHHNALRRGLGEAEISHRAIMDELSSVAARVLPFMDTIWLLLDRERRKGARILFEGAQGTLLDIDHGTYPFV
TSSNTVAGQAAAGSGMGPGSLGYILGITKAYTTRVGEGPFPTELDDEVGRFLGERGHEFGTVTGRKRRCGWFDAALVRQS
VAANGITGIALTKLDVLDGLDELKICVGYTLDGQQIDHLPASQAQQALAKPVYITLEGWKESTVGARSWAELPAQAIKYV
RQVEELIGAPVALLSTSPERDDTILVTDPFED
>Mature_431_residues
TNVVVVGSQWGDEGKGKIVDWLSERADIVVRFQGGHNAGHTLVIDGVSYKLSLLPSGVVRSGKLAVIGNGVVIDPHALIA
EIDRLAAQGVTVTPQNLRIADNATLILSLHRELDGIREDAASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADLDTLP
AKVDRLLTHHNALRRGLGEAEISHRAIMDELSSVAARVLPFMDTIWLLLDRERRKGARILFEGAQGTLLDIDHGTYPFVT
SSNTVAGQAAAGSGMGPGSLGYILGITKAYTTRVGEGPFPTELDDEVGRFLGERGHEFGTVTGRKRRCGWFDAALVRQSV
AANGITGIALTKLDVLDGLDELKICVGYTLDGQQIDHLPASQAQQALAKPVYITLEGWKESTVGARSWAELPAQAIKYVR
QVEELIGAPVALLSTSPERDDTILVTDPFED

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=423, Percent_Identity=43.7352245862884, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI34577063, Length=425, Percent_Identity=43.0588235294118, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=41.8781725888325, Blast_Score=313, Evalue=2e-85,
Organism=Escherichia coli, GI1790620, Length=432, Percent_Identity=50.2314814814815, Blast_Score=433, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI25146553, Length=437, Percent_Identity=42.1052631578947, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI25146555, Length=434, Percent_Identity=41.705069124424, Blast_Score=333, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6324109, Length=432, Percent_Identity=39.8148148148148, Blast_Score=315, Evalue=6e-87,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=42.4528301886792, Blast_Score=333, Evalue=1e-91,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46432; Mature: 46301

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNVVVVGSQWGDEGKGKIVDWLSERADIVVRFQGGHNAGHTLVIDGVSYKLSLLPSGVV
CCCEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCEEEEECCCCCC
RSGKLAVIGNGVVIDPHALIAEIDRLAAQGVTVTPQNLRIADNATLILSLHRELDGIRED
CCCCEEEEECCEEECCHHHHHHHHHHHHCCCEECCCCEEEECCCEEEEEEHHHHHHHHHH
AASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADLDTLPAKVDRLLTHHNALRRGLGE
HCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEHHCCCHHHHHHHHHHHHHHHHHHCCCH
AEISHRAIMDELSSVAARVLPFMDTIWLLLDRERRKGARILFEGAQGTLLDIDHGTYPFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCEE
TSSNTVAGQAAAGSGMGPGSLGYILGITKAYTTRVGEGPFPTELDDEVGRFLGERGHEFG
ECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCC
TVTGRKRRCGWFDAALVRQSVAANGITGIALTKLDVLDGLDELKICVGYTLDGQQIDHLP
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCEEEEEEEEECCCCCCCCC
ASQAQQALAKPVYITLEGWKESTVGARSWAELPAQAIKYVRQVEELIGAPVALLSTSPER
HHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
DDTILVTDPFED
CCEEEEECCCCC
>Mature Secondary Structure 
TNVVVVGSQWGDEGKGKIVDWLSERADIVVRFQGGHNAGHTLVIDGVSYKLSLLPSGVV
CCEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEEECCCEEEEECCCCCC
RSGKLAVIGNGVVIDPHALIAEIDRLAAQGVTVTPQNLRIADNATLILSLHRELDGIRED
CCCCEEEEECCEEECCHHHHHHHHHHHHCCCEECCCCEEEECCCEEEEEEHHHHHHHHHH
AASNSGTKIGTTRRGIGPAYEDKVGRRAIRVMDLADLDTLPAKVDRLLTHHNALRRGLGE
HCCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEHHCCCHHHHHHHHHHHHHHHHHHCCCH
AEISHRAIMDELSSVAARVLPFMDTIWLLLDRERRKGARILFEGAQGTLLDIDHGTYPFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCEE
TSSNTVAGQAAAGSGMGPGSLGYILGITKAYTTRVGEGPFPTELDDEVGRFLGERGHEFG
ECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCC
TVTGRKRRCGWFDAALVRQSVAANGITGIALTKLDVLDGLDELKICVGYTLDGQQIDHLP
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCEEEEEEEEECCCCCCCCC
ASQAQQALAKPVYITLEGWKESTVGARSWAELPAQAIKYVRQVEELIGAPVALLSTSPER
HHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
DDTILVTDPFED
CCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]